Mapping the more highlights items by Tripadvisor comments when choosing a hotel in Seville

Tripadvisor se ha convertido en una fuente de información primordial a la hora de elegir un hotel. De esta forma, los comentarios vertidos en su web pasan a ser un factor determinante para muchos usuarios en la selección de su alojamiento, hecho al que no permanece ajeno el empresario hotelero. Por tanto, estudios como el que aquí se presentan ayudan a identificar cuáles son los ítems más valorados por el cliente, haciéndole experimentar una estancia diez. Así pues, en esta investigación se analiza los comentarios calificados como excelente en los hoteles de 4 y 5 estrellas de Sevilla, de tal forma que los resultados determinan la ubicación, los servicios, las instalaciones y las habitaciones como los aspectos más valorados.Tripadvisor has become a prime source of information when choosing a hotel. Thus, the comments in the Tripadvisor web become a determining factor for many users in selecting their accommodation, a fact that hoteliers cannot ignore. Therefore, studies such as presented here help to identify the most valued items by clients, making their stay experience as perfect. Hence, in this research the comments rated as excellent in 4 and 5 stars’ hotels in Seville are analyzed, so the results determine that location, services, facilities and rooms as the most valued aspects

The perfect stay in 4 and 5 star hotels in Seville through the analysis of comments on TripAdvisor. Determination of the main items

Si en algo coinciden hoy en día la mayor parte de los empresarios hoteleros es que las opiniones online realizadas por los usuarios turísticos suponen una gran fuente de información para la oferta turística. Por ello, se cree necesario analizar cuáles son los principales ítems valorados para considerar una estancia perfecta en hoteles de cuatro y cinco estrellas de la ciudad de Sevilla. Para ello, se utilizará como base de datos la web de viajes TripAdvisor, y como herramienta de análisis cualitativo el software NVivo10. Los datos obtenidos apuntan a que los ítems más valorados son la ubicación, los servicios ofrecidos y las instalaciones, las habitaciones, el trato y la eficacia del personal, el precio, la calidad y la limpieza.Today, if anything matches most hoteliers is that online tourist opinions made by users are a great source of information for tourism. Therefore, it is believed that it is necessary to analyze what are the main appreciated items in order to consider a perfect stay in hotels of four and five stars in the city of Seville. To do this, the TrivAdvisor web will be used as a database and the NVivo10 software as a qualitative analysis tool. The data obtained suggest the most valued items are location, services and facilities, as well as rooms, dealing and staff efficiency, price, quality and cleanliness

Pseudomonas aeruginosa That Specifically Mediates Chemotaxis Toward α-Ketoglutarate

Pseudomonas aeruginosa is an ubiquitous pathogen able to infect humans, animals, and plants. Chemotaxis was found to be associated with the virulence of this and other pathogens. Although established as a model for chemotaxis research, the majority of the 26 P. aeruginosa chemoreceptors remain functionally un-annotated. We report here the identification of PA5072 (named McpK) as chemoreceptor for α-ketoglutarate (αKG). High-throughput thermal shift assays and isothermal titration calorimetry studies (ITC) of the recombinant McpK ligand binding domain (LBD) showed that it recognizes exclusively α-ketoglutarate. The ITC analysis indicated that the ligand bound with positive cooperativity (Kd1 = 301 μM, Kd2 = 81 μM). McpK is predicted to possess a helical bimodular (HBM) type of LBD and this and other studies suggest that this domain type may be associated with the recognition of organic acids. Analytical ultracentrifugation (AUC) studies revealed that McpK-LBD is present in monomer-dimer equilibrium. Alpha-KG binding stabilized the dimer and dimer self-dissociation constants of 55 μM and 5.9 μM were derived for ligand-free and αKG-bound forms of McpK-LBD, respectively. Ligand-induced LBD dimer stabilization has been observed for other HBM domain containing receptors and may correspond to a general mechanism of this protein family. Quantitative capillary chemotaxis assays demonstrated that P. aeruginosa showed chemotaxis to a broad range of αKG concentrations with maximal responses at 500 μM. Deletion of the mcpK gene reduced chemotaxis over the entire concentration range to close to background levels and wild type like chemotaxis was recovered following complementation. Real-time PCR studies indicated that the presence of αKG does not modulate mcpK expression. Since αKG is present in plant root exudates it was investigated whether the deletion of mcpK altered maize root colonization. However, no significant changes with respect to the wild type strain were observed. The existence of a chemoreceptor specific for αKG may be due to its central metabolic role as well as to its function as signaling molecule. This work expands the range of known chemoreceptor types and underlines the important physiological role of chemotaxis toward tricarboxylic acid cycle intermediates. [EN]FEDER funds and Fondo Social Europeo through grants from the Junta de Andalucía (grant CVI-7335) and the Spanish Ministry for Economy and Competitiveness (grant BIO2013-42297). MM was supported by the Spanish Ministry of Economy and Competitiveness Postdoctoral Research Program, Juan de la Cierva (JCI-2012-11815).Peer reviewe

