chronic urticaria is usually associated with fibromyalgia syndrome

Although the pathophysiology of chronic urticaria is not fully understood, it is possible that dysfunctioning of peripheral cutaneous nerve fibres may be involved. It has also been suggested that fibromyalgia syndrome, a multi-symptomatic chronic pain condition, may be associated with alterations and dysfunctioning of peripheral cutaneous nerve fibres. The aim of this study was to determine whether patients with chronic urticaria are also affected by fibromyalgia syndrome. A total of 126 patients with chronic urticaria were investigated for fibromyalgia syndrome. An unexpectedly high proportion (over 70%) had fibromyalgia syndrome. The corresponding proportion for 50 control dermatological patients was 16%, which is higher than previously published data for the Italian general population (2.2%). It is possible that dysfunctional cutaneous nerve fibres of patients with fibromyalgia syndrome may release neuropeptides, which, in turn, may induce dermal microvessel dilatation and plasma extravasation. Furthermore, some neuropeptides may favour mast cell degranulation, which stimulates nerve endings, thus providing positive feedback. Chronic urticaria may thus be viewed in many patients, as a consequence of fibromyalgia syndrome. Speculatively, skin neuropathy (fibromyalgia syndrome) may trigger neurogenic skin inflammation (chronic urticaria)

Motor Learning in Response to Different Experimental Pain Models Among Healthy Individuals: A Systematic Review

Learning new movement patterns is a normal part of daily life, but of critical importance in both sport and rehabilitation. A major question is how different sensory signals are integrated together to give rise to motor adaptation and learning. More specifically, there is growing evidence that pain can give rise to alterations in the learning process. Despite a number of studies investigating the role of pain on the learning process, there is still no systematic review to summarize and critically assess investigations regarding this topic in the literature. Here in this systematic review, we summarize and critically evaluate studies that examined the influence of experimental pain on motor learning. Seventeen studies that exclusively assessed the effect of experimental pain models on motor learning among healthy human individuals were included for this systematic review, carried out based on the preferred reporting items for systematic reviews and meta-analyses (PRISMA) statement. The results of the review revealed there is no consensus regarding the effect of pain on the skill learning acquisition and retention. However, several studies demonstrated that participants who experienced pain continued to express a changed motor strategy to perform a motor task even 1 week after training under the pain condition. The results highlight a need for further studies in this area of research, and specifically to investigate whether pain has different effects on motor learning depending on the type of motor task

Ten kilodalton heat shock protein (HSP10) is overexpressed during carcinogenesis of large bowel and uterine exocervix

In the present study, we evaluated the presence and the level of expression of HSP10 in two carcinogenetic models: the 'adenoma-carcinoma sequence' of large bowel and the 'dysplasia-carcinoma sequence' of uterine exocervix. We found HSP10 was overexpressed during the carcinogenesis of both organs. In particular, HSP10 was overexpressed early in large bowel carcinogenesis, while the expression of this protein in exocervical carcinogenesis gradually increased from normal through dysplastic to neoplastic tissues. The quantitative analysis of immunohistochemistry and the Western blotting confirmed these results. Our previous observations showed overexpression of HSP60 in the same carcinogenetic models. This report correlates the overexpression of HSP10 with that of HSP60 during carcinogenesis in vivo. These results could stimulate further studies on the pathogenetic role of these proteins during the carcinogenesis as well as their use as diagnostic and prognostic tools in oncology. \ua9 2003 Elsevier Science Ltd. All rights reserved

Evaluating meteorological climate model inputs to improve coastal hydrodynamic studies

Abstract. This work compares meteorological results from different regional climate model (RCM) implementations in the Mediterranean area, with a focus on the northern Adriatic Sea. The need to use these datasets as atmospheric forcings (wind and atmospheric pressure fields) for coastal hydrodynamic models to assess future changes in the coastal hydrodynamics, is the basis of the presented analysis. It would allow the assessment of uncertainties due to atmospheric forcings in providing coastal current, surge and wave climate changes from future implementations of hydrodynamic models. Two regional climate models, with different spatial resolutions, downscaled from two different global climate models (whose atmospheric components are, respectively, ECHAM4 and ECHAM5), were considered. In particular, the RCM delivered wind and atmospheric pressure fields were compared with measurements at four stations along the Italian Adriatic coast. The analyses were conducted using a past control period, 1960–1990, and the A1B IPCC future scenario (2070–2100). The chosen scenario corresponds to a world of very rapid economic and demographic growth that peaks in mid-century, with a rapid introduction of new efficient technologies, which balance fossil and non-fossil resources (IPCC, 2007). Consideration is given to the accuracy of each model at reproducing the basic statistics and the trends. The role of models' spatial resolution in reproducing global and local scale meteorological processes is also discussed. The Adriatic Sea climate is affected by the orography that produces a strengthening of north-eastern katabatic winds like bora. Therefore, spatial model resolution, both for orography and for a better resolution of coastline (Cavaleri et al., 2010), is one of the important factors in providing more realistic wind forcings for future hydrodynamic models implementations. However, also the characteristics in RCM setup and parameterization can explain differences between the datasets. The analysis from an ensemble of model implementation would provide more robust indications on climatic wind and atmospheric pressure variations. The scenario-control comparison shows a general increase in the mean atmospheric pressure values while a decrease in mean wind speed and in extreme wind events is seen, particularly for the datasets with higher spatial resolution

RUOLO DELL’ESERCIZIO FISICO NELLA QUALITÀ DI VITA DI SOGGETTI CON SCLEROSI MULTIPLA. ROLE OF EXERCISE IN THE QUALITY OF LIFE IN SUBJECTS WITH MULTIPLE SCLEROSIS.

