1993 Annual Report Nebraska Game and Parks Commisison

Table of ContentsAdministration ... 2Budget and Fiscal ... 3Engineering ... 7Fisheries ... 10Information and Education ... 12 Law Enforcement ... 15Operations and Construction ... 17Outdoor Education ... 19Parks ... 20Planning and Programming ... 22Realty ... 23Resource Services ... 24Wildlife ... 2

Monopolar electrocautery use in minimally invasive urosurgery: Case report of ectopic ureter management in a bitch

Background: Ureteral ectopy is a congenital anomaly, affecting young dogs, predominantly bitches. The main complication of the disease is urinary incontinence, which leads to low life quality for both animals and their owners. However, only two less invasive surgical management options are reported. Laser ablation is quite popular, while monopolar electrocautery use is very rare.Case Description: A 3-month and 1-week-old, 1.7 kg female Poodle was admitted to the Vetamicus clinic with severe urinary incontinence and moisture-associated dermatitis. After diagnosing intramural ectopy type during computed tomography scan and video cystoscopy, a search for surgical management options began. We proceeded with the monopolar cauterization technique, where a semirigid Karl Storz monopolar coagulating ball electrode is used to cut a mucous membrane partition between the ureter and urinary bladder. No complications occurred during or after the surgery. Ureter successfully healed and full continence was achieved together with sporadically using phenylpropanolamine syrup.Conclusion: The present case indicates that monopolar electrocautery use in intramural ectopy type management and possibly other urinary tract pathologies might be strongly beneficial

Molecular detection and genetic diversity of Babesia canis in ticks and pet dogs in Lithuania

Babesia canis, a haemoprotozoan parasite, is the main cause of a tick-borne infection of dogs-canine babesiosis. During the last decade, a spread of canine babesiosis due to B.canis to the previously non-endemic areas has been reported in Europe. Expansion of B.canis in Baltic countries is associated mainly with the expanding range of the main vector – Dermacentor reticulatu sticks and international travel. Genetic variability and antigenic variation are important mechanisms for the survival of Babesia parasites in their vertebrate hosts. Differences in the virulence of B.canis are associated with observed genetic heterogeneity among B.canis strains. We have investigated the prevalence of B.canis in D.reticulatus ticks along its current distribution range in Lithuania and the genetic diversity of B.canis strains isolated from ticks and naturally infected dogs. Partial regions of the 18S rRNA and Bc28.1 gene of B.canis were amplified through PCR assays, followed by PCR-RFLP and sequence analysis for molecular characterization of strains. B.canis was detected in 1.2% (26/2259) of D. reticulatus ticks with the prevalence varied in different locations from 0% to 11%. The molecular analysis indicates the presence of genetically heterogenic B.canis strains in Lithuania: three genotypes were detected based on 18S rRNR gene, while thirteen Bc28.1 gene sequence variants which divided into four genetically distinct groups were identified. Our findings on the genetic diversity of B. canis strains in Lithuania can be useful for the development of a polyvalent vaccine, assessing its potential for use and for predicting the risk of complicated diseasesBiologijos katedraVytauto Didžiojo universiteta

Successful management of acute kidney failure in a dog following calcipotriol ingestion

