The Third Way for the Third Sector: Using Design to Transfer Knowledge and Improve Service in a Voluntary Community Sector Organisation

This paper describes a two-year Knowledge Transfer Partnership that concluded in September 2011. Knowledge Transfer Partnerships (KTP) is a UK-wide activity that helps organisations to improve their competitiveness and productivity by making better use of knowledge, technology and skills within universities, colleges and research organisations. This paper details the outcome of a KTP between Age UK Newcastle and Northumbria University’s School of Design that aimed to use Design approaches to improve the charity’s services. This paper will describe the recent context for organisations operating in the Voluntary Community Sector and discuss the relevance of a Design approach to both the improvement of customer services in this circumstance, as well as the transfer of knowledge to a capacity-starved organisation. It will also document how Design was used to achieve both of these aims, and the resulting impact of this engagement on the organisation and stakeholders

Comments on the optical lineshape function: Application to transient hole-burned spectra of bacterial reaction centers

Citation: Reppert, M., Kell, A., Pruitt, T., & Jankowiak, R. (2015). Comments on the optical lineshape function: Application to transient hole-burned spectra of bacterial reaction centers. Journal of Chemical Physics, 142(9), 7. doi:10.1063/1.4913685The vibrational spectral density is an important physical parameter needed to describe both linear and non-linear spectra of multi-chromophore systems such as photosynthetic complexes. Low-temperature techniques such as hole burning (HB) and fluorescence line narrowing are commonly used to extract the spectral density for a given electronic transition from experimental data. We report here that the lineshape function formula reported by Hayes et al. [J. Phys. Chem. 98, 7337 (1994)] in the mean-phonon approximation and frequently applied to analyzing HB data contains inconsistencies in notation, leading to essentially incorrect expressions in cases of moderate and strong electron-phonon (el-ph) coupling strengths. A corrected lineshape function L(omega) is given that retains the computational and intuitive advantages of the expression of Hayes et al. [J. Phys. Chem. 98, 7337 (1994)]. Although the corrected lineshape function could be used in modeling studies of various optical spectra, we suggest that it is better to calculate the lineshape function numerically, without introducing the mean-phonon approximation. New theoretical fits of the P870 and P960 absorption bands and frequency-dependent resonant HB spectra of Rb. sphaeroides and Rps. viridis reaction centers are provided as examples to demonstrate the importance of correct lineshape expressions. Comparison with the previously determined el-ph coupling parameters [Johnson et al., J. Phys. Chem. 94, 5849 (1990); Lyle et al., ibid. 97, 6924 (1993); Reddy et al., ibid. 97, 6934 (1993)] is also provided. The new fits lead to modified el-ph coupling strengths and different frequencies of the special pair marker mode, omega(sp), for Rb. sphaeroides that could be used in the future for more advanced calculations of absorption and HB spectra obtained for various bacterial reaction centers. (c) 2015 AIP Publishing LLC

Fluctuation limits of strongly degenerate branching systems

Functional limit theorems for scaled fluctuations of occupation time processes of a sequence of critical branching particle systems in $\R^d$ with anisotropic space motions and strongly degenerated splitting abilities are proved in the cases of critical and intermediate dimensions. The results show that the limit processes are constant measure-valued Wienner processes with degenerated temporal and simple spatial structures.Comment: 15 page

Development of Sstreamflow Projections Under Changing Climate Conditions Over Colorado River Basin Headwaters

The current drought over the Colorado River Basin has raised concerns that the US Department of the Interior, Bureau of Reclamation (Reclamation) may impose water shortages over the lower portion of the basin for the first time in history. The guidelines that determine levels of shortage are affected by relatively short-term (3 to 7 month) forecasts determined by the Colorado Basin River Forecast Center (CBRFC) using the National Weather Service (NWS) River Forecasting System (RFS) hydrologic model. While these forecasts by the CBRFC are useful, water managers within the basin are interested in long-term projections of streamflow, particularly under changing climate conditions. In this study, a bias-corrected, statistically downscaled dataset of projected climate is used to force the NWS RFS utilized by the CBRFC to derive projections of streamflow over the Green, Gunnison, and San Juan River headwater basins located within the Colorado River Basin. This study evaluates the impact of changing climate to evapotranspiration rates and contributes to a better understanding of how hydrologic processes change under varying climate conditions. The impact to evapotranspiration rates is taken into consideration and incorporated into the development of streamflow projections over Colorado River headwater basins in this study. Additionally, the NWS RFS is modified to account for impacts to evapotranspiration due to changing temperature over the basin. Adjusting evapotranspiration demands resulted in a 6% to 13% average decrease in runoff over the Gunnison River Basin when compared to static evapotranspiration rates. Streamflow projections derived using projections of future climate and the NWS RFS provided by the CBRFC resulted in decreased runoff in 2 of the 3 basins considered. Over the Gunnison and San Juan River basins, a 10% to 15% average decrease in basin runoff is projected through the year 2099. However, over the Green River basin, a 5% to 8% increase in basin runoff is projected through 2099. Evidence of nonstationary behavior is apparent over the Gunnison and San Juan River basins

Development of Streamflow Projections Under Changing Climate Conditions Over Colorado River Basin Headwaters

