The Relationship between Endothelial Nitric Oxide Synthase Gene Polymorphism (G894T) and Isole Coronary Artery Ectasia

29th Turkish Cardiology Congress of the Turkish-Society-of-Cardiology (TSC) with International Participation -- OCT 26-29, 2013 -- Antalya, TURKEYWOS: 000329858400493…Turkish Soc Cardio

Clinical biochemistry laboratory rejection rates due to various types of preanalytical errors

Introduction: Preanalytical errors, along the process from the beginning of test requests to the admissions of the specimens to the laboratory, cause the rejection of samples. The aim of this study was to better explain the reasons of rejected samples, regarding to their rates in certain test groups in our laboratory. Materials and methods: This preliminary study was designed on the rejected samples in one-year period, based on the rates and types of inappropriateness. Test requests and blood samples of clinical chemistry, immunoassay, hematology, glycated hemoglobin, coagulation and erythrocyte sedimentation rate test units were evaluated. Types of inappropriateness were evaluated as follows: improperly labelled samples, hemolysed, clotted specimen, insufficient volume of specimen and total request errors. Results: A total of 5,183,582 test requests from 1,035,743 blood collection tubes were considered. The total rejection rate was 0.65 %. The rejection rate of coagulation group was significantly higher (2.28%) than the other test groups (P < 0.001) including insufficient volume of specimen error rate as 1.38%. Rejection rates of hemolysis, clotted specimen and insufficient volume of sample error were found to be 8%, 24% and 34%, respectively. Total request errors, particularly, for unintelligible requests were 32% of the total for inpatients. Conclusions: The errors were especially attributable to unintelligible requests of inappropriate test requests, improperly labelled samples for inpatients and blood drawing errors especially due to insufficient volume of specimens in a coagulation test group. Further studies should be performed after corrective and preventive actions to detect a possible decrease in rejecting sample

Relation of leptin, adiponectin and insulin resistance to bone mineral density in type 2 diabetic postmenopausal women

Wstęp: Celem badania było porównanie zależności między stężeniami leptyny i adiponektyny oraz insulinoopornością a gęstością mineralną kości (BMD) u kobiet po menopauzie chorujących na cukrzycę typu 2 i u osób z niechorującej na cukrzycę grupy kontrolnej. Materiał i metody: Do badania zrekrutowano łącznie 76 kobiet po menopauzie, które podzielono na grupy obejmujące kobiety chorujące na cukrzycę typu 2 (n = 19) i bez cukrzycy (n = 19), u których wartość T score dla BMD kręgosłupa i/lub bliższego odcinka kości udowej wynosiło mniej niż -2 oraz chore na cukrzycę typu 2 (n = 20) i niechorujące na cukrzycę (n = 18) z prawidłowymi wartościami BMD (T score > -1). Kryteria wykluczenia obejmowały stosowanie leków przeciwosteoporotycznych, wiek ponad 65 lat, choroby lub terapie mogące wpływać na metabolizm kostny. U uczestniczek badania wykonano badania biochemiczne, oznaczono stężenia leptyny, adiponektyny i insuliny oraz oszacowano stopień insulinooporności HOMA. Wyniki: Nie zaobserwowano korelacji między niskimi wartościami BMD a stężeniami leptyny i adiponektyny oraz insulinoopornością. Stwierdzono jedynie odwrotną zależność między stężeniem leptyny a BMD kości udowej w obrębie trójkąta Warda. Wnioski: Konieczne są dalsze badania obejmujące dużą grupę chorych, aby ustalić wpływ stężeń leptyny i adiponektyny oraz insulinooporności na metabolizm kostny u chorych na cukrzycę typu 2. (Endokrynol Pol 2011; 62 (5): 429&#8211;435)Background: Our aim is to identify the relation of leptin, adiponectin and insulin resistance to bone mineral density (BMD) in type 2 diabetic postmenopausal women and compare it with that experienced by nondiabetics. Material and methods: Seventy six postmenopausal female patients were included in the study. Postmenopausal type 2 diabetic (n = 19) and nondiabetic patients (n = 19) with spine and/or hip BMD T score lower than -2 were included in the study, and postmenopausal type 2 diabetic (n = 20) and nondiabetic women (n = 18) with normal BMD (T score > &#8211;1) were selected as control groups. Those receiving therapy for osteoporosis, over the age of 65, those who had a disease and were taking a medication that could affect bone metabolism were excluded. Biochemical tests, as well as leptin, adiponectin and insulin levels, were measured and insulin resistance was calculated using the HOMA test. Results: There was no correlation between low BMD and leptin, adiponectin and insulin resistance. There was only a negative correlation between leptin and femur Ward&#8217;s triangle BMD. Conclusion: Further large-scale studies must to be performed in order to analyse the effects of leptin, adiponectin and insulin resistance on bone metabolism in type 2 diabetic patients. (Pol J Endocrinol 2011; 62 (5): 429&#8211;435

Prevalence of Thrombophilic Mutations in Ischemic Stroke Patients in Isparta, Turkey

Objective: The present study aimed to investigate whether the frequency of factor V, methylenetetrahydrofolate reductase (MTHFR), prothrombin, beta-fibrinogen gene mutations, and human platelet alloantigens (HPA), plasminogen activator inhibitor 1 (PAI1), apolipoprotein E (APOE), and angiotensin converting enzyme (ACE) gene polymorphisms in stroke patients is higher than that in normal individuals

Effects of Subchronic Treatment with Ibuprofen and Nimesulide on Spatial Memory and NMDAR Subunits Expression in Aged Rats

