Effects of Tropical Legume (\u3cem\u3eStylosanthes cv.\u3c/em\u3e Campo Grande) Silage and Dietary Protein Levels on Animal Performance of Finishing Beef Cattle

Recently, the interest in legume silage for livestock systems has increased in tropical countries (Heinritz et al., 2012) because of the high inclusion of the protein sources in the concentrate used in feedlot diets, like soybean meal. Stylosanthes cv. Campo Grande was developed in Brazil and this cultivar has a good adaptation to tropical conditions, as soils with low fertility and high acidity (Fernandes et al., 2005). Recently, this legume has been indicated for silage making because of its nutritional value and previous results with beef cattle have showed similar performance and digestibility in comparison with corn silage (Souza et al., 2014). The objective of this study was to evaluate the effects of Stylosanthes cv. Campo Grande silage (StS) and corn silage (CS) with two dietary protein levels

Fermentation Profile of Signal Grass Silages Intercropped With Calopo or Fertilized With Nitrogen and Treated With Microbial Inoculant

Tropical grasses have characteristics that allow the occurrence of undesirable fermentations resulting in low quality silage. Inoculants can improve silage quality. Fermentation profile and microbial population of signal grass silages under four management (M), with and without microbial inoculant, were evaluated. The experiment was carried out in a 4 × 2 factorial scheme, with silages of signal grass managed without N application (control, 0N), fertilized with 50 kg ha-1 of N (50N), fertilized with 100 kg ha-1 of N (100N), or signal grassintercropped with calopo (Calopogonium mucunoides D.) (LEG), with inoculant (I) andwithout inoculant (WI), in a randomized block design, with two replications of area and two replications of treatment per block. Analysis of pH, organic acids, ethanol, ammonia nitrogen, and microbial populations were performed in the silages. The use of inoculant reduced the acetic acid concentration in the 100N treatment silages (20.1 to 12.8 g kg-1 of DM). The lowest concentration of butyric acid (BA) (2.33 g kg-1 DM) was observed in signal grass silage fertilized with 100 kg of N, without inoculant. In inoculated silages, the treatments 50N and LEG provided lower concentrations of BA in relation to the control. Silage from the uninoculated 50N treatment had the lowest concentration of N-NH3 in relation to the other silages. The lactic acid bacteria (LAB) population was not affected by the factors studied. The inoculant provided a reduction in the population of enterobacteria. No mold and yeast populations were detected in the silages investigated. Signal grass silage fertilized with N or intercropped with calopo, with microbial inoculant, had better fermentative characteristics and could be an option for strategic management of pastures in tropical areas

Performance of Beef Cattle Fed Diets Containing \u3cem\u3eStylosanthes\u3c/em\u3e and Corn Silages

Reports of the use of tropical legumes in silage production are scarce as these legumes have high contents of crude protein, low water soluble carbohydrate and high buffering capacity, which inhibit the production of silages with good fermentation and nutritional characteristics. Recent research has, however, shown that it is possible to produce good quality silages using tropical legumes (Pereira et al. 2012). Souza et al. (2012) concluded that silage produced from Stylosanthes cv. Campo Grande (Stylosanthes capitata + Stylosanthes macrocephala) at 60% proportion of the dry matter of the diet can replace corn silage in diets for beef cattle in feedlots without altering their intake and productive performance. However, the ideal proportion of this silage in the diets for beef cattle is still unknown. Thus, the objective of this study was to evaluate the intake and performance of beef cattle fed diets with Stylosanthes and corn silages

Chemical Composition and Fermentation Profile of Perennial Peanut and Marandu Grass Mixed Silages

Perennial peanut has high quality, evidenced by the improvement of animal production in grazing, due to good contents of crude protein and digestibility, which makes it one of the best alternatives for low cost feeding (Paganella and Valls 2002). Grass ensilage associated with legumes is considered an alternative to meet the protein demand of cattle in the livestock. However, due to limited information on the techniques of grass ensilage with tropical legumes, this research aimed to evaluate the chemical composition and the fermentation profile of perennial peanut and Marandu mixed grass silages, treated or not with bacterial inoculant

\u3cem\u3eStylosanthes\u3c/em\u3e cv. Campo Grande in Diets of Beef Cattle: Intake and Digestibility of Nutrients and Ruminal Fermentation

