Science communication in bioengineering and biotechnology: Active and collaborative learning project

In a society increasingly dependent on science and technology, the need to equip our students with the most varied digital and communication skills is crucial. Active and collaborative learning among peers is essential for the acquisition of transversal skills. Communication is one of the main tools that the Engineer uses to reach his target audience. Science Communication in Bioengineering and Biotechnology (CCBioTec) is a project on Innovation and Development of Teaching and Learning supported by Center IDEA-UMinho, a structure that emerges to promote and value Innovation and Development of Teaching and Learning at the University of Minho. CCBioTec is transversal to a set of Learning Units (LU) under the responsibility of the Department of Biological Engineering (DEB), including one LU of each year of the Integrated Masters in Biological Engineering and in Biomedical Engineering. The main goals of CCBioTEc are: to foster the awareness of the DEB educational community on the importance of science communication, as well as to develop science communication skills, through the production of short videos (pitches) displaying the explanation, in a simple and dynamic way, of complex concepts of Bioengineering and Biotechnology related with the curricula of each LU. CCBioTec started in the second semester of 2020/21, and it will go on in the 1st semester of 2021/22. The project was designed to be implemented according to the following steps: 1 - Technical and pedagogical training of teachers; 2 - Technical training of students involved in the project - Week CCBioTEC-2021; 3 - Development of materials for Science Communication in Bioengineering and Biotechnology; 4 - CCBioTec-2021 competition. In CCBioTec, teachers presenting himself as a mediator/facilitator of learning, boosting students development of transversal skills, collaborative work, decision making and the expression of ideas, together with the acquisition of knowledge foreseen in the curricular contents of the LU.(undefined)info:eu-repo/semantics/publishedVersio

Microbial acclimation to concentrated human urine in Bio-electrochemical system

The aim of this study is to promote the gradual acclimation of bioelectroactive microorganisms in BES to concentrated human urine, and to assess different anode potentials and carbon materials in Microbial Electrolysis Cells (MEC). Human urine is highly concentrated in nutrients, representing more than 80% of the total N load and around 45% of the total P load in municipal wastewater. Separation of urine from other wastewater streams is an interesting option to keep these valuable nutrients concentrated, in order to develop a suitable nutrient recovery concept. This work is integrated in the Value from Urine (VFU) concept, where phosphate is recovered from source segregated human urine through struvite precipitation and ammonia is recovered in a Bio-electrochemical System (BES). Enrichment of an anaerobic sludge community in urine-degrading-electroactive microorganisms was promoted in an Microbial Fuel Cell (MFC) operated with increasing concentrations of real human urine (after phosphorous removal, as struvite). This acclimated electroactive biofilm was used to inoculate the anode of MECs, aiming at H2 and ammonia production in the cathode compartment. Different carbon modified anodes and defined anode potentials were assessed in terms of performance and microbial diversity of the developed electroactive biofilms

Bioelectrochemically-assisted recovery of valuable resources from urine

Book of Abstracts of CEB Annual Meeting 2017[Excerpt] Source separated urine is highly concentrated in nutrients and biodegradable compounds. This work explores the potential of combining nutrient recovery from urine with simultaneous energy production in bioelectrochemical systems (BES), under the FP7 project "ValueFromUrine". Non-spontaneous phosphorus (P) recovery by struvite precipitation was analysed by adding three different magnesium (Mg) sources (magnesium chloride (MgCl2), magnesium hydroxide (Mg(OH)2) and magnesium oxide (MgO)). A statistical design of experiments was used to evaluate the effect of Mg:P molar ratio (1:1, 1.5:1 and 2:1) combined with stirring speed (30, 45 and 60 rpm) for each Mg source tested. MgO at 2:1 molar ratio and a stirring speed of 30 rpm allowed to achieve the highest P recovery efficiency (99 %) with struvite crystals size of 50 to 100 μm [1]. Urine obtained after P recovery, showed high concentration of biodegradable compounds being subsequently fed as substrate in a microbial fuel cell (MFC). Microbial acclimation to urine was performed in a MFC resulting in an anaerobic community successfully enriched in “urine-degrading” electroactive microorganisms. When compared to the control assay operated without preliminary microbial enrichment (81±9 mA m-2), the acclimation method achieved significantly higher current density (455 mA m-2) (p<0.05). Tissierella and Paenibacillus were the dominant genus identified in the adapted microbial community. Tissierella can convert creatinine to acetate, whereas bacterial species belonging to the Paenibacillus genus are known to function as exoelectrogens. Corynebacterium that comprise urea-hydrolysing bacteria was also detected in the developed biofilms. [...]info:eu-repo/semantics/publishedVersio

An innovative bioelectrochemical system for the recovery of phosphorus, ammonia and electricity from urine

