Urban water security: a comparative study of cities in the arid Americas

In this study we analyse how three cities in the arid Americas have addressed urban growth while facing water scarcity: Hermosillo, Mexico; Mendoza, Argentina; and Tucson, USA. We use the urban water security framework to examine five domains of water management: sociodemographic, economic, technological, ecological and governance (SETEG). Our analysis indicates that, in spite of water scarcity, urban growth has been promoted in the three cities. We argue that this expansion, although encouraged for economic development, is not sustainable in the long term. In the three cities, groundwater plays a major role in water supply, but growth has negatively affected riparian ecosystems, the health of the aquifers and access to domestic water. In order to pursue water security, several options are essential to enhance social?ecological system resilience. These include limits or reduction of urban expansion, reuse of treated effluent for riparian conservation, and community conservation efforts such as rainwater harvesting and other green infrastructure practices.Fil: Díaz Caravantes, Rolando E.. El Colegio de Sonora; MéxicoFil: Zuniga Teran, Adriana. University of Arizona; Estados UnidosFil: Martin, Facundo Damian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Ciencias Humanas, Sociales y Ambientales; ArgentinaFil: Bernabeu, María Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Ciencias Humanas, Sociales y Ambientales; ArgentinaFil: Stoker, Philip. University of Arizona; Estados UnidosFil: Scott, Christopher. University of Arizona; Estados Unido

Quantification of HIV-1 RNA Among Men Who Have Sex With Men Using an At-Home Self-Collected Dried Blood Spot Specimen: Feasibility Study

Background: Suboptimal antiretroviral therapy (ART) adherence and disengagement in care present significant public health challenges because of the increased probability of HIV transmission. In the United States, men who have sex with men (MSM) continue to be disproportionately affected by HIV, highlighting a critical need to engage high-risk MSM living with HIV who are not engaged or retained in care. Objective: The aim of the study was to assess the feasibility of at-home blood self-collection and laboratory quantification of HIV-1 RNA viral load (VL) to report laboratory-based VL outcomes and compare self-reported and laboratory-reported VL Methods: Between 2016 and 2017, 766 US HIV-positive MSM enrolled in a Web-based behavioral intervention were invited to participate in an at-home dried blood spot (DBS) collection study using HemaSpot-HF kits (Spot On Sciences, Inc, Austin, TX) for laboratory-quantified VL. Results: Of those invited to participate, 72.3% (554/766) enrolled in the DBS study. Most (79.2%, 439/554) men enrolled reported attempting to collect their blood, 75.5% (418/554) of participants mailed a DBS specimen to the research laboratory, and 60.8% (337/554) had an adequate blood sample for VL testing. Of the 337 specimens tested for VL by the laboratory, 52.5% (177/337) had detectable VL (median: 3508 copies/mL; range: 851-1,202,265 copies/mL). Most men (83.9%, 135/161) who returned a DBS specimen with laboratory-quantified detectable VL self-reported an undetectable VL during their last clinical visit. Conclusions: Home collection of DBS samples from HIV-positive MSM is feasible and has the potential to support clinical VL monitoring. Discrepant laboratory HIV-1 RNA values and self-reported VL indicate a need to address perceived VL status, especially in the era of treatment as prevention. Most participants were willing to use an at-home DBS kit in the future, signaling an opportunity to engage high-risk MSM in long-term HIV care activities

Characterization of Intact Proviruses in Blood and Lymph Node from HIV-Infected Individuals Undergoing Analytical Treatment Interruption.

