Implementing the institutional data repository IST DataRep

In this report the implementation of the institutional data repository IST DataRep at IST Austria will be covered: Starting with the research phase when requirements for a repository were established, the procedure of choosing a repository-software and its customization based on the results of user-testings will be discussed. Followed by reflections on the marketing strategies in regard of impact, and at the end sharing some experiences of one year operating IST DataRep

Open Access at IST Austria 2009-2017

Poster Beitrag der Open-Access-Tage 2018 in Graz</p

Metadata for Research Data in Practice

Welche Daten über Daten brauchen wir? Die Beschreibung des Prozesses diese Frage für das institutionelle Datenrepositourium IST DataRep zu beantworten.What data is needed about data? Describing the process to answer this question for the institutional data repository IST DataRep

The influence of formal education, years of service and team meetings on the quality of interaction in Austrian centre-based settings for children under 3 years

Previous studies have reviewed positive correlations between the formal education levels of educators in Early Childhood Education and Care (ECEC) centres and the quality of interactions, but the findings have not been consistent (Early et al., 2007; Manning et al., 2017). Moreover, informal learning processes seem to be important too (Pianta et al., 2016). The present paper addresses this and explores how education levels of ECEC staff, years of service, and the frequency of team meetings relate to the quality of interactions in Austrian centre-based settings for children under 3 years. The interaction quality was measured among early childhood educators and assistants (N = 116) using the Graz Scale of Interaction Quality for Children between 0 and 3 years (GrazIAS 0-3) (Walter-Laager, Flöter et al., 2019). The results of multiple regression models indicate that the frequency of team meetings strongly positively correlates with both the subscales of interaction quality, 'ensure relationships and wellbeing' and 'support learning'. Further, the level of education of the ECEC staff and their years of service positively correlate with the subscale 'support learning' with low-to-medium effect sizes. The findings also suggest that team meetings might be important for increasing the quality of interactions at ECEC centres.Previous studies have reviewed positive correlations between the formal education levels of educators in Early Childhood Education and Care (ECEC) centres and the quality of interactions, but the findings have not been consistent (Early et al., 2007; Manning et al., 2017). Moreover, informal learning processes seem to be important too (Pianta et al., 2016). The present paper addresses this and explores how education levels of ECEC staff, years of service, and the frequency of team meetings relate to the quality of interactions in Austrian centre-based settings for children under 3 years. The interaction quality was measured among early childhood educators and assistants (N = 116) using the Graz Scale of Interaction Quality for Children between 0 and 3 years (GrazIAS 0-3) (Walter-Laager, Flöter et al., 2019). The results of multiple regression models indicate that the frequency of team meetings strongly positively correlates with both the subscales of interaction quality, 'ensure relationships and wellbeing' and 'support learning'. Further, the level of education of the ECEC staff and their years of service positively correlate with the subscale 'support learning' with low-to-medium effect sizes. The findings also suggest that team meetings might be important for increasing the quality of interactions at ECEC centres

IST PubRep und IST DataRep: die institutionellen Repositorien am IST Austria

Am IST Austria wurden 2013 ein Publikationsrepositorium und 2015, nach einer sorgfältigen Vorbereitungsphase, ein Datenrepositorium in Betrieb genommen – beide auf der Open-Source-Software EPrints basierend. Im ersten Teil unseres Erfahrungsberichts werden wir aus gegebenem Anlass unsere Erfahrungen mit Open-Source-Software allgemein und EPrints im Besonderen, bezüglich technischer Aspekte als auch im Hinblick ihrer Besonderheit – der User Communities, rekapitulieren. Der zweite Teil ist als Plädoyer für die Einbeziehung von EndbenutzerInnen in die Implementierungs-, Anpassungs- und Evaluierungsprozesse konzipiert

IST PubRep und IST DataRep: die institutionellen Repositorien am IST Austria

In 2013, a publication repository was implemented at IST Austria and 2015 after a thorough preparation phase a data repository was implemented – both based on the Open Source Software EPrints. In this text, designed as field report, we will reflect on our experiences with Open Source Software in general and specifically with EPrints regarding technical aspects but also regarding their characteristics of the user community. The second part is a pleading for including the end users in the process of implementation, adaption and evaluation

IST PubRep und IST DataRep: die institutionellen Repositorien am IST Austria

In 2013, a publication repository was implemented at IST Austria and 2015 after a thorough preparation phase a data repository was implemented – both based on the Open Source Software EPrints. In this text, designed as field report, we will reflect on our experiences with Open Source Software in general and specifically with EPrints regarding technical aspects but also regarding their characteristics of the user community. The second part is a pleading for including the end users in the process of implementation, adaption and evaluation

Concurrent MEK targeted therapy prevents MAPK pathway reactivation during BRAFV600E targeted inhibition in a novel syngeneic murine glioma model.

