Comparison Extraction Methods of Essential oils of Rosmarinus officinalis L. in Iran By Microwave Assisted Water Distillation; Water Distillation and Steam Distillation

Rosmarinus officinalis L. is a perennial herb that belongs to the Lamiaceae family. It is used as a food flavouring agent, and well known medicinally for its powerful antimutagenic, antibacterial and chemopreventive properties. The most applicable method for extraction of essential oil is water distillation (WD) and steam distillation (SD). It is a traditional technique which is used in most industrious companies. The essential oil from the leaves rosemary obtained by Microwave assisted water distillation (MAWD) on 440W, 770W and 1100W has been compared with those obtained by conventional WD and SD. The total yield of the volatile fractions obtained through WD was 1.30%, SD was 0.54% and MAWD in 440W was 0.45%, 770 W was 0.50% and 1100 W was 0.55%, respectively.Essential oils samples were analyzed by GC and GC-MS, the oils revealed the presence of 28 to 35 compounds in the essential oils obtained through HD, SD and MAWD, respectively. Main components in WD method were camphene 33.08%, γ-terpinene 8.62% and verbenene 8.57%, in SD were camphene 31.71%, WD, γ-terpinene 8.92%, and verbenene 8.74%, and by Microwave-assisted water-distillation (MAWD) on 440W were linalool acetate 28.67% cis-sabinene hydrate acetate 20.59% and terpin-4-ol 11.56%,  on 770W were camphene 15.88%, γ-terpinene 14.61% and cis-sabinene hydrate acetate 10.28%, and on 1100W were camphene 28.22%, γ-terpinene 13.66% and β-pinene 8.42%, respectively

Chemical Composition of the Essential Oil of Two Tanacetum Species from Iran

The genus Tanacetum L. is one of the most important medicinal plant that contains 28 species in Iran, 12 of them are endemics. This paper reports the essential oil composition of Tanacetum hololeucum (Bornm.) Podlech; Tanacetum kotschyi (Boiss.) Grierson, growing wild in Iran. Plant flowers were collected from different zones of Dizin in north of Iran. All samples were hydro-distilled (Clevenger apparatus), to produce the oils in T. hololeucum from three zones with yields of (0.35%, 0.35% and 0.16%), and also T. kotschyi, from three zones with yields of (0.57%, 0.41% and 0.10%), respectively. All samples were injected to GC and GC/MS. Main components for T.hololeucum from Dizin of three places from zone 1 were borneol 39.4%, trans- sabinene hydrate 8.4%, 1,8-cineole 8.1%, and from zone 2 were n-hexadecane 14%,  elemol acetate 13.6%, trans-thujone 13.4%,  and from zone 3  were terpinolene 35.7%,  α- phellandrene 23%, γ- eudesmol 7.1% , and in T. kotschyi   also from Dizin of three places from zone 1 were 1,8-cineole 27.8%, terpinolene 13%, trans-thujone 7.2%, and from zone  2  were n-undecane 27.9%, n-hexadecane 14.7%, n-nonadecane 8.7% , and from zone 3 were n-tridecane 55.1%, terpinolene 13.4%, myrcene 7%, respectively

Chemical Composition of the Essential Oil of Tanacetum uniflorum (Fisch. & C.A. Mey. ex DC.) Sch.Bip. from (Salmas) North-West of Iran

The genus Tanacetum L. belongs to the Asteraceae family and Anthemideae tribe is one of the most important medicinal plants that contains 26 species in Iran, 12 of them are endemic. This paper reports the essential oil composition of Tanacetum uniflorum (Fisch. & C.A. Mey. ex DC.) Sch.Bip. growing spontaneously in Salmas (North-West of Iran). Essentail oil extracted by water distillation and steam distillation from Plant on flowers and leaves were collected on July - 2016. The yields of water distillation from leaf were 0.12% and flower were 1.16% (V/W), and by steam distillation from leaf were 0.33% and flower were 0.76% (V/W), respectively, then samples were analyzed by GC and GC/MS. Main components obtained from water distillation from leaf were manoyl oxide 28.87% , α-cadinol 9.7% and γ-eudesmol acetate 4.77%  and in  flower were methyl pentanoate 88.28%, butyl acetate 5.13% and iso-dihydro carveol acetate 1.19% (V/W), and by steam distillation from leaf were γ- terpinene  17.99%, endo-fenchyl acetate 9.63% and butyl acetate 8.12% and in flower were methyl pentanoate 79.9%,  bulnesol 2.70% and (E )-nerolidolol acetate 2.69% obtained, respectively

