Resultados del tratamiento de la inestabilidad postdisectomia mediante artrodesis posterolateral lumbar

El mecanismo fisiopatológico por el cual, tras una discectomia en columna lumbar, se puede producir inestabilidad en este segmento de la columna, ha sido establecido en múltiples estudios y se encuentra en la bibliografía actual. Sin embargo, hay pocas publicaciones en la literatura que hagan referencia a los resultados de la artrodesis posterolateral lumbar en inestabilidad clínico-radiológica generada tras una discectomia lumbar. El propósito del presente estudio es valorar los resultados obtenidos en mejora de calidad de vida, en pacientes sometidos a artrodesis lumbar con tornillos pediculares en inestabilidad postdisectomía. La población estudiada se trató de 15 pacientes, 10 hombres y 5 mujeres con edades comprendidas entre los 27 y 65 años, la selección de los pacientes además del antecedente de cirugía discal, se basó en la presencia de un cuadro clínico de inestabilidad unido a pruebas radiológicas estáticas, dinámicas y resonancia magnética, y por último la ausencia de problemas de tipo laboral o psicológico, los datos se obtuvieron por entrevista personal y llamada telefónica, utilizando la escala de Oswestry y la escala analógica de Scott. Los resultados obtenidos fueron buenos ya que más del 90% de los pacientes refirieron mejoría en su calidad de vida. Se puede concluir que la artrodesis posterolateral con tornillos pediculares en inestabilidad postdisectomia, es una buena opción quirúrgica, con la cual se puede obtener mejoría del cuadro clínico del paciente.The present study has the aime, the evaluation of results of posterolateral lumbar arthrodesis in patients who had undergone lumbar discal surgery failed because of appearance of instability. Retrospective study in 15 patients with diagnosis instability postdiskectomy made by: clinical, static and dynamic x-ray and MRI, treated with posterolateral lumbar arthrodesis using pedicular screw. Ten males and five female of 27-65 years and average 46, we used the life quality scale Oswestry and Scott scale. The shortest follow up 3 years and longest 6 years. Our results were: time between diskectomy and arthrodesis variated from 1 to 10 years, average 5 years, about: pain intensity, patients need analgesic because pain, help for activities, deambulation, sexual activity all of them improve. We had 3 cases with clinical symptoms, the other one pain free. One case of deep infection of wound where surgical debridement was necessary. Finally Scott¿s scale prior to surgery punctuation was between 1-4. after between 8-10. Excepting 1 case with bad evolution. Conclusion: posterolateral lumbar arthrodesis with pedicular screew can be a good option of treatment in instability postdikectomy

Registro fotográfico de un murciélago capturado por Leopardus pardalis (Carnivora: Felidae) en la Reserva de la Biosfera de Calakmul, México

The ocelot faces conservation problems throughout its distribution, mainly due to illegal hunting and habitat transformation. It has been documented that the ocelot diet consists mainly of small nocturnal terrestrial mammals, especially rodents, although it has also been recorded that they can feed on possums, squirrels, rabbits, primates, iguanas, frogs, birds, fish, insects and terrestrial crabs.This note is relevant to the feeding behavior of ocelots in Mexico because it describes a photographic event of an ocelot feeding on a bat, the record was obtained with camera traps placed in the southern core zone of the Calakmul Biosphere Reserve. These cameras were installed to record that species of fauna visitrificiales drinkers (water sources constantly supplied by members of the project) that were placed as part of a pilot project in the years 2018 and 2019 and aim to supply water in the RBC for use of wildlife. As a result of this effort, this photographic record was obtained of an ocelot individual feeding on a bat associated with an artificial drinker.El ocelote enfrenta problemas de conservación a lo largo de su distribución, debido principalmente a la caza ilegal y transformación del hábitat. Se ha documentado que la dieta del ocelote consiste principalmente de pequeños mamíferos terrestres nocturnos, especialmente roedores , aunque también se ha registrado que pueden alimentarse de zarigüeyas, ardillas, conejos, primates, iguanas, ranas, aves, peces, insectos y cangrejos terrestres. La presente nota resulta relevante sobre el comportamiento alimentario de los ocelotes en Mexico debido a que se describe un evento fotográfico de un ocelote alimentándose de un murciélago, el registro fue obtenido con trampas cámara colocadas en la zona nucleo sur de la Reserva de la Biosfera de Calakmul . Estas camaras fueron instaladas para registrar que especies de fauna visitan a los bebederos ariticiales (fuentes de agua abastecidas constantemente por miembros del proyecto) que fueron colocados como parte de un proyecto piloto en los años 2018 y 2019 y tienen como objetivo el abastecimiento de agua en la RBC para uso de la fauna silvestre. Cómo resultado de este esfuerzo, se obtuvo el presente registro fotográfico de un individuo de ocelote alimentandose de un murcielago asociado a un bebedero artificial. &nbsp

