Almost Mirror Image: Exploring The Similarities And Dissimilarities Of Identical Twins In Theatrical Solo Performance

Almost Mirror Image: Exploring the Similarities and Dissimilarities of Identical Twins in Theatrical Solo Performance Is an exploration of the psyche of identical twins within the context of devised solo performance. The author, an identical twin herself, has long been interested in twins\u27 ability to cultivate both highly independent personalities as well as intensely co-dependent tendencies during development. What can twins tell us about the way we create close relationships and how is their upbringing radically different from the majority of the world that is born alone? Equally intrigued by society\u27s growing technological dependence, the author would like to delve into how the science and development of twins appears counterintuitive to the intra-personal technological world they grow up in by using personal, autobiographic solo performance as her research platform. The data collected from research sources such as Jo Bonney\u27s Extreme Exposure and Michael Kearns\u27 The Solo Performer\u27s Journey, will provide fodder for the thesis document and the author\u27s devised solo piece, entitled Teach me how to be Lonely. While devising her own solo performance, the author will compare and contrast her process with that of a few select solo performers such as Anna Deavere Smith and Rachel Rosenthal. The author will delve into various styles of solo work creation, including the testimony plays of Smith and the autobiographical style of Rosenthal, in order to view her own work with a self-reflective and identity-driven lens. Overall, the author hopes to achieve a more comprehensive understanding of her own experience as an identical twin through the facilitation of her solo work as well as explore how the creation of solo performance can offer artists in the 21st century more freedom of expression and identity than the performance of a standard play

Modularisation as enabler of circular economy in energy infrastructure

Existing energy infrastructure have a technical and/or economic lifecycle predetermined by the lifetime of certain components. In energy infrastructure, the residual lifetime of civil structure or other components with a longer life is usually wasted. Modular energy infrastructure can be reconfigurable decoupling the life of the infrastructure from their modules, and extending module and/or infrastructure lifecycle. Modularisation could become a cornerstone to enable circular economy (CE) and enhanced sustainability. Remarkably, despite the growing interest among policymakers, practitioners and academics in both CE and modularisation, there is a lack of knowledge about the link between CE and modularisation in energy infrastructure. Through a Systematic Literature Review, this paper derives the gap in knowledge regarding the link between CE and modularisation in energy infrastructure. This link is then investigated in other sectors identifying relevant implications such as reduction of construction waste and achievement of the closed-loop material cycle. Furthermore, the case of Yamal Liquefied Natural Gas project is used to compare and contrast two perspectives: “Traditional modularisation” and “Modular CE”. Lastly, the paper discusses existing policies, provides policy recommendations to foster “Modular CE” in energy infrastructure and suggests a research agenda

Éponges à microARN, artificielles ou naturelles, dans le contexte de la transformation tumorale

