Metallicity of Red Giants in the Galactic Bulge from Near-Infrared Spectroscopy

We present K-band spectra of more than 110 M giants in Galactic bulge fields interior to -4 degrees and as close as 0.2 degrees of the Galactic Center. From the equivalent widths of three features in these spectra, EW(Na),EW(Ca), and EW(CO) we calculate [Fe/H] for the stars with a calibration derived from globular clusters Stephens et al (2000). The mean [Fe/H] for each field is in good agreement with the results from Frogel et al. (1999) based on the slope of the giant branch method. We find no evidence for a metallicity gradient along the minor or major axes of the inner bulge (R < 0.6 kpc). A metallicity gradient along the minor axis, found earlier, arises when fields located at larger galactic radius are included. However, these more distant fields are located outside of the infrared bulge defined by the COBE/DIRBE observations. We compute the [Fe/H] distribution for the inner bulge and find a mean value of -0.21 dex with a full width dispersion of 0.30 dex, close to the values found for Baade's Window (BW) by Sadler et al. (1996) and to a theoretical prediction for a bulge formed by dissipative collapse Molla et al (2000).Comment: 32 pages, 10 figures, AJ submitte

The HR image slicer for GNIRS at Gemini North: optical design and performance

GNIRS (Gemini Near-InfraRed Spectrograph) is a multi-function spectrograph at Gemini North telescope offering four observational modes in the spectral range of 0.8 to 5.4 µm. It provides 2-pixel spectral resolutions from 1,200 up to 18,0000 and has single disperser and cross-disperser modes yielding simultaneous spectral bandwidths from 40 nm to 1,650 nm. GNIRS presented three existing modes: long-slit (50-100" slit), cross-dispersed (5-7" slit) and low resolution (LR) Integral Field Unit (IFU) (3.15" x 4.80") and it is now being upgraded with a fourth mode allowing high resolution (HR) IFU spectroscopy using an image slicer optimised for fully adaptively corrected images over a field of view of 2.25 arcsec2 (1.80" x 1.25") covered by 25 slices of 410 µm width offering a spatial sampling of 0.05 x 0.05 arscec2 with a diffraction limited optical quality. The proposed layout meets specifications and some challenging design constraints: it shall be contained within the same envelope defined by the LR image slicer (0.1 x 0.2 x 0.1 m3 ), the input and output focal-ratios of both image slicers shall be the same and at exact positions but providing different anamorphic magnifications and preserving the optical quality. The length of the generated slit will be similar to the length of the slit in long-slit mode to maximise detector use and avoid vignetting. This communication presents the optical design and performance of the high resolution image slicer compliant with all specifications and constraints and it shows some design adaptations adopted in order to facilitate its manufacturing in metal at Durham University

Limits on Quaoar's Atmosphere

Here we present high cadence photometry taken by the Acquisition Camera on Gemini South, of a close passage by the ~540 km radius Kuiper belt object, (50000) Quaoar, of a r' = 20.2 background star. Observations before and after the event show that the apparent impact parameter of the event was 0."019 ± 0."004, corresponding to a close approach of 580 ± 120 km to the center of Quaoar. No signatures of occultation by either Quaoar's limb or its potential atmosphere are detectable in the relative photometry of Quaoar and the target star, which were unresolved during closest approach. From this photometry we are able to put constraints on any potential atmosphere Quaoar might have. Using a Markov chain Monte Carlo and likelihood approach, we place pressure upper limits on sublimation supported, isothermal atmospheres of pure N_2, CO, and CH_4. For N_2 and CO, the upper limit surface pressures are 1 and 0.7 μbar, respectively. The surface temperature required for such low sublimation pressures is ~33 K, much lower than Quaoar's mean temperature of ~44 K measured by others. We conclude that Quaoar cannot have an isothermal N_2 or CO atmosphere. We cannot eliminate the possibility of a CH_4 atmosphere, but place upper surface pressure and mean temperature limits of ~138 nbar and ~44 K, respectively