Effect of Harvesting Time on Content and Chemical Composition of Essential Oil from Stachys lavandulifolia Vahl (Lamiaceae)

Stachys L. one of the biggest genus of the Lamiaceae family comprises about 200-300 species.  In this research, aerial parts essential oil composition and content of Stachys lavandulifolia Vahl (Lamiaceae)at different stages (vegetative, full flowering and initial fruiting stages) is reported. The oils obtained by water distillation method (Clevenger apparatus) and analyzed by GC and GC/MS. The results showed that the essential oils of S. lavandulifolia were affected by plant growth stages. The chemical composition varied in three harvesting times. It was found that the maximum obtained essential oil was in the flowering stage. Totally, 31 constituents with the range of 0.10 – 34.11% in the vegetative, 27 constituents with the range of 0.06 – 36.35% in the flowering stage and 27 compounds with the range of 0.06 – 37.2% in the initial fruiting stages were identified. The highest compounds were related to the vegetative stage (34 compounds) that representing 63.74% of oil.  In this study, the highest amount of essential oil constituents in the vegetative stage belonged to germacren D (34.11%), n-decane (3.84%) and caryophyllene oxide (2.62%), in the flowering stage, germacren D (36.87%), borneol (4.3%), cis-thujone (4.24%), bicyclogermacrene (4.16%) & n-decane (3.88%) and in the stage of initial fruiting, germacren D (37.2%), borneol (4.76%), b-caryophyllene (4.20%), cis-thujone (4.16%) & bicyclogermacrene (3.99%)

Increasing the Number of Adverse Drug Reactions Reporting: the Role of Clinical Pharmacy Residents

Abstract Detection of adverse drug reactions (ADRs) in hospitals provides an important measure of the burden of drug related morbidity on the healthcare system. Spontaneous reporting of ADRs is scare and several obstacles to such reporting have been identified formerly. This study aimed to determine the role of clinical pharmacy residents in ADR reporting within a hospital setting.Clinical pharmacy residents were trained to report all suspected ADRs through ADRreporting yellow cards. The incidence, pattern, seriousness, and preventability of the reported ADRs were analyzed. During the period of 12 months, for 8559 patients, 202 ADR reports were received. The most frequently reported reactions were due to anti-infective agents (38.38%). Rifampin accounted for the highest number of the reported ADRs among anti-infective agents. The gastro-intestinal system was the most frequently affected system (21.56%) of all reactions. Fifty four of the ADRs were reported as serious reactions. Eighteen of the ADRs were classified as preventable. Clinical pharmacy residents involvement in the ADR reporting program could improve the ADR reporting system

Global age-sex-specific mortality, life expectancy, and population estimates in 204 countries and territories and 811 subnational locations, 1950–2021, and the impact of the COVID-19 pandemic: a comprehensive demographic analysis for the Global Burden of Disease Study 2021