La sénescence se caractérise par un arrêt en phase G1/S du cycle cellulaire et peut être induit par une variété de stress tels que des télomères trop courts, l’activation d’oncogène ou encore à cause de stress oxydatifs. Cette réponse cellulaire s’accompagne de profonds changements au niveau de l’expression génique et les ARN non codants sont d’importants acteurs de ceux-ci. Bien que cette catégorie d’ARN ait longtemps été considérée comme un sous-produit non fonctionnel de la transcription, on sait maintenant qu’ils sont impliqués dans une pléthore de fonctions essentielles à l’homéostasie de la cellule. Les microARN (miR), de petits ARN non codants d’une vingtaine de nucléotides, sont souvent diminués ou surexprimés dans les maladies, soulignant leurs rôles importants dans le développement de celles-ci. C’est le cas notamment de deux oncomirs, miR-19 et miR-155, qui s’accumulent de manière aberrante dans les cancers hématopoïétiques. En condition normale, STAT5A, qui est souvent dérégulé dans ces cancers, induit SOCS1 qui agit comme un frein sur cette voie de signalisation afin de prévenir une prolifération incontrôlée. Des travaux conduits dans notre laboratoire montrent que SOCS1 est aussi impliqué dans la sénescence, car il est capable d’activer p53, un important suppresseur tumoral. SOCS1 peut être ciblé par les deux oncomirs et nos résultats montrent qu’une inhibition de ces derniers à l’aide d’éponges artificielles favorisait l’accumulation d’un p53 actif. De plus, en intégrant le ribozyme à tête de marteau dans la conception des éponges, nous avons créé une nouvelle génération d’outils (éponges catalytiques) qui sont plus efficaces. Effectivement, l’utilisation de ces éponges contre miR-155 résultait en une diminution de la prolifération, de formation de colonie ainsi que de la migration de cellule de myélome multiple. En second lieu, nous nous sommes penchés sur l’étude d’éponges naturelles dans le contexte de la sénescence. Il existe en effet quelques exemples de lARNnc (Long Non-Coding RNA) qui peuvent agir de la sorte pour un miR donné. Nous pensons que c’est par ce mécanisme de régulation que miR-146a, un miR impliqué dans la réponse anti-inflammatoire, peut s’accumuler dans la sénescence induite par RAS sans toutefois sembler être pleinement actif. Effectivement, les cellules sénescentes sécrètent une variété de facteurs pro-inflammatoires. À l’aide d’une nouvelle technique nommée miR-CLIP, nous avons pu étudier l’interactome de miR-146a et avons identifié plusieurs lARNnc qui selon des outils de prédiction, semblent s’hybrider de manière extensive en région 3’ du miR. Ceci est requis pour l’initiation d’un TDMD (Target-Directed miR Degradation) et nous avons donc investigué la possibilité d’un tel évènement dans la régulation de miR-146a. Nos résultats montrent que la surexpression de XXBAC-B444P24.13 mène à une diminution des niveaux de miR-146a qui n’est pas due à une baisse de sa transcription. Bien que le premier article illustre les avantages d’une éponge catalytique artificielle, le second article suggère que cette stratégie pourrait déjà être en place dans les systèmes biologiques, et ce, de manière naturelle. En effet, une fois miR-146a lié à XXBAC-B444P24.13, ce dernier induirait la dégradation du miR par un TDMD. Ceci ouvre donc la porte au développement d’outils qui pourraient être plus performants à des niveaux d’expression plus bas.Cellular senescence is characterized by a cell cycle arrest in the G1/S phase and can be induced by a variety of stresses which include telomere shortening, oncogene activation or oxidative stress. Its establishment is known to require changes in the genetic expression program and non-coding RNA play an important part in this phenomenon. For a long time, this RNA subtype was considered to only be a transcriptional byproduct, but we now know that they are involved in a plethora of functions which are essential to cell homeostasis. Various diseases display aberrant expression of microRNA (miR), small non-coding RNA of 18-22 nucleotides, suggesting they are involved in their development. Such is the case for miR-19 and miR-155, two oncomirs which are found to be overexpressed in hematopoietic cancers. In normal conditions, STAT5A, which is often found dysregulated in those cancers, induces SOCS1 which acts as a retro-inhibitor of this signaling pathway, preventing uncontrolled proliferation. Furthermore, our lab has shown that SOCS1 can also be involved in senescence by facilitating p53 activation. SOCS1 can be targeted by both oncomirs and our results show that artificial sponges, that inhibit miR-19 or miR-155’s functions, lead to the activation of p53. Also, we have incorporated the hammerhead ribozyme in the miR binding sites in the sponge, creating a sponge 2.0 (catalytic sponges). Expressing the latter in a multiple myeloma cell line (RPMI8226) resulted in less proliferation, colony formation and migration. Secondly, we aimed at studying natural sponges in the context of senescence. Indeed, there are quite a few examples of lncRNA (Long Non-Coding RNA) acting as a miRNA inhibitor by quenching them. We think that this mode of regulation could provide an explanation as to how an anti-inflammatory miR, miR-146a, can accumulate in senescence even though it is a pro-inflammatory response. Using a novel technique called miR-CLIP, we were able to study specifically miR-146a’s interactome and have found that it can interact with many lncRNAs. Interestingly, using computational tools, we noticed that miR-146a was predicted to interact with extensive 3’ end hybridization with a number of these lncRNA. This characteristic is known to be required to induce TDMD (Target-Directed miR Degradation). Indeed, when we overexpressed XXBAC-B444P24.13, miR-146a levels went down and this is not caused by a decrease in transcription of the miR. In the first part of this thesis, we show that artificial catalytic sponges have an advantage over a more “classical” design. This is further supported by the fact that this strategy seems to be employed in nature. Indeed, we might have uncovered a lncRNA that when bound to by miR-146a would lead to its degradation using TDMD. This could be taken advantage of in the development of new tools for miR inhibition that would be more powerful and could be potentially used at lower levels of expression