The birth place of the type Ic Supernova 2007gr

We report our attempts to locate the progenitor of the peculiar type Ic SN 2007gr in HST pre-explosion images of the host galaxy, NGC 1058. Aligning adaptive optics Altair/NIRI imaging of SN 2007gr from the Gemini (North) Telescope with the pre-explosion HST WFPC2 images, we identify the SN position on the HST frames with an accuracy of 20 mas. Although nothing is detected at the SN position we show that it lies on the edge of a bright source, 134+/-23 mas (6.9 pc) from its nominal centre. Based on its luminosity we suggest that this object is possibly an unresolved, compact and coeval cluster and that the SN progenitor was a cluster member, although we note that model profile fitting favours a single bright star. We find two solutions for the age of this assumed cluster; 7-/+0.5 Myrs and 20-30 Myrs, with turn-off masses of 28+/-4 Msun and 12-9 Msun respectively. Pre-explosion ground-based K-band images marginally favour the younger cluster age/higher turn-off mass. Assuming the SN progenitor was a cluster member, the turn-off mass provides the best estimate for its initial mass. More detailed observations, after the SN has faded, should determine if the progenitor was indeed part of a cluster, and if so allow an age estimate to within ~2 Myrs thereby favouring either a high mass single star or lower mass interacting binary progenitor.Comment: 12 pages, 3 figures, resolution of fig 1. has been reduced, some revision based on referee's comments, Accepted ApJL 27 Nov 200

Di-cationic arylimidamides Act Against Neospora caninum tachyzoites by Interference in Membrane Structure and Nucleolar Integrity and are Active Against Challenge Infection in Mice

Neospora caninum is considered to be the main cause of bovine abortion in Europe and the USA, leading to considerable financial impact. Losses are caused directly by abortions or indirectly through breeding of calves with impaired viability. Due to the lack of effective chemotherapy against bovine neosporosis, there is a need to develop new anti-protozoal compounds, which would either eliminate the parasite or avoid its transmission. In order to identify compounds of interest, the in vitro activities of 41 di-cationic pentamidine derivatives were studied employing a transgenic N. caninum clone expressing beta-galactosidase as a reporter gene. The arylimidamide DB745, previously shown to be highly active against Leishmania donovani in vitro and in vivo, appeared as the most promising compound, with an IC50 of 80 nM in 3-day growth assays and severely affecting both host cell invasion as well as intracellular proliferation. TEM of intracellular tachyzoites identified distinct alterations related to the nucleolus and the nuclear and cellular membrane. Long-term growth assays showed that DB745 acted parasiticidal upon the Nc-Liv isolate, but not against the Nc-1 isolate of N. caninum. In vivo studies in N. caninum (Nc-1 isolate) infected mice showed that daily intraperitoneal application of DB745 for a period of 14 days resulted in a decreased number of clinically affected animals, and lower cerebral parasite burdens in DB745-treated mice compared to non-treated mice. These results illustrate the potential of dicationic arylimidamides for the treatment of N. caninum infections

The adaptive potential of a survival artist: characterization of the in vitro interactions of Toxoplasma gondii tachyzoites with di-cationic compounds in human fibroblast cell cultures

The impact of di-cationic pentamidine-analogues against Toxoplama gondii (Rh- and Me49-background) was investigated. The 72 h-growth assays showed that the arylimidamide DB750 inhibited the proliferation of tachyzoites of T. gondii Rh and T. gondii Me49 with an IC50 of 0·11 and 0·13 μm, respectively. Pre-incubation of fibroblast monolayers with 1 μm DB750 for 12 h and subsequent culture in the absence of the drug also resulted in a pronounced inhibiton of parasite proliferation. However, upon 5-6 days of drug exposure, T. gondii tachyzoites adapted to the compound and resumed proliferation up to a concentration of 1·2 μm. Out of a set of 32 di-cationic compounds screened for in vitro activity against T. gondii, the arylimidamide DB745, exhibiting an IC50 of 0·03 μm and favourable selective toxicity was chosen for further studies. DB745 also inhibited the proliferation of DB750-adapted T. gondii (IC50=0·07 μm). In contrast to DB750, DB745 also had a profound negative impact on extracellular non-adapted T. gondii tachyzoites, but not on DB750-adapted T. gondii. Adaptation of T. gondii to DB745 (up to a concentration of 0·46 μm) was much more difficult to achieve and feasible only over a period of 110 days. In cultures infected with DB750-adapted T. gondii seemingly intact parasites could occasionally be detected by TEM. This illustrates the astonishing capacity of T. gondii tachyzoites to adapt to environmental changes, at least under in vitro conditions, and suggests that DB745 could be an interesting drug candidate for further assessments in appropriate in vivo model