Ammonium and phosphate fertilizers are needed in agriculture to ensure a sufficient food production. The recovery of valuable nutrients (ammonium and phosphate) from waste(water) streams will help to overcome future shortages and reduce the need for phosphorous ore imports and energy intensive ammonia production. One person produces on average 1.5 L of urine per day, which contains about 9.1 g N /L and 1 g P /L. Urine contributes about 80% of the N load and 50% of the P load in conventional domestic wastewaters. These high nutrient concentrations in urine make it possible to develop more effective and energy efficient recovery technologies. In the ValueFromUrine project the phosphorus recovery will be performed by struvite precipitation from hydrolyzed urine and the resulting effluent will be used for ammonium recovery and simultaneously electricity generation in Bioelectrochemical systems. Bioelectrochemical systems (ie Microbial Fuel Cells) are engineered systems in which bacteria catalyze the oxidation of organic substrates and transfer electrons to anode and at the cathode oxygen is reduced. The aim of our project is to develop, demonstrate and evaluate an effective energy-efficient system for the recovery of nutrients from urine. Our treatment system will be able to recover >95% of the phosphorous (as struvite) and nitrogen (as struvite and ammonia / ammonium sulphate) while producing energy. These products can substitute salts used by the chemical industry, the artificial fertilizer industry and the agricultural sector which are currently obtained in a non-renewable and unsustainable wa

Effect of different carbon materials on the performance of microbial electrolysis cells (MECs) operated on urine and their microbial composition

ISMET 6 - General Meeting of the International Society for Microbial Electrochemistry and TechnologyUrine is rich in nitrogen and phosphorous and can considerably reduce domestic wastewater treatment requirements if collected separately. Source separated urine has been shown to be suitable for energy production and nutrients recovery in bioelectrochemical systems. However, there are still several challenges to overcome mainly related to organics conversion into electrical energy. In this study, anode performance of three microbial electrolysis cells (MECs) fed with urine using different carbon anodes, Keynol (phenolic-based), C-Tex (cellulose-based) and PAN (polyacrylonitrilebased) was compared. Two strategies were used to supply energy to the MECs; cell potential control (1' 1 assay) and anode potential control (2"d assay). In both assays, the C-Tex MEC outperformed MECs using Keynol and PAN. The C-Tex MEC with anode potential control at -0.300 V generated the highest current density of 904 mA m·2 , which was almost 3-fold higher than the MEC with Keynol, and 8-fold higher than the MEC with PAN. Analysis of anodes textural, chemical and electrochemical characteristics suggest that the higher external surface area of C-Tex enabled the higher current density generation compared to Keynol and PAN. The microbial composition on each anode and its correlation with the generated current was also investigated. No significant differences were observed in microbial diversity of the biofilm present in the studied anodes. Nonetheless, C-Tex had higher dominance of bacteria belonging to Luctobucillu/es and Enterobucteriules suggesting its relation with higher current generation.info:eu-repo/semantics/publishedVersio

Influence of carbon anode properties on performance and microbiome of Microbial Electrolysis Cells operated on urine

"Available online 15 February 2018"Anode performance of Microbial Electrolysis Cells (MECs) fed with urine using different anodes, Keynol (phenolic-based), C-Tex (cellulose-based) and PAN (polyacrylonitrile-based) was compared under cell potential control (1st assay) and anode potential control (2nd assay). In both assays, C-Tex MEC outperformed MECs using Keynol and PAN. C-Tex MEC under anode potential control (0.300V vs. Ag/AgCl) generated the highest current density (904mAm2), which was almost 3-fold higher than the Keynol MEC and 8-fold higher than the PAN MEC. Analysis of anodes textural, chemical and electrochemical characteristics suggest that the higher external surface area of C-Tex enabled higher current density generation compared to Keynol and PAN. Anodes properties did not influence significantly the microbial diversity of the developed biofilm. Nonetheless, C-Tex had higher relative abundance of bacteria belonging to Lactobacillales and Enterobacteriales suggesting its correlation with the higher current generation.This study was supported by the European Union's Seventh Programme for research, technological development and demonstration [Grant number 308535] and by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UID/BIO/04469/2013 unit and COMPETE 2020 [POCI-01- 0145-FEDER-006684], of Project RECI/BBB-EBI/0179/2012 [FCOMP01-0124-FEDER-027462], POCI-01-0145-FEDER-007679 [UID/CTM/ 50011/2013], and by BioTecNorte operation [NORTE-01-0145- FEDER-000004] funded by the European Regional Development Fund under the scope of Norte2020 - Programa Operacional Regional do Norte. This work is a result of project “AIProcMat@N2020 - Advanced Industrial Processes and Materials for a Sustainable Northern Region of Portugal 2020”, with the reference NORTE-01-0145-FEDER-000006, supported by Norte Portugal Regional Operational Programme (NORTE 2020), under the Portugal 2020 Partnership Agreement, through the European Regional Development Fund (ERDF) and of Project POCI-01-0145- FEDER-006984 e Associate Laboratory LSRE-LCM funded by ERDF through COMPETE2020 - Programa Operacional Competitividade e Internacionalizaçao (POCI) e and by national funds through FCT. The authors also would like to acknowledge the support of Wetsus, European Centre of Excellence for Sustainable Water Technology.info:eu-repo/semantics/publishedVersio

