The Suzaku Observations of SS Cygni in Quiescence and Outburst

We present results from the Suzaku observations of the dwarf nova SS Cyg in quiescence and outburst in 2005 November. Owing to high sensitivity of the HXD PIN detector and high spectral resolution of the XIS, we have determined parameters of the plasma with unprecedented precision. The maximum temperature of the plasma in quiescence 20.4 +4.0-2.6 (stat.) +/- 3.0 (sys.) keV is significantly higher than that in outburst 6.0 +0.2-1.3 keV. The elemental abundances are close to the solar ones for the medium-Z elements (Si, S, Ar) whereas they decline both in lighter and heavier elements. Those of oxygen and iron are 0.46 and 0.37 solar, respectively. That of carbon is exceptionally high and 2 solar at least. The solid angle of the reflector subtending over the optically thin thermal plasma is Omega/2\pi = 1.7+/-0.2 (stat.) +/-0.1 (sys.) in quiescence. A 6.4 keV iron Ka line is resolved into a narrow and broad components. These facts indicate that both the white dwarf and the accretion disk contribute to the continuum reflection and the 6.4 keV iron Ka line. We consider the standard optically thin boundary layer as the most plausible picture for the plasma configuration in quiescence. The solid angle of the reflector in outburst Omega/2\pi = 0.9 +0.5-0.4 and a broad 6.4 keV iron line indicates that the reflection in outburst originates from the accretion disk and an equatorial accretion belt. From the energy width of the 6.4 keV line, we consider the optically thin thermal plasma in outburst as being distributed on the accretion disk like solar coronae.Comment: 28 pages, 15 figures, accepted for publication in PASJ Suzaku 3rd special issue Pdf of this paper can be downloaded from http://www.astro.isas.jaxa.jp/~ishida/Papers/sscyg_sub2.pd

Leiomyosarcoma of the sigmoid colon with multiple liver metastases and gastric cancer: a case report

BACKGROUND: Leiomyosarcoma (LMS) of the gastrointestinal tract is an extremely rare high-grade neoplasm with poor prognosis. For advanced LMS with distant metastasis, the decision as to the choice of the most appropriate therapeutic strategy, including chemotherapy and surgery, is difficult. Here, we present an unusual case of LMS of the sigmoid colon with liver metastases and gastric cancer. The survival of this patient was prolonged by a combined modality therapy involving chemotherapy and surgery. CASE PRESENTATION: A 66-year-old woman who had been diagnosed with advanced gastric cancer and multiple liver metastases was referred to our hospital. The initial treatment with docetaxel and S-1 considerably reduced both the gastric cancer and liver tumors; consequently we performed surgical resection. Pathological examination revealed that no viable tumor cells remained in the stomach and chemotherapy resulted in complete remission of the gastric cancer. The liver tumors were immunohistochemically diagnosed as LMS. A tumor of the sigmoid colon was subsequently discovered and the liver tumors were found to have recurred. The surgically resected sigmoid colon and liver tumors were all immunohistochemically diagnosed as LMS. These findings indicated that the multiple liver metastases arose from the LMS in the sigmoid colon, and that they were accompanied by advanced gastric cancer. We performed another surgical resection and administered chemotherapy to treat the recurring liver metastases. The patient survived for 4 years and 10 months after initial presentation at our hospital. CONCLUSION: Colonic LMS is rare and its joint occurrence with gastric cancer is extremely unusual. Although LMS is a high-grade neoplasm, a multimodal therapeutic approach can increase patient survival time even when multiple liver metastases are present

Comparative Study of Human Hematopoietic Cell Engraftment into Balb/c and C57BL/6 Strain of Rag-2/Jak3 Double-Deficient Mice

Immunodeficient mice are becoming invaluable tools in human stem cell and tumor research. In this study, we generated Rag-2/Jak3 double-deficient (Rag-2−/−Jak3−/−) mice with a C57/BL6 and Balb/c genetic background and compared the human lymphohematopoietic cell engraftment rate. Human cord blood-derived CD34+ hematopoietic stem cells were successfully engrafted into Balb/c Rag-2−/−Jak3−/− mice; however, the engraftment rate was far lower in C57/BL6 Rag-2−/−Jak3−/− mice. Transplantation of human peripheral blood mononuclear cells resulted in the same tendency. Thus, a Balb/c background offers superior engraftment capacity than a C57/BL6 background and provides an attractive model for human hematopoietic cell engraftment