Fugienda petimus : la Phaedra di Seneca come sistema complesso di antitesi

Seneca's Phaedra as a complex system of antithesis.La Phèdre de Sénèque au regard de la tradition antithétique : personnages, espace, temps.La Phaedra di Seneca all'incrocio di tradizioni antitetiche : antitesi spaziali, "naturam sequere" e vita contro natura, conflitto parentale, parola e silenzio

Modular Circular Economy in Energy Infrastructure Projects: Enabling Factors and Barriers

There is a growing body of literature surrounding circular economy (CE) and energy infrastructure projects. Most of this literature focuses on CE initiatives related to material recovering and recycling. The body of knowledge about reusing components is limited and mostly related to the need for reusing rather than providing solutions on how to reuse components. Modularization can be a step towards a solution, enabling entire modules or their components to retain their functionality in other infrastructures. Leveraging 23 semistructured interviews with nuclear and oil and gas experts, mainly based in the UK and US with international experience, this paper deals with the link between modularization and CE (defined modular CE) to identify enabling factors and barriers for the reuse of modules or their components. Relevant enabling factors are the monitoring of module and component conditions, standardization of module and component designs, and early planning. Relevant barriers are the lack of a second-hand market, economics, and regulatory challenges. The results are relevant to the stakeholders involved in planning, building, operating, and decommissioning energy infrastructures

Avaliações morfofisiológicas do feijão pérola irrigado

O objetivo deste trabalho foi avaliar as características morfofisiológicas do feijão Pérola submetido a diferentes lâminas de irrigação na região do Oeste Paulista. O experimento foi conduzido na cidade de Pirapozinho, São Paulo,utilizando vasos de 25 x 32 cm, em condições de campo, no período de março a maio. O delineamento experimental foi em blocos ao acaso, com seis lâminas de irrigação: 25%, 50%, 75%, 100%, 125% e 150% da evapotranspiração da cultura (ETc) e 5 repetições. Os parâmetros avaliados foram altura das plantas, massa seca de parte aérea, massa seca de raiz, número vagens por planta, peso de vagens, número de sementes por vagem, germinação das sementes e determinação do teor de prolina nas folhas. Os dados foram submetidos ao teste F e posteriormente ao teste Tukey para comparação de médias ao nível de probabilidade de 5%. Observou-se que os resultados foram significativos para o peso de vagens, o número de grãos por vagem e teor de prolina. Concluiu-se que a lâmina de irrigação de 75% ETc resultou na maior produção de sementes. A lâmina de irrigação de 25% ETc promoveu déficit hídrico, indicado pela maior concentração de prolina nas folhas frescas.The aimed of this study was to evaluate the physical and physiological characteristics of the Pearl beans under different irrigation levels in Oeste Paulista region. The experiment was conducted in the city of Pirapozinho, Sao Paulo, using potsof 25 x 32 cm, under field conditions, from March to May. The experimental design was a randomized block with six depths of irrigation: 25%, 50 %, 75 %, 100 % , 125 % and 150 % crop evapotranspiration ( ETc ) and 5 replication. The parameters evaluated were plant height, sho ot dry weight , root dry weight, number of pods per plant, pod weight, number of seeds per pod, seed germination and foliar proline. The data were analyzed using the F test and then the Tukey test for comparison of the probability level of 5 %.It was observed that the results were significan t for the weight of the strings, the number of seeds per pod and the concentration of proline. It was concluded that the depth of irrigation of 75 % ETc resulted in higher seed production. The irrigation of 25 % ETc promoted water deficit, indicated by the higher concentration of proline in fresh leaves

Transportation of small modular reactor modules: What do the experts say?

One of the key characteristics of small modular reactors (SMRs), as their name emphasised, is the modularization. Modularization implies factory production, which in turn implies transportation of large, heavy, complex and fragile modules from the factory to the site. Various vendors and organisations are developing several SMR concepts and designs, but there are extremely limited information about the crucial element of modules transportation. Conversely, in other industries (e.g. Oil & Gas), the experience on modules transportation is much greater. This paper provides a structured analysis for the knowledge transfer from the general literature (i.e. other major infrastructure) to the SMR world. Firstly, the paper provides a summary of the literature about transporting large modules. In the second part, the paper presents and discusses the results of a series of interviews with transport industry experts about large modules transportation. The third part provides a summary of the findings and the key takeaways

Developing UK strategy for nuclear SMRs

Nuclear Small Modular Reactors (SMRs) can play a role in UK decarbonisation, providing low-carbon electricity and heat. SMR investments are more affordable and less risky, therefore attracting a wider range of potential investors. The UK government needs to develop a consistent strategy to support UK SMRs