Термічний аналіз деревини та кори робінії несправжньоакації в деревостанах Північного степу України

The thermal degradation of the wood and bark of the main forest-forming spesies of artificial forest plantations of the Northern Steppe of Ukraine – Black locust (Robinia pseudoacacia L.) has been investigated using thermogravimetric methods – a thermogravimetric curve (TG) and a differential thermogravimetric curve (DTG), or a curve of the rate of change in the mass of the investigated sample. Thermal analysis of samples of wood and bark was carried out in an oxidizing (air) atmosphere. Some stages of thermal decomposition of wood and bark under conditions of a programmable heating up to 600°C at heating rates 10°C/min (TG/DTG/DTA) and their temperature intervals, mass loss, mass loss intensity and thermal effects have also been investigated. Wood and bark samples were fully characterized from that data which deals with the activation energy analysis at the individual stages of thermal decomposition. The dependences of the activation energy on the degree of conversion, as well as by comparison of the mass loss at the appropriate stages of the thermal degradation, thermal effects, residual mass and some other parameters of TG/DTG have also been evaluated. A greater content of constitutional moisture in the composition of the bark samples with decrease in the temperature of the onset of intensive destruction of the bark in comparison with wood have been revealed. The results of thermogravimetric measurements were analysed in the framework of the kinetic model of Brodo. The activation energy of processes of bark and wood destruction in each temperature range and in the whole investigated range is calculated and mathematical models are proposed for estimating the dependence of mass loss on the temperature degradation of the components of the aboveground phytomass of the studied forest-forming species. Comparison of DTA curves showed that the thermal effect of thermooxidative destruction of the bark as a whole is greater than that of wood, which correlates well with a decrease in the activation energy.За допомогою методів термогравіметрії – термогравіметричної кривої (ТГ), диференціальної термогравіметричної кривої (ДТГ), або кривої інтенсивності зміни маси досліджуваного зразка, досліджено термічну деструкцію деревини і кори головної лісотвірної породи штучних лісових насаджень Північного Степу України – робінії несправжньоакації (Robinia pseudoacacia L.). Термічний аналіз зразків деревини і кори здійснено в окиснювальній (повітря) атмосфері. Встановлено стадії термічного розкладання деревинної речовини і кори в умовах програмованого нагріву до 600 оС зі швидкістю 10 оС/хв (ТГ/ДТГ/ДТА), їх температурні інтервали, втрату маси, інтенсивність втрати маси та теплові ефекти. На основі аналізу величин енергії активації на окремих стадіях термічного розкладання, залежності енергії активації від ступеня конверсії деревини і кори, а також із порівняння втрати маси на відповідних стадіях термодеструкції, теплових ефектів, залишкової маси і інших параметрів ТГ/ДТГ, охарактеризовано деревину і кору робінії. Деревина робінії характеризується значнішою термостабільністю, ніж кора. Запропоновано математичні моделі для оцінювання залежності втрати маси від температури деструкції складників надземної фітомаси (кори, деревини) досліджуваного деревного виду

Baryogenesis vs. proton stability in theories with extra dimensions

We propose a new scenario of baryogenesis in the context of theories with large extra dimensions. The baryon number is almost conserved at zero temperature by means of a localization mechanism recently analyzed by Arkani-Hamed and Schmaltz: leptons and quarks are located at two slightly displaced positions in the extra space, and this naturally suppresses the interactions which ``convert'' the latter in the former. We show that this is expected to be no longer true when finite temperature effects are taken into account. The whole scenario is first presented in its generality, without referring to the bulk geometry or to the specific mechanism which may generate the baryon asymmetry. As an example, we then focus on a baryogenesis model reminiscent of GUT baryogenesis. The Sakharov out of equilibrium condition is satisfied by assuming nonthermal production of the bosons that induce baryon number violation.Comment: 10 pages, 1 figur