Work-up, diagnosis and management of acute Vogt-Koyanagi-Harada disease: A case of acute myopization with granulomatous uveitis

Purpose: In its typical form and when seen at onset, Vogt-Koyanagi-Harada (VKH) is characterized by easily recognizable signs that allow diagnosis without difficulty. In cases that do not have acute onset, that are seen at a later stage or that do not show the complete set of signs, appraisal is more difficult and diagnosis may cause difficulties. We present here a case of bilateral granulomatous uveitis compatible with VKH disease in order to allow several experts to give their opinion on the most appropriate manner to confirm or reject the diagnosis and their approach to the management of the case. Case presentation: A 17-year-old female patient consulted her ophthalmologist for blurred vision OU following an episode of a flu-like disease with malaise, fever and headaches. A bilateral anterior granulomatous uveitis with a right papillitis was diagnosed and the patient presented with a bilateral acute myopization. Fluorescein angiography showed right disc hyperfluorescence with late leakage and slight left disc hyperfluorescence. The patient was given a course of one week of peroral corticosteroid therapy followed by an intramuscular injection of Bentelan® twice weekly. In the absence of significant improvement the patient was sent sixweeks later to a specialized center where a complete work-up was performed. Expert opinion: The diagnostic work-up, investigational tests, and differential diagnosis to confirm or reject the diagnosis of VKH as well as the management of the case will be described by the expert

Vaccination with EphA2-derived T cell-epitopes promotes immunity against both EphA2-expressing and EphA2-negative tumors

BACKGROUND: A novel tyrosine kinase receptor EphA2 is expressed at high levels in advanced and metastatic cancers. We examined whether vaccinations with synthetic mouse EphA2 (mEphA2)-derived peptides that serve as T cell epitopes could induce protective and therapeutic anti-tumor immunity. METHODS: C57BL/6 mice received subcutaneous (s.c.) vaccinations with bone marrow-derived dendritic cells (DCs) pulsed with synthetic peptides recognized by CD8+ (mEphA2(671–679), mEphA2(682–689)) and CD4+ (mEphA2(30–44)) T cells. Splenocytes (SPCs) were harvested from primed mice to assess the induction of cytotoxic T lymphocyte (CTL) responses against syngeneic glioma, sarcoma and melanoma cell lines. The ability of these vaccines to prevent or treat tumor (s.c. injected MCA205 sarcoma or B16 melanoma; i.v. injected B16-BL6) establishment/progression was then assessed. RESULTS: Immunization of C57BL/6 mice with mEphA2-derived peptides induced specific CTL responses in SPCs. Vaccination with mEPhA2 peptides, but not control ovalbumin (OVA) peptides, prevented the establishment or prevented the growth of EphA2+ or EphA2-negative syngeneic tumors in both s.c. and lung metastasis models. CONCLUSIONS: These data indicate that mEphA2 can serve as an attractive target against which to direct anti-tumor immunity. The ability of mEphA2 vaccines to impact EphA2-negative tumors such as the B16 melanoma may suggest that such beneficial immunity may be directed against alternative EphA2+ target cells, such as the tumor-associated vascular endothelial cells

NOS2 polymorphisms associated with the susceptibility to pulmonary arterial hypertension with systemic sclerosis: contribution to the transcriptional activity