La sclerosi multipla è una patologia neurodegenerativa cronica che ha un impatto considerevole sullo stato fisico, psicologico e sociale dei pazienti, influenzando negativamente la loro qualità di vita. Tra i fattori contribuenti ad una ridotta qualità di vita sono inclusi la presenza di dolore, spasmi, sindromi depressive, l’aumento della fatica e la riduzione della forza muscolare, mobilità ed autonomia nella deambulazione. Accanto alla medicina convenzionale, varie forme di medicina complementare ed alternativa, tra cui l’esercizio fisico, sono utilizzate dai soggetti con sclerosi multipla. Tuttavia, poche sono le prove scientifiche a sostegno della loro efficacia nel ridurre i sintomi derivanti dalla patologia e nel migliorare la loro qualità di vita. L’obiettivo del presente studio è stato quello di illustrare i risultati presenti nella letteratura scientifica riguardo l’utilizzo dell’esercizio fisico quale metodo sicuro ed efficace per migliorare la qualità di vita di soggetti con sclerosi multipla. Nel dettaglio, sono stati presi in considerazione studi che hanno valutato gli effetti dell’esercizio in acqua, dell’allenamento aerobico e degli esercizi di forza e di potenziamento muscolare sull’efficienza cardio-respiratoria, deambulazione e riduzione dei sintomi e della fatica in questi soggetti. In questi studi, i pazienti che hanno svolto programmi di esercizio fisico, hanno mostrato miglioramenti della forma fisica ed in particolare della forza muscolare, mobilità, equilibrio, postura e deambulazione. Inoltre, hanno riportato una riduzione significativa del dolore, della stanchezza, della spasticità, degli spasmi e della depressione. In alcuni casi, questi cambiamenti sono stati mantenuti anche dopo l’interruzione dell’attività fisica. Gli effetti della pressione idrostatica, durante l’esercizio in acqua ha portato, in generale, ad ottimi risultati a livello cardiovascolare e respiratorio. Infatti, l’esercizio fisico ha aumentato il fitness cardiovascolare dei soggetti con SM, come dimostrato dagli incrementi del picco di VO2max permettendo loro di svolgere in maniera più energica le attività quotidiane e ricreative, senza affanno. Dagli studi presi in esame è anche emerso che l’esercizio fisico influenza la sfera psicologica ed emotiva di questi pazienti aumentando l’autostima ed il tono dell’umore. Questo lavoro dimostra l’efficacia clinica dell’attività motoria sia dal punto di vista emotivo che della performance motoria proponendo attività gradite e coinvolgenti che soddisfano i pazienti stessi.Multiple sclerosis is a neurodegenerative disease that has a significant impact on the physical, psychological and social situation of patients, adversely affecting their quality of life. Among the factors contributing to a reduced quality of life are included the presence of pain, spasms, depressive syndromes, increased fatigue and reduced muscle strength, mobility and autonomy in walking. In addition to conventional medicine, various forms of complementary and alternative medicine, including exercise, are used by people with multiple sclerosis. However, there is little scientific evidence to support their effectiveness in reducing the symptoms caused by the disease and improving their quality of life. The aim of this study was to illustrate the results from the scientific literature regarding the use of physical exercise as a safe and effective method for improving the quality of life in subjects with multiple sclerosis. In detail, we have been taken into account studies that have evaluated the effects of aquatic, aerobic and strength exercises on cardio-respiratory efficiency, gait and reduced symptoms and fatigue in these subjects. In these studies, patients who performed exercise programs showed improvements in physical form and, in particular, in muscle strength, mobility, balance, posture and gait. In addition, they reported a significant reduction in pain, fatigue, spasticity, spasms and depression. In several cases, these changes were maintained after cessation of exercise. The effects of hydrostatic pressure, during the exercise in water has led, in general, to excellent results in the cardiovascular and respiratory systems. In fact, exercise has increased the cardiovascular fitness of people with MS, as demonstrated by increases in peak VO2max allowing them to perform daily and recreational activities in a more energetic way. The studies examined also showed that exercise affects the psychological and emotional aspect of these patients by increasing self-esteem and mood. This work demonstrates the clinical efficacy of physical activity both in terms of emotional and motor performance offering appreciated and engaging activities that satisfy the patients themselves

Effective strategies for promoting physical activity through the use of digital media among school-age children: A systematic review

