Dynamic Fibrillar Assembly of αb-Crystallin Induced by Perturbation of the Conserved NT-IXI Motif Resolved by Cryo-Em

αB-crystallin is an archetypical member of the small heat-shock proteins (sHSPs) vital for cellular proteostasis and mitigating protein misfolding diseases. Gaining insights into the principles defining their molecular organization and chaperone function have been hindered by intrinsic dynamic properties and limited high-resolution structural analysis. To disentangle the mechanistic underpinnings of these dynamical properties, we mutated a conserved IXI-motif located within the N-terminal (NT) domain of human αB-crystallin. This resulted in a profound structural transformation, from highly polydispersed caged-like native assemblies into a comparatively well-ordered helical fibril state amenable to high-resolution cryo-EM analysis. The reversible nature of the induced fibrils facilitated interrogation of functional effects due to perturbation of the NT-IXI motif in both the native-like oligomer and fibril states. Together, our investigations unveiled several features thought to be key mechanistic attributes to sHSPs and point to a critical significance of the NT-IXI motif in αB-crystallin assembly, dynamics and chaperone activity

A descriptive study of International Special Olympic athletes\u27 vision care needs

Three hundred-seventy International Special Olympics participants and 115 staff members representing 39 countries participated . in a vision screening conducted by 32 members of the \u27 American Optometric Association\u27s Sports . Vision and/or Low Vision Sections during the 1991 International. Special Olympics, held in Minneapolis/St. Paul. The. program also included emergency eyecare services and educational· benefits relating to the role of vision in athletic and life performance. An age-matched population of normal nonathlete students were · compared on several measures of visual sensitivity and visual-motor performance. Results of the program substantiated the findings of the 1984-86 Oregon · State Special Olympics vision care program. An unmet need for comprehensive eyecare exists within the International Special Olympics population. Areas of greatest concern include the large number of competitors\u27 never having received vision care, nearly 113 of\u27 the athletes participate with uncompensated or. residual refractive error, and the increased risk of ocular trauma resulting from lack of · eye protection requirement

Comparative study of the blind spot locations between right and left eyes

A study of the blind spots of 42 college students revealed no clinically significant difference in their location when comparing the right eye to the left eye. The mean difference at the nerve head is zero, with an absolute standard deviation of the difference equal to 0.46. This finding demonstrates the high degree of symmetry in the location of the blind spots for individual subjects

A barreira costeira holocênica e suas relações com a morfodinâmica praial no Estado do Rio Grande do Sul, Brasil

