HPC Cloud for Scientific and Business Applications: Taxonomy, Vision, and Research Challenges

High Performance Computing (HPC) clouds are becoming an alternative to on-premise clusters for executing scientific applications and business analytics services. Most research efforts in HPC cloud aim to understand the cost-benefit of moving resource-intensive applications from on-premise environments to public cloud platforms. Industry trends show hybrid environments are the natural path to get the best of the on-premise and cloud resources---steady (and sensitive) workloads can run on on-premise resources and peak demand can leverage remote resources in a pay-as-you-go manner. Nevertheless, there are plenty of questions to be answered in HPC cloud, which range from how to extract the best performance of an unknown underlying platform to what services are essential to make its usage easier. Moreover, the discussion on the right pricing and contractual models to fit small and large users is relevant for the sustainability of HPC clouds. This paper brings a survey and taxonomy of efforts in HPC cloud and a vision on what we believe is ahead of us, including a set of research challenges that, once tackled, can help advance businesses and scientific discoveries. This becomes particularly relevant due to the fast increasing wave of new HPC applications coming from big data and artificial intelligence.Comment: 29 pages, 5 figures, Published in ACM Computing Surveys (CSUR

Mitogen-activated protein kinase kinase 5 regulates proliferation and biosynthetic processes in procyclic forms of Trypanosoma brucei

The pathogenic protozoan T. brucei alternates into distinct developmental stages in the mammalian and insect hosts. The mitogen-activated protein kinase (MAPK) signaling pathways transduce extracellular stimuli into a range of cellular responses, which ultimately lead to the adaptation to the external environment. Here, we combined a loss of function approach with stable isotope labeling with amino acids in cell culture (SILAC)-based mass spectrometry (MS) to investigate the role of the mitogen-activated protein kinase kinase 5 (MKK5) in T. brucei. The silencing of MKK5 significantly decreased the proliferation of procyclic forms of T. brucei. To shed light on the molecular alterations associated with this phenotype, we measured the total proteome and phosphoproteome of cells silenced for MKK5. In the total proteome, we observed a general decrease in proteins related to ribosome and translation as well as down-regulation of several components of the fatty acids biosynthesis pathway. In addition, we observed alterations in the protein levels and phosphorylation of key metabolic enzymes, which point toward a suppression of the oxidative metabolism. Taken together, our findings show that the silencing of MKK5 alters cell growth, energy metabolism, protein and fatty acids biosynthesis in procyclic T. brucei

Electrically charged regular black holes in nonlinear electrodynamics: light rings, shadows, and gravitational lensing

Within nonlinear electrodynamics (NED), photons follow null geodesics of an effective geometry, which is different from the geometry of the spacetime itself. Over the last years, several works were dedicated to investigate the motion of photons in the effective geometry of NED-based magnetically charged regular black hole (RBH) solutions. However, there are few works considering electrically charged RBHs. We study the light rings, shadows, and gravitational lensing of the electrically charged RBH solution proposed by Irina Dymnikova (ID), which is a static and spherically symmetric spacetime with a NED source. We show that the shadow associated to the effective geometry can be almost 10% bigger that the one associated to the standard geometry. We also find that the ID solution may mimic the shadow properties of the Reissner-Nordstrom (RN) BH, for low- ¨ to-extreme values of the electric charge. Besides that, by using the backwards ray-tracing technique, we obtain that ID and RN BH solutions can have a very similar gravitational lensing, for some values of the correspondent electric charges. We also show that the motion of photons in the effective geometry can be interpreted as a non-geodesic curve submitted to a 4-force term, from the perspective of an observer in the standard geometry.publishe

Bond behavior between glulam and GFRP's by pullout tests

To evaluate the bond behavior between glulam and GFRP rods, applied according to the near-surface mounted strengthening technique, an experimental program composed of beam and direct pullout tests was carried. In this experimental program three main variables were analyzed: the GFRP type, the GFRP location into the groove, and the bond length. From the monitoring system it was registered the loaded and free end slips, and the pullout force. Based on these experimental results, and applying an analytical-numerical strategy, the local bond stress-slip relationship was calculated. In this work the tests are described, the obtained results are presented and discussed, and the applicability of the inverse analysis to obtain the local bond law is demonstrated.This work is supported by FEDER funds through the Operational Programme for Competitiveness Factors - COMPETE and National Funds through FCT – Portuguese Foundation for Science and Technology under the project PTDC/ECM/74337/2006. The authors also like to thank all the companies that have been involved supporting and contributing for the development of this study, mainly: INEGI, S&P Clever Reinforcement Ibérica Lda., Portilame, MAPEI and Rothoblaas

Oncostatin M promotes excitotoxicity by inhibiting glutamate uptake in astrocytes: implications in HIV-associated neurotoxicity

Background: Elevated levels of oncostatin M (OSM), an interleukin-6 cytokine family member, have been observed in HIV-1-associated neurocognitive disorders (HAND) and Alzheimer's disease. However, the function of OSM in these disease conditions is unclear. Since deficient glutamate uptake by astrocytes is instrumental in HAND-associated neurotoxicity, we hypothesized that OSM impairs glutamate uptake in astrocytes and thereby promotes neuronal excitotoxicity. Methods: Primary cultures of mouse cortical astrocytes, neurons, microglia, and BV2 cell line were used. The expression of glutamate transporters (GLAST/EAAT1 and GLT-1/EAAT2) was investigated using real-time PCR and Western blot, and their activity was assessed by measuring H-3-D-aspartate uptake. Neuronal toxicity was measured using the colorimetric MTT (3-(4,5-dimethylthiazol-2-yl-) 2,5-diphenyltetrazolium bromide) assay and immunocytochemistry. A chimeric HIV-1 that infects murine cells (EcoHIV/NL4-3-GFP virus (EcoHIV)) was used to investigate whether the virus induces OSM, OSM receptor (OSMR)-beta, glycoprotein 130 (gp130), GLT-1, GLAST (mRNA and protein), and OSM release (ELISA) in cultured BV2 cells, primary microglia, or astrocytes. Statistical analyses of the data were performed using one-way ANOVA (to allow multiple comparisons) and two-tailed Student's t test. Results: OSM treatment (10 ng/mL) time-dependently reduced GLAST and GLT-1 expression and inhibited 3H-D-aspartate uptake in cultured astrocytes in a concentration-dependent manner, an effect prevented by the Janus kinase (JAK)/signal transducers and activators of transcription (STAT) 3 inhibitor AG490. Down-regulation of astrocytic glutamate transport by OSM resulted in NMDA receptor-dependent excitotoxicity in cortical neurons. Infection with EcoHIV induced OSM gene expression and protein release in BV2 cells and microglia, but not in astrocytes. Conversely, EcoHIV caused a fivefold increase in OSMR-beta mRNA (but not gp130) and protein in astrocytes, but not in microglia, which did not express OSMR-beta protein. Finally, astrocytic expression of GLAST gene was unaffected by EcoHIV, whereas GLT-1 mRNA was increased by twofold. Conclusions: We provide first evidence that activation of JAK/STAT3 signaling by OSM inhibits glutamate uptake in astrocytes, which results in neuronal excitotoxicity. Our findings with EcoHIV suggest that targeting OSMR-beta signaling in astrocytes might alleviate HIV-1-associated excitotoxicity