Abstract Several studies point to an important function of cyclooxygenase (COX) and prostaglandin signaling in models of synaptic plasticity which is associated with N-methyl-D-aspartate receptors (NMDARs). Cyclooxygenase gene is suggested to be an immediate early gene that is tightly regulated in neurons by NMDA dependent synaptic activity. Nonsteroid Antiinflammatory Drugs (NSAIDs) exert their antiinflammatory effect by the inhibion of COX have controversial effects on learning and memory. We administered ibuprofen as a non-selective COX-2 inhibitor and nimesulide as a selective COX-2 inhibitor for 8 weeks for determining the cognitive impact of subchronic administration of NSAIDs to aged rats. Wistar albino rats (16 mo, n = 30) were separated into control (n = 10), ibuprofen (n = 10) and nimesulide (n = 10) treated groups. First we evaluated hippocampus-dependent spatial memory in the radial arm maze (RAM) and than we evaluated the expression of the NMDAR subunits, NR2A and NR2B by western blotting to see if their expressions are effected by subchronic administration with these drugs. Ibuprofen and nimesulide treated rats completed the task in a statistically significant shorter time when compared with control group (p &lt; 0.01), but there was no statistically significant difference between groups about choice accuracy data in RAM. Furthermore, no statistically significant difference was detected for the protein expressions of NR2A and NR2B of the subjects. Oral administration of ibuprofen and nimesulide for 8 weeks showed no impairment but partly improved spatial memory

Prothrombin Gene Mutation (G20210A) is Not Associated with Nonvalvular Atrial Fibrillation with Ischemic Stroke in Turkish Population

29th Turkish Cardiology Congress of the Turkish-Society-of-Cardiology (TSC) with International Participation -- OCT 26-29, 2013 -- Antalya, TURKEYWOS: 000329858400275…Turkish Soc Cardio

Endometrial flushing alpha(V)beta(3) integrin, glycodelin and PGF2 alpha levels for evaluating endometrial receptivity in women with polycystic ovary syndrome, myoma uteri and endometrioma

The aim of this cross-sectional study is to compare endometrial flushing fluid levels of alpha(V)beta(3) integrin, glycodelin and PGF2 alpha during the midluteal phase of the menstrual cycle of women with polycystic ovary syndrome (PCOS, n=20), myoma uteri (n=20) and endometrioma (n=19) with the healthy controls (n=20). After collecting samples at the midluteal phase of ovulatory volunteers and storing them at -80 degrees C, alpha(V)beta(3) integrin, glycodelin and PGF2 alpha levels were analyzed using ELISA. The mean ages of the groups were 28.90 +/- 5.45, 37.25 +/- 2.73, 32.84 +/- 6.62 and 32.15 +/- 5.18 in PCOS, myoma uteri, endometrioma and control groups, respectively. The alpha(V)beta(3) integrin level (ng/ml) was statistically significantly higher in endometrioma group (9.70 +/- 1.72, p<0.05) as compared to myoma uteri and control groups. Similarly, glycodelin level (ng/ml) was significantly higher in endometrioma group (341.04 +/- 93.32) than PCOS (p<0.01), myoma uteri (p<0.001) and healthy subjects (p<0.001). Moreover, PGF2 alpha level (350.04 +/- 464.50 ng/ml) was significantly higher in PCOS group relative to myoma uteri (p<0.001), endometrioma (p<0.05) and control (p<0.05) groups. In conclusion, alpha(V)beta(3) integrin level was significantly higher in endometrioma subjects than those with myoma uteri and control groups; glycodelin level was significantly higher in endometrioma group than other three groups, and lastly, PCOS patients had significantly higher PGF2 alpha levels than those patients with myoma uteri, endometrioma and controls

Evaluation of plasma oxidative status in patients with slow coronary flow

WOS: 000322658400008PubMed ID: 23797431Background: Slow coronary flow (SCF) is a coronary microvascular disorder characterised by delayed opacification of coronary vessels in a normal coronary angiogram. Coronary endothelial dysfunction plays an important pathogenetic role in patients with SCF. Oxidative stress is associated with cardiovascular diseases. Aim: To assess the total antioxidant capacity (TAC) and total oxidative status (TOS) in patients with SCF. Methods: The study included 36 patients with SCF. An age- and gender-matched control group was composed of 30 patients with normal coronary arteries and normal coronary flow. We measured plasma TAC and TOS levels and oxidative stress index (OSI) value in patients and control subjects. Linear regression analysis was performed to identify factors associated with the mean TIMI frame count (TFC). Results: Plasma TOS level and OSI value were significantly higher in the SCF group compared to the control group (p = 0.005 and p = 0.004, respectively). However, there was no significant difference in plasma TAC levels between the groups (p = 0.104). Factors associated with mean TFC were plasma TOS levels (beta = 0.425, p = 0.002) and fasting glucose levels (beta = 0.099, p = 0.01) in linear regression analysis. Conclusions: We found that plasma TOS and OSI were significantly higher in SCF compared to the control group and plasma TOS levels were independently associated with mean TFC

The Genetics Polymorphism of Beta-Fibrinogen 455 G/A in Atrial Fibrillation Patients with Ischemic Stroke

29th Turkish Cardiology Congress of the Turkish-Society-of-Cardiology (TSC) with International Participation -- OCT 26-29, 2013 -- Antalya, TURKEYWOS: 000329858400149…Turkish Soc Cardio

No Association between the Methyleletetrahydrofolate Reductase A1298C Variants and Atrial Fibrillation with Ischemic Stroke in Turkish Population

29th Turkish Cardiology Congress of the Turkish-Society-of-Cardiology (TSC) with International Participation -- OCT 26-29, 2013 -- Antalya, TURKEYWOS: 000329858400321…Turkish Soc Cardio