Determination of Ligand Profiles for Pseudomonas aeruginosa Solute Binding Proteins

Solute binding proteins (SBPs) form a heterogeneous protein family that is found in all kingdoms of life. In bacteria, the ligand-loaded forms bind to transmembrane transporters providing the substrate. We present here the SBP repertoire of Pseudomonas aeruginosa PAO1 that is composed of 98 proteins. Bioinformatic predictions indicate that many of these proteins have a redundant ligand profile such as 27 SBPs for proteinogenic amino acids, 13 proteins for spermidine/putrescine, or 9 proteins for quaternary amines. To assess the precision of these bioinformatic predictions, we have purified 17 SBPs that were subsequently submitted to high-throughput ligand screening approaches followed by isothermal titration calorimetry studies, resulting in the identification of ligands for 15 of them. Experimentation revealed that PA0222 was specific for y-aminobutyrate (GABA), DppA2 for tripeptides, DppA3 for dipeptides, CysP for thiosulphate, OpuCC for betaine, and AotJ for arginine. Furthermore, RbsB bound D-ribose and D-allose, ModA bound molybdate, tungstate, and chromate, whereas AatJ recognized aspartate and glutamate. The majority of experimentally identified ligands were found to be chemoattractants. Data show that the ligand class recognized by SPBs can be predicted with confidence using bioinformatic methods, but experimental work is necessary to identify the precise ligand profile.This work was supported by FEDER funds and Fondo Social Europeo through a grant from the Spanish Ministry for Economy and Competitiveness to T. Krell (BIO2016-76779-P). This funding source was not involved in the design and conduct of this stud

High-affinity chemotaxis to histamine of the human pathogen Pseudomonas aeruginosa

FEMS 2019: 8th congress of European Microbiologists, in Glasgow, Scotland, 7-11 July 201

Valoración del cambio de composición corporal en adolescentes con sobrepeso y obesidad incluidos en un tratamiento multidisciplinar: Estudio EVASYON

La adolescencia se considera una etapa vulnerable desde el punto de vista nutricional. La obesidad en la infancia y adolescencia se considera una enfermedad compleja, que preocupa tanto a padres como a profesionales sanitaros debido a su alta prevalencia y a sus consecuencias en la edad adulta. Por ello, los objetivos generales de este trabajo son: 1) valorar los índices antropométricos sencillos como predictores de los cambios de grasa corporal, empleando absorciometría dual de rayos X como método de referencia; 2) identificar los predictores cognitivos y conductuales del abandono de un estudio de intervención, y calcular la tasa de abandono; 3) valorar los cambios de composición corporal en niños y adolescentes, empleando diferentes métodos de composición corporal; y 4) valorar si el cumplimiento de la intervención dietética y el índice de calidad de la dieta son predictores de los cambios de composición corporal durante un seguimiento de 13 meses de una intervención multidisciplinar..