Systemic sclerosis (SSc) is a connective tissue disease characterized by tissue fibrosis. One of several complications of SSc, pulmonary arterial hypertension (PAH) can be refractory to treatment, both novel and established. In the present study we investigated the ratio of circulating nitric oxide to endothelin-1 in patients with both SSc and PAH, and determined whether polymorphisms in NOS2 (the nitric oxide synthase 2 gene) are associated with susceptibility to PAH. Endothelin-1 in plasma and nitric oxide metabolites (nitrate and nitrite) in serum were measured. The nitric oxide/endothelin-1 ratio was significantly lower in patients with both SSc and PAH than in patients with SSc only or in healthy control individuals. We confirmed the presence of two single nucleotide polymorphisms at positions -1,026 and -277 and a pentanucleotide repeat (CCTTT) at -2.5 kilobases. There were significant differences in single nucleotide polymorphisms between patients with SSc who had PAH and those who did not, and between patients with both SSc and PAH and healthy control individuals. The CCTTT repeat was significantly shorter in patients with both SSc and PAH than in patients with SSc only or in healthy control individuals. Transcriptional activity were analyzed using the luciferase reporter assay. The transcriptional activity of NOS2 was much greater in fibroblasts transfected by a vector with a long allele of the CCTTT repeat than in those transfected by a vector with a short allele. Polymorphisms in the NOS2 gene are associated with transcriptional activity of the NOS2 gene and with susceptibility to SSc-related PAH

Suzaku Discovery of Hard X-ray Pulsations from the Rotating Magnetized White Dwarf, AE Aquarii

The fast rotating magnetized white dwarf, AE Aquarii, was observed with Suzaku, in October 2005 and October 2006 with exposures of 53.1 and 42.4 ks, respectively. In addition to clear spin modulation in the 0.5--10 keV band of the XIS data at the barycentric period of 33.0769 \pm 0.0001 s, the 10--30 keV HXD data in the second half of the 2005 observation also showed statistically significant periodic signals at a consistent period. On that occasion, the spin-folded HXD light curve exhibited two sharp spikes separated by about 0.2 cycles in phase, in contrast to approximately sinusoidal profiles observed in energies below about 4 keV. The folded 4--10 keV XIS light curves are understood as a superposition of those two types of pulse profiles. The phase averaged 1.5--10 keV spectra can be reproduced by two thermal components with temperatures of $2.90_{-0.16}^{+0.20}$ keV and $0.53_{-0.13}^{+0.14}$ keV, but the 12-25 keV HXD data show a significant excess above the extrapolated model. This excess can be explained by either a power-law model with photon index of $1.12_{-0.62}^{+0.63}$ or a third thermal component with a temperature of $54_{-47}^{+26}$ keV. At a distance of 102 pc, the 4--30 keV luminosities of the thermal and the additional components become $1.7_{-0.6}^{+1.3}$ and $5.3_{-0.3}^{+15.3} \times 10^{29}$ erg s$^{-1}$, respectively. The latter corresponds to 0.09% of the spin down energy of the object. Possible emission mechanisms of the hard pulsations are discussed, including in particular non-thermal ones.Comment: Accepted for publication on PASJ Vol.60, No.2, 2008 see Press Release page(http://www.heal.phy.saitama-u.ac.jp/~terada/01work/press_release2008/index_e.html

DLPFC and KYN in MDD treatment response

Aim: To establish treatment response biomarkers that reflect the pathophysiology of depression, it is important to use an integrated set of features. This study aimed to determine the relationship between regional brain activity at rest and blood metabolites related to treatment response to escitalopram to identify the characteristics of depression that respond to treatment. Methods: Blood metabolite levels and resting-state brain activity were measured in patients with moderate to severe depression (n = 65) before and after 6–8 weeks of treatment with escitalopram, and these were compared between Responders and Nonresponders to treatment. We then examined the relationship between blood metabolites and brain activity related to treatment responsiveness in patients and healthy controls (n = 36). Results: Thirty-two patients (49.2%) showed a clinical response (>50% reduction in the Hamilton Rating Scale for Depression score) and were classified as Responders, and the remaining 33 patients were classified as Nonresponders. The pretreatment fractional amplitude of low-frequency fluctuation (fALFF) value of the left dorsolateral prefrontal cortex (DLPFC) and plasma kynurenine levels were lower in Responders, and the rate of increase of both after treatment was correlated with an improvement in symptoms. Moreover, the fALFF value of the left DLPFC was significantly correlated with plasma kynurenine levels in pretreatment patients with depression and healthy controls. Conclusion: Decreased resting-state regional activity of the left DLPFC and decreased plasma kynurenine levels may predict treatment response to escitalopram, suggesting that it may be involved in the pathophysiology of major depressive disorder in response to escitalopram treatment