Photosynthetic planulae and planktonic hydroids: contrasting strategies of propagule survival

Settlement delays can be important to prevent propagule waste when proper settling substrates are not immediately available. Under laboratory conditions, the planulae of Clytia viridicans underwent two alternative developmental patterns. Some settled on the bottom, forming a hydranth-gonotheca complex that produced up to four medusae and later either degenerated or gave rise to a hydroid colony. Other planulae settled right below the air-water interface, forming floating colonies that eventually fell to the bottom and settled. Halecium nanum released planulae with a rich population of symbiotic zooxanthellae that survived into a rearing jar for three months. After a long period of apparent quiescence (possibly fuelled by photosynthetic activities of zooxanthellae) the planulae produced new colonies. Both photosynthetic planulae and settlement at the interface air-water allow a delay in the passage from a planktonic to a fully functional benthic life

Probabilistic models for the erosion rate in embankments and reliability analysis of earth dams

Probabilistic models for the concentrated leak erosion of earthen water retaining structures are presented. The models predict the values of the critical shear stress, the coefficient of erosion and the pipe radius enlargement, starting from other measurable soil properties and the geometrical dimensions of the embankment. The models account for both the non-cohesive and cohesive contributions to the erosion behavior. A Bayesian approach is used for the treatment of the unknown parameters. An importance sampling simulation is adopted to calibrate the models and estimate the posterior distribution of the unknown model parameters using laboratory and in situ experimental data. The new proposed probabilistic model for the pipe radius is then used to develop fragility curves that capture the pipe enlargement as a function of time for a given earth dam

Late Summer Phytoplankton Blooms in the Changing Polar Environment of the Kongsfjorden (Svalbard, Arctic)

Kongsfjorden (Spitsbergen, Svalbard) is an inlet treated as a model site for studies on the impact of climate change in the Arctic due to its hydrological features. In this research, seven-days monitoring was carried out to evaluate the effects of hydrological variability on phytoplankton biomass and diversity in the late summer period. Temperature, salinity, nutrients, total suspended matter, phytoplankton abundance and biomass were determined for each sample. The thermo-haline properties of the column water seemed to affect phytoplankton communities. Their abundances and biomass were correlated with the amount of the total suspended matter. Moreover, species composition and biomass dramatically changed throughout the study period. Cold-water and Atlantic species were replaced by temperatewarm water dinoflagellates, including harmful species. An increase in phytoplankton biomass as well as the presence of dinoflagellate aggregations, mainly composed of Prorocentrum cf. gracile, were detected. This kind of algal accumulation is a new phenomenon in the Arctic and was probably related to the mobilization of sediment-rich glacial meltwaters. These findings, even if preliminary, suggest the need to study how additional biomass pulses and the increase of harmful species may alter the food web structure and the biogeochemical cycles, leading to major ecosystem changes

Is there Quark Matter in (Low-Mass) Pulsars?

The effect of the QCD phase transition is studied for the mass-radius relation of compact stars and for hot and dense matter at a given proton fraction used as input in core-collapse supernova simulations. The phase transitions to the 2SC and CFL color superconducting phases lead to stable hybrid star configurations with a pure quark matter core. In supernova explosions quark matter could be easily produced due to $\beta$-equilibrium, small proton fractions and nonvanishing temperatures. A low critical density for the phase transition to quark matter is compatible with present pulsar mass measurements.Comment: 4 pages, 3 figures, talk given at the QM2008 conference, Jaipur, India, February 4-10, 2008, JPG in pres