Recent studies have shown that it is possible to obtain well-fermented silages from tropical legumes (Heinritz et al., 2012). Souza et al. (2014) concluded that Stylosanthes cv. Campo Grande silage (StS); (Stylosantes capitata + S. macrocephala), when included in the proportion of 50% dietary dry matter (DM), could replace corn silage in diets of feedlot beef cattle without altering intake and performance. However, there is a lack of information about increasing proportion of StS that should be included in diets for beef cattle. Thus, the objective of this study was to evaluate the intake and digestibility of nutrients, and ruminal fermentation of beef cattle fed diets with Stylosanthes and concentrate in different levels

Intake and Digestibility of Nutrients of Corn and \u3cem\u3eStylosanthes\u3c/em\u3e Silages in Diets for Sheep

The low concentration of water soluble carbohydrates, the high buffering capacity and the low autochthonous population of lactic acid bacteria are limiting factors to legume ensiling. However, recent research with tropical legumes has shown that the silages presented an adequate fermentation profile (Liu et al. 2012; Silva et al. 2012; Pereira et al. 2012). Nevertheless, there are few studies on the use of stylosanthes silages cv. Campo Grande (Stylosanthes capitata + Stylosantes macrocephala) for sheep feeding. The objective of this study was to evaluate the intake and digestibility of nutrients of stylosanthes and corn silages in sheep diets

Cellular prion protein interaction with vitronectin supports axonal growth and is compensated by integrins

The physiological functions of the cellular prion protein, PrPC, as a cell surface pleiotropic receptor are under debate. We report that PrPC interacts with vitronectin but not with fibronectin or collagen. the binding sites mediating this PrPC-vitronectin interaction were mapped to residues 105-119 of PrPC and the residues 307-320 of vitronectin. the two proteins were co-localized in embryonic dorsal root ganglia from wild-type mice. Vitronectin addition to cultured dorsal root ganglia induced axonal growth, which could be mimicked by vitronectin peptide 307-320 and abrogated by anti-PrPC antibodies. Full-length vitronectin, but not the vitronectin peptide 307-320, induced axonal growth of dorsal root neurons from two strains of PrPC-null mice. Functional assays demonstrated that relative to wild-type cells, PrPC-null dorsal root neurons were more responsive to the Arg-Gly-Asp peptide (an integrin-binding site), and exhibited greater alpha v beta 3 activity. Our findings indicate that PrPC plays an important role in axonal growth, and this function may be rescued in PrPC-knockout animals by integrin compensatory mechanisms.Hosp Alemao Oswaldo Cruz, Ludwig Inst Canc Res, São Paulo, BrazilUniv São Paulo, Inst Quim, Dept Bioquim, BR-05508 São Paulo, BrazilHosp Canc, Ctr Tratamento & Pesquisa, São Paulo, BrazilUniv Fed Parana, Dept Patol Basica, BR-80060000 Curitiba, Parana, BrazilUniv Fed Parana, Dept Biol Celular, BR-80060000 Curitiba, Parana, BrazilUniversidade Federal de São Paulo, INFAR, BR-04023062 São Paulo, BrazilUniversidade Federal de São Paulo, INFAR, BR-04023062 São Paulo, BrazilWeb of Scienc