Digital media are widespread among school-age children, and their incorrect use may lead to an increase in sedentary levels and the consequences associated with it. There are still few studies that have investigated whether physical activity levels could be increased through their use. The aim of this study was to systematically review the scientific literature in order to identify whether digital strategies and technologies are capable of increasing the level of physical activity. A literature search was performed using the following databases: Pubmed, Scopus, and Web of Science. The main outcomes evaluated the increase in physical activity levels, the number of steps, and the reduction of sedentary behaviors. Two trained researchers independently assessed eligible studies against eligibility criteria, extracted data, and assessed the risk of bias. The Downs and Black checklist was used to assess the quality of the included studies. A total of 15 studies (1122 children) were included in this systematic review, with a mean age of 8.45 ± 0.70 years. Quality assessment of the studies observed a “moderate quality” of the included records. The results of this systematic review highlight that digital media can be applied as a way to improve the levels of physical activity in children to contrast a sedentary lifestyle. The main limitations of the study are the heterogeneity within the exercise protocols and the paucity of studies involving school-age children. More research is needed to confirm our findings also due to continuing technological progress

The secreted triose phosphate isomerase of Brugia malayi is required to sustain microfilaria production in vivo

Human lymphatic filariasis is a major tropical disease transmitted through mosquito vectors which take up microfilarial larvae from the blood of infected subjects. Microfilariae are produced by long-lived adult parasites, which also release a suite of excretory-secretory products that have recently been subject to in-depth proteomic analysis. Surprisingly, the most abundant secreted protein of adult Brugia malayi is triose phosphate isomerase (TPI), a glycolytic enzyme usually associated with the cytosol. We now show that while TPI is a prominent target of the antibody response to infection, there is little antibody-mediated inhibition of catalytic activity by polyclonal sera. We generated a panel of twenty-three anti-TPI monoclonal antibodies and found only two were able to block TPI enzymatic activity. Immunisation of jirds with B. malayi TPI, or mice with the homologous protein from the rodent filaria Litomosoides sigmodontis, failed to induce neutralising antibodies or protective immunity. In contrast, passive transfer of neutralising monoclonal antibody to mice prior to implantation with adult B. malayi resulted in 60–70% reductions in microfilarial levels in vivo and both oocyte and microfilarial production by individual adult females. The loss of fecundity was accompanied by reduced IFNγ expression by CD4+ T cells and a higher proportion of macrophages at the site of infection. Thus, enzymatically active TPI plays an important role in the transmission cycle of B. malayi filarial parasites and is identified as a potential target for immunological and pharmacological intervention against filarial infections

Impaired photosystem I oxidation induces STN7-dependent phosphorylation of the light-harvesting complex I protein Lhca4 in Arabidopsis thaliana

Reduction of the plastoquinone (PQ) pool is known to activate phosphorylation of thylakoid proteins. In the Arabidopsis thaliana mutants psad1-1 and psae1-3, oxidation of photosystem I (PSI) is impaired, and the PQ pool is correspondingly over-reduced. We show here that, under these conditions, the antenna protein Lhca4 of PSI becomes a target for phosphorylation. Phosphorylation of the mature Lhca4 protein at Thr16 is suppressed in stn7 psad1 and stn7 psae1 double mutants. Thus, under extreme redox conditions, hyperactivation of thylakoid protein kinases and/or reorganization of thylakoid protein complex distribution increase the susceptibility of PSI to phosphorylation

In Vitro Uptake of 140 kDa Bacillus thuringiensis Nematicidal Crystal Proteins by the Second Stage Juvenile of Meloidogyne hapla

Plant-parasitic nematodes (PPNs) are piercing/sucking pests, which cause severe damage to crops worldwide, and are difficult to control. The cyst and root-knot nematodes (RKN) are sedentary endoparasites that develop specialized multinucleate feeding structures from the plant cells called syncytia or giant cells respectively. Within these structures the nematodes produce feeding tubes, which act as molecular sieves with exclusion limits. For example, Heterodera schachtii is reportedly unable to ingest proteins larger than 28 kDa. However, it is unknown yet what is the molecular exclusion limit of the Meloidogyne hapla. Several types of Bacillus thuringiensis crystal proteins showed toxicity to M. hapla. To monitor the entry pathway of crystal proteins into M. hapla, second-stage juveniles (J2) were treated with NHS-rhodamine labeled nematicidal crystal proteins (Cry55Aa, Cry6Aa, and Cry5Ba). Confocal microscopic observation showed that these crystal proteins were initially detected in the stylet and esophageal lumen, and subsequently in the gut. Western blot analysis revealed that these crystal proteins were modified to different molecular sizes after being ingested. The uptake efficiency of the crystal proteins by the M. hapla J2 decreased with increasing of protein molecular mass, based on enzyme-linked immunosorbent assay analysis. Our discovery revealed 140 kDa nematicidal crystal proteins entered M. hapla J2 via the stylet, and it has important implications in designing a transgenic resistance approach to control RKN