Pseudomonas rhizophila S211, a New Plant Growth-Promoting Rhizobacterium with Potential in Pesticide-Bioremediation

A number of Pseudomonas strains function as inoculants for biocontrol, biofertilization, and phytostimulation, avoiding the use of pesticides and chemical fertilizers. Here, we present a new metabolically versatile plant growth-promoting rhizobacterium, Pseudomonas rhizophila S211, isolated from a pesticide contaminated artichoke field that shows biofertilization, biocontrol and bioremediation potentialities. The S211 genome was sequenced, annotated and key genomic elements related to plant growth promotion and biosurfactant (BS) synthesis were elucidated. S211 genome comprises 5,948,515 bp with 60.4% G+C content, 5306 coding genes and 215 RNA genes. The genome sequence analysis confirmed the presence of genes involved in plant-growth promoting and remediation activities such as the synthesis of ACC deaminase, putative dioxygenases, auxin, pyroverdin, exopolysaccharide levan and rhamnolipid BS. BS production by P. rhizophila S211 grown on olive mill wastewater based media was effectively optimized using a central-composite experimental design and response surface methodology (RSM). The optimum conditions for maximum BS production yield (720.80 ± 55.90 mg/L) were: 0.5% (v/v) inoculum size, 15% (v/v) olive oil mill wastewater (OMWW) and 40◦C incubation temperature at pH 6.0 for 8 days incubation period. Biochemical and structural characterization of S211 BS by chromatography and spectroscopy studies suggested the glycolipid nature of the BS. P. rhizophila rhamnolipid was stable over a wide range of temperature (40–90◦C), pH (6–10), and salt concentration (up to 300mM NaCl). Due to its low-cost production, emulsification activities and high performance in solubilization enhancement of chemical pesticides, the indigenous BS-producing PGPR S211 could be used as a promising agent for environmental bioremediation of pesticide-contaminated agricultural soils

[(1R,4S)-(+)-3-Benzoyl-1,7,7-trimethyl­bicyclo­[2.2.1]heptan-2-olato-κ2 O 2,O 3](η4-norbornadiene)rhodium(I)

In the title complex mol­ecule, [Rh(C17H19O2)(C7H8)], the rhodium(I) metal centre is coordinated by the O atoms of a benzoyl­camphorate anion and the C=C bonds of the norbornadiene mol­ecule into a slightly distorted square-planar coordination geometry. The six-membered chelate ring is essentially planar (r.m.s. deviation = 0.0378 Å) and forms a dihedral angle of 31.67 (11)° with the phenyl ring

Informe de la campaña ARGELIA0204 para la evaluación de recursos demersales en las costas de Argelia (Mediterráneo sud-occidental)

RESUMEN: Del 5 de Febrero al 8 de Marzo de 2004 se llevó a cabo la campaña de prospección pesquera con arrastre de fondo ARGELIA0204 en aguas de Argelia, a bordo del B/O Vizconde de Eza. Se realizaron 114 pescas (6 nulas) entre 40 y 760 m de profundidad. Se presentan los resultados de las capturas realizadas en esta campaña y se analizan los índices de abundancia y biomasa de las especies más importantes, así como su distribución de tallas, por sector geográfico y estrato batimétrico. Se comparan los rendimientos y las tallas obtenidos con los de la campaña realizada en 2003 en la misma área y con otras campañas realizadas en el Mediterráneo.RÉSUME: Rapport de la campagne ARGELIA0204 portant évaluation des ressources demersaux des côtes Algériennes (Méditerranée sud-occidental).- Du 05 du Février au 8 du Mars 2004, s’est réalisée une campagne de prospection par chalutage du fond ARGELIA0204 des côtes Algériennes, a bord du B/O Vizconde de Eza. 114 traits de pêche (6 annulées) ont été effectue entre 40 et 800 m de profondeur. Les résultats des captures réalisées durant cette campagne et l’analyse des indices d’abondance et biomasse des espèces les plus importantes sont présentés, par secteur, par aire géographique et strate bathymétrique, ainsi que leurs distributions de tailles par secteur géographique et strate bathymétrique. Les rendements et les tailles obtenus ont fait l’objet d’une comparaison avec les autres campagnes réalisées dans la Méditerranée.ABSTRACT: Report of the ARGELIA0204 survey for the assessment of the deep-sea demersal resources off Algerian coast (south-western Mediterranean).- From 5 February to 8 March 2004 the bottom-trawl survey ARGELIA0204 was developed in waters off Argelia, on board R/V Vizconde de Eza. 114 hauls (6 null) were carried out between 40 and 800 m depth. The results on the captures obtained during this survey are shown and abundance and biomass indexes of the most important species are analyzed by geographic sector and area, and bathymetric stratum, as their size distribution by geographic sector and bathymetric stratum. Yields and sizes obtained are compared to those from other surveys carried out in the Mediterranea

Elaboration et etude des proprietes magnetiques et structurales des couches minces amorphes de Co_1_-_x_-_yZr_xM_y avec M=Zr, Nb, Ti, Pt

SIGLEINIST T 77198 / INIST-CNRS - Institut de l'Information Scientifique et TechniqueFRFranc

