38 research outputs found
A possible juvenile hypochondroplasia case from the mass grave of Lazzaretto Nuovo Island (Venice)
Among the remains of individuals buried in the cemetery of the New Lazaretto (Venice) during the plague epidemic of 1576, a juvenile skeleton with a discrepancy between the biological age at death obtained by the diaphyseal length was recovered. Other skeletal indicators from the humerus and the shoulder girdle show a craniocaudal reduction of bone length. Associated with other morphological changes and signs, the individual is diagnosed with hypochondroplasia, a specific form of dwarfism
Two-omics data revealed commonalities and differences between Rpv12- and Rpv3-mediated resistance in grapevine
Plasmopara viticola is the causal agent of grapevine downy mildew (DM). DM resistant varieties deploy effector-triggered immunity (ETI) to inhibit pathogen growth, which is activated by major resistance loci, the most common of which are Rpv3 and Rpv12. We previously showed that a quick metabolome response lies behind the ETI conferred by Rpv3 TIR-NB-LRR genes. Here we used a grape variety operating Rpv12-mediated ETI, which is conferred by an independent locus containing CC-NB-LRR genes, to investigate the defence response using GC/MS, UPLC, UHPLC and RNA-Seq analyses. Eighty-eight metabolites showed significantly different concentration and 432 genes showed differential expression between inoculated resistant leaves and controls. Most metabolite changes in sugars, fatty acids and phenols were similar in timing and direction to those observed in Rpv3-mediated ETI but some of them were stronger or more persistent. Activators, elicitors and signal transducers for the formation of reactive oxygen species were early observed in samples undergoing Rpv12-mediated ETI and were paralleled and followed by the upregulation of genes belonging to ontology categories associated with salicylic acid signalling, signal transduction, WRKY transcription factors and synthesis of PR-1, PR-2, PR-5 pathogenesis-related proteins
Algebraic Comparison of Partial Lists in Bioinformatics
The outcome of a functional genomics pipeline is usually a partial list of
genomic features, ranked by their relevance in modelling biological phenotype
in terms of a classification or regression model. Due to resampling protocols
or just within a meta-analysis comparison, instead of one list it is often the
case that sets of alternative feature lists (possibly of different lengths) are
obtained. Here we introduce a method, based on the algebraic theory of
symmetric groups, for studying the variability between lists ("list stability")
in the case of lists of unequal length. We provide algorithms evaluating
stability for lists embedded in the full feature set or just limited to the
features occurring in the partial lists. The method is demonstrated first on
synthetic data in a gene filtering task and then for finding gene profiles on a
recent prostate cancer dataset
A new classification method using array Comparative Genome Hybridization data, based on the concept of Limited Jumping Emerging Patterns
<p>Abstract</p> <p>Background</p> <p>Classification using aCGH data is an important and insufficiently investigated problem in bioinformatics. In this paper we propose a new classification method of DNA copy number data based on the concept of limited Jumping Emerging Patterns. We present the comparison of our limJEPClassifier to SVM which is considered the most successful classifier in the case of high-throughput data.</p> <p>Results</p> <p>Our results revealed that the classification performance using limJEPClassifier is significantly higher than other methods. Furthermore, we show that application of the limited JEP's can significantly improve classification, when strongly unbalanced data are given.</p> <p>Conclusion</p> <p>Nowadays, aCGH has become a very important tool, used in research of cancer or genomic disorders. Therefore, improving classification of aCGH data can have a great impact on many medical issues such as the process of diagnosis and finding disease-related genes. The performed experiment shows that the application of Jumping Emerging Patterns can be effective in the classification of high-dimensional data, including these from aCGH experiments.</p
Effect of Size and Heterogeneity of Samples on Biomarker Discovery: Synthetic and Real Data Assessment
MOTIVATION:
The identification of robust lists of molecular biomarkers related to a disease is a fundamental step for early diagnosis and treatment. However, methodologies for the discovery of biomarkers using microarray data often provide results with limited overlap. These differences are imputable to 1) dataset size (few subjects with respect to the number of features); 2) heterogeneity of the disease; 3) heterogeneity of experimental protocols and computational pipelines employed in the analysis. In this paper, we focus on the first two issues and assess, both on simulated (through an in silico regulation network model) and real clinical datasets, the consistency of candidate biomarkers provided by a number of different methods.
METHODS:
We extensively simulated the effect of heterogeneity characteristic of complex diseases on different sets of microarray data. Heterogeneity was reproduced by simulating both intrinsic variability of the population and the alteration of regulatory mechanisms. Population variability was simulated by modeling evolution of a pool of subjects; then, a subset of them underwent alterations in regulatory mechanisms so as to mimic the disease state.
RESULTS:
The simulated data allowed us to outline advantages and drawbacks of different methods across multiple studies and varying number of samples and to evaluate precision of feature selection on a benchmark with known biomarkers. Although comparable classification accuracy was reached by different methods, the use of external cross-validation loops is helpful in finding features with a higher degree of precision and stability. Application to real data confirmed these results
Performance of the ATLAS electromagnetic calorimeter end-cap module 0
The construction and beam test results of the ATLAS electromagnetic end-cap calorimeter pre-production module 0 are presented. The stochastic term of the energy resolution is between 10% GeV^1/2 and 12.5% GeV^1/2 over the full pseudorapidity range. Position and angular resolutions are found to be in agreement with simulation. A global constant term of 0.6% is obtained in the pseudorapidity range 2.5 eta 3.2 (inner wheel)
Systems biology of host-mycobiota interactions: dissecting Dectin-1 and Dectin-2 signalling in immune cells with DC-ATLAS
Modelling the networks sustaining the fruitful coexistence between fungi and their mammalian hosts is becoming increasingly important to control emerging fungal pathogens. The C-type lectins Dectin-1 and Dectin-2 are involved in host defense mechanisms against fungal infection driving inflammatory and adaptive immune responses and complement in containing fungal burdens. Recognizing carbohydrate structures in pathogens, their engagement induces maturation of dendritic cells (DCs) into potent immuno-stimulatory cells endowed with the capacity to efficiently prime T cells. Owing to these properties, Dectin-1 and Dectin-2 agonists are currently under investigation as promising adjuvants in vaccination procedures for the treatment of fungal infection. Thus, a detailed understanding of events' cascade specifically triggered in DCs upon engagement is of great interest in translational research. Here, we summarize the current knowledge on Dectin-1 and Dectin-2 signalling in DCs highlighting similarities and differences. Detailed maps are annotated, using the Biological Connection Markup Language (BCML) data model, and stored in DC-ATLAS, a versatile resource for the interpretation of high-throughput data generated perturbing the signalling network of DC
Electronic European Language Portfolio
This is a proposal of digital implementation of a European Language Portfolio (ELP), useful for learners of foreign languages.
The Council of Europe has recently stated that language teaching and learning are key elements towards European integration and mobility.
This firm belief is at the root of the Common European Framework of Reference for languages (2001). One important aspect underlined by the Framework is the need for the European citizen to have a personal document describing all his/her linguistic experiences and certifications. This document is called the European Language Portfolio - ELP.
At present many European Institutions have already accepted the suggestions given by the Framework and have created different paper versions of the Portfolio adapting the European ELP format to their specific needs.
However the paper editions present some limits that may be overcome by a digital version of the ELP. We aim at creating a digital ELP for university students which would bring many advantages in terms of ODL