Inhibitors of BRAFV600E kinase are currently under investigations in preclinical and clinical studies involving BRAFV600E glioma. Studies demonstrated clinical response to such individualized therapy in the majority of patients whereas in some patients tumors continue to grow despite treatment. To study resistance mechanisms, which include feedback activation of mitogen-activated protein kinase (MAPK) signaling in melanoma, we developed a luciferase-modified cell line (2341luc) from a BrafV600E mutant and Cdkn2a- deficient murine high-grade glioma, and analyzed its molecular responses to BRAFV600E- and MAPK kinase (MEK)-targeted inhibition. Immunocompetent, syngeneic FVB/N mice with intracranial grafts of 2341luc were tested for effects of BRAFV600E and MEK inhibitor treatments, with bioluminescence imaging up to 14-days after start of treatment and survival analysis as primary indicators of inhibitor activity. Intracranial injected tumor cells consistently generated high-grade glioma-like tumors in syngeneic mice. Intraperitoneal daily delivery of BRAFV600E inhibitor dabrafenib only transiently suppressed MAPK signaling, and rather increased Akt signaling and failed to extend survival for mice with intracranial 2341luc tumor. MEK inhibitor trametinib delivered by oral gavage daily suppressed MAPK pathway more effectively and had a more durable anti-growth effect than dabrafenib as well as a significant survival benefit. Compared with either agent alone, combined BRAFV600E and MEK inhibitor treatment was more effective in reducing tumor growth and extending animal subject survival, as corresponding to sustained MAPK pathway inhibition. Results derived from the 2341luc engraftment model application have clinical implications for the management of BRAFV600E glioma

HIF1α Induces the Recruitment of Bone Marrow-Derived Vascular Modulatory Cells to Regulate Tumor Angiogenesis and Invasion

SummaryDevelopment of hypoxic regions is an indicator of poor prognosis in many tumors. Here, we demonstrate that HIF1α, the direct effector of hypoxia, partly through increases in SDF1α, induces recruitment of bone marrow-derived CD45+ myeloid cells containing Tie2+, VEGFR1+, CD11b+, and F4/80+ subpopulations, as well as endothelial and pericyte progenitor cells to promote neovascularization in glioblastoma. MMP-9 activity of bone marrow-derived CD45+ cells is essential and sufficient to initiate angiogenesis by increasing VEGF bioavailability. In the absence of HIF1α, SDF1α levels decrease, and fewer BM-derived cells are recruited to the tumors, decreasing MMP-9 and mobilization of VEGF. VEGF also directly regulates tumor cell invasiveness. When VEGF activity is impaired, tumor cells invade deep into the brain in the perivascular compartment

Pan-cancer analysis of the extent and consequences of intratumor heterogeneity

Intratumor heterogeneity (ITH) drives neoplastic progression and therapeutic resistance. We used the bioinformatics tools ‘expanding ploidy and allele frequency on nested subpopulations’ (EXPANDS) and PyClone to detect clones that are present at a >= 10% frequency in 1,165 exome sequences from tumors in The Cancer Genome Atlas. 86% of tumors across 12 cancer types had at least two clones. ITH in the morphology of nuclei was associated with genetic ITH (Spearman’s correlation coefficient, rho = 0.24-0.41; P 2 clones coexisted in the same tumor sample (HR = 1.49, 95% CI: 1.20-1.87). In two independent data sets, copy-number alterations affecting either 75% of a tumor’s genome predicted reduced risk (HR = 0.15, 95% CI: 0.08-0.29). Mortality risk also declined when > 4 clones coexisted in the sample, suggesting a trade-off between the costs and benefits of genomic instability. ITH and genomic instability thus have the potential to be useful measures that can universally be applied to all cancers