A análise dos parâmetros morfométricos publicados buscou relacionar o comportamento morfodinâmico praial com o da barreira costeira holocênica no estado do Rio Grande do Sul (RS), para estabelecer de que maneira estes ambientes estão relacionados. Foram analisados os dados de 31 praias, de Torres ao Chuí, possibilitando uma revisão e reavaliação dos estágios morfodinâmicos, com a compartimentação destes locais em cinco grupos, baseados em sua morfodinâmica e mobilidade praial. O grupo 1 é composto pelas praias intermediárias de mobilidade moderada à alta, que estão em sua maioria localizadas no litoral médio do RS correspondente a barreira agradacional, com alto desenvolvimento de campos de dunas transgressivas e dunas frontais, e estão também associadas às praias com característica retrogradante da barreira, porção sul da barreira agradacional. O grupo 2 compõe as praias dissipativas, com estágio intermediário ocorrendo de maneira secundária, de moderada à alta mobilidade, associadas a barreira progradante do litoral norte. Este setor apesar de apresentar alto potencial de transporte sedimentar por ventos e por ondas não apresenta campos de dunas bem desenvolvidos, onde o aporte sedimentar favorece a progradação costeira. O grupo 3 é formado por praias intermediárias de baixa mobilidade, associadas à porção norte da barreira agradacional e às praias do litoral sul que representam a transição entre a barreira progradante e retrogradante. No litoral médio a associação da baixa mobilidade praial com a alta capacidade de transporte e disponibilidade sedimentar, resultante do transporte sedimentar pelo vento no sentido continental, permitiu o desenvolvimento de grandes campos de dunas transgressivos e de dunas frontais. O grupo 4 é constituído por praias dissipativas e secundariamente intermediárias, de baixa mobilidade, da barreira progradante do litoral sul, que não desenvolveu vastos campos de dunas, semelhante ao que ocorre na barreira progradante ao norte. O grupo 5 é formado por duas praias diferenciadas, onde o estágio intermediário predomina e o refletivo ocorre eventualmente no verão, e estão localizadas em barreiras retrogradantes. As praias dissipativas são responsáveis por remobilizar o maior volume sedimentar da antepraia ao ambiente praial, onde integra o processo de progradação costeira. A porção da barreira agradacional, responsável pelas maiores dunas frontais, apresenta a menor mobilidade, sendo que conforme sua mobilidade é aumentada em direção ao sul, os maiores campos de dunas transgressivas ocorrem. Os estágios intermediários possuem energia suficiente para transportar sedimentos da antepraia para a praia, enquanto que a formação ou não das dunas é influenciada pelo potencial de transporte do vento NE e pela orientação da linha de costa com relação a este vento. As barreiras retrogradantes estão associadas a estágios intermediários de mobilidade variável, sendo que os dois focos erosivos do estado estão associados à alta mobilidade, o qual deixa estes ambientes susceptíveis aos episódios de erosão durante a passagem de eventos de tempestades. Assim, o caráter erosivo da barreira pode estar associado tanto com a energia de ondas, quanto com uma antepraia irregular e um déficit sedimentar, que acarreta em variações na morfodinâmica praial.The analysis of the morphometric parameters of the published works about beach morphodynamics in the State of Rio Grande do Sul had the objective to relate the morphodynamic behavior of the beach with the holocene coastal barrier, in order to establish how these environments are related. The data of 31 beaches were analyzed, allowing a review and reevaluation of the morphodynamic stages, with the compartmentalization of these sites in five groups, based on their morphodynamics and beach mobility. Group 1 is composed of intermediate beaches with moderate to high mobility, which are located in the middle coast corresponding to the aggradational barrier, with high development of transgressive dunes and foredunes, and, associated too with the beaches with retrogradational characteristics of the barrier. Group 2 consists of dissipative beaches, with intermediate stage occurring in a secondary way, being these from moderate to high mobility, associated with the progradational barrier of the north coast. This sector, despite to present a high potential for sediment transport by winds and waves, does not have well development dunefields, where the sediment budget supports coastal progradation. Group 3 is formed by intermediate beaches of low mobility, associated with the northern portion of the aggradational barrier and the beaches of the south coast that represent the transition between the prograded and retrograded barrier. In the middle coast, the association of low beach mobility with high transport capacity and sedimentary availability, and the resulting of the sedimentary transport by wind onshore, allowed the development of large transgressive dunefields and foredunes. Group 4 consists of dissipative and secondarily intermediate beaches, with low mobility, of the progradational barrier of the south coast, which, similar to what occurs in the progradational barrier of the north, did not develop largest dune fields. Group 5 is formed by two beaches with peculiar characteristics, where the intermediate stage predominates and the reflective occurs eventually in the summer, associated with the retrograded barrier. The dissipative beaches are responsible for remobilizing highs sediments volumes of the shoreface and provide to the beach environment, where, in this case, it is converted into coastal progradation. The portion of the aggradational barrier responsible for the largest frontal dunes presents the smaller mobility, and as its mobility is increased towards the south, the largest transgressive dunefields in the state occurs. The intermediate stages have enough energy to carry sediments from the shoreface to the beach, while the formation of the dunes is influenced by the transport potential of the NE wind and by the orientation of the coastline in relation to this wind. Retrograded barriers are associated to intermediary stages of variable mobility, and the two erosive hotspots of the state are associated with high mobility, which turns these environments susceptible to erosion episodes during the passing of storm events. Thus, the erosive character of the barrier may be associated with both wave energy and an irregular shoreface and a negative sediment budget, which leads to variations in beach morphodynamics

Gegenwärtige Wanderungsbewegungen und Prognosen für Deutschland.