Steering hyper-giants' traffic at scale

Large content providers, known as hyper-giants, are responsible for sending the majority of the content traffic to consumers. These hyper-giants operate highly distributed infrastructures to cope with the ever-increasing demand for online content. To achieve 40 commercial-grade performance of Web applications, enhanced end-user experience, improved reliability, and scaled network capacity, hyper-giants are increasingly interconnecting with eyeball networks at multiple locations. This poses new challenges for both (1) the eyeball networks having to perform complex inbound traffic engineering, and (2) hyper-giants having to map end-user requests to appropriate servers. We report on our multi-year experience in designing, building, rolling-out, and operating the first-ever large scale system, the Flow Director, which enables automated cooperation between one of the largest eyeball networks and a leading hyper-giant. We use empirical data collected at the eyeball network to evaluate its impact over two years of operation. We find very high compliance of the hyper-giant to the Flow Director’s recommendations, resulting in (1) close to optimal user-server mapping, and (2) 15% reduction of the hyper-giant’s traffic overhead on the ISP’s long-haul links, i.e., benefits for both parties and end-users alike.EC/H2020/679158/EU/Resolving the Tussle in the Internet: Mapping, Architecture, and Policy Making/ResolutioNe

Disruption of Higher Order DNA Structures in Friedreich's Ataxia (GAA)n Repeats by PNA or LNA Targeting

Expansion of (GAA)n repeats in the first intron of the Frataxin gene is associated with reduced mRNA and protein levels and the development of Friedreich’s ataxia. (GAA)n expansions form non-canonical structures, including intramolecular triplex (H-DNA), and R-loops and are associated with epigenetic modifications. With the aim of interfering with higher order H-DNA (like) DNA structures within pathological (GAA)n expansions, we examined sequence-specific interaction of peptide nucleic acid (PNA) with (GAA)n repeats of different lengths (short: n=9, medium: n=75 or long: n=115) by chemical probing of triple helical and single stranded regions. We found that a triplex structure (H-DNA) forms at GAA repeats of different lengths; however, single stranded regions were not detected within the medium size pathological repeat, suggesting the presence of a more complex structure. Furthermore, (GAA)4-PNA binding of the repeat abolished all detectable triplex DNA structures, whereas (CTT)5-PNA did not. We present evidence that (GAA)4-PNA can invade the DNA at the repeat region by binding the DNA CTT strand, thereby preventing non-canonical-DNA formation, and that triplex invasion complexes by (CTT)5-PNA form at the GAA repeats. Locked nucleic acid (LNA) oligonucleotides also inhibited triplex formation at GAA repeat expansions, and atomic force microscopy analysis showed significant relaxation of plasmid morphology in the presence of GAA-LNA. Thus, by inhibiting disease related higher order DNA structures in the Frataxin gene, such PNA and LNA oligomers may have potential for discovery of drugs aiming at recovering Frataxin expression

Global Retinoblastoma Presentation and Analysis by National Income Level.