The role of lymphoid tissue as a potential source of HIV-1 rebound following interruption of antiretroviral therapy (ART) is uncertain. To address this issue, we compared the latent viruses obtained from CD4+ T cells in peripheral blood and lymph nodes to viruses emerging during treatment interruption. Latent viruses were characterized by sequencing near-full-length (NFL) proviral DNA and env from viral outgrowth assays (VOAs). Five HIV-1-infected individuals on ART were studied, four of whom participated in a clinical trial of a TLR9 agonist that included an analytical treatment interruption. We found that 98% of intact or replication-competent clonal sequences overlapped between blood and lymph node. In contrast, there was no overlap between 205 latent reservoir and 125 rebound sequences in the four individuals who underwent treatment interruption. However, rebound viruses could be accounted for by recombination. The data suggest that CD4+ T cells carrying latent viruses circulate between blood and lymphoid tissues in individuals on ART and support the idea that recombination may play a role in the emergence of rebound viremia.IMPORTANCE HIV-1 persists as a latent infection in CD4+ T cells that can be found in lymphoid tissues in infected individuals during ART. However, the importance of this tissue reservoir and its contribution to viral rebound upon ART interruption are not clear. In this study, we sought to compare latent HIV-1 from blood and lymph node CD4+ T cells from five HIV-1-infected individuals. Further, we analyzed the contribution of lymph node viruses to viral rebound. We observed that the frequencies of intact proviruses were the same in blood and lymph node. Moreover, expanded clones of T cells bearing identical proviruses were found in blood and lymph node. These latent reservoir sequences did not appear to be the direct origin of rebound virus. Instead, latent proviruses were found to contribute to the rebound compartment by recombination

Assessment of sorption capability of montmorillonite clay for lead removal from water using laser–induced breakdown spectroscopy and atomic absorption spectroscopy

Laser-induced breakdown spectroscopy (LIBS) and atomic absorption spectroscopy (AAS) techniques were applied for quantitative analysis of the remaining Pb content in water samples after treatment with a raw montmorillonite (MMT) and an organic derivative (MMO) clays for heavy metal removal, an issue of crucial importance for decontamination of water bodies. The Pb sorption capabilities of MMT and MMO clays were assessed by using solutions with known Pb concentrations, in the range 100-500 ppm. To carry out the LIBS analysis, the samples were prepared in the form of solid pellets with powdered calcium hydroxide addition. The measurement conditions were optimized to achieve reliable analytical results and the plasma parameters, i.e., temperature and electron density, were obtained. For quantification purpose, a calibration curve was constructed with the Pb I emission line at 4057.8 Å measured at the time window 30-45 μs by using reference samples with Pb concentrations in the range 56-715 ppm. The Pb residual content in the liquid samples determined with LIBS showed a good agreement with those measured with AAS. The sorption efficiency of Pb from contaminated water was calculated through a sorption percentage SPb%. The calculated SPb% was higher for MMT (56%-100%) than for MMO (27%-47%). The results also demonstrated the usefulness of LIBS method for the determination of Pb concentrations in liquid samples.Fil: Teran, Ezequiel Jesús. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigaciones en Física e Ingeniería del Centro de la Provincia de Buenos Aires. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigaciones en Física e Ingeniería del Centro de la Provincia de Buenos Aires. - Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigaciones en Física e Ingeniería del Centro de la Provincia de Buenos Aires; ArgentinaFil: Montes, María Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Física La Plata. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Física La Plata; ArgentinaFil: Rodriguez, Corina Iris. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Humanas. Departamento de Ciencias Ambientales. Centro de Investigación y Estudios Ambientales; ArgentinaFil: Martino, Lucila Juliana. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigaciones en Física e Ingeniería del Centro de la Provincia de Buenos Aires. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigaciones en Física e Ingeniería del Centro de la Provincia de Buenos Aires. - Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigaciones en Física e Ingeniería del Centro de la Provincia de Buenos Aires; ArgentinaFil: Quiroga, Miguel Angel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Landa, Roberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Torres Sanchez, Rosa Maria. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Tecnología de Recursos Minerales y Cerámica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Tecnología de Recursos Minerales y Cerámica; ArgentinaFil: Diaz Pace, Diego Martin. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigaciones en Física e Ingeniería del Centro de la Provincia de Buenos Aires. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigaciones en Física e Ingeniería del Centro de la Provincia de Buenos Aires. - Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigaciones en Física e Ingeniería del Centro de la Provincia de Buenos Aires; Argentin