Objective: Calcipotriol intoxication in dogs is quite rare, but it can cause mineralization and necrosis of multiple organs including the kidneys [1]. Calcipotriol is highly toxic to dogs; the acute minimal toxic dose can be as low as 10 μg/kg and acute lethal dose is 65 μg/kg [2]. We report a case of toxic acute kidney failure after ingestion of calcipotriol in a dog, which was successfully treated with intermittent hemodialysis. Case report: A Bedlington terrier, 1-year-old, 9.8 kg chewed and ingested up to 30 g of Daivobet® ointment (containing calcipotriol 50 μg/g and betamethasone 0.5 mg/g). The ingested dose of calcipotriol was therefore up to 1500 μg (153 μg/kg). Emesis was inducted after approximately 2 hours, with a small amount of ointment found in the vomitus. The first symptoms manifested the next day with depression, anorexia, vomiting, and polyuria. The first blood analysis revealed hypercalcemia (3.54 mmol/L, reference 2.2–3.0) and hyperphosphatemia (3.43mmol/L, reference 0.8–2.2). The dog was treated with intravenous fluids, furosemide, and maropitant but its condition deteriorated. Blood analysis (urea 43.4 mmol/L [reference 3–10], creatinine 904 μmol/L [reference 30–140]) and urine specific gravity (1.008) revealed acute kidney failure on the 3rd day. The dog was transferred to a veterinary clinic with hemodialysis facilities and intermittent hemodialysis was started on the same day. Six hemodialysis sessions were performed on the 3rd, 4th, 5th, 6th, 9th, and 11th days. The first two of 0.5 and 1 hour duration were performed with 0.8m2 low-flux dialyzer, and the next four of 1.5 hours duration each, with 0.2m2 high-flux dialyzer. Mannitol infusion was used for disequilibrium syndrome prevention, and heparin for anticoagulation. Blood flow up to 70 mL/min (7 ml/kg/min) was reached without hemodynamic instability. The renal function started to improve from the 12th day and no f. [...]Lietuvos sveikatos mokslų universitetas. Medicinos akademijaSiaurio šnaucerisUAB "Siaurio šnauceris

Dirofilariasis in Lithuania

Dirofilaria infections in Europe are caused by Dirofilaria immitis and D.repens. Transmitted by mosquitoes, parasites can infect wild and domestic canines, felines, and humans. Climatic changes and an increase in the movement of reservoirs have an impact on the spread of dirofilariasis into previously non-endemic areas. In Lithuania, six human cases of D. repens infection were recorded during 2011-2015. In present study blood samples of 2180 randomly selected dogs presented in small animal clinic during 2014-2015 were investigated for filarial parasites. In four of dogs the adult worms were removed by using surgery technique. The microfilariae were determined on the basis of their morphometrical characteristics and the Diff-quick staining technique applied to blood smears and using Modified Knott’s test. For differentiation and accurate identification of the filarial species we also used PCR with panfilarial primers that amplify fragments of ITS2 region of rDNA from six different filariae species and species specific primers for amplification of COI gene of D. repens. Microfilariae were detected in blood smears of 4 (1.6%) dogs. Adults worm were identified as D. repens based on the macroscopic and microscopic characteristics and PCR analysis. Sequence analysis confirmed D. repens in all PCR positive samples. Additionally, blood samples collected from 101 shelter dogs were investigated for filarial parasites using PCR. D. repens was detected in 17.8% (18/101) of tested dogsBiologijos katedraGamtos mokslų fakultetasSiaurio šnaucerisVytauto Didžiojo universiteta

Molecular identification of new zoonotic parasites Dirofilaria repens in Lithuania

Biologijos katedraGamtos mokslų fakultetasVytauto Didžiojo universiteta

The first cases of Dirofilaria repens infection in dogs in Lithuania

Biologijos katedraGamtos mokslų fakultetasLietuvos sveikatos mokslų universitetas. Veterinarijos akademijaUAB Siaurio šnaucerisVytauto Didžiojo universiteta

Prevalence of Dirofilaria repens in dogs from Lithuania

Canine dirofilariasis cased by Dirofilaria repens and D. immitis during the past decade has become recognized worldwide as an emerging parasitic disease. The climate change, extensive movement of dogs across countries and continents has contributed to the expanding of distribution range of D. repens to the areas they had never been found before. In this study blood samples of 2180 randomly selected dogs presented in small animal clinic during 2014-2015 were investigated for filarial parasites. The microfilariae were determined on the basis of their morphometrical characteristics and the Diff-quick staining technique applied to blood smears and using Modified Knott’s test. For accurate identification of the filarial species we also used PCR with pan-filarial primers that amplify fragments of ITS-2 region of rDNA from six different filariae species and species specific primers for amplification of COI gene of D. repens. Microfilariae were detected in blood smears of 42 (1.9%) dogs. In four of dogs the adult worms were removed by using surgery technique. Adults worm were identified as D. repens based on the morphological and morphometric characteristics and PCR analysis. Sequence analysis confirmed D. repens in all PCR positive samples. Additionally, blood samples collected from 101 shelter dogs were investigated for filarial parasites using PCR. D. repens was detected in 18.9% (19/101) of tested dogsBiologijos katedraGamtos mokslų fakultetasVytauto Didžiojo universiteta

