BONEFILL® block as alternative for bone substitute: a toxicological evaluation

Bone substitutes based on hydroxyapatite (HA) and Bonefill® (BO - inorganic bovine bone) associated with poly(lactic-co-glycolic acid) (PLGA) (HA/PLGA and BO/PLGA) were evaluated concerning cytotoxicity, genotoxicity and mutagenicity as potential candidates for bone repair. The materials were developed and provided by Bionnovation Biomedical Products Ltda. Eluates from these bone substitutes were prepared for toxicity evaluations using eukaryotic cell cultures. HA/PLGA was used as a comparison for Bonefill®. Cell viability was evaluated by XTT assay and surviving fraction was calculated for clonogenic survival. Additionally, tail moment was used to assess genotoxicity (comet assay). The frequencies of binucleated cells with micronucleus (FBMN), micronucleus (FMN), nucleoplasmic bridges (NPBs), and nuclear buds (NBUDs) were analysed by cytokinesis-block micronucleus assay (CBMN assay). Results showed no statistical difference in cell viability compared with negative control (NC) The eluates did not promote delayed cytotoxicity whereas the surviving fraction rate for cultured cells was similar to NC. Furthermore, no genotoxicity or mutagenicity effects were observed for cultured cells with the Bonefill/PLGA and HA/PLGA eluates. In conclusion, the negative cytotoxicity, genotoxicity and mutagenicity results indicate that these bone substitutes presented interesting preliminary results as potential biomaterials for bone repair

BONEFILL® block as alternative for bone substitute: a toxicological evaluation

Bone substitutes based on hydroxyapatite (HA) and Bonefill® (BO - inorganic bovine bone) associated with poly(lactic-co-glycolic acid) (PLGA) (HA/PLGA and BO/PLGA) were evaluated concerning cytotoxicity, genotoxicity and mutagenicity as potential candidates for bone repair. The materials were developed and provided by Bionnovation Biomedical Products Ltda. Eluates from these bone substitutes were prepared for toxicity evaluations using eukaryotic cell cultures. HA/PLGA was used as a comparison for Bonefill®. Cell viability was evaluated by XTT assay and surviving fraction was calculated for clonogenic survival. Additionally, tail moment was used to assess genotoxicity (comet assay). The frequencies of binucleated cells with micronucleus (FBMN), micronucleus (FMN), nucleoplasmic bridges (NPBs), and nuclear buds (NBUDs) were analysed by cytokinesis-block micronucleus assay (CBMN assay). Results showed no statistical difference in cell viability compared with negative control (NC) The eluates did not promote delayed cytotoxicity whereas the surviving fraction rate for cultured cells was similar to NC. Furthermore, no genotoxicity or mutagenicity effects were observed for cultured cells with the Bonefill/PLGA and HA/PLGA eluates. In conclusion, the negative cytotoxicity, genotoxicity and mutagenicity results indicate that these bone substitutes presented interesting preliminary results as potential biomaterials for bone repair

RELAÇÃO DOS TRAÇOS DE PERSONALIDADE E DO DESEMPENHO ACADÊMICO NA PERSPECTIVA DA LITERATURA NACIONAL E INTERNACIONAL

Um dos grandes desafios de uma pesquisa científica é a de desenvolver um conhecimento sólido que possa fundamentar e nortear a elaboração de um estudo mais robusto. Assim, buscou-se realizar uma Revisão de Literatura (RL) nacional e internacional sobre os estudos já realizados que contemplassem a relação dos traços de personalidade e o desempenho acadêmico dos estudantes na graduação. A pesquisa define-se como qualitativa, descritiva e exploratória sendo destacado o caráter bidirecional da investigação: i. por meio de buscas no banco digital da Biblioteca Digital de Teses e Dissertações (BDTD) do Instituto Brasileiro de Informação em Ciência e Tecnologia; e, ii. na biblioteca virtual do portal de periódicos da Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), na Comunidade Acadêmica Federada (CAFe). Na análise nacional, observou-se que a temática “Traços de Personalidade” não se encontrava no escopo geral de nenhuma das teses e, na busca internacional, observou-se apenas estudos iniciais de personalidade relacionados ao desempenho em nível superior. Isto posto, a literatura não é conclusiva sobre a influência dos traços de personalidade no desempenho acadêmico a nível superior, embora tenha-se ampliada a percepção da influência que possuem os traços de personalidade nos resultados acadêmicos dos estudantes

Papilomatose de laringe

Laryngeal papillomatosis is the most frequent benign neoplasm of the larynx, occurring in children and adults. It is caused by the human papilloma virus, especially types 6 and 11. The clinical course is unpredictable and recurrence is frequent in children. There have been reports of association between this disease and tracheobronchial tree and squamous cell carcinoma of the larynx. Papillomatosis is probably one of thefactors that can predispose a patient to the development of cancer, similarly to alcohol, tobacco, and radiation exposure.Treatment aims at maintaining airway patency and speech. Surgery with carbon dioxide laser is able to achieve this objective, however, without preventing recidivation. The low rates of surgical cure have been stimulating new research with chemotherapics. Unfortunately these have not been successful. Their main purpose is to reduce the extension of the disease and rates of recidivation, slowing down papilloma growth.The present study reviews pathophysiology, the clinical presentation and treatment aspects .A papilomatose de laringe é a neoplasia benigna que mais freqüentemente acomete a laringe, tanto em crianças quanto em adultos. É causada pelo papiloma vírus humano, em especial pelos tipos 6 e 11. A apresentação clínica é variável e a evolução praticamente imprevisível, devido especialmente ao alto grau de recorrência típico desta entidade. Existem diversos relatos que demonstram associação entre estadoença e o carcinoma de células escamosas de laringe e de árvore traqueobrônquica. Acredita-se que a papilomatose seja um dos fatores que predisponham o paciente ao câncer, assim como o são o álcool, o fumo e a exposição à radiação ionizante. O objetivo do tratamento é a manutenção da via aérea e da função vocal. Para tanto, a microcirurgia com laser tem demonstrado os melhores resultados, apesar de nãoimpedir as recorrências. Assim, os pobres índices de cura cirúrgica têm estimulado o desenvolvimento de estudos com quimioterápicos. A proposta do uso destes é reduzir a extensão da doença e a freqüência das recidivas, lentificando o crescimento do papiloma. Essas terapias adjuvantes, entretanto, ainda apresentam pouco sucesso a longo prazo. Realizamos aqui uma revisão da literatura a respeito do assunto, com ênfase nos aspectos de fisiopatologia, apresentação clínica e tratamento