Bacterial ageing in the absence of external stressors

This is the final version. Available from the Royal Society via the DOI in this record.Evidence of ageing in the bacterium Escherichia coli was a landmark finding in senescence research, as it suggested that even organisms with morphologically symmetrical fission may have evolved strategies to permit damage accumulation. However, recent work has suggested that ageing is only detectable in this organism in the presence of extrinsic stressors, such as the fluorescent proteins and strong light sources typically used to excite them. Here we combine microfluidics with brightfield microscopy to provide evidence of ageing in E. coli in the absence of these stressors. We report (i) that the doubling time of the lineage of cells that consistently inherits the 'maternal old pole' progressively increases with successive rounds of cell division until it reaches an apparent asymptote, and (ii) that the parental cell divides asymmetrically, with the old pole daughter showing a longer doubling time and slower glucose accumulation than the new pole daughter. Notably, these patterns arise without the progressive accumulation or asymmetric partitioning of observable misfolded-protein aggregates, phenomena previously hypothesized to cause the ageing phenotype. Our findings suggest that ageing is part of the naturally occurring ecologically-relevant phenotype of this bacterium and highlight the importance of alternative mechanisms of damage accumulation in this context.Medical Research CouncilEPSRCBBSRCRoyal SocietyWellcome Trus

Production of benzaldehyde, benzyl alcohol and benzoic acid by yeasts and Botrytis cinerea isolated from grape musts and wines

The capacity of 100 yeast strains - isolated from grape musts and wines from the Istituto Sperimentale per l'Enologia collection - to produce benzaldehyde, benzyl alcohol and benzoic acid was verified by inoculation into a synthetic nutrient medium (MNS). Schizosaccharomyces and Zygosaccharomyces were strongest in producing benzaldehyde (maximal amount found 1200 µg/l) and benzyl alcohol (maximally 523 µg/l). Zygosaccharomyces was also most effective in the production of benzoic acid (maximally 536 µg/l), followed by Saccharomyces, Cryptococcus, Kloeckera and Torulaspora. The hypothesis was verified that yeasts can be an exogenous source of the benzyl alcohol oxidizing enzyme in grape musts and wines. Wine yeast strains of Saccharomyces spp., Zygosaccharomyces spp. and Schizosaccharomyces spp. fermenting MNS containing 150 g/l glucose, with benzyl alcohol added, transformed this into benzoic acid only when glucose was disappearing, but not into benzaldehyde. No difference was observed between aerobic and anaerobic fermentation conditions. The uptake of benzyl alcohol was rapid in fermentation essays in presence of only 10 g/l glucose and in assimilation essais performed in yeast nitrogen base broth with assimilable carbon compounds added. A catabolic repression by glucose appears likely. Botrytis cinerea was able to transform benzyl alcohol into benzaldehyde and benzoic acid on Czapek-Dox broth with 30 g/l sucrose added. Benzyl alcohol was transformed by wine yeasts into benzoic acid when the concentration of glucose in the mineral medium was less than 10 g/l, but no production of benzaldehyde was observed. A catabolic repression of this transformation by glucose is likely. Botrytis cinerea was able to produce benzaldehyde in a mineral medium with benzyl alcohol and sucrose added

Bioactive potential of two marine picocyanobacteria belonging to Cyanobium and Synechococcus genera

Coccoid cyanobacteria produce a great variety of secondary metabolites, which may have useful properties, such as antibacterial, antiviral, anticoagulant or anticancer activities. These cyanobacterial metabolites have high ecological significance, and they could be considered responsible for the widespread occurrence of these microorganisms. Considering the great benefit derived from the identification of competent cyanobacteria for the extraction of bioactive compounds, two strains of picocyanobacteria (coccoid cyanobacteria < 3 µm) (Cyanobium sp. ITAC108 and Synechococ-cus sp. ITAC107) isolated from the Mediterranean sponge Petrosia ficiformis were analyzed. The biological effects of organic and aqueous extracts from these picocyanobacteria toward the nauplii of Artemia salina, sea urchin embryos and human cancer lines (HeLa cells) were evaluated. Methanolic and aqueous extracts from the two strains strongly inhibited larval development; on the contrary, in ethyl acetate and hexane extracts, the percentage of anomalous embryos was low. Moreover, all the extracts of the two strains inhibited HeLa cell proliferation, but methanol extracts exerted the highest activity. Gas chromatography–mass spectrometry analysis evidenced for the first time the presence of β-N-methylamino-L-alanine and microcystin in these picocyanobacteria. The strong cytotoxic activity observed for aqueous and methanolic extracts of these two cyanobacteria laid the foundation for the production of bioactive compounds of pharmacological interest

