144 research outputs found
Penicillium crustosum as a potential OTA producer - new insights from whole - genome sequencing of strain MUM 16.125
Ochratoxin A (OTA) is a well-studied mycotoxin that poses severe health risks. OTA is mainly
produced by Aspergillus and Penicillium species associated with food spoilage and it is present
in a wide diversity of food and feed products. Recent studies have reported the presence of
OTA in food matrices where known OTA producers are not present1,2. For that reason, other
species such as P. crustosum are now being considered. A recent study using comparative
genomic analysis3 clarified the OTA biosynthetic gene cluster composition.
In order to gain insight into the secondary metabolism of P. crustosum, this study aimed to
sequence and explore the complete genome of strain MUM 16.125. This strain was isolated
from cheese rind sample contaminated with OTA in which no known OTA producers were
present1.
The genome assembly comprises 199 contigs with a total length of 30.95 Mb and contains
10975 predicted protein-coding genes. In total, 109 gene clusters potentially related with
secondary metabolism were identified, including putative gene clusters for penitrem, clavaric
acid or naphthopyrones biosynthesis. Nevertheless, no evidence of an OTA biosynthetic gene
cluster was found. A total of 83 complete and 49 partial protein sequences from published OTA
biosynthetic genes from 11 Aspergillus and 3 Penicillium species were queried against the
predicted P. crustosum proteins. Only 3 strong matches were found (to a short partial P.
verrucosum PKS and 2 P. thymicola chloroperoxidases) but matches to complete key genes
were absent.
Considering these findings, it appears that strain MUM 16.125 lacks the most common genetic
pathway to produce OTA, providing important information relevant to understand the role of P.
crustosum as putative OTA producer. Nevertheless, the additional secondary metabolism gene
clusters found (such as penitrem, clavaric acid or naphthopyrones) highlight the potential of
this strain for metabolite production, including other mycotoxins or compounds with antioxidant,
anticancer or antibiotic properties.This study was supported by the Portuguese Foundation for Science and Technology
(FCT) under the scope of the strategic funding of CEB (UID/BIO/04469/2019) and iBiMED (UIDB/04501/2020)
units; and by CANCYL (POCI-01-0145-FEDER-031849) and GenomePT (POCI-01-0145-FEDER-022184)
projectsinfo:eu-repo/semantics/publishedVersio
Dre-miR-2188 Targets Nrp2a and Mediates Proper Intersegmental Vessel Development in Zebrafish Embryos
BACKGROUND: MicroRNAs (miRNAs) are a class of small RNAs that are implicated in the control of eukaryotic gene expression by binding to the 3'UTR of target mRNAs. Several algorithms have been developed for miRNA target prediction however, experimental validation is still essential for the correct identification of miRNA targets. We have recently predicted that Neuropilin2a (Nrp2a), a vascular endothelial growth factor receptor which is essential for normal developmental angiogenesis in zebrafish, is a dre-miR-2188 target. METHODOLOGY: Here we show that dre-miR-2188 targets the 3'-untranslated region (3'UTR) of Nrp2a mRNA and is implicated in proper intersegmental vessel development in vivo. Over expression of miR-2188 in zebrafish embryos down regulates Nrp2a expression and results in intersegmental vessel disruption, while its silencing increases Nrp2a expression and intersegmental vessel sprouting. An in vivo GFP sensor assay based on a fusion between the GFP coding region and the Nrp2a 3'UTR confirms that miR-2188 binds to the 3'UTR of Nrp2a and inhibits protein translation. CONCLUSIONS: We demonstrate that miR-2188 targets Nrp2a and affects intersegmental vessel development in zebrafish embryos
Large Scale Comparative Codon-Pair Context Analysis Unveils General Rules that Fine-Tune Evolution of mRNA Primary Structure
BACKGROUND: Codon usage and codon-pair context are important gene primary structure features that influence mRNA decoding fidelity. In order to identify general rules that shape codon-pair context and minimize mRNA decoding error, we have carried out a large scale comparative codon-pair context analysis of 119 fully sequenced genomes. METHODOLOGIES/PRINCIPAL FINDINGS: We have developed mathematical and software tools for large scale comparative codon-pair context analysis. These methodologies unveiled general and species specific codon-pair context rules that govern evolution of mRNAs in the 3 domains of life. We show that evolution of bacterial and archeal mRNA primary structure is mainly dependent on constraints imposed by the translational machinery, while in eukaryotes DNA methylation and tri-nucleotide repeats impose strong biases on codon-pair context. CONCLUSIONS: The data highlight fundamental differences between prokaryotic and eukaryotic mRNA decoding rules, which are partially independent of codon usage
Genomic history of coastal societies from eastern South America
