Correlation between ribonucleoside-diphosphate reductase and three replication proteins in Escherichia coli

<p>Abstract</p> <p>Background</p> <p>There has long been evidence supporting the idea that RNR and the dNTP-synthesizing complex must be closely linked to the replication complex or replisome. We contributed to this body of evidence in proposing the hypothesis of the replication hyperstructure. A recently published work called this postulate into question, reporting that NrdB is evenly distributed throughout the cytoplasm. Consequently we were interested in the localization of RNR protein and its relationship with other replication proteins.</p> <p>Results</p> <p>We tagged NrdB protein with 3×FLAG epitope and detected its subcellular location by immunofluorescence microscopy. We found that this protein is located in nucleoid-associated clusters, that the number of foci correlates with the number of replication forks at any cell age, and that after the replication process ends the number of cells containing NrdB foci decreases.</p> <p>We show that the number of NrdB foci is very similar to the number of SeqA, DnaB, and DnaX foci, both in the whole culture and in different cell cycle periods. In addition, interfoci distances between NrdB and three replication proteins are similar to the distances between two replication protein foci.</p> <p>Conclusions</p> <p>NrdB is present in nucleoid-associated clusters during the replication period. These clusters disappear after replication ends. The number of these clusters is closely related to the number of replication forks and the number of three replication protein clusters in any cell cycle period. Therefore we conclude that NrdB protein, and most likely RNR protein, is closely linked to the replication proteins or replisome at the replication fork. These results clearly support the replication hyperstructure model.</p

Modelos de representación de imprecisión e incertidumbre en fusión de alto nivel

Actas de: XVII Congreso Español sobre Tecnologías y Lógica Fuzzy (ESTYLF 2014). Zaragoza, 5-7 de febrero de 2014.Las técnicas de fusión de datos e información procedente de redes de sensores necesitan manejar información incierta e imprecisa, puesto que es habitual enfrentarse a problemas en los que el conocimiento disponible es vago o insuficiente y/o los aparatos de medición están sujetos a fallos. Con el reciente auge de la denominada "fusión de alto nivel", que tiene como objetivo reconocer la situación observada e identificar posibles riesgos, este problema se ha acentuado, ya que los formalismos que se utilizan habitualmente para construir un modelo simbólico del escenario, como la lógica de primer orden y las ontologías, no proporcionan soporte para este tipo de conocimiento. En este trabajo repasamos varias propuestas recientes para representación y razonamiento con información incierta e imprecisa en fusión de alto nivel. Nos centramos en dos tipos: (a) las que incorporan estos mecanismos en los propios modelos de representación, como las ontologías probabilísticas y difusas y las redes lógicas de Markov; (b) las que extienden el proceso de fusión con una capa de gestión de incertidumbre adicional, como las basadas en argumentación probabilística.Este trabajo ha sido financiado por la Junta de Andalucía (P11-TIC-7460), la Comunidad de Madrid (S2009/TIC- 1485) y el Ministerio de Economía y Competitividad de España (TEC2012-37832-C02-01, TEC2011-28626-C02- 02, TIN2012-30939).Publicad

Madres malas y literatura del exilio

Existe un denominador común a una serie escritores contemporáneos de origen español pero de expresión francesa, como Agustín Gómez Arcos, Jorge Semprún o Michel del Castillo, que se materializa como una temática recurrente en sus obras. En casi todas sus novelas puede descubrirse una mujer «mala» y, para más señas, la figura de la madre y la necesidad que tienen de hablar de ella. ¿Qué ocurre cuando la madre, real o simbólica, resulta ser mala, bien porque reniegue de su función principal, la maternidad, o de su función social? Madrastra también la madre patria que ha expulsado de su regazo a estos autores, entonces niños de la guerra. En sus imaginarios, estas dos realidades están demasiado cercanas para evitar que no se confundan. España se ha convertido en una madrastra para ellos, una madre cruel que les fuerza al exilio y les obliga a renegar de su lengua.There is a common factor to several contemporary authors of Spanish origin but with a French expression, such as Agustín Gómez Arcos, Jorge Semprún or Michel del Castillo, which comes to a recurrent subject matter in their works. In nearly all of their works we can find out an «evil» woman and, to be more specific, the mother figure and the necessity they have to talk about it. What is the matter when that mother, true or symbolic, turns out to be evil by denying either her main duty —motherhood— or her social one? A stepmother also is the mother country that has expelled these writers —then war babies— from their lap. In their fiction these two realities are too close as not to be confused. Spain has become a stepmother to them, a cruel mother who sends them into exile and makes them deny their mother tongue

Traducción y memoria histórica: El niño pan de Agustín Gómez Arcos

Traduction et mémoire historique s’épousent de manière spéciale dans le cas de la réception des auteurs espagnols d’expression française au XXe siècle. À l’orée du XXIe siècle et grâce à la traduction, ces auteurs deviennent accessibles dans leur langue maternelle et ils nous offrent le témoignage d’une littérature qui porte en elle, soixante-dix ans après, les stigmates d’un passé confronté à la guerre civile, à l’exil et un engagement contre le régime politique qui s’ensuit. Le cas d’Agustín Gómez Arcos s’intègre parfaitement dans cette perspective, car son thème fétiche c’est l’Espagne franquiste et sa mémoire historique est tissée de mémoire linguistique.Translation and historical memory unite in a very special way in the case of the reception of Spanish authors writing in French. In the 21st century these authors are getting accessible in their mother tongue. Sixty years later they evidence a literature with the marks of a past confronting Spanish Civil War, exile and the commitment to oppose the new political regime. Agustín Gómez Arcos fits perfectly into this perspective for his fetish topic is Francoist Spain and his historical memory has linguistic memory too