Electron scattering processes in Ho5(SixGe1−x)4 compounds: Electrical resistivity studies

8 páginas, 7 figuras, 1 tabla.-- PACS number(s): 75.30.Sg, 71.70.Ch, 75.30.Kz, 75.10.DgWe present a detailed study of the temperature dependence of the electrical resistivity [ρ(T)] in the range 13–300 K for the Ho5(SixGe1-x)4 system. Three distinct ρ(T) behaviors are observed, associated with different magnetic and crystallographic structures along the series. In the samples with an antiferromagnetic phase (AFM) one observes a shoulder near the Néel temperature (TN) attributed to the formation of a gap on the Fermi surface. This gap is analyzed using a phenomenological two-band model for an AFM with distinct atomic and magnetic periodicities, and its effect seems to extend well above TN. We also found the presence of short-range magnetic clusters in the paramagnetic (PM) phase. On the ferromagnetic (FM) materials, the distinct ρ(T) scattering contributions (phonon, magnetic, and residual terms) are extracted from the measurements, with ρ(T) mainly dominated by electron spin scattering. An additional contribution is also observed, arising from the strong crystal field effect in these materials. The effect is mainly observed in the PM phase, leading to a curvature on ρ(T) in this phase. Using a two-level crystal field model the corresponding gap was estimated for the different Si/Ge ratio samples, revealing that the crystal field splitting increases linearly with Si content.Work partially supported by the projects POCI/CTM/61284/2004, PTDC/CTM/NAN/115125/2009, and FEDER/POCTI n0155/94 from Fundaçâo para a Ciência ex Tecnologia (FCT), Portugal. A.M.P. thanks FCT for Grant No. SFRH/BPD/63150/2009. C.M. acknowledges the support of the Fundación ARAID. The financial support of the Spanish MEC (MAT2008-06567-C02) and DGA (Grant No. E26) is acknowledged.Peer reviewe

Demandas em arranjos agroflorestais com ênfase em fruteiras para dois territórios de identidade no Estado da Bahia.

Este trabalho tem como objetivo conhecer e avaliar problemas, apontar oportunidades que possam promover o desenvolvimento regional por meio de alternativas de produção, de inclusão social visando ao aumento de renda e a sustentabilidade da agricultura de base familiar, através da introdução de fruteiras e da cultura do nim indiano (Azadiracta indica), como fonte de energia, madeira e matéria-prima para controle de pragas na agropecuária. O Projeto abrange dois territórios de identidade, com características distintas, Recôncavo (Figura 1) e Bacia do Paramirim (Figura 2) com climas sub-úmido e semiárido respectivamente, e tem como objetivo transferir tecnologias apropriadas para a agricultura familiar, utilizando nim indiano consorciado com frutíferas arbóreas, culturas alimentares e forrageiras resultando em Sistemas Agroflorestais (SAFs). Por meio de ferramentas de Diagnóstico Rápido Participativo (DRP) possibilita-se o resgate, de forma rápida e concisa, da exploração agrícola de base familiar em ambos os Territórios, tornando os agricultores atores em todas as fases. A aplicação de DRPs junto à comunidade rural de base familiar exige a ordenação dos conhecimentos disponíveis referentes à problemática do espaço rural onde vive a comunidade de agricultores, bem como, o comprometimento e empenho na resolução dos problemas enfrentados no seu cotidiano. Os Sistemas Agroflorestais (SAFs) representam alternativas para a manutenção de uma agricultura de base familiar, face ao elevado potencial que apresentam para alcançar a sustentabilidade da atividade agropecuária. Os SAFs se constituem em sistemas de produção, do uso da terra de maneira racional, estratégica e tática, pois a produção é considerada por unidade de área, principalmente pela combinação simultânea ou escalonada de espécies anuais, semiperenes, perenes, madeireiras, não-madeireiras e de criações de animais, de forma compatível com os padrões dos agricultores familiares. As propriedades emergentes do SAF são chamadas de holismo, ou seja, o todo é mais do que a soma das partes. A sustentabilidade é uma característica inerente aos sistemas agroflorestais, pois estão alicerçados em princípios básicos que envolvem aspectos ecológicos, econômicos e sociais.pdf 259