Strange quark matter in explosive astrophysical systems

Explosive astrophysical systems, such as supernovae or compact star binary mergers, provide conditions where strange quark matter can appear. The high degree of isospin asymmetry and temperatures of several MeV in such systems may cause a transition to the quark phase already around saturation density. Observable signals from the appearance of quark matter can be predicted and studied in astrophysical simulations. As input in such simulations, an equation of state with an integrated quark matter phase transition for a large temperature, density and proton fraction range is required. Additionally, restrictions from heavy ion data and pulsar observation must be considered. In this work we present such an approach. We implement a quark matter phase transition in a hadronic equation of state widely used for astrophysical simulations and discuss its compatibility with heavy ion collisions and pulsar data. Furthermore, we review the recently studied implications of the QCD phase transition during the early post-bounce evolution of core-collapse supernovae and introduce the effects from strong interactions to increase the maximum mass of hybrid stars. In the MIT bag model, together with the strange quark mass and the bag constant, the strong coupling constant $\alpha_s$ provides a parameter to set the beginning and extension of the quark phase and with this the mass and radius of hybrid stars.Comment: 6 pages, 5 figures, talk given at the International Conference on Strangeness in Quark Matter (SQM2009), Buzios, Brasil, September 28 - October 2, 2009, to be published in Journal Phys.

Flavor Symmetry and Topology Change in Nuclear Symmetry Energy for Compact Stars

The nuclear symmetry energy figures crucially in the structure of asymmetric nuclei and, more importantly, in the equation of state (EoS) of compact stars. At present it is almost totally unknown, both experimentally and theoretically, in the density regime appropriate for the interior of neutron stars. Basing on a strong-coupled structure of dense baryonic matter encoded in the skyrmion crystal approach with a topology change and resorting to the notion of generalized HLS (hidden local symmetry) in hadronic interactions, we address a variety of hitherto unexplored issues of nuclear interactions associated with the symmetry energy, i.e., kaon condensation and hyperons, possible topology change in dense matter, nuclear tensor forces, conformal symmetry and chiral symmetry etc in the EoS of dense compact-star matter. One of the surprising results coming from the hidden local symmetry structure that is distinct from what is given by standard phenomenological approaches is that at high density, baryonic matter is driven by RG flow to the "dilaton-limit fixed point (DLFP)" constrained by "mended symmetries." We further propose how to formulate kaon condensation and hyperons in compact-star matter in a framework anchored on a single effective Lagrangian by treating hyperons as the Callan-Klebanov kaon-skyrmion bound states simulated on crystal lattice. This formulation suggests that hyperons can figure in the stellar matter -- if at all -- when or after kaons condense, in contrast to the standard phenomenological approaches where the hyperons appear as the first strangeness degree of freedom in matter thereby suppressing or delaying kaon condensation. In our simplified description of the stellar structure in terms of symmetry energies which is compatible with that of the 1.97 solar mass star, kaon condensation plays a role of "doorway state" to strange-quark matter.Comment: 48 pages, 6 figures, version for publicatio

Strangeness in Astrophysics and Cosmology

Some recent developments concerning the role of strange quark matter for astrophysical systems and the QCD phase transition in the early universe are addressed. Causality constraints of the soft nuclear equation of state as extracted from subthreshold kaon production in heavy-ion collisions are used to derive an upper mass limit for compact stars. The interplay between the viscosity of strange quark matter and the gravitational wave emission from rotation-powered pulsars are outlined. The flux of strange quark matter nuggets in cosmic rays is put in perspective with a detailed numerical investigation of the merger of two strange stars. Finally, we discuss a novel scenario for the QCD phase transition in the early universe, which allows for a small inflationary period due to a pronounced first order phase transition at large baryochemical potential.Comment: 8 pages, invited talk given at the International Conference on Strangeness in Quark Matter (SQM2009), Buzios, Brasil, September 28 - October 2, 200