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Pragmatic Assessment in Autism Spectrum Disorders: A Comparison of a Standard Measure with Parent Report

The purpose of this study was to investigate the concurrent validity of subtests on the Comprehensive Assessment of Spoken Language (CASL) by comparing them with the assessment of communication and social skills on the Vineland Adaptive Behavior Scales (Vineland). The participants were 35 children and adolescents with higher functioning autism spectrum disorders (ASD) who had received both the CASL and the Vineland. Results of the study suggest that the Pragmatic Judgment and Inferences subtests of the CASL appeared to document the difficulties that individuals with ASD had in adaptive use of language for communication

The Structure and Function of Small Nucleolar Ribonucleoproteins

Eukaryotes and archaea use two sets of specialized ribonucleoproteins (RNPs) to carry out sequencespecific methylation and pseudouridylation of RNA, the two most abundant types of modifications of cellular RNAs. In eukaryotes, these protein–RNA complexes localize to the nucleolus and are called small nucleolar RNPs (snoRNPs), while in archaea they are known as small RNPs (sRNP). The C/D class of sno(s)RNPs carries out ribose- 20-O-methylation, while the H/ACA class is responsible for pseudouridylation of their RNA targets. Here, we review the recent advances in the structure, assembly and function of the conserved C/D and H/ACA sno(s)RNPs. Structures of each of the core archaeal sRNP proteins have been determined and their assembly pathways delineated. Furthermore, the recent structure of an H/ACA complex has revealed the organization of a complete sRNP. Combined with current biochemical data, these structures offer insight into the highly homologous eukaryotic snoRNPs

Espiritualidade cristã como resposta ao individualismo

The contemporary society has been characterized by individualism, the present trace that points to the centrality of the self and of the subjectivity. At this culture, ruled by narcissism, the relationships with the others are more and more self-referential, and it is the economical self, in its capacity to buy that has been determining who I am. It is believed that human longings are answered by particular choices to which the market offers countless consume options. In this way, consumerism grows promising to deliver prestige and to define identities, in an anxious and shredder dynamics. In this article, we investigate the Christian spirituality as a possibility of answering to the individualistic human of this consumerist society. The analysis is based on bibliographic research. The Christian spirituality puts these consumers in front of the reality of creation and answers to their most profound anxieties, teaching them about the God who is the centre, in the opposite way of individualism. In this perspective, they learn about the Creator God, that gives them the creature identity, fully and unique, that reveals itself in the necessity and the beauty of the relationship with the otherness.A sociedade contemporânea é caracterizada pelo individualismo, o traço da atualidade que aponta a centralidade do eu e da subjetividade. Em tal cultura, pautada pelo narcisismo, as relações com o outro são cada vez mais autorreferenciais, e é o eu econômico, na sua capacidade de comprar, que tem determinado quem eu sou. Acredita-se que os anseios humanos são respondidos pelas escolhas particulares, para as quais o mercado oferece inúmeras possibilidades de consumo. Assim, o consumismo cresce sob a promessa de entregar prestígio e definir identidades, em uma dinâmica ansiosa e fragmentadora. Neste artigo, investiga-se a espiritualidade cristã como uma possibilidade de resposta ao ser humano individualista desta sociedade consumista. A análise é baseada em pesquisa bibliográfica. A espiritualidade cristã coloca esse consumidor frente a relidade da criação e responde os seus anseios mais profundos, ensinando-lhe do Deus que é centro, diferente do que prega o individualismo. Nesta perspectiva, ele aprende do Deus Criador, que lhe entrega a identidade de criatura, plena e única, que se revela na necessidade e na beleza do relacionamento com a alteridade

King-Devick Test Score is Linked to Cerebral Vasoreactivity During Acute Recovery Phase Post-Concussion