Prevalence of zoonotic mosquito-borne and tick-borne pathogens in domestic dogs from Lithuania

The incidence of vector-borne diseases has been reported to have increased worldwide in recent years, seriously threatening human and animal health. During the past decade, vector-borne diseases have been continuously spreading in Baltic countries including Lithuania. Information on canine vector-borne disease (CVBD) agents at the local and regional levels allows veterinarians to better recognize the pathogens that can affect dogs, thus facilitating diagnosis and treatment. Dogs could be infected with a large number of different CVBD agents such as Anaplasma phagocytophilum, Borrelia spp. and Dirofilaria spp. The filarioid nematodes Dirofilaria transmitted by the bite of infected mosquitoes have zoonotic significance, therefore accurate identification of Dirofilaria species in dogs is clinically important. Tick-borne diseases have great medical importance worldwide and affect dogs health through the transmission of pathogens by blood sucking Ixodidae ticks. The geographic distribution of infected ticks has expanded because of bird migration, environmental and climatic changes. Infection with A. phagocytophilum and Borrelia spp. in dogs is mostly asymptomatic or characterized by nonspecific clinical signs, therefore especially important to use appropriate methods for early diagnosis of pathogens. The aim of the present study was to investigate the presence of mosquito and tick-borne pathogens in domestic dogs using molecular DNA analysis methods. In total 193 blood samples were collected from domestic dogs in Lithuania during 2016-2018. DNA was isolated from EDTA-anticoagulated whole blood. Partial internal transcribed spacer region 2 (ITS2) of the ribosomal DNA and cytochrome c oxidase subunit I (COI) gene were used as targets in PCR for detection of canine microfilariae. For detection of A. phagocytophilum and Borrelia spp. DNA was used real-time PCR.[...]Biologijos katedraGamtos mokslų fakultetasVytauto Didžiojo universiteta

Uodų ir erkių platinamų patogenų molekulinis identifikavimas naminiuose šunyse

Uodai ir erkės yra pagrindiniai platinamų infekcinių ligų vektoriai visame pasaulyje. Nustatyta, kad dėl klimato šiltėjimo, besikeičiančių aplinkos sąlygų, gyvūnų populiacijos dinamikos ir vektorių judėjimo infekcinės ligos sparčiai plinta naujose geografinėse teritorijose. Dirofiliariozė yra zoonozinė liga, kuria gali užsikrėsti tiek gyvūnai, tiek žmonės nuo kraujasiurbių uodų platinamų nematodų [1]. Pastebėtas šios ligos protrūkis Europos šiaurinėje dalyje. Registruojami atvejai šalyse, kurios anksčiau laikytos ne epideminėmis: Austrija, Lenkija, Vokietija, Čekijos Respublika, Baltarusija, Latvija, Estija, taip pat Lietuva. Dirofiliariozė yra dviejų formų: širdies, kurią sukelia Dirofilaria immitis lervos, ir poodinė dirofiliariozė, kurią sukelia Dirofilaria repens [2]. Veterinarijos klinikose dėl nespecifinių ligos simptomų dirofiliariozės atvejai nustatomi atsitiktinai, atliekant laboratorinius tyrimus, todėl ypač svarbūs molekuliniai patogenų tyrimai, kurie leidžia aptikti ligos sukėlėjus besimptominiuose šunyse ir tiksliai identifikuoti sukėlėjų rūšį.[...]Biologijos katedraGamtos mokslų fakultetasVytauto Didžiojo universiteta