Importance: Early diagnosis of retinoblastoma, the most common intraocular cancer, can save both a child's life and vision. However, anecdotal evidence suggests that many children across the world are diagnosed late. To our knowledge, the clinical presentation of retinoblastoma has never been assessed on a global scale. Objectives: To report the retinoblastoma stage at diagnosis in patients across the world during a single year, to investigate associations between clinical variables and national income level, and to investigate risk factors for advanced disease at diagnosis. Design, Setting, and Participants: A total of 278 retinoblastoma treatment centers were recruited from June 2017 through December 2018 to participate in a cross-sectional analysis of treatment-naive patients with retinoblastoma who were diagnosed in 2017. Main Outcomes and Measures: Age at presentation, proportion of familial history of retinoblastoma, and tumor stage and metastasis. Results: The cohort included 4351 new patients from 153 countries; the median age at diagnosis was 30.5 (interquartile range, 18.3-45.9) months, and 1976 patients (45.4%) were female. Most patients (n = 3685 [84.7%]) were from low- and middle-income countries (LMICs). Globally, the most common indication for referral was leukocoria (n = 2638 [62.8%]), followed by strabismus (n = 429 [10.2%]) and proptosis (n = 309 [7.4%]). Patients from high-income countries (HICs) were diagnosed at a median age of 14.1 months, with 656 of 666 (98.5%) patients having intraocular retinoblastoma and 2 (0.3%) having metastasis. Patients from low-income countries were diagnosed at a median age of 30.5 months, with 256 of 521 (49.1%) having extraocular retinoblastoma and 94 of 498 (18.9%) having metastasis. Lower national income level was associated with older presentation age, higher proportion of locally advanced disease and distant metastasis, and smaller proportion of familial history of retinoblastoma. Advanced disease at diagnosis was more common in LMICs even after adjusting for age (odds ratio for low-income countries vs upper-middle-income countries and HICs, 17.92 [95% CI, 12.94-24.80], and for lower-middle-income countries vs upper-middle-income countries and HICs, 5.74 [95% CI, 4.30-7.68]). Conclusions and Relevance: This study is estimated to have included more than half of all new retinoblastoma cases worldwide in 2017. Children from LMICs, where the main global retinoblastoma burden lies, presented at an older age with more advanced disease and demonstrated a smaller proportion of familial history of retinoblastoma, likely because many do not reach a childbearing age. Given that retinoblastoma is curable, these data are concerning and mandate intervention at national and international levels. Further studies are needed to investigate factors, other than age at presentation, that may be associated with advanced disease in LMICs

The global retinoblastoma outcome study : a prospective, cluster-based analysis of 4064 patients from 149 countries

DATA SHARING : The study data will become available online once all analyses are complete.BACKGROUND : Retinoblastoma is the most common intraocular cancer worldwide. There is some evidence to suggest that major differences exist in treatment outcomes for children with retinoblastoma from different regions, but these differences have not been assessed on a global scale. We aimed to report 3-year outcomes for children with retinoblastoma globally and to investigate factors associated with survival. METHODS : We did a prospective cluster-based analysis of treatment-naive patients with retinoblastoma who were diagnosed between Jan 1, 2017, and Dec 31, 2017, then treated and followed up for 3 years. Patients were recruited from 260 specialised treatment centres worldwide. Data were obtained from participating centres on primary and additional treatments, duration of follow-up, metastasis, eye globe salvage, and survival outcome. We analysed time to death and time to enucleation with Cox regression models. FINDINGS : The cohort included 4064 children from 149 countries. The median age at diagnosis was 23·2 months (IQR 11·0–36·5). Extraocular tumour spread (cT4 of the cTNMH classification) at diagnosis was reported in five (0·8%) of 636 children from high-income countries, 55 (5·4%) of 1027 children from upper-middle-income countries, 342 (19·7%) of 1738 children from lower-middle-income countries, and 196 (42·9%) of 457 children from low-income countries. Enucleation surgery was available for all children and intravenous chemotherapy was available for 4014 (98·8%) of 4064 children. The 3-year survival rate was 99·5% (95% CI 98·8–100·0) for children from high-income countries, 91·2% (89·5–93·0) for children from upper-middle-income countries, 80·3% (78·3–82·3) for children from lower-middle-income countries, and 57·3% (52·1-63·0) for children from low-income countries. On analysis, independent factors for worse survival were residence in low-income countries compared to high-income countries (hazard ratio 16·67; 95% CI 4·76–50·00), cT4 advanced tumour compared to cT1 (8·98; 4·44–18·18), and older age at diagnosis in children up to 3 years (1·38 per year; 1·23–1·56). For children aged 3–7 years, the mortality risk decreased slightly (p=0·0104 for the change in slope). INTERPRETATION : This study, estimated to include approximately half of all new retinoblastoma cases worldwide in 2017, shows profound inequity in survival of children depending on the national income level of their country of residence. In high-income countries, death from retinoblastoma is rare, whereas in low-income countries estimated 3-year survival is just over 50%. Although essential treatments are available in nearly all countries, early diagnosis and treatment in low-income countries are key to improving survival outcomes.The Queen Elizabeth Diamond Jubilee Trust and the Wellcome Trust.https://www.thelancet.com/journals/langlo/homeam2023Paediatrics and Child Healt