Background: Estimates of demographic metrics are crucial to assess levels and trends of population health outcomes. The profound impact of the COVID-19 pandemic on populations worldwide has underscored the need for timely estimates to understand this unprecedented event within the context of long-term population health trends. The Global Burden of Diseases, Injuries, and Risk Factors Study (GBD) 2021 provides new demographic estimates for 204 countries and territories and 811 additional subnational locations from 1950 to 2021, with a particular emphasis on changes in mortality and life expectancy that occurred during the 2020–21 COVID-19 pandemic period. Methods: 22 223 data sources from vital registration, sample registration, surveys, censuses, and other sources were used to estimate mortality, with a subset of these sources used exclusively to estimate excess mortality due to the COVID-19 pandemic. 2026 data sources were used for population estimation. Additional sources were used to estimate migration; the effects of the HIV epidemic; and demographic discontinuities due to conflicts, famines, natural disasters, and pandemics, which are used as inputs for estimating mortality and population. Spatiotemporal Gaussian process regression (ST-GPR) was used to generate under-5 mortality rates, which synthesised 30 763 location-years of vital registration and sample registration data, 1365 surveys and censuses, and 80 other sources. ST-GPR was also used to estimate adult mortality (between ages 15 and 59 years) based on information from 31 642 location-years of vital registration and sample registration data, 355 surveys and censuses, and 24 other sources. Estimates of child and adult mortality rates were then used to generate life tables with a relational model life table system. For countries with large HIV epidemics, life tables were adjusted using independent estimates of HIV-specific mortality generated via an epidemiological analysis of HIV prevalence surveys, antenatal clinic serosurveillance, and other data sources. Excess mortality due to the COVID-19 pandemic in 2020 and 2021 was determined by subtracting observed all-cause mortality (adjusted for late registration and mortality anomalies) from the mortality expected in the absence of the pandemic. Expected mortality was calculated based on historical trends using an ensemble of models. In location-years where all-cause mortality data were unavailable, we estimated excess mortality rates using a regression model with covariates pertaining to the pandemic. Population size was computed using a Bayesian hierarchical cohort component model. Life expectancy was calculated using age-specific mortality rates and standard demographic methods. Uncertainty intervals (UIs) were calculated for every metric using the 25th and 975th ordered values from a 1000-draw posterior distribution. Findings: Global all-cause mortality followed two distinct patterns over the study period: age-standardised mortality rates declined between 1950 and 2019 (a 62·8% [95% UI 60·5–65·1] decline), and increased during the COVID-19 pandemic period (2020–21; 5·1% [0·9–9·6] increase). In contrast with the overall reverse in mortality trends during the pandemic period, child mortality continued to decline, with 4·66 million (3·98–5·50) global deaths in children younger than 5 years in 2021 compared with 5·21 million (4·50–6·01) in 2019. An estimated 131 million (126–137) people died globally from all causes in 2020 and 2021 combined, of which 15·9 million (14·7–17·2) were due to the COVID-19 pandemic (measured by excess mortality, which includes deaths directly due to SARS-CoV-2 infection and those indirectly due to other social, economic, or behavioural changes associated with the pandemic). Excess mortality rates exceeded 150 deaths per 100 000 population during at least one year of the pandemic in 80 countries and territories, whereas 20 nations had a negative excess mortality rate in 2020 or 2021, indicating that all-cause mortality in these countries was lower during the pandemic than expected based on historical trends. Between 1950 and 2021, global life expectancy at birth increased by 22·7 years (20·8–24·8), from 49·0 years (46·7–51·3) to 71·7 years (70·9–72·5). Global life expectancy at birth declined by 1·6 years (1·0–2·2) between 2019 and 2021, reversing historical trends. An increase in life expectancy was only observed in 32 (15·7%) of 204 countries and territories between 2019 and 2021. The global population reached 7·89 billion (7·67–8·13) people in 2021, by which time 56 of 204 countries and territories had peaked and subsequently populations have declined. The largest proportion of population growth between 2020 and 2021 was in sub-Saharan Africa (39·5% [28·4–52·7]) and south Asia (26·3% [9·0–44·7]). From 2000 to 2021, the ratio of the population aged 65 years and older to the population aged younger than 15 years increased in 188 (92·2%) of 204 nations. Interpretation: Global adult mortality rates markedly increased during the COVID-19 pandemic in 2020 and 2021, reversing past decreasing trends, while child mortality rates continued to decline, albeit more slowly than in earlier years. Although COVID-19 had a substantial impact on many demographic indicators during the first 2 years of the pandemic, overall global health progress over the 72 years evaluated has been profound, with considerable improvements in mortality and life expectancy. Additionally, we observed a deceleration of global population growth since 2017, despite steady or increasing growth in lower-income countries, combined with a continued global shift of population age structures towards older ages. These demographic changes will likely present future challenges to health systems, economies, and societies. The comprehensive demographic estimates reported here will enable researchers, policy makers, health practitioners, and other key stakeholders to better understand and address the profound changes that have occurred in the global health landscape following the first 2 years of the COVID-19 pandemic, and longer-term trends beyond the pandemic