The adaptive potential of a survival artist: characterization of the in vitro interactions of Toxoplasma gondii tachyzoites with di-cationic compounds in human fibroblast cell cultures

The impact of di-cationic pentamidine-analogues against Toxoplama gondii (Rh- and Me49-background) was investigated. The 72 h-growth assays showed that the arylimidamide DB750 inhibited the proliferation of tachyzoites of T. gondii Rh and T. gondii Me49 with an IC50 of 0·11 and 0·13 μm, respectively. Pre-incubation of fibroblast monolayers with 1 μm DB750 for 12 h and subsequent culture in the absence of the drug also resulted in a pronounced inhibiton of parasite proliferation. However, upon 5-6 days of drug exposure, T. gondii tachyzoites adapted to the compound and resumed proliferation up to a concentration of 1·2 μm. Out of a set of 32 di-cationic compounds screened for in vitro activity against T. gondii, the arylimidamide DB745, exhibiting an IC50 of 0·03 μm and favourable selective toxicity was chosen for further studies. DB745 also inhibited the proliferation of DB750-adapted T. gondii (IC50=0·07 μm). In contrast to DB750, DB745 also had a profound negative impact on extracellular non-adapted T. gondii tachyzoites, but not on DB750-adapted T. gondii. Adaptation of T. gondii to DB745 (up to a concentration of 0·46 μm) was much more difficult to achieve and feasible only over a period of 110 days. In cultures infected with DB750-adapted T. gondii seemingly intact parasites could occasionally be detected by TEM. This illustrates the astonishing capacity of T. gondii tachyzoites to adapt to environmental changes, at least under in vitro conditions, and suggests that DB745 could be an interesting drug candidate for further assessments in appropriate in vivo model

Impaired coordination of nutrient intake and substrate oxidation in melanocortin-4 receptor knockout mice

Mutations in the melanocortin-4 receptor (MC4R) are associated with obesity. The obesity syndrome observed in humans with MC4R haploinsufficiency is similar to that observed in MC4R knockout mice: increased longitudinal growth, hyperphagia, and fasting hyperinsulinemia. For comparison with other commonly investigated models of obesity and insulin resistance, we have backcrossed Mc4r-/- mice into the C57BL/6J (B6) background. Female obese Mc4r-/- mice exhibit reduced energy expenditure and an attenuated increase in fatty acid oxidation following exposure to high fat diets compared to obese Lep ob/Lepob mice. The reduced energy expenditure and fatty acid oxidation correlates with changes in hepatic gene expression. The expression of genes involved in fatty acid oxidation increased in obese Lep ob/Lepob mice compared to wild type and obese Mc4r-/- mice. In contrast, a key lipogenic enzyme (fatty acid synthase) is increased in obese Mc4r-/- mice compared to obese Lepob/Lepob mice. Hyperinsulinemia, increased FAS mRNA expression and hepatic steatosis appear to be secondary to obesity in B6 Mc4r-/- mice. However, Mc4r-/- mice in a mixed genetic background develop severe hepatic steatosis at an early age. This might suggest an important role of the MC4R in regulating liver fatty acid metabolism this is masked on the B6 background. Interestingly, the 10- to 20-fold increase in liver triglyceride in this strain of Mc4r-/- mice is not always associated with fasting hyperinsulinemia or increased FAS mRNA expression. This observation suggests changes in liver secondary to triglyceride accumulation lead to hyperinsulinemia and increased hepatic FAS expression in Mc4r-/- mice