Effects of Hippotherapy on Coordination of Speech in a Person with Traumatic Brain Injury

Hippotherapy occurs when physical therapists, occupational therapists, and speechlanguage pathologists use the movement of a horse as a treatment strategy. Previous research has documented improvements in gross motor function such as walking, reaching, standing, balance, and trunk control following treatment that incorporates hippotherapy. However, no study to date has investigated the effect of hippotherapy on fine motor control functions such as speech. The purpose of the study was to complete a pilot investigation of the effects of hippotherapy on speech motor control in one person with traumatic brain injury. The treatment records of a 24 yearold woman with TBI who received speech therapy using hippotherapy were reviewed and the speech motor control data from ten sessions was extrapolated. Results indicated immediate improvement in speech motor control with continued improvement through session ten

Modeling \u3ci\u3eEscherichia coli\u3c/i\u3e in the Missouri River near Omaha, Nebraska, 2012–16

The city of Omaha, Nebraska, has a combined sewer system in some areas of the city. In Omaha, Nebr., a moderate amount of rainfall will lead to the combination of stormwater and untreated sewage or wastewater being discharged directly into the Missouri River and Papillion Creek and is called a combined sewer overflow (CSO) event. In 2009, the city of Omaha began the implementation of their Long Term Control Plan (LTCP) to mitigate the effects of CSOs on the Missouri River and Papillion Creek. As part of the LTCP, the city partnered with the U.S. Geological Survey (USGS) in 2012 to begin monitoring in the Missouri River. Since 2012, monthly discrete water-quality samples for many constituents have been collected from the Missouri River at four sites. At 3 of the 4 sites, water quality has been monitored continuously for selected constituents and physical properties. These discrete water-quality samples and continuous water-quality monitoring data (from July 2012 to 2020) have been collected to better understand the water quality of the Missouri River, how it is changing with time, how it changes upstream from the city of Omaha to downstream, and how it varies during base-flow conditions and during periods of runoff. The purpose of this report is to document the development of Escherichia coli (E. coli) concentration models for these four Missouri River sites. Analysis was completed using the first 5 years of data (through 2016) to determine if the current approach is sufficient to meet future analysis goals and to understand if proposed models such as Load Estimator (LOADEST) models will be able to represent water-quality changes in the Missouri River. Multiple linear regression models were developed to estimate E. coli concentration using LOADEST as implemented in the rloadest package in the R statistical software program. A set of explanatory variables, including streamflow and streamflow anomalies, precipitation, information about CSOs, and continuous water quality, were evaluated for potential inclusion in regression models. The best model at Missouri River at NP Dodge Park at Omaha, Nebr. (USGS station 412126095565201; hereafter “NP Dodge”) included basin explanatory variables of upstream antecedent precipitation index measured at Tekamah, Nebr.; decimal time; season; and turbidity. The best model at Missouri River at Freedom Park Omaha, Nebr. (USGS station 411636095535401; hereafter “Freedom Park”) included the same explanatory variables as the NP Dodge model with the addition of turbidity anomalies and flow anomalies. The best models at the two downstream sites (Missouri River near Council Bluffs, Iowa, USGS station 06610505 and Missouri River near La Platte, Nebr., USGS station 410333095530101) included the same explanatory variables as the Freedom Park model with the addition of local antecedent precipitation index as measured at Eppley Airport in Omaha, Nebr., and additional turbidity and flow anomalies. The final selected models were the best models given our modeling design constraint in which explanatory variables included in the model for the upstream site were included in the downstream models. Explanatory variables currently (2020) being collected and included in the selected models through 2016 explained 64–75 percent of the variability of E. coli concentration in the Missouri River. Explaining 64–75 percent of the variability might be considered low when working with physical constituents (total nitrogen or sediment), but with the natural variability of biological constituents such as E. coli, the uncertainty of E. coli laboratory measurements, and the added complexity of modeling in a large drainage basin with multiple sources, these results are adequate and indicate that the explanatory variables being collected and models such as LOADEST can represent water-quality changes in the Missouri River for E. coli concentration from 2012 to 2016

Application of Differential Pulse Voltammetry to Determine the Efficiency of Stripping Tocopherols from Commercial Fish Oil

There has been an increase in the use of electrochemical methods for monitoring antioxidant levels in a variety of disciplines due to the sensitivity, low detection limits, ease of use, low cost and rapid analysis time offered by these techniques. One technique that has received specific attention is differential pulse voltammetry. We describe a novel application of differential pulse voltammetry to quantitatively and qualitatively determine the efficiency of removing tocopherols from commercial fish oil via column chromatographic separation. The relative limits of detection and quantitation of differential pulse voltammetry are compared to HPLC for determining the removal of tocopherols from commercial fish oil. It was determined that differential pulse voltammetry can monitor the separation of commercially added antioxidants from the bulk sample via a decrease in antioxidant oxidation currents. Furthermore, the limits of detection and quantitation were found to be comparable with values obtained using HPLC for tocopherol identification and quantitation.Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/141339/1/aocs0527.pd

