Analisis Unit Cost Pelayanan Rawat Inap Postpartum Di Rumah Sakit Umum Dewi Sartika Dengan Menggunakan Metode Activity Based Costing (ABC) System

Iklim kompetitif tidak hanya terjadi pada Perusahaan yang berorientasi profit, namun juga berdampak padaperusahaan yang berorientasi nonprofit, salah satunya adalah rumah sakit. Rumah sakit yang berada di SulawesiTenggara pada tahun 2014 berjumlah 36, sedangkan kota Kendari memiliki 13 rumah sakit dan harus melayani347.496 penduduk. Hal ini menjadikan persaingan bisnis rumah sakit baik umum maupun swasta kini semakinketat. Salah satu solusi untuk memenangkan persaingan adalah dengan cara menentukan tarif yang lebih rendahdan kualitas atau jasa yang lebih tinggi dari pada pesaing. Solusi tersebut tentunya menjadikan biaya operasionalrumah sakit akan semakin besar sehingga sistem akuntansi manajemen di rumah sakit harus efektif dan efisiensehingga menghasilkan informasi yang akurat dalam pengambilan keputusan. Rumah sakit cenderung masihmenggunakan system akuntansi tradisional yang memiliki distorsi biaya. Penelitian ini menganalisis perhitungantarif rawat inap pelayanan postpartum di RSU Dewi Sartika menggunakan metode activity based costing system.Penelitian ini menggunakan metode deskriptif komparatif dengan pendekatan kuantitatif, data yang digunakanadalah seluruh data keuangan pada Oktober 2015-September 2016 dan aktivitas yang diobservasi yaitu ruangperawatan kelas VIP, kelas I, kelas II, kelas III dan bangsal. Hasil perhitungan menunjukkan hasil yang berbeda daritarif yang diterapkan. Tarif kelas VIP, kelas I dan kelas III lebih rendah dari tarif rumah sakit sedangkan tarif kelas IIdan bangsal lebih tinggi

Mining for nonribosomal peptide synthetase and polyketide synthase genes revealed a high level of diversity in the sphagnum bog metagenome

Sphagnum bog ecosystems are one of the oldest vegetation forms harbouring a specific microbial community, which is known to produce an exceptionally wide variety of bioactive substances. Although the Sphagnum metagenome indicate a rich secondary metabolism, the genes are not yet explored. To analyse non-ribosomal peptide synthetases (NRPS) and polyketide synthases (PKS) the diversity of NRPS and PKS genes in Sphagnum-associated metagenome was investigated by in silico data mining and sequence-based screening (PCR-amplification of 9500 fosmid clones). The in silico Illumina-based metagenomic approach resulted in the identification of 279 NRPS, 346 PKS, as well as 40 PKS-NRPS hybrid gene sequences. Occurrence of NRPS sequences was strongly dominated by the phyla Protebacteria, especially by the genus Burkholderia, while PKS sequences were mainly affiliated to Actinobacteria. Thirteen novel NRPS-related sequences were identified by PCR-amplification screening, displaying amino acid sequence identities of 48 to 91% to annotated sequences of the phyla Proteobacteria, Actinobacteria and Cyanobacteria. Some of the identified metagenomic clones showed closest similarity to peptide synthases from Burkholderia or Lysobacter, which are emerging bacterial sources of yet undescribed bioactive metabolites. This study highlights the role of the extreme natural ecosystems as a promising source for detection of secondary compounds and enzymes, serving as a source for biotechnological applications

Data from: The Sphagnum microbiome supports greatly bog ecosystem functioning under extreme conditions

Sphagnum-dominated bogs represent a unique yet widely distributed type of terrestrial ecosystem, and strongly contribute to global biosphere functioning. Sphagnum is colonised by highly diverse microbial communities, but less is known about their function. We identified a high functional diversity within the Sphagnum microbiome applying an Illumina-based metagenomic approach followed by de novo assembly and MG-RAST annotation. An inter-environmental comparison revealed that the Sphagnum microbiome harbours specific genetic features that distinguish it significantly from microbiomes of higher plants and peat soils. The differential traits especially support ecosystem functioning by a symbiotic life style under poikilohydric and ombrotrophic conditions. To realise a plasticity-stability balance, we found abundant subsystems responsible to cope with oxidative and drought stresses, to exchange (mobile) genetic elements, and genes that encode for resistance to detrimental environmental factors, repair, and self-controlling mechanisms. Multiple microbe-microbe and plant-microbe interactions were also found to play a crucial role as indicated by diverse genes necessary for biofilm formation, interaction via quorum sensing and nutrient exchange. A high proportion of genes involved in nitrogen cycle and recycling of organic material supported the role of bacteria for nutrient supply. 16S rDNA analysis indicated a higher structural diversity than that which had been previously detected using PCR-dependent techniques. Altogether, the diverse Sphagnum microbiome has the ability to support the life of the host plant and the entire ecosystem under changing environmental conditions. Beyond this, the moss microbiome presents a promising bio-resource for environmental biotechnology – with respect to novel enzymes or stress-protecting bacteria

Data from: Mining for NRPS and PKS genes revealed a high diversity in the Sphagnum bog metagenome

Sphagnum bog ecosystems are among the oldest vegetation forms harboring a specific microbial community and are known to produce an exceptionally wide variety of bioactive substances. Although the Sphagnum metagenome shows a rich secondary metabolism, the genes have not yet been explored. To analyze nonribosomal peptide synthetases (NRPSs) and polyketide synthases (PKSs), the diversity of NRPS and PKS genes in Sphagnum-associated metagenomes was investigated by in silico data mining and sequence-based screening (PCR amplification of 9,500 fosmid clones). The in silico Illumina-based metagenomic approach resulted in the identification of 279 NRPSs and 346 PKSs, as well as 40 PKS-NRPS hybrid gene sequences. The occurrence of NRPS sequences was strongly dominated by the members of the Protebacteria phylum, especially by species of the Burkholderia genus, while PKS sequences were mainly affiliated with Actinobacteria. Thirteen novel NRPS-related sequences were identified by PCR amplification screening, displaying amino acid identities of 48% to 91% to annotated sequences of members of the phyla Proteobacteria, Actinobacteria, and Cyanobacteria. Some of the identified metagenomic clones showed the closest similarity to peptide synthases from Burkholderia or Lysobacter, which are emerging bacterial sources of as-yet-undescribed bioactive metabolites. This report highlights the role of the extreme natural ecosystems as a promising source for detection of secondary compounds and enzymes, serving as a source for biotechnological applications

4516652.3_M2norm_L008_R1_paired_de_novo_assembly_Nmetabolism

Partial gene sequences encoding for nitrogen metabolism from the normalised Sphagnum magellanicum metagenome

4533611.3_M2_retainMode_16S

Partial 16S rRNA gene sequences from the complete Sphagnum magellanicum metagenom

4533611.3_M2_retianMode_Nmetabolism

Partial gene sequences encoding for nitrogen metabolism from the complete Sphagnum magellanicum metagenome

NRPS-PKS hybrid sequences from the Sphagnum magellanicum assembled metagenome

NRPS-PKS hybrid sequences from the Sphagnum magellanicum assembled metagenom