Persistence of Zika Virus in Body Fluids — Final Report

Zika Virus; Body FluidsVirus Zika; Fluids corporalsFluidos corporalesPaz-Bailey and colleagues (Sept. 27 issue)1 describe the dynamics of Zika virus (ZIKV) in body fluids in a cohort of participants who lived in an area where the virus was endemic and in whom ZIKV infection was detected on reverse-transcriptase–polymerase-chain-reaction assay. We are concerned about the external validity of these results

Ontology-based context representation and reasoning for object tracking and scene interpretation in video

Computer vision research has been traditionally focused on the development of quantitative techniques to calculate the properties and relations of the entities appearing in a video sequence. Most object tracking methods are based on statistical methods, which often result inadequate to process complex scenarios. Recently, new techniques based on the exploitation of contextual information have been proposed to overcome the problems that these classical approaches do not solve. The present paper is a contribution in this direction: we propose a Computer Vision framework aimed at the construction of a symbolic model of the scene by integrating tracking data and contextual information. The scene model, represented with formal ontologies, supports the execution of reasoning procedures in order to: (i) obtain a high-level interpretation of the scenario; (ii) provide feedback to the low-level tracking procedure to improve its accuracy and performance. The paper describes the layered architecture of the framework and the structure of the knowledge model, which have been designed in compliance with the JDL model for Information Fusion. We also explain how deductive and abductive reasoning is performed within the model to accomplish scene interpretation and tracking improvement. To show the advantages of our approach, we develop an example of the use of the framework in a video-surveillance application.This work was supported in part by Projects CICYT TIN2008- 06742-C02-02/TSI, CICYT TEC2008-06732-C02-02/TEC, SINPROB, CAM MADRINET S-0505/TIC/0255 and DPS2008–07029-C02–02.Publicad

Information fusion from multiple databases using meta-association rules

Nowadays, data volume, distribution, and volatility make it difficult to search global patterns by applying traditional Data Mining techniques. In the case of data in a distributed environment, sometimes a local analysis of each dataset separately is adequate but some other times a global decision is needed by the analysis of the entire data. Association rules discovering methods typically require a single uniform dataset and managing with the entire set of distributed data is not possible due to its size. To address the scenarios in which satisfying this requirement is not practical or even feasible, we propose a new method for fusing information, in the form of rules, extracted from multiple datasets. The proposed model produces meta-association rules, i.e. rules in which the antecedent or the consequent may contain rules as well, for finding joint correlations among trends found individually in each dataset. In this paper, we describe the formulation and the implementation of two alternative frameworks that obtain, respectively, crisp meta-rules and fuzzy meta-rules. We compare our proposal with the information obtained when the datasets are not separated, in order to see the main differences between traditional association rules and meta-association rules. We also compare crisp and fuzzy methods for meta-association rule mining, observing that the fuzzy approach offers several advantages: it is more accurate since it incorporates the strength or validity of the previous information, produces a more manageable set of rules for human inspection, and allows the incorporation of contextual information to the mining process expressed in a more human-friendly format

Ontological representation of context knowledge for visual data fusion

8 pages, 4 figures.-- Contributed to: 12th International Conference on Information Fusion, 2009 (FUSION '09, Seattle, Washington, US, Jul 6-9, 2009).Context knowledge is essential to achieve successful information fusion, especially at high JDL levels. Context can be used to interpret the perceived situation, which is required for accurate assessment. Both types of knowledge, contextual and perceptual, can be represented with formal languages such as ontologies, which support the creation of readable representations and reasoning with them. In this paper, we present an ontology-based model compliant with JDL to represent knowledge in cognitive visual data fusion systems. We depict the use of the model with an example on surveillance. We show that such a model promotes system extensibility and facilitates the incorporation of humans in the fusion loop.This work was supported in part by Projects CICYT TIN2008-06742-C02-02/TSI, CICYT TEC2008-06732-C02-02/TEC, SINPROB, CAM MADRINET S-0505/TIC/0255 and DPS2008-07029-C02-02.Publicad

Communication in distributed tracking systems: an ontology-based approach to improve cooperation

Current Computer Vision systems are expected to allow for the management of data acquired by physically distributed cameras. This is especially the case for modern surveillance systems, which require communication between components and a combination of their outputs in order to obtain a complete view of the scene. Information fusion techniques have been successfully applied in this area, but several problems remain unsolved. One of them is the increasing need for coordination and cooperation between independent and heterogeneous cameras. A solution to achieve an understanding between them is to use a common and well-defined message content vocabulary. In this research work, we present a formal ontology aimed at the symbolic representation of visual data, mainly detected tracks corresponding to real-world moving objects. Such an ontological representation provides support for spontaneous communication and component interoperability, increases system scalability and facilitates the development of high-level fusion procedures. The ontology is used by the agents of Cooperative Surveillance Multi-Agent System, our multi-agent framework for multi-camera surveillance systems.This work was supported in part by Projects CICYT TIN2008-06742-C02-02=TSI, CICYT TEC2008-06732-C02-02=TEC, CAM CONTEXTS (S2009=TIC-1485) and DPS2008-07029-C02-02.Publicad