Molecular and serological detection of Enterotoxigenic Staphylococcus aureus from traditionally dairy products

زمینه و هدف: استافیلوکوکوس اورئوس یکی از مهمترین عوامل مسمومیت در مواد غذایی و لبنی است. انتروتوکسین های استافیلوکوکی فاکتور اصلی ایجاد مسمومیت غذایی می باشد که از تیپ‌های مختلفی تشکیل شده‌اند. مهمترین آنها انتروتوکسین های تیپ A (SEA) و B (SEB) می باشد. هدف از این مطالعه تشخیص استافیلوکوکوس اورئوس تولید کننده انتروتوکسین تیپ A و B به روش مولکولی و سرولوژیک از مواد لبنی سنتی می باشد. روش بررسی: در این تحقیق توصیفی – تحلیلی آزمایشگاهی با رعایت شرایط استریل از 100 نمونه مواد لبنی تهیه شده به روش سنتی در سطح شهر تهران نمونه برداری و به آزمایشگاه منتقل شد. نمونه ها با استفاده از روش های متداول باکتری شناسی کشت داده شده و استافیلوکوکوس اورئوس ها شناسایی شدند. ژن های SEA و SEB در استافیلوکوکوس اورئوس های جدا شده، به روش PCR شناسایی شد. قدرت انتروتوکسین زایی سویه های دارای این ژن ها، به روش کشت در کیسه دیالیز بررسی شد و بوسیله آزمایش سرولوژیک ایمنودیفیوژن منفرد شعاعی (SRID) تایید گردید. داده ها با استفاده از آزمون آماری کای اسکوئر مورد تجزیه و تحلیل قرار گرفت. یافته ها: 32 از مواد غذایی از نظر وجود استافیلوکوکوس اورئوس مثبت بودند (خامه 18، پنیر 10 و شیر 4). نتایج آزمایش PCR نشان داد که از میان استافیلوکوک های جدا شده، 6/15 دارای ژن SEA، 3/9 دارای ژن SEB و 2/6 آنها دارای هردو ژن SEA و SEB هستند (05/0

RELATIONSHIP BETWEEN DIFFERENT ALLOGAMIC ASSOCIATED TRAIT CHARACTERISTICS OF THE FIVE NEWLY DEVELOPED CYTOPLASMIC MALE STERILE (CMS) LINES IN RICE

Five suitable maintainer varieties were identifi ed through testcrosses with IR58025A and the transfer of wild abortive cytoplasm was carried out by seven successive backcrosses. Five new CMS lines were developed by this approach in well adapted high yielding improved varietal background such as ‘Nemat’, ‘Neda’, ‘Dasht’, ‘Amol3’ and ‘Champa’. Agronomical characterization and allogamy-associated traits of the fi ve newly developed CMS lines were studied for their interrelationship. Anther length had a signifi cant positive correlation with the duration of glume opening (0.759) and high correlation of (0.698) with the angle between lemma and palea. The results indicated that ‘Nemat’ A, ‘Neda’ A, ‘Dasht’ A are more suitable as parents for hybrid seed production due to their favorable and superior fl oral characteristics in comparison to IR58025A

Investigating the Successful Experiences of Countries in Employing Nurse Practitioners to Provide Primary Health Care: Recommendations for Iran

Health systems are responsible for improving people’s health and protecting them against the financial costs of illness while respecting their dignity and status. In recent decades, the health systems of developing countries have put extensive reform programs on their agenda to meet the growing needs of patients

RELATIONSHIP BETWEEN DIFFERENT ALLOGAMIC ASSOCIATED TRAIT CHARACTERISTICS OF THE FIVE NEWLY DEVELOPED CYTOPLASMIC MALE STERILE (CMS) LINES IN RICE

Five suitable maintainer varieties were identifi ed through testcrosses with IR58025A and the transfer of wild abortive cytoplasm was carried out by seven successive backcrosses. Five new CMS lines were developed by this approach in well adapted high yielding improved varietal background such as ‘Nemat’, ‘Neda’, ‘Dasht’, ‘Amol3’ and ‘Champa’. Agronomical characterization and allogamy-associated traits of the fi ve newly developed CMS lines were studied for their interrelationship. Anther length had a signifi cant positive correlation with the duration of glume opening (0.759) and high correlation of (0.698) with the angle between lemma and palea. The results indicated that ‘Nemat’ A, ‘Neda’ A, ‘Dasht’ A are more suitable as parents for hybrid seed production due to their favorable and superior fl oral characteristics in comparison to IR58025A

Geometrically designed domain wall trap in tri-segmented nickel magnetic nanowires for spintronics devices

[EN] “Domain wall traps” have been engineered and well-exploited in nanostrips by creating a geometrical trapping site, e.g. a single notch along a stripe, compared to diameter-modulated (DM) cylindrical magnetic nanowires (NWs) where multi-segmented DM-NWs have been generally studied. Here, we report our systematic study on the magnetization behavior, domain wall structure and its nucleation/propagation in tri-segmented diameter-modulated Ni nanowires, a simple system to investigate the magnetization reversal as function of segment geometry and lay-out order. We find out that the magnetization behavior of single Ni DM-NWs exhibits the significance of positional ordering of thick and thin segments, distinguished by two distinct geometries including: dumbbell-type (type I) and rolling pin-type (type II). Based on experimental and theoretical simulations, it was evidenced that the wide-narrow junctions create trap sites for domain walls where the narrow segment restricts their motion. This type of geometrically engineered nanowires exhibit potential efficiency for future novel spintronic devices in particular when assembled in arrays of DM-NWs as a practical three-dimensional memory device.The financial support of Iranian National Science Foundation (INSF) under a grant number of 94010670 is acknowledged. F.N. acknowledges the financial support of CSIC to visit ICMM at Madrid. S.M.P. acknowledges the visiting scholarship to ICMM from Iranian Ministry of Science, Research and Technology and Sahand University research affair’s research studentship grant. Authors wish to thank Agustina Asenjo from ICMM/CSIC for her support in the MFM imaging and interpretation. This work has been partly supported by the Spanish Ministry of Economy, Industry and Competitiveness (MINECO) under project MAT2016-76824-C3-1-R and by the Regional Government of Madrid under project S2018/NMT-4321 NANOMAGCOST-CM. A.P. is co-financed by Greece and the European Union (European Social Fund ESF) through the Operational Programme «Human Resources Development, Education and Lifelong Learning» in the context of the project “Strengthening Human Resources Research Potential via Doctorate Research” (MIS-5000432), implemented by the State Scholarships Foundation (ΙΚΥ). DK acknowledges financial support from the Special Account for Research of ASPETE through project NANOSKY (No 80146)