The testament of Fernán Díaz de Toledo, El Relator (1455)

El objeto de este trabajo es dar a conocer el testamento de uno de los personajes fundamentales en el devenir histórico de los años centrales del s. XV en la corona de Castilla, el relator Fernán Díaz de Toledo, tal vez el hombre más poderoso en la corte de Juan II; testamento que, como se verá y es natural, nos proporciona un importante número de informaciones referentes a su familia.The aim of this paper is to edit the last will of Fernán Díaz de Toledo, who was probably the most powerful man at the court of King Juan II of Castille, and remains as a key person in mid-15th century castilian history. As it is usual in this kind of documents, Díaz de Toledo’s will sheds much light about his family, specially his converso origin

Dos nuevos documentos del monasterio de San Pedro de Villanueva (siglos XIV-XV)

The benedictine monastery of San Pedro de Villanueva, next to Cangas de Onís (Asturias) has suffered, as the other two great monasteries of the Asturian east -San Salvador de Celorio and San Antolín de Bedón- the disappearance of its medieval archive. In the present work are published and studied two recently recovered documents: a loan and a sale, whose special characteristics make them deserving of study

La ciencia de la Diplomática, desde sus orígenes hasta el mundo actual

Desde "De re diplomatica libri sex" de Jean Mabillon hasta los cercanos análisis de diplomática contemporánea, esta disciplina, enmarcada en el seno de las Ciencias y Técnicas Historiográficas, ha generado larga nómina de autores y estudiosos, retos y logros, que pretende glosar la presentación adjunta.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

Paleografía de la Baja Edad Media castellana

The history of the scripts used in the reign of the Crown of Castilla and Leon during the early Middle Ages lacks, till now, of meticulous studies related to different places, to chronology and to uses of wide general studies clearly structured. This essay would like, in the reduced space I have been allowed to dispose of, to point out the material shortages about this topic and to open new horizons, mainly based on the realization of partial studies and on the adjustment to the applicable terminology of the genuine types used during a wide period of time.L'histoire des écritures utilisées dans les règnes de la Couronne de Castille et Léon pendant le bas Moyen Age souffre, jusqu’à maintenant, d’un manque d'études minutieuses se rapportant à différents lieux, chronologies et usages et aussi d’études approfondies sur un ensemble clairement structuré. Cet article prétend, compte tenu du peu d'espace dont je disposais, faire ressortir les carences matérielles existantes sur le thème et ouvrir de nouvelles perspectives, basées fordamentalement sur la réalisation d’études partielles et sur l'adaptation de la terminologie applicable aux types authentiques utilisés pendant un si long laps de temps

Paleografía II. Las escrituras góticas desde 1250 hasta la imprenta.

Actas de las V Jornadas de la Sociedad Española de Ciencias y Técnicas Historiográficas (5ª, 2007, Oviedo

Salt influence on glutathione*/Schistosoma japonicum glutathione S-transferase binding

There has been some speculation about the salt independence of Schistosoma japonicum glutathione S-transferase (Sj26GST, EC. 2.5.1.18), but this aspect has not been carefully studied before. To establish the basis for a further development of this dependence, we have performed a methodical study of the influence of some important ions and their concentration on the binding properties of glutathione to Sj26GST by means of isothermal calorimetry and fluorescence quenching. Salts like NaCl, Na2SO4 and MgSO4 do not change practically the affinity of the protein for its substrate, whilst MgCl2 has the effect of decreasing the affinity as its concentration rises. However, the enthalpy change is not affected by all the salts studied, and so, the entropy change is the causal factor in dropping the affinity. We also looked at the conformational stability of the protein under different conditions to check the structural changes they provide, and found that the unfolding parameters are practically not affected by the salt concentration. We discuss the results in terms of the chaotropic nature of the ions implied