Antagonisms, mutualisms and commensalisms affect outbreak dynamics of the southern pine beetle

Feedback from community interactions involving mutualisms are a rarely explored mechanism for generating complex population dynamics. We examined the effects of two linked mutualisms on the population dynamics of a beetle that exhibits outbreak dynamics. One mutualism involves an obligate association between the bark beetle, Dendroctonus frontalis and two mycangial fungi. The second mutualism involves Tarsonemus mites that are phoretic on D. frontalis ( commensal ), and a blue-staining fungus, Ophiostoma minus. The presence of O. minus reduces beetle larval survival ( antagonistic ) by outcompeting beetle-mutualistic fungi within trees yet supports mite populations by acting as a nutritional mutualist. These linked interactions potentially create an interaction system with the form of an endogenous negative feedback loop. We address four hypotheses: (1) Direct negative feedback: Beetles directly increase the abundance of O. minus, which reduces per capita reproduction of beetles. (2) Indirect negative feedback: Beetles indirectly increase mite abundance, which increases O. minus, which decreases beetle reproduction. (3) The effect of O. minus on beetles depends on mites, but mite abundance is independent of beetle abundance. (4) The effect of O. minus on beetles is independent of beetle and mite abundance. High Tarsonemus and O. minus abundances were strongly correlated with the decline and eventual local extinction of beetle populations. Manipulation experiments revealed strong negative effects of O. minus on beetles, but falsified the hypothesis that horizontal transmission of O. minus generates negative feedback. Surveys of beetle populations revealed that reproductive rates of Tarsonemus, O. minus, and beetles covaried in a manner consistent with strong indirect interactions between organisms. Co-occurrence of mutualisms embedded within a community may have stabilizing effects if both mutualisms limit each other. However, delays and/ or non-linearities in the interaction systems may result in large population fluctuations. © Springer-Verlag 2005

Determination of the Cyanide Metabolite 2-Aminothiazoline-4-Carboxylic Acid in Urine and Plasma by Gas Chromatography–mass Spectrometry

The cyanide metabolite 2-aminothiazoline-4-carboxylic acid (ATCA) is a promising biomarker for cyanide exposure because of its stability and the limitations of direct determination of cyanide and more abundant cyanide metabolites. A simple, sensitive, and specific method based on derivatization and subsequent gas chromatography–mass spectrometry (GC–MS) analysis was developed for the identification and quantification of ATCA in synthetic urine and swine plasma. The urine and plasma samples were spiked with an internal standard (ATCA-d2), diluted, and acidified. The resulting solution was subjected to solid phase extraction on a mixed-mode cation exchange column. After elution and evaporation of the solvent, a silylating agent was used to derivatize the ATCA. Quantification of the derivatized ATCA was accomplished on a gas chromatograph with a mass selective detector. The current method produced a coefficient of variation of less than 6% (intra- and interassay) for two sets of quality control (QC) standards and a detection limit of 25 ng/ml. The applicability of the method was evaluated by determination of elevated levels of ATCA in human urine of smokers in relation to non-smokers for both males and females

Frameless multimodal image guidance of localized convection-enhanced delivery of therapeutics in the brain

INTRODUCTION: Convection-enhanced delivery (CED) has been shown to be an effective method of administering macromolecular compounds into the brain that are unable to cross the blood-brain barrier. Because the administration is highly localized, accurate cannula placement by minimally invasive surgery is an important requisite. This paper reports on the use of an angiographic c-arm system which enables truly frameless multimodal image guidance during CED surgery. METHODS: A microcannula was placed into the striatum of five sheep under real-time fluoroscopic guidance using imaging data previously acquired by cone beam computed tomography (CBCT) and MRI, enabling three-dimensional navigation. After introduction of the cannula, high resolution CBCT was performed and registered with MRI to confirm the position of the cannula tip and to make adjustments as necessary. Adeno-associated viral vector-10, designed to deliver small-hairpin micro RNA (shRNAmir), was mixed with 2.0 mM gadolinium (Gd) and infused at a rate of 3 mul/min for a total of 100 mul. Upon completion, the animals were transferred to an MR scanner to assess the approximate distribution by measuring the volume of spread of Gd. RESULTS: The cannula was successfully introduced under multimodal image guidance. High resolution CBCT enabled validation of the cannula position and Gd-enhanced MRI after CED confirmed localized administration of the therapy. CONCLUSION: A microcannula for CED was introduced into the striatum of five sheep under multimodal image guidance. The non-alloy 300 mum diameter cannula tip was well visualized using CBCT, enabling confirmation of the position of the end of the tip in the area of interest