Eukaryotic elongation factor 2 controls TNF-alpha translation in LPS-induced hepatitis

Bacterial LPS (endotoxin) has been implicated in the pathogenesis of acute liver disease through its induction of the proinflammatory cytokine TNF-alpha. TNF-alpha is a key determinant of the outcome in a well-established mouse model of acute liver failure during septic shock. One possible mechanism for regulating TNF-alpha expression is through the control of protein elongation during translation, which would allow rapid cell adaptation to physiological changes. However, the regulation of translational elongation is poorly understood. We found that expression of p38gamma/delta MAPK proteins is required for the elongation of nascent TNF-alpha protein in macrophages. The MKK3/6-p38gamma/delta pathway mediated an inhibitory phosphorylation of eukaryotic elongation factor 2 (eEF2) kinase, which in turn promoted eEF2 activation (dephosphorylation) and subsequent TNF-alpha elongation. These results identify a new signaling pathway that regulates TNF-alpha production in LPS-induced liver damage and suggest potential cell-specific therapeutic targets for liver diseases in which TNF-alpha production is involved

Highly asymmetric magnetic behavior in exchange biased systems induced by noncollinear field cooling

This article may be downloaded for personal use only. Any other use requires prior permission of the author and the American Institute of Physics.A detailed study of the angular dependence of the magnetization reversal in polycrystalline ferromagnetic (FM)/antiferromagnetic Co/IrMn bilayers with noncollinear FM and unidirectional anisotropies shows a peculiar asymmetric magnetic behavior. The anisotropy configuration is set via a field cooling (FC) procedure with the magnetic field misaligned with respect to the easy magnetization direction of the FM layer. Different magnetization reversal modes are observed for either positive or negative angles with respect to the FC direction. The angular dependence of both coercivity and exchange bias also clearly displays the broken symmetry of the induced noncollinearity. Our findings are reproduced with a modified Stoner-Wohlfarth model including the induced anisotropy configuration. Our results highlight the importance of the relative angle between anisotropies in exchange bias systems, opening a new path for the tailoring of their magnetic properties

Dialogic science-policy networks for water security governance in the arid Americas

Addressing wicked problems challenging water security requires participation from multiple stakeholders, often with conflicting visions, complicating the attainment of water-security goals and heightening the need for integrative and effective science-policy interfaces. Sustained multi-stakeholder dialogues within science-policy networks can improve adaptive governance and water system resilience. This paper describes what we define as “dialogic science-policy networks,” or interactions -- both in structural and procedural terms -- between scientists and policy-makers that are: 1) interdisciplinary, 2) international (here, inter-American), 3) cross-sectoral, 4) open, 5) continual and iterative in the long-term, and 6) flexible. By fostering these types of interactions, dialogic networks achieve what we call the 4-I criteria for effective science-policy dialogues: inclusivity, involvement, interaction, and influence. Here we present several water-security research and action projects where some of these attributes may be present. Among these, a more comprehensive form of a dialogic network was intentionally created via AQUASEC, a virtual center and network initially fostered by a series of grants from the Inter-American Institute for Global Change Research. Subsequently, AQUASEC has significantly expanded to other regions through direct linkages and additional program support for the International Water Security Network, supported by Lloyd's Register Foundation and other sources. This paper highlights major scientific and policy achievements of a notable suite of science-policy networks, shared practices, methods, and knowledge integrating science and policy, as well as the main barriers overcome in network development. An important gap that remains for future research is the assessment and evaluation of dialogic science-policy networks' long-term outcomes.Fil: Lutz Ley, America N.. El Colegio de Sonora; MéxicoFil: Scott, Christopher A.. University of Arizona; Estados UnidosFil: Wilder, Margaret. University of Arizona; Estados UnidosFil: Varady, Robert G.. University of Arizona; Estados UnidosFil: Ocampo Melgar, Anahi. Universidad de Chile.; ChileFil: Lara Valencia, Francisco. Arizona State University; Estados UnidosFil: Zuniga Teran, Adriana. University of Arizona; Estados UnidosFil: Buechler, Stephanie. University of Arizona; Estados UnidosFil: Díaz Caravantes, Rolando. El Colegio de Sonora; MéxicoFil: Ribeiro Neto, Alfredo. El Colegio de Sonora; MéxicoFil: Pineda Pablos, Nicólas. El Colegio de Sonora; MéxicoFil: Martin, Facundo Damian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Ciencias Humanas, Sociales y Ambientales; Argentin