Estudio para la mejora de la calidad del grado en Trabajo Social en atención a las necesidades de nuestros egresados y sus empleadores

Mediante este proyecto la Facultad de Trabajo Social de la UCM pretende conocer cuáles son las necesidades formativas de nuestros alumnos con el principal objetivo de hacer una modificación en la titulación que pueda dar respuesta a las necesidades de conocimiento de nuestros profesionales así como conocer las condiciones esenciales en las que nuestros egresado acceden al mercado laboral

Uso de bebederos artificiales por el sereque centroamericano (Dasyprocta punctata) en la reserva de la biosfera de Calakmul, México

Objective: To describe the use of artificial drinking fountains by the central American Agouti (Dasyprocta punctata) in the Calakmul Biosphere Reserve. Design/methodology/approach: Eight artificial drinking fountains were monitored each one with a trap camera with the aim to estimate the relative abundance index and activity patterns of visits to the drinking fountains. The monitoring was during the dry season of 2019. The analyzes were carried out with the InfoStat and R programs. Results: From January to May 2019, with a sampling effort of 1121 days/camera, average abundance of 160.08±85.44. The pattern of visits to the drinking troughs was mainly diurnal. Limitations of the study/implications: The use of trap cameras in the artificial drinking fountains allows us to know the extent of habitat modification in the Calakmul Biosphere Reserve. Findings/conclusions: The implementation of artificial drinking fountains in the RBC during the dry season was a successful action to mitigate the effects of water scarcity. The use of the water resource by the Central American Agouti in artificial drinking fountains was constant, other species of mammals and birds were also observed making use of artificial drinking fountains.Objetivo: Describir el uso de bebederos artificiales por el sereque (Dasyprocta punctata) en la Reserva de la Biosfera de Calakmul. Diseño/metodología/aproximación: Se monitorearon ocho bebederos artificiales cada uno con una cámara trampa con la finalidad de estimar el índice de abundancia relativa y los patrones de actividad de visitas a los bebederos. El monitoreo fue durante la temporada de secas del 2019. Los análisis se realizaron con los programas R e InfoStat. Resultado: De enero a mayo de 2019, con un esfuerzo de muestreo fue de 1121 días/cámara, abundancia promedio de 160.08±85.44. El patrón de visitas a los bebederos fue principalmente diurno. Limitaciones/implicaciones: El uso de cámaras trampa en los bebederos artificiales nos permite conocer los alcances en la modificación del hábitat en la Reserva de la Biosfera de Calakmul (RBC). Hallazgos/conclusiones: La implementación de bebederos artificiales en la RBC durante la temporada de seca fue una acción de éxito para mitigar los efectos de la escases de agua. El aprovechamiento del recurso agua por el sereque en los bebederos artificiales fue constante, también se observaron otras especies de mamíferos y aves haciendo uso de los bebederos artificiales

The Thyroid Hormone Receptors Modulate the Skin Response to Retinoids

[Background]: Retinoids play an important role in skin homeostasis and when administered topically cause skin hyperplasia, abnormal epidermal differentiation and inflammation. Thyroidal status in humans also influences skin morphology and function and we have recently shown that the thyroid hormone receptors (TRs) are required for a normal proliferative response to 12-O-tetradecanolyphorbol-13-acetate (TPA) in mice. [Methodology/Principal Findings]: We have compared the epidermal response of mice lacking the thyroid hormone receptor binding isoforms TRα1 and TRβ to retinoids and TPA. Reduced hyperplasia and a decreased number of proliferating cells in the basal layer in response to 9-cis-RA and TPA were found in the epidermis of TR-deficient mice. Nuclear levels of proteins important for cell proliferation were altered, and expression of keratins 5 and 6 was also reduced, concomitantly with the decreased number of epidermal cell layers. In control mice the retinoid (but not TPA) induced parakeratosis and diminished expression of keratin 10 and loricrin, markers of early and terminal epidermal differentiation, respectively. This reduction was more accentuated in the TR deficient animals, whereas they did not present parakeratosis. Therefore, TRs modulate both the proliferative response to retinoids and their inhibitory effects on skin differentiation. Reduced proliferation, which was reversed upon thyroxine treatment, was also found in hypothyroid mice, demonstrating that thyroid hormone binding to TRs is required for the normal response to retinoids. In addition, the mRNA levels of the pro-inflammatory cytokines TNFα and IL-6 and the chemotactic proteins S1008A and S1008B were significantly elevated in the skin of TR knock-out mice after TPA or 9-cis-RA treatment and immune cell infiltration was also enhanced. [Conclusions/significance]: Since retinoids are commonly used for the treatment of skin disorders, these results demonstrating that TRs regulate skin proliferation, differentiation and inflammation in response to these compounds could have not only physiological but also therapeutic implications.This work was supported by grants BFU2007-62402 and SAF2008-00121 from Ministerio de Ciencia e Innovación, RD06/0020/0036 and RD06/0020/0029 from the Fondo de Investigaciones Sanitarias and by the European Grant CRESCENDO (FP-018652).Peer reviewe