UNCERTAINTY IN THE PERT’S CRITICAL PATH

In this paper, the problem of scheduling is addressed. Due to difficulties in scheduling projects, researchers and professionals have proposed a tremendous number of works aiming at finding the best method to accomplish this phase of any project, especially if the decision maker is facing the challenge of uncertain estimations. One of the most used families of techniques is discussed in this paper, namely the Fuzzy Program Evaluation and Review Technique techniques. This family of techniques is based mainly on using the classical Program Evaluation and Review Technique and the fuzzy set theory. This work presents a comparison between two interesting techniques used to tackle the problem of uncertainty, namely the Model for Project Scheduling with Fuzzy Precedence Links and the Centroid techniques. The first technique is based on the relationship strength between each two activities in order to resolve the problem of the critical path. The second technique is based on a very simple mathematical concept and arithmetic of fuzzy numbers to tackle the same problem. Based on the results of a numerical example, we noticed that the simplicity and inexpensiveness of the Centroid method beat the complicated and expensive characteristics of the Model for Project Scheduling with Fuzzy Precedence Links

Phase equilibria in the NaF-CdO-NaPO3 system at 873K and crystal structure and physico-chemical characterizations of the new Na2CdPO4F fluorophosphates.

International audienceIsothermal sections of the diagram representing phase relationships in the NaF-CdO-NaPO3 system have been investigated by solid state reactions and powder X-ray diffraction. This phase diagram investigation confirms the polymorphism of the NaCdPO4 side component and the structure of the ß high temperature polymorph (orthorhombic, space group Pnma and unit cell parameters a=9.3118(2), b=7.0459(1), c=5.1849(1) Å has been refined. A new fluorophosphate, Na2CdPO4F, has been discovered and its crystal structure determined and refined from powder X-ray diffraction data. It exhibits a new 3D structure with orthorhombic symmetry, space group Pnma and unit cell parameters a=5.3731(1), b=6.8530(1), c=12.2691(2) Å. The structure is closely related to those of the high temperature polymorph of the nacaphite Na2CaPO4F and the fluorosilicate Ca2NaSiO4F but differs essentially in the cationic repartition since the structure is fully ordered with one Na site (8d) and one Cd site (4c). Relationships with other Na2MIIPO4F (MII=Mg, Ca, Mn, Fe, Co, Ni) have been examined and the crystal-chemical and topographical analysis of these fluorophosphates is briefly reviewed. IR, Raman, optical and 19F, 23Na, 31P MAS NMR characterizations of Na2CdPO4F have been investigated

Characteristics of leachate upon hydrothermal treatment processing: case study of Ampang district municipal solid waste leachate

Municipal solid waste (MSW) leachate is a hazardous liquid produced from decomposition of solid waste with high amount of organic matter and ammonia-nitrogen with obnoxious smell. This study aimed to investigate the behavior of MSW leachate when subjected to hydrothermal treatment using an autoclave set up at below water critical points (temperatures of 100 °C, 150 °C, and 200 °C at 0.1 MPa, 0.4 MPa and 1.6 MPa, respectively) with 15 min and 60 min holding time. Physicochemical characterization of the setup at 200 °C and 1.6 MPa at 60 min holding time indicates a feasible parameter when materials that caused the dark color and obnoxious smell were almost completely removed. Over 99% of chemical oxygen demand and ammonia nitrogen was eliminated when treated with hydrothermal treatment and yielded a condensed liquid product that complied with permissible limits set by the National Water Quality Standard Malaysia and the World Health Organization for wastewater discharges for irrigation purposes. Chromatographic analysis indicated that most of the organic compounds present in the raw leachate was removed. This processing is believed to be an environmentally friendly method that can treat MSW leachate rapidly, and it has the potential to be used as an effective alternative to existing leachate treatment technologies

Seismic Vulnerability Assessment at Urban Scale: Case of Algerian buildings

International audienc

A novel frameshift mutation (c.405delC) in the GJB2 gene associated with autosomal recessive hearing loss in two Tunisian families.

International audienceOBJECTIVES: Mutations in GJB2 are found to be responsible for 50% of congenital autosomal recessive non-syndromic hearing loss, one of the most important mutations in this gene is the c.35delG, which is responsible for the majority of GJB2 related deafness in the Tunisian population. The aim of this study was to determine the molecular etiology of hearing loss in two Tunisian individuals. METHODS: We screened two Tunisian individuals affected by congenital, bilateral, profound, sensorineural hearing loss for mutations in GJB2 gene using PCR and direct sequencing. RESULTS: We identified a novel frameshift mutation in the GJB2 gene, the c.405delC resulting in a truncated protein (p.Tyr136Thrfs*32). It was found in compound heterozygosity with the c.35delG in two non-consanguineous unrelated families from Tunisia. One patient underwent a cochlear implant at 4 years. Initial evaluations post-implantation indicate a successful cochlear implant outcome since the patient began to acquire language abilities and auditory sensation. CONCLUSIONS: With this novel GJB2 mutation, the mutational spectrum of this gene continues to broaden in our population. The occurrence of biallelic GJB2 mutations for the other deaf girl, despite the neonatal pain and hypotension due to complicated delivery, led us to confirm the importance of GJB2 screening for cochlear implant candidates regardless of the etiology of deafness in populations with a relatively high frequency of GJB2 mutation carriers