The current drought over the Colorado River Basin has raised concerns that the US Department of the Interior, Bureau of Reclamation (Reclamation) may impose water shortages over the lower portion of the basin for the first time in history. The guidelines that determine levels of shortage are affected by forecasts determined by the Colorado Basin River Forecast Center (CBRFC). While these forecasts by the CBRFC are useful, water managers within the basin are interested in long-term projections of streamflow, particularly under changing climate conditions. In this study, a bias-corrected, statistically downscaled dataset of projected climate is used to force a hydrologic model utilized by the CBRFC to derive projections of streamflow over the Green, Gunnison, and San Juan River headwater basins located within the Colorado River Basin. This study evaluates the impact of changing climate to evapotranspiration rates. The impact to evapotranspiration rates is taken into consideration and incorporated into the development of streamflow projections over Colorado River headwater basins in this study. Additionally, the CBRFC hydrologic model is modified to account for impacts to evapotranspiration due to changing temperature over the basin. Adjusting evapotranspiration demands over the Gunnison resulted in a 6% to 13% average decrease in runoff over the Gunnison River Basin when compared to static evapotranspiration rates. Streamflow projections derived using projections of future climate and the CBRFC’s hydrologic model resulted in decreased runoff in 2 of the 3 basins considered. Over the Gunnison and San Juan River basins, a 10% to 15% average decrease in basin runoff is projected through the year 2099. However, over the Green River basin, a 5% to 8% increase in basin runoff is projected through 2099. Evidence of nonstationary behavior is apparent over the Gunnison and San Juan River basins

NCBI Reference Sequences (RefSeq): current status, new features and genome annotation policy

The National Center for Biotechnology Information (NCBI) Reference Sequence (RefSeq) database is a collection of genomic, transcript and protein sequence records. These records are selected and curated from public sequence archives and represent a significant reduction in redundancy compared to the volume of data archived by the International Nucleotide Sequence Database Collaboration. The database includes over 16 000 organisms, 2.4 × 106 genomic records, 13 × 106 proteins and 2 × 106 RNA records spanning prokaryotes, eukaryotes and viruses (RefSeq release 49, September 2011). The RefSeq database is maintained by a combined approach of automated analyses, collaboration and manual curation to generate an up-to-date representation of the sequence, its features, names and cross-links to related sources of information. We report here on recent growth, the status of curating the human RefSeq data set, more extensive feature annotation and current policy for eukaryotic genome annotation via the NCBI annotation pipeline. More information about the resource is available online (see http://www.ncbi.nlm.nih.gov/RefSeq/)

THE COMPLEXITIES OF CHILDHOOD OBESITY: A QUALITATIVE STUDY AMONG MEXICAN AMERICAN MOTHERS

Introduction: Mothers play an important role in preventing childhood obesity; however, might differ in how they define healthy weight and the associated consequences. Due to the lack of research investigating how Mexican American mothers perceive childhood obesity, the purpose of this paper is to qualitatively assess Mexican-American mothers’ perceptions regarding childhood obesity, its causes and consequences. Methods: Three focus groups, two in Spanish and one in English, were conducted with 23 mothers in Bryan, TX. Thematic analysis was performed by three researchers independently using sentences as the unit of meaning. Findings: Mexican American mothers believed that a child needs to be a healthy weight to be healthy; however, the mothers varied in their methods for determining if their child was at a healthy weight. Mothers identified several underlying causes of childhood obesity including genetics, parenting, household characteristics and culture. Conclusion: Understanding the views of mothers of at-risk of rearing overweight children are important in elucidating effective strategies for preventing childhood obesity. Communication messages for this group might be more effective if they are contextualized within the culture, household and family

DNA replication stress restricts ribosomal DNA copy number

Ribosomal RNAs (rRNAs) in budding yeast are encoded by ~100–200 repeats of a 9.1kb sequence arranged in tandem on chromosome XII, the ribosomal DNA (rDNA) locus. Copy number of rDNA repeat units in eukaryotic cells is maintained far in excess of the requirement for ribosome biogenesis. Despite the importance of the repeats for both ribosomal and non-ribosomal functions, it is currently not known how “normal” copy number is determined or maintained. To identify essential genes involved in the maintenance of rDNA copy number, we developed a droplet digital PCR based assay to measure rDNA copy number in yeast and used it to screen a yeast conditional temperature-sensitive mutant collection of essential genes. Our screen revealed that low rDNA copy number is associated with compromised DNA replication. Further, subculturing yeast under two separate conditions of DNA replication stress selected for a contraction of the rDNA array independent of the replication fork blocking protein, Fob1. Interestingly, cells with a contracted array grew better than their counterparts with normal copy number under conditions of DNA replication stress. Our data indicate that DNA replication stresses select for a smaller rDNA array. We speculate that this liberates scarce replication factors for use by the rest of the genome, which in turn helps cells complete DNA replication and continue to propagate. Interestingly, tumors from mini chromosome maintenance 2 (MCM2)-deficient mice also show a loss of rDNA repeats. Our data suggest that a reduction in rDNA copy number may indicate a history of DNA replication stress, and that rDNA array size could serve as a diagnostic marker for replication stress. Taken together, these data begin to suggest the selective pressures that combine to yield a “normal” rDNA copy number

Towards reusable personas for everyday design

Personas are artificial character based representations of user goals, attitudes, motivations and abilities which enable designers to focus their design efforts on key, targeted users. The success of personas in design is due to their capacity to enable designers to empathize with users and understand user goals. Persona development is rooted in the rigorous collection and analysis of data specifically related to the design project being undertaken. New design projects thus require the development of new personas. Since redevelopment is not always achievable attention has turned towards reuse of personas and the underlying data. This paper reports on ongoing research into the development of reusable personas for use by non-expert, everyday designers. Such designers are regularly faced with small scale but diverse design challenges for which they cannot carry out user research and modelling. They can, however, make use of general, reusable personas developed independently of their current design project