Valoración del cambio de composición corporal en adolescentes con sobrepeso y obesidad incluidos en un tratamiento multidisciplinar: Estudio EVASYON

II Congreso de Alimentación, Nutrición y Dietética. Avances en Nutrición y Dietética Clínica: Prevención, Tratamiento y Gestión - Rol del Dietista-Nutricionist

COVID-19 Severity and Survival over Time in Patients with Hematologic Malignancies: A Population-Based Registry Study

Mortality rates for COVID-19 have declined over time in the general population, but data in patients with hematologic malignancies are contradictory. We identified independent prognostic factors for COVID-19 severity and survival in unvaccinated patients with hematologic malignancies, compared mortality rates over time and versus non-cancer inpatients, and investigated post COVID-19 condition. Data were analyzed from 1166 consecutive, eligible patients with hematologic malignancies from the population-based HEMATO-MADRID registry, Spain, with COVID-19 prior to vaccination roll-out, stratified into early (February–June 2020; n = 769 (66%)) and later (July 2020–February 2021; n = 397 (34%)) cohorts. Propensity-score matched non-cancer patients were identified from the SEMI-COVID registry. A lower proportion of patients were hospitalized in the later waves (54.2%) compared to the earlier (88.6%), OR 0.15, 95%CI 0.11–0.20. The proportion of hospitalized patients admitted to the ICU was higher in the later cohort (103/215, 47.9%) compared with the early cohort (170/681, 25.0%, 2.77; 2.01–3.82). The reduced 30-day mortality between early and later cohorts of non-cancer inpatients (29.6% vs. 12.6%, OR 0.34; 0.22–0.53) was not paralleled in inpatients with hematologic malignancies (32.3% vs. 34.8%, OR 1.12; 0.81–1.5). Among evaluable patients, 27.3% had post COVID-19 condition. These findings will help inform evidence-based preventive and therapeutic strategies for patients with hematologic malignancies and COVID-19 diagnosis.Depto. de MedicinaFac. de MedicinaTRUEFundación Madrileña de Hematología y HemoterapiaFundación Leucemia y LinfomaAsociación Madrileña de Hematología y Hemoterapiapu

The use of isothermal titration calorimetry to unravel chemotactic signalling mechanisms

Chemotaxis is based on the action of chemosensory pathways and is typically initiated by the recognition of chemoeffectors at chemoreceptor ligand-binding domains (LBD). Chemosensory signalling is highly complex; aspect that is not only reflected in the intricate interaction between many signalling proteins but also in the fact that bacteria frequently possess multiple chemosensory pathways and often a large number of chemoreceptors, which are mostly of unknown function. We review here the usefulness of isothermal titration calorimetry (ITC) to study this complexity. ITC is the gold standard for studying binding processes due to its precision and sensitivity, as well as its capability to determine simultaneously the association equilibrium constant, enthalpy change and stoichiometry of binding. There is now evidence that members of all major LBD families can be produced as individual recombinant proteins that maintain their ligand-binding properties. High-throughput screening of these proteins using thermal shift assays offer interesting initial information on chemoreceptor ligands, providing the basis for microcalorimetric analyses and microbiological experimentation. ITC has permitted the identification and characterization of many chemoreceptors with novel specificities. This ITC-based approach can also be used to identify signal molecules that stimulate members of other families of sensor proteins.This work was supported by FEDER funds and Fondo Social Europeo through grant BIO2016-76779-P from the Spanish Ministry for Economy and Competitiveness to TK

Pseudomonas aeruginosa as a Model To Study Chemosensory Pathway Signaling

Bacteria have evolved a variety of signal transduction mechanisms that generate different outputs in response to external stimuli. Chemosensory pathways are widespread in bacteria and are among the most complex signaling mechanisms, requiring the participation of at least six proteins. These pathways mediate flagellar chemotaxis, in addition to controlling alternative functions such as second messenger levels or twitching motility. The human pathogen Pseudomonas aeruginosa has four different chemosensory pathways that carry out different functions and are stimulated by signal binding to 26 chemoreceptors. Recent research employing a diverse range of experimental approaches has advanced enormously our knowledge on these four pathways, establishing P. aeruginosa as a primary model organism in this field. In the first part of this article, we review data on the function and physiological relevance of chemosensory pathways as well as their involvement in virulence, whereas the different transcriptional and posttranscriptional regulatory mechanisms that govern pathway function are summarized in the second part. The information presented will be of help to advance the understanding of pathway function in other organisms.This study was supported by FEDER funds and the Fondo Social Europeo through grants from the Spanish Ministry for Science and Innovation to M.A.M. (PID2019-103972GA-I00) and the Spanish Ministry of Economy and Competitiveness to J.A.G. (BIO2016-74875-P) and T.K. (BIO2016-76779-P)