Genetic Diversity in Relict and Fragmented Populations of Ulmus glabra Hudson in the Central System of the Iberian Peninsula

Ulmus glabra Hudson, or Wych elm, occurs as fragmented and relict natural populations in the Central System, which acts as a refugium in the Iberian Peninsula. Considering the importance of the Central System populations of U. glabra, the main objective was to assess their genetic diversity using nuclear microsatellite markers. A total of 360 different genotypes were detected in the 427 U. glabra individuals analyzed. Wych elm populations showed a highly significant genetic differentiation (24%; p = 0.0001). Of the 22 populations studied, population of Rozas de Puerto Real (ROZ) showed the highest values of effective number of alleles (2.803), mean Shannon?s diversity (1.047) and expected heterozygosity (0.590). Populations of ROZ and Mombeltrán (MOM) showed the highest values of observed heterozygosity (0.838 and 0.709, respectively), and highly negative values for inbreeding coefficient (?0.412 and ?0.575, respectively). Also, most of putative hybrids (50 of 55) were observed in these two populations. Demographic analysis revealed signals for recent (four populations) and ancestral (fifteen populations) bottlenecks. Fragmented populations with diminishing number of individuals, along with anthropogenic intervention and Dutch elm disease (DED), are the main threats to U. glabra populations. From a future perspective, the information generated can be considered in the formulation of conservation strategies for U. glabra populations in the Central System

An Alternative Approach to Obtain a New Gain in Step-Size of LMS Filters Dealing with Periodic Signals

Partial updates (PU) of adaptive filters have been successfully applied in different contexts to lower the computational costs of many control systems. In a PU adaptive algorithm, only a fraction of the coefficients is updated per iteration. Particularly, this idea has been proved as a valid strategy in the active control of periodic noise consisting of a sum of harmonics. The convergence analysis carried out here is based on the periodic nature of the input signal, which makes it possible to formulate the adaptive process with a matrix-based approach, the periodic least-mean-square (P-LMS) algorithm In this paper, we obtain the upper bound that limits the step-size parameter of the sequential PU P-LMS algorithm and compare it to the bound of the full-update P-LMS algorithm. Thus, the limiting value for the step-size parameter is expressed in terms of the step-size gain of the PU algorithm. This gain in step-size is the quotient between the upper bounds ensuring convergence in the following two scenarios: first, when PU are carried out and, second, when every coefficient is updated during every cycle. This step-size gain gives the factor by which the step-size can be multiplied so as to compensate for the convergence speed reduction of the sequential PU algorithm, which is an inherently slower strategy. Results are compared with previous results based on the standard sequential PU LMS formulation. Frequency-dependent notches in the step-size gain are not present with the matrix-based formulation of the P-LMS. Simulated results confirm the expected behavior

Novel reaction force for ultra-relativistic dynamics of a classical point charge

The problem of the electromagnetic radiation of an accelerated charged particle is one of the most controversial issues in Physics since the beginning of the last century, representing one of the most popular unsolved problems of the Modern Physics. Different equations of motion have been proposed throughout history for a point charge including the electromagnetic radiation emitted, but all these expressions show some limitations. An equation based on the principle of conservation of energy is proposed in this work for the ultra-relativistic motion. Different examples are analyzed showing that the energy lost by the charge agrees with the Li\'enard formula. This proposed equation has been compared with the Landau-Lifshitz equation obtaining a good agreement in the range of application of the Landau-Lifshitz formula.Comment: 9 pages, 10 figure

Numerical study of dark current dynamics in a high-gradient backward travelling wave accelerating cavity using the electromagnetic simulation software CST studio.

High-Gradient accelerating cavities are one of the main research lines in the development of compact linear colliders. However, the operation of such cavities is currently limited by nonlinear effects that are intensified at high electric fields, such as dark currents and radiation emission or RF breakdowns. A new normal-conducting High-Gradient S-band Backward Travelling Wave accelerating cavity for medical application (v=0.38c) designed and constructed at Conseil Européen pour la Recherche Nucléaire (CERN) is being tested at Instituto de Física Corpuscular (IFIC) High Power RF Laboratory. The objective consists of studying its viability in the development of compact linear accelerators for hadrontherapy treatments in hospitals. Due to the high surface electric field in the cavity, electrons are emitted following Fowler- Nordheim equation, also known as dark currents. The emission and dynamic of these electrons are of fundamental importance on different phenomena such as RF Breakdowns or radiation dose emission. In this work, 3D electromagnetic numerical simulations have been performed using the computer simulation technology software CST Studio Suite. Then, the resulting EM field maps are used to study the emission and electron dynamics inside the cavity. The simulation results are compared with experimental data and first conclusions discussed

Relativistic particle motion of a charge including the radiation reaction

The problem of the electromagnetic radiation of an accelerated charged particle is one of the most controversial issues in Physics since the beginning of the last century representing one of the most popular unsolved problems of the Modern Physics. Different equations of motion for a point charge including the electromagnetic radiation emitted have been proposed throughout history, but all these expressions show some limitations. An equation based on the principle of conservation of energy is proposed for the ultra-relativistic motion. Different examples are analyzed showing that the energy lost by the charge agrees with the relativistic generalization of the Larmor formula. This proposed equation has been compared with the Landau-Lifshitz equation obtaining a good agreement in the range of application of the Landau-Lifshitz formula. Finally, it is discussed a possible variation of the typical relativistic particle integrators (e.g. Boris, Vay or Higuera-Cary methods) in order to include the radiation reaction

Study of the RF pulse heating phenomenon in high gradient accelerating devices by means of analytical approximations