The King-Devick (KD) test is a quick screening tool used to detect oculomotor and attention related brain function impairments following a concussion. Adequate cerebral blood flow (CBF) is an indicator of structural and functional integrity and impairments in CBF have been linked to cognitive deficits following concussion. Cerebral vasoreactivity (CVR), a key measure of hemodynamic reserve, is an important factor in CBFregulation. PURPOSE: To examine the association between CVR and oculomotor and attention function, using KD test scores, in collegiate athletes following a concussion. METHOD: Eighteen male and female athletes diagnosed with a sports-related concussion were enrolled in the prospective cohort study. Twenty-two non-injured athletes were enrolled as controls. Data was collected longitudinally on day-3, day-21, and day-90 following a concussion. Middle cerebral artery blood velocity (MCAV) was obtained using transcranial Doppler ultrasonography (TCD). CO2 was used as a stimulus to assess CVR. End-tidal CO2 (EtCO2) was monitored with an infrared CO2 analyzer attached to a nasal cannula. Continuous MCAV was obtained for 2-minutes each in response to three CO2 stimuli; normal room-air (normocapnia), inspiring 8% CO2 and 21% O2 gas mixture (hypercapnia), and during hyperventilation (hypocapnia). MCAV and EtCO2 data were plotted and CVR was estimated as the slope of the MCAV and EtCO2 relationship. For the KD test, subjects read aloud single-digit-numbers left to right from three test cards with progressive level of difficulty. Subjects were instructed to read as fast as possible without making errors. KD test score was determined as the sum of the total time required to complete the three test cards. Nonparametric Wilcoxon test was used to assess CVR and KD test scores between control and the concussed group at each of the three time points. Spearman rank order correlation was used to assess the association between CVR and KD scores at each time point. RESULTS: Compared to the control, CVR (1.33±0.31vs.1.14±0.43U; p=0.03) was blunted and KD score (44.4±7.7vs.48.1±6.9sec; p=0.04) was slower on day-3. CVR was comparable to the controls on days 21 and 90. However, a learning effect was observed in KD test score over time (42.1±8.9, p=0.02 [day-21]; 42.03±8.5, p=0.03 [day90]). In addition, a negative correlation between CVR and KD test was observed on day-3 (p= 0.04). CONCLUSION: The preliminary results indicate that deficits in oculomotor function and attention, as identified by KD test, may result from inadequate CBF regulation during the acute recovery phase post-concussion. Further studies should be done to evaluate the potential role of CVR in oculomotor function following concussion in collegiate athletes

Baroreflex Sensitivity is Impaired in Athletes Following a Sports-Related Concussion

Sport-related concussions are a major public health concern, with approximately 3.8 million incidences occurring annually in the United States alone. Autonomic nervous system (ANS) dysfunction is implicated in early and later stages of sports-related concussion recovery. Arterial baroreflex, a crucial mechanism by which the ANS controls short-term fluctuations in blood pressure, remains understudied in this population. PURPOSE: Examine baroreflex sensitivity (BRS) at rest during acute and sub-acute recovery phases following a sport-related concussion in collegiate athletes in comparison to non-injured athletes as controls. METHODS: Athletes (20±1 years) with sports-related concussions were tested on days 3 (N=13), 21 (N=13), and 90 (N=11) following the injury. Control athletes (N=12) were assessed at one time-point. Continuous arterial blood pressure (finger photoplethysmography) and R-R intervals (3-lead electrocardiogram) were obtained at rest for 6 minutes and while subjects were seated in an upright position. BRS was estimated with transfer function analysis to assess the fluctuations in systolic blood pressure and R-R intervals during the time period. Transfer gain in the low-frequency range (0.05– 0.15 Hz) quantified the magnitude of the relationship between changes in systolic blood pressure and R-R interval. Therefore, higher gain indicates higher BRS. A linear mixed model was used to examine symptoms and transfer function variables between the controls and the concussed athletes at the three time points. RESULTS: As anticipated, symptoms were worse on day-3 and resolved during the day-21 sub-acute phase. BRS was lower on day-3 (0.656±0.2U; p=0.003), day-21 (0.711±0.29U; p=0.013), and day-90 (0.77±0.27; p=0.04) following the injury compared to the controls (1.05±0.3U). CONCLUSION: The findings confirm impairments in baroreflex sensitivity during the acute and subacute recovery phases following a concussion despite symptom resolution. Blunted baroreflex sensitivity following injury may position athletes in a vulnerable situation while performing tasks that elicit sudden changes in blood pressure on and off the field