Global burden of peripheral artery disease and its risk factors, 1990–2019 : a systematic analysis for the Global Burden of Disease Study 2019

peripheral artery disease were modelled using the Global Burden of Disease, Injuries, and Risk Factors Study (GBD) 2019 database. Prevalence, disability-adjusted life years (DALYs), and mortality estimates of peripheral artery disease were extracted from GBD 2019. Total DALYs and age-standardised DALY rate of peripheral artery disease attributed to modifiable risk factors were also assessed. Findings In 2019, the number of people aged 40 years and older with peripheral artery disease was 113 million (95% uncertainty interval [UI] 99·2–128·4), with a global prevalence of 1·52% (95% UI 1·33–1·72), of which 42·6% was in countries with low to middle Socio-demographic Index (SDI). The global prevalence of peripheral artery disease was higher in older people, (14·91% [12·41–17·87] in those aged 80–84 years), and was generally higher in females than in males. Globally, the total number of DALYs attributable to modifiable risk factors in 2019 accounted for 69·4% (64·2–74·3) of total peripheral artery disease DALYs. The prevalence of peripheral artery disease was highest in countries with high SDI and lowest in countries with low SDI, whereas DALY and mortality rates showed U-shaped curves, with the highest burden in the high and low SDI quintiles. Interpretation The total number of people with peripheral artery disease has increased globally from 1990 to 2019. Despite the lower prevalence of peripheral artery disease in males and low-income countries, these groups showed similar DALY rates to females and higher-income countries, highlighting disproportionate burden in these groups. Modifiable risk factors were responsible for around 70% of the global peripheral artery disease burden. Public measures could mitigate the burden of peripheral artery disease by modifying risk factors

The Study of Nosocomial Infections in Neonatal Intensive Care Unit, A prospective study in Northwest Iran

Background: Nosocomial infections are an important cause of mortality in neonatal intensive care units (NICUs). Therefore, in this study, the incidence and prevalence of nosocomial infections were determined in NICUs of the three largest neonatal centers in northwest Iran, and the causative bacteria were identified in order to provide potential solutions to control the infections in these hospitals. Materials and Methods: This is a descriptive-prospective study in which the cases of nosocomial infections were examined in the three largest hospitals in Tabriz in northwest Iran during 1 year (from June 2012 until May 2013) based on clinical findings, medical and nursing reports of patients, and laboratory results. Results: Of the 3129 patients hospitalized in NICUs of the three hospitals, 208 patients were diagnosed with nosocomial infections. The incidence rate of nosocomial infections was 11.34%.per 100 patient days with 52.4% bacteremia, 32.69% pneumonia, 5.77% urinary tract infections, 5.29% wound infections, and 3.85% necrotizing enterocolitis. There was a statistically significant relationship between invasive procedures (such as umbilical catheters, central venous catheters, surgery, and TPN) and sepsis (P = 0.001). The relationships between urinary tract infection and urinary catheter (P = 0.000), and aggressive procedures (such as suctioning and intubation) and pneumonia (P = 0.001) were also statistically significant. Conclusion: Incidence of nosocomial infections in premature and low birth weight newborns is considered as a health threat. The findings of this research reiterate the importance of giving further attention to prevention and control of nosocomial infections in the NICU

Cytobiochemical Potentials of Rosa damascena Mill. Extract

ABSTRACT Background and Objectives: There is a profound inclination among people toward consumptio