The main objective of this work is to present a simple method, based on analytical expressions, for obtaining a quick approximation of the temperature rise due to the Joule effect inside the metallic walls of an RF accelerating device. This proposal relies on solving the 1D heat-transfer equation for a thick wall, where the heat sources inside the wall are the ohmic losses produced by the RF electromagnetic fields penetrating the metal with finite electrical conductivity. Furthermore, it is discussed how the theoretical expressions of this method can be applied to obtain an approximation to the temperature increase in realistic 3D RF accelerating structures, taking as an example the cavity of an RF electron gun. These theoretical results have been benchmarked with numerical simulations carried out with commercial finite-element method codes, finding good agreement among them

The role of emotional intelligence and self-care in the stress perception during COVID-19 outbreak: An intercultural moderated mediation analysis

Background: The relationship between emotional intelligence (EI) and stress has been widely studied, as well as the beneficial role of self-care to maintain health and wellbeing. However, the joint contribution of EI and self- care in predicting stress has not been examined during COVID-19 lockdown. This study aimed to examine the mediating role of self-care in the relationship between EI and stress and the potential moderator role of gender. Methods: A sample of 1082 participants from four Hispanic countries completed measures related to socio- demographic, trait emotional intelligence (Trait Meta-Mood Scale), self-care activities (Self-care Activities Screening Scale) and stress (Perceived Stress Scale). Results: Mediation analyses revealed that self-care increased the explained variance of the prediction of stress by EI dimensions after controlling gender and age. However, gender only moderated the relationship between selfcare and stress in the mediation model corresponding to emotional attention. Conclusions: Data supported a general model for the interaction of EI and self-care as contributing factors of stress. Further research is needed to replicate it in more culturally distant samples and to fully explore the po- tential role of gender differences. Future intervention programs should include a balanced combination of EI and self-care to increase their benefits on people’s healt

Two-dimensional simulation of the electron transport in a photomultiplier tube

Photomultiplier tubes are widely used in experimental physics because they convert small light signals into a measurable electric current. Although their working principle is well known, it is very difficult to find simulations of the electron transport in these devices. For this reason, the electron transport in the Hamamatsu R13408-100 photomultiplier tube has been simulated in 2D. The software SUPERFISH is used for calculating the electrostatic fields and the Boris method for the effective electron dynamics. The secondary electron emission in the dynodes is implemented using an effective electron model and the modified Vaughan’s model. Some figures of merit for photomultiplier tubes (e.g. the gain, the electron transit time or the transit time spread) in function of the supply voltage and an external magnetic field have been studied obtaining a good qualitative accordance with the Hamamatsu datasheet. In further studies, we are going to compare our simulations with experimental measurements

TTBK1 and CK1 inhibitors restore TDP-43 pathology and avoid disease propagation in lymphoblast from Alzheimer’s disease patients

IntroductionTDP-43 proteinopathy in Alzheimer’s disease (AD) patients is recently emerging as a relevant pathomolecular event that may have been overlooked. Recent results in immortalized lymphocytes from AD patients have shown not only an increase of post-translational modifications in TDP-43, such as hyperphosphorylation and fragmentation, but also its prionic behaviour and cell-to-cell disease transmission. With the main goal to advance therapeutic interventions, we present in this work different kinase inhibitors with potential to restore this pathological mechanism.MethodologyWe have used immortalized lymphocytes from healthy controls and AD severe patients to evaluate the correction of TDP-43 pathology after the treatment with previously synthetized TTBK1 and CK1 inhibitors. Moreover we used the conditioned mediums of these cells to perform different disease propagation experiments.ResultsTDP-43 pathology observed in lymphoblasts from severe AD patients is reduced after the treatment with TTBK1 and CK1 inhibitors (decreasing phosphorylation and increasing nuclear localisation), Furthermore, the significant increase in TDP-43 phosphorylation, cytoplasmic accumulation and aberrant F-actin protrusions (TNT-like structures) observed in control cells growing in CM from AD lymphoblasts were abolished when the CM from AD lymphoblasts treated with previously reported TTBK1 and CK1 inhibitors were used. In addition, the cytosolic transport mediated by molecular motors of the receptor cells was altered with the induced TDP-43 pathology, but it was not produced with the abovementioned pretreated CMs.ConclusionTTBK1 and CK1 inhibitors, specially VNG1.47 and IGS2.7 compounds, restore TDP-43 pathology and avoid cell-to-cell propagation in immortalized lymphocytes from AD patients, being excellent candidates for the future therapy of this prevalent and devastating disease

Nitrogen-doped carbon nanodots deposited on titania nanoparticles: unconventional near-infrared active photocatalysts for cancer therapy

Cancer represents a major public health issue and a primary cause of death for the mankind and the search for alternative cancer treatments that assist or alleviate the drawbacks of current cancer therapies remains imperative. Nanocatalytic medicine represents a new discipline that aims at exploiting the unique response of heterogeneous catalysts exposed to unconventional conditions such as those encountered in the tumor microenvironment (TME). Photo-triggered cancer therapies using light-activable catalytic materiales can stimulate and activate multiple biological processes and represent a very promising field of study. Herein, we evaluate the use of carbon nanodots with different composition (CNDs) retrieved by laser pyrolysis as potential near-infrared (NIR) photosensitizers able to activate P25 semiconductor nanostructured photocatalysts. We describe the enhanced photocatalytic response towards glucose conversion and reactive oxygen species (ROS) generation upon irradiation with NIR-LEDs when CNDs doped with heteroatoms were tested. The most active photocatalysts were evaluated in the presence of cancer cells and revealed a promising photodynamic effect under NIR irradiation. This work represents one of the scarce examples of a conventional inorganic photocatalyst containing TiO2 that is translated into a biomedical application with a successful outcome