P27Kip1, regulated by glycogen synthase kinase-3β, results in HMBA-induced differentiation of human gastric cancer cells

<p>Abstract</p> <p>Background</p> <p>Gastric cancer is the second most common cause of global cancer-related mortality. Although dedifferentiation predicts poor prognosis in gastric cancer, the molecular mechanism underlying dedifferentiation, which could provide fundamental insights into tumor development and progression, has yet to be elucidated. Furthermore, the molecular mechanism underlying the effects of hexamethylene bisacetamide (HMBA), a recently discovered differentiation inducer, requires investigation and there are no reported studies concerning the effect of HMBA on gastric cancer.</p> <p>Methods</p> <p>Based on the results of FACS analysis, the levels of proteins involved in the cell cycle or apoptosis were determined using western blotting after single treatments and sequential combinations of HMBA and LiCl. GSK-3β and proton pump were investigated by western blotting after up-regulating Akt expression by Ad-Akt infection. To investigate the effects of HMBA on protein localization and the activities of GSK-3β, CDK2 and CDK4, kinase assays, immunoprecipitation and western blotting were performed. In addition, northern blotting and RNase protection assays were carried out to determine the functional concentration of HMBA.</p> <p>Results</p> <p>HMBA increased p27Kip1 expression and induced cell cycle arrest associated with gastric epithelial cell differentiation. In addition, treating gastric-derived cells with HMBA induced G0/G1 arrest and up-regulation of the proton pump, a marker of gastric cancer differentiation. Moreover, treatment with HMBA increased the expression and activity of GSK-3β in the nucleus but not the cytosol. HMBA decreased CDK2 activity and induced p27Kip1 expression, which could be rescued by inhibition of GSK-3β. Furthermore, HMBA increased p27Kip1 binding to CDK2, and this was abolished by GSK-3β inhibition.</p> <p>Conclusions</p> <p>The results presented herein suggest that GSK-3β functions by regulating p27Kip1 assembly with CDK2, thereby playing a critical role in G0/G1 arrest associated with HMBA-induced gastric epithelial cell differentiation.</p

Wetlands for wastewater treatment and subsequent recycling of treated effluent : a review

Due to water scarcity challenges around the world, it is essential to think about non-conventional water resources to address the increased demand in clean freshwater. Environmental and public health problems may result from insufficient provision of sanitation and wastewater disposal facilities. Because of this, wastewater treatment and recycling methods will be vital to provide sufficient freshwater in the coming decades, since water resources are limited and more than 70% of water are consumed for irrigation purposes. Therefore, the application of treated wastewater for agricultural irrigation has much potential, especially when incorporating the reuse of nutrients like nitrogen and phosphorous, which are essential for plant production. Among the current treatment technologies applied in urban wastewater reuse for irrigation, wetlands were concluded to be the one of the most suitable ones in terms of pollutant removal and have advantages due to both low maintenance costs and required energy. Wetland behavior and efficiency concerning wastewater treatment is mainly linked to macrophyte composition, substrate, hydrology, surface loading rate, influent feeding mode, microorganism availability, and temperature. Constructed wetlands are very effective in removing organics and suspended solids, whereas the removal of nitrogen is relatively low, but could be improved by using a combination of various types of constructed wetlands meeting the irrigation reuse standards. The removal of phosphorus is usually low, unless special media with high sorption capacity are used. Pathogen removal from wetland effluent to meet irrigation reuse standards is a challenge unless supplementary lagoons or hybrid wetland systems are used