Deciding case by case on family presence in the emergency care service

Objective: To understand how physicians and nurses experience and perceive the presence of families in the emergency care service. Methods: This was a qualitative study that used symbolic interactionism as a theoretical reference, and grounded theory as a methodological reference. Twenty professionals participated – equally representing physicians and nurses - working in two emergency rooms located in the south of Brazil. Data were collected between October of 2016 and February of 2017, by means of interviews. Results: The existence of a social culture of family exclusion was identifi ed, widely diffused and practiced by professionals. However, families sometimes remain with their loved ones in the emergency room, since professionals analyze and decide “case by case”, considering different aspects throughout the care process. Conclusion: Multiple aspects are related in determining family presence during emergency care for physicians and nurses. Thus, a single directive on the presence of the family is not prudent. In fact, it is suggested that each health unit develop its protocols, considering local particularities.Objetivo: Compreender como médicos e enfermeiros vivenciam e percebem a presença da família no serviço de atendimento emergencial. Métodos: Estudo qualitativo que utilizou o Interacionismo Simbólico como referencial teórico e a Teoria Fundamentada nos Dados como referencial metodológico. Participaram 20 profi ssionais – divididos equitativamente entre médicos e enfermeiros – que atuavam em duas Salas de Emergência localizadas no Sul do Brasil. Os dados foram coletados entre outubro de 2016 e fevereiro de 2017, por meio de entrevistas. Resultados: Identifi cou-se a existência de uma cultura social de exclusão familiar, amplamente difundida e praticada pelos profi ssionais. Contudo, às vezes, as famílias permanecem com seus entes queridos na Sala de Emergência, visto que os profi ssionais analisam e decidem “caso a caso”, considerando diferentes aspectos ao longo do processo assistencial. Conclusão: Para médicos e enfermeiros múltiplos aspectos estão relacionados na determinação da presença familiar durante o atendimento emergencial. Assim, não é aconselhável uma diretiva única para a presença da família. Em realidade, sugere-se que cada unidade de saúde elabore seus protocolos considerando as particularidades locais.Objetivo: Comprender cómo médicos y enfermeros experimentan y perciben la presencia familiar en el servicio de atención de urgencias. Métodos: Estudio cualitativo, aplicando el Interaccionismo Simbólico como referencial teórico, y la Teoría Fundamentada en los Datos como referencial metodológico. Participaron 20 profesionales –equitativamente divididos entre médicos y enfermeros– actuantes en dos Servicios de Urgencias del Sur de Brasil. Datos recolectados de octubre 2016 a febrero 2017 mediante entrevistas. Resultados: Se identifi có la existencia de una cultura social de exclusión familiar, ampliamente difundida y practicada por los profesionales. Igualmente, a veces, las familias permanecen con sus seres queridos en el Servicio de Urgencias, dado que los profesionales analizan y deciden “caso por caso”, considerando diferentes aspectos a lo largo del proceso de atención. Conclusión: Para médicos y enfermeros, múltiples aspectos se relacionan con la determinación de la presencia familiar durante la atención de urgencia. Por ello, no es aconsejable una directiva única sobre presencia familiar. En realidad, se sugiere que cada unidad de salud elabore sus protocolos considerando sus propias características

Microbial population, fermentative characteristics and dry matter recovery of mixed silage grass xaraés and stylo Campo Grande

The population of microorganisms, the fermentative characteristics and the recovery of dry matter of silage of xaraés grass (Brachiaria brizantha) with growing levels of Styansanthes capitata x S. macrocephala (0; 25; 50; 75) and 100%), with and without microbial inoculant. The 5 x 2 factorial scheme (five proportions of Campo Grande stylos with and without inoculant) was used in the completely randomized design, with three replications. There was no effect of the interaction of levels of stylos and microbial inoculant for the studied variables, however, it was observed effect of stylos levels on pH and ammoniacal nitrogen and inoculant on dry matter recovery. The mean population of lactic acid bacteria in the silages was 6.8 log cfu/g. The values of pH and ammoniacal nitrogen decreased linearly with the increase of the stylos levels in the silage. Mean dry matter recovery was 94%. It is concluded that the use of legume together with grass for silage production improves the fermentation characteristics and provides high recovery of dry matter, however, the inoculant evaluated does not alter the fermentative characteristics of the silages

Flexibility of C 4 decarboxylation and photosynthetic plasticity in sugarcane plants under shading

The flexibility between C4 photosynthetic sub-types NADP-malic enzyme (NADP-ME) and phosphoenolpyruvate carboxykinase (PEPCK), recently identified in some C4 species, confers high photosynthetic efficiency under varying light conditions. Theoretically, PEPCK decarboxylation uses less quanta per CO2 fixed than NADP-ME, suggesting an increase in PEPCK activity could be advantageous under shading, as CO2 leakiness increases under low light. Thus, we hypothesize that sugarcane plants have flexibility among the decarboxylation pathways, i.e., more than one decarboxylation route occurs independent of the environmental condition; furthermore, low light availability induces biochemical and anatomical adjustments resulting in increased PEPCK activity, which could contribute to maintaining or even increasing quantum efficiency of CO2 assimilation under limiting light. Two sugarcane varieties were evaluated and both presented activities of the three decarboxylases, either under full sunlight or shading. In vitro PEPCK activity increased in plants grown under low light, suggesting an upregulation of this decarboxylation pathway. Accordingly, changes in chloroplast arrangement of bundle sheath cells from centrifugal to evenly distributed were found. Our data suggest that such biochemical and anatomical adjustments found in sugarcane grown under shading were important to maintain the maximum quantum efficiency of CO2 assimilation. Finally, we propose a model highlighting the integration between the decarboxylation pathways under shading, considering carboxylation and decarboxylation pathways in sugarcane plants