Sambaqui (shellmound) societies are among the most intriguing archaeological phenomena in pre-colonial South America, extending from approximately 8,000 to 1,000 years before present (yr bp) across 3,000 km on the Atlantic coast. However, little is known about their connection to early Holocene hunter-gatherers, how this may have contributed to different historical pathways and the processes through which late Holocene ceramists came to rule the coast shortly before European contact. To contribute to our understanding of the population history of indigenous societies on the eastern coast of South America, we produced genome-wide data from 34 ancient individuals as early as 10,000 yr bp from four different regions in Brazil. Early Holocene hunter-gatherers were found to lack shared genetic drift among themselves and with later populations from eastern South America, suggesting that they derived from a common radiation and did not contribute substantially to later coastal groups. Our analyses show genetic heterogeneity among contemporaneous Sambaqui groups from the southeastern and southern Brazilian coast, contrary to the similarity expressed in the archaeological record. The complex history of intercultural contact between inland horticulturists and coastal populations becomes genetically evident during the final horizon of Sambaqui societies, from around 2,200 yr bp, corroborating evidence of cultural change
Expression variability of co-regulated genes differentiates Saccharomyces cerevisiae strains
Background: Saccharomyces cerevisiae (Baker’s yeast) is found in diverse ecological niches and is characterized by
high adaptive potential under challenging environments. In spite of recent advances on the study of yeast
genome diversity, little is known about the underlying gene expression plasticity. In order to shed new light onto
this biological question, we have compared transcriptome profiles of five environmental isolates, clinical and
laboratorial strains at different time points of fermentation in synthetic must medium, during exponential and
stationary growth phases.
Results: Our data unveiled diversity in both intensity and timing of gene expression. Genes involved in glucose
metabolism and in the stress response elicited during fermentation were among the most variable. This gene
expression diversity increased at the onset of stationary phase (diauxic shift). Environmental isolates showed lower
average transcript abundance of genes involved in the stress response, assimilation of nitrogen and vitamins, and
sulphur metabolism, than other strains. Nitrogen metabolism genes showed significant variation in expression
among the environmental isolates.
Conclusions: Wild type yeast strains respond differentially to the stress imposed by nutrient depletion, ethanol
accumulation and cell density increase, during fermentation of glucose in synthetic must medium. Our results
support previous data showing that gene expression variability is a source of phenotypic diversity among closely
related organisms.Fundação para a Ciência e TecnologiaThe authors wish to thank Adega Cooperativa da Bairrada, Cantanhede,
Portugal, for providing the commercial strains
A produção escrita de alunos com e sem síndrome de Down: uma análise da coerência textual
Pervasive gaps in Amazonian ecological research
Biodiversity loss is one of the main challenges of our time,1,2 and attempts to address it require a clear un derstanding of how ecological communities respond to environmental change across time and space.3,4
While the increasing availability of global databases on ecological communities has advanced our knowledge
of biodiversity sensitivity to environmental changes,5–7 vast areas of the tropics remain understudied.8–11 In
the American tropics, Amazonia stands out as the world’s most diverse rainforest and the primary source of
Neotropical biodiversity,12 but it remains among the least known forests in America and is often underrepre sented in biodiversity databases.13–15 To worsen this situation, human-induced modifications16,17 may elim inate pieces of the Amazon’s biodiversity puzzle before we can use them to understand how ecological com munities are responding. To increase generalization and applicability of biodiversity knowledge,18,19 it is thus
crucial to reduce biases in ecological research, particularly in regions projected to face the most pronounced
environmental changes. We integrate ecological community metadata of 7,694 sampling sites for multiple or ganism groups in a machine learning model framework to map the research probability across the Brazilian
Amazonia, while identifying the region’s vulnerability to environmental change. 15%–18% of the most ne glected areas in ecological research are expected to experience severe climate or land use changes by
2050. This means that unless we take immediate action, we will not be able to establish their current status,
much less monitor how it is changing and what is being lostinfo:eu-repo/semantics/publishedVersio
A new cross-check and review of aerosol attenuation measurements at the Pierre Auger Observatory
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