Atmospheric influences on the anomalous 2016 Antarctic sea ice decay

In contrast to the Arctic, where total sea ice extent (SIE) has been decreasing for the last three decades, Antarctic SIE has shown a small, but significant, increase during the same time period. However, in 2016, an unusually early onset of the melt season was observed; the maximum Antarctic SIE was already reached as early as August rather than the end of September, and was followed by a rapid decrease. The decay was particularly strong in November, when Antarctic SIE exhibited a negative anomaly (compared to the 1979–2015 average) of approximately 2 million km2. ECMWF Interim reanalysis data showed that the early onset of the melt and the rapid decrease in sea ice area (SIA) and SIE were associated with atmospheric flow patterns related to a positive zonal wave number three (ZW3) index, i.e., synoptic situations leading to strong meridional flow and anomalously strong southward heat advection in the regions of strongest sea ice decline. A persistently positive ZW3 index from May to August suggests that SIE decrease was preconditioned by SIA decrease. In particular, in the first third of November northerly flow conditions in the Weddell Sea and the Western Pacific triggered accelerated sea ice decay, which was continued in the following weeks due to positive feedback effects, leading to the unusually low November SIE. In 2016, the monthly mean Southern Annular Mode (SAM) index reached its second lowest November value since the beginning of the satellite observations. A better spatial and temporal coverage of reliable ice thickness data is needed to assess the change in ice mass rather than ice area

Acanalonia conica (Say, 1830) and three other true hopper species new for Austria (Hemiptera: Auchenorrhyncha)

Acanalonia conica (Say, 1830) und drei weitere Zikadenarten neu für Österreich (Hemiptera: Auchenorrhyncha). – Drei Neozoen, Acanalonia conica (Say, 1830), Aplos simplex (Germar, 1830) und Hishimonus hamatus Kuoh, 1976 wurden in einer Gärtnerei in Graz erstmals für Österreich nachgewiesen. Sie wurden wahrscheinlich mit Ziersträuchern aus Italien importiert. Ein weiterer Erstnachweis betrifft Chiasmus conspurcatus (Perris, 1857). Diese Art ist heimisch und wurde an zwei Salzlacken des Nationalparks Neusiedlersee-Seewinkel nachgewiesen. Eine Gesamtliste jener 71 Zikadenarten, die an den beiden Lacken gefunden wurden, wird ebenfalls vorgelegt.  Acanalonia conica (Say, 1830), Aplos simplex (Germar, 1830) and Hishimonus hamatus Kuoh, 1976 are three alien species reported for the first time from Austria. They were found in a garden centre in Graz and presumably imported with ornamental shrubs from Italy. Another first record is Chiasmus conspurcatus (Perris, 1857), an indigenous species found in the Neusiedlersee-Seewinkel National Park. In addition, we present a list of 71 Auchenorrhyncha species found at the two inland salt marshes, where C. conspurcatus occurs

Evidence for Lignocellulose-Decomposing Enzymes in the Genome and Transcriptome of the Aquatic Hyphomycete Clavariopsis aquatica

Fungi are ecologically outstanding decomposers of lignocellulose. Fungal lignocellulose degradation is prominent in saprotrophic Ascomycota and Basidiomycota of the subkingdom Dikarya. Despite ascomycetes dominating the Dikarya inventory of aquatic environments, genome and transcriptome data relating to enzymes involved in lignocellulose decay remain limited to terrestrial representatives of these phyla. We sequenced the genome of an exclusively aquatic ascomycete (the aquatic hyphomycete Clavariopsis aquatica), documented the presence of genes for the modification of lignocellulose and its constituents, and compared differential gene expression between C. aquatica cultivated on lignocellulosic and sugar-rich substrates. We identified potential peroxidases, laccases, and cytochrome P450 monooxygenases, several of which were differentially expressed when experimentally grown on different substrates. Additionally, we found indications for the regulation of pathways for cellulose and hemicellulose degradation. Our results suggest that C. aquatica is able to modify lignin to some extent, detoxify aromatic lignin constituents, or both. Such characteristics would be expected to facilitate the use of carbohydrate components of lignocellulose as carbon and energy sources

A new 200‐year spatial reconstruction of West Antarctic surface mass balance

High‐spatial resolution surface mass balance (SMB) over the West Antarctic Ice Sheet (WAIS) spanning 1800‐2010 is reconstructed by means of ice core records combined with the outputs of the European Centre for Medium‐range Weather Forecasts “Interim” reanalysis (ERA‐Interim) and the latest polar version of the Regional Atmospheric Climate Model (RACMO2.3p2). The reconstruction reveals a significant negative trend (‐1.9 ± 2.2 Gt yr‐1 decade‐1) in the SMB over the entire WAIS during the 19th century, but a statistically significant positive trend of 5.4 ± 2.9 Gt yr‐1 decade‐1 between 1900 and 2010, in contrast to insignificant WAIS SMB changes during the 20th century reported earlier. At regional scales, the Antarctic Peninsula (AP) and western WAIS show opposite SMB trends, with different signs in the 19th and 20th centuries. The annual resolution reconstruction allows us to examine the relationships between SMB and large‐scale atmospheric oscillations. Although SMB over the AP and western WAIS correlates significantly with the Southern Annular Mode (SAM) due to the influence of the Amundsen Sea Low (ASL) and El Niño/Southern Oscillation (ENSO) during 1800‐2010, the significant correlations are temporally unstable, associated with the phase of SAM, ENSO and the Pacific decadal oscillation (PDO). In addition, the two climate modes seem to contribute little to variability in SMB over the whole WAIS on decadal‐centennial time scales. This new reconstruction also serves to identify unreliable precipitation trends in ERA‐Interim, and thus has potential for assessing the skill of other reanalyses or climate models to capture precipitation trends and variability