Índice de progreso social del distrito de Yonán - Cajamarca

El propósito de la presente investigación es la de obtener el cálculo del índice de progreso social del distrito de Yonán de la provincia de Contumazá ubicada en el departamento de Cajamarca, mediante la metodología elaborada por el Social Progress Imperative, la cual se basa en la integración de tres dimensiones: necesidades humanas básicas, fundamentos de bienestar y oportunidades. La presente investigación es del tipo cuantitativa no experimental, en la cual la recolección de los datos primarios se obtuvo mediante encuestas realizadas a una muestra de la población del distrito de Yonán, el cuestionario empleado fue proporcionado por CENTRUM PUCP el cual es una adaptación del cuestionario del Social Progress Imperative a la realidad nacional. El resultado del IPS del distrito de Yonán resultó 51.2, el cual se encuentra en un nivel bajo según la escala de niveles proporcionado en el estudio del Índice de Progreso Social Regional elaborado por CENTRUM PUCP en el 2019. Sin embargo, este resultado es superior al del departamento de Cajamarca el cual tiene un IPS de 49. En relación a las tres dimensiones las puntuaciones fueron: necesidades humanas básicas con 61.32 en un nivel medio bajo, fundamentos de bienestar con 39.80 en un nivel muy bajo, y oportunidades con 52.36 con un nivel bajo. El distrito de Yonán fue dividido en tres zonas, de las cuales la zona 2, que corresponden a la capital del distrito fue la que presentó mejores resultados en las tres dimensiones analizadas. Los resultados de esta investigación nos muestran las carencias existentes en las diferentes dimensiones mostradas que sirven como fuente de información o punto de partida para dirigir los esfuerzos y políticas municipales en atender las brechas identificadas para lograr el desarrollo y una mejor calidad de vida de los habitantes del distrito de Yonán.The purpose of this research is to obtain the calculation of the social progress index of the Yonán district of the province of Contumazá located in the department of Cajamarca, through the methodology developed by the Social Progress Imperative, which is based on integration of three dimensions: Basic human needs, foundations of well-being, and opportunities. This research is of the non-experimental quantitative type, in which the collection of primary data was obtained through surveys carried out on a sample of the population of the Yonán district, the questionnaire used was provided by CENTRUM PUCP which is an adaptation of the questionnaire from the Social Progress Imperative to the national reality. The result of the IPS of the Yonán district was 51.2, which is at a low level according to the scale of levels provided in the study of the Regional Social Progress Index prepared by CENTRUM PUCP in 2019. However, this result is higher than from the department of Cajamarca, which has an IPS of 49. In relation to the three dimensions, the scores were: basic human needs with 61.32 at a medium-low level, well-being fundamentals with 39.80 at a very low level, and opportunities with 52.36 with a low level. The Yonán district was divided into three zones, of which zone 2, corresponding to the district capital, was the one that presented the best results in the three dimensions analyzed. The results of this research show us the existing deficiencies in the different dimensions shown that serve as a source of information or a starting point to direct municipal efforts and policies to address the identified gaps to achieve development and a better quality of life of the inhabitants Yonán district