A comparison of Antarctic ice sheet surface mass balance from atmospheric climate models and in situ observations

In this study, 3265 multiyear averaged in situ observations and 29 observational records at annual time scale are used to examine the performance of recent reanalysis and regional atmospheric climate model products [ERA-Interim, JRA-55, MERRA, the Polar version of MM5 (PMM5), RACMO2.1, and RACMO2.3] for their spatial and interannual variability of Antarctic surface mass balance (SMB), respectively. Simulated precipitation seasonality is also evaluated using three in situ observations and model intercomparison. All products qualitatively capture the macroscale spatial variability of observed SMB, but it is not possible to rank their relative performance because of the sparse observations at coastal regions with an elevation range from 200 to 1000 m. In terms of the absolute amount of observed snow accumulation in interior Antarctica, RACMO2.3 fits best, while the other models either underestimate (JRA-55, MERRA, ERA-Interim, and RACMO2.1) or overestimate (PMM5) the accumulation. Despite underestimated precipitation by the three reanalyses and RACMO2.1, this feature is clearly improved in JRA-55. However, because of changes in the observing system, especially the dramatically increased satellite observations for data assimilation, JRA-55 presents a marked jump in snow accumulation around 1979 and a large increase after the late 1990s. Although precipitation seasonality over the whole ice sheet is common for all products, ERA-Interim provides an unrealistic estimate of precipitation seasonality on the East Antarctic plateau, with high precipitation strongly peaking in summer. ERA-Interim shows a significant correlation with interannual variability of observed snow accumulation measurements at 28 of 29 locations, whereas fewer than 20 site observations significantly correlate with simulations by the other models. This suggests that ERA-Interim exhibits the highest performance of interannual variability in the observed precipitatio

The p53 Family of Transcription Factors Represses the Alpha- fetoprotein Gene Expression in Hepatocellular Carcinoma

Background: p53 deletion and mutation as well as upregulation of alpha-fetoprotein (AFP) are hallmarks of hepatocarcinogenesis. p63 and p73 belong to the family of p53-related transcription factors expressing a variety of isoforms. The expression of dominant negative (ΔN) p73 is related to the reduced survival of patients with hepatocellular carcinoma (HCC). In this study, we characterized the interaction between p53 family-dependent signaling pathways and the regulation of AFP at the gene and protein levels as essential determinants of therapeutic response and prognosis in HCC. Methods: Putative p53-, p63- and p73-binding sites within the AFP gene were identified in silico. Hep3B cells were transfected with plasmids encoding for p53, p63 and p73 to analyze the interplay of the p53 family with AFP. AFP transcription was determined by RT-qPCR. Protein levels of AFP, p53, p63 and p73 were analyzed by Western blot. Results: Underlining the importance of the crosstalk between the p53 family-dependent pathways and AFP regulation we identified eight novel putative binding sites for the members of the p53 family within the introns 1, 2, 3, 4, 7, 8, 11, and 12 of the AFP gene. Accordingly, full-length isoforms of p53, p63 and p73 efficiently downregulated AFP both on mRNA and protein level. Thus, the p53 family members were identified to be major regulators of AFP repression. Of note, p63 was characterized as a novel and p73 as the most efficient repressor of AFP. Conclusion: p53 mutation and upregulation of AFP are essential oncogenic events in the development of HCC. Here we show that AFP gene regulation occurs via a combined action of the p53 family members p53, p63 and p73. All three tumor suppressors reduce AFP gene and protein expression. Thus, our findings reveal a novel interaction of p53 family-dependent signaling pathways and AFP regulation at the gene and protein levels in HCC

Evaluating the impact of interprofessional training wards on patient satisfaction and clinical outcomes: a mixed-methods analysis

Introduction: Interprofessional teamwork is pivotal in modern healthcare, prompting the establishment of interprofessional training wards since 1996. While these wards serve as hubs for optimizing healthcare professional collaboration and communication, research into patient outcomes remains notably sparse and geographically limited, predominantly examining patient satisfaction and sparingly exploring other metrics like mortality or self-discharge rates. This study seeks to bridge this gap, comparing patient outcomes in interprofessional training wards and conventional wards under the hypothesis that the former offers no disadvantage to patient outcomes. Materials and methods: We explored patient outcomes within an interprofessional student ward called A-STAR at a University Hospital from October 2019 to December 2022. Engaging with patients discharged between May 2021 and April 2022, we utilized digital and paper-based anonymous questionnaires, catering to patient preference, to gather pertinent data. Results: Analysis of outcomes for 1,482 A-STAR (interprofessional student ward) and 5,752 conventional ward patients revealed noteworthy findings. A-STAR patients tended to be younger (59 vs. 61 years, p < 0.01) and more frequently male (73.5% vs. 70.4%, p = 0.025). Vital clinical outcomes, such as discharges against medical advice, complication-driven readmissions, and ICU transfers, were statistically similar between groups, as were mortality rates (1.2% vs. 1.3%, p = 0.468). A-STAR demonstrated high patient satisfaction, underscored by positive reflections on team competence, ward atmosphere, and responsiveness to concerns, emphasizing the value placed on interprofessional collaboration. Patient narratives commended team kindness, lucid explanations, and proactive involvement. Discussion: This data collectively underscores the safety and reliability of patient care within training wards, affirming that patients can trust the care provided in these settings. Patients on the interprofessional ward demonstrated high satisfaction levels: 96.7% appreciated the atmosphere and conduct of ward rounds. In comparison, 98.3% were satisfied with the discussion and information about their treatment during their hospital stay