16 research outputs found

    Structural and Functional adaptation of the lingual papillae of the Egyptian fruit bat (Rousettus aegyptiacus): Specific Adaptive feeding Strategies

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    The current investigation was directed to clarify the correlations between the feeding strategy and lingual structure of the Egyptian fruit bat captured from the Egyptian east desert. The current work depends on twelve adult Egyptian fruit bats that observed grossly and with the help of the stereo, light, and scanning electron microscope. There were three types of the lingual papillae; one mechanical filiform and two gustatory (fungiform and circumvallate). There were seven subtypes of filiform papillae were recognized on the seven lingual regions. There were scanty numbers of fungiform papillae distributed among the filiform papillae on the lingual tip and two lateral parts of apex and body while fungiform papillae completely absent in the median part. There were three circumvallate papillae. The central bulb of circumvallate papillae surrounded by one layer of two segmented circular annular bad. The lingual tip had cornflower-like and diamond-shaped filiform papillae. Histochemical results revealed that the lingual glands were a stronger AB-positive reaction and gave dark blue color, while the reaction for the PAS-stain was negative. Also, the glands exhibited a blue color as an indication of positive AB reactivity with combined AB-PAS staining

    Immunogenicity and efficacy of a bivalent vaccine against infectious bronchitis virus

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    Infectious bronchitis (IB) is a highly contagious viral disease and is responsible for considerable economic losses in the poultry industry, worldwide. To mitigate the IB-associated losses, multiple vaccines are being applied in the sector with variable successes and thus necessitating the development of a potent vaccine to protect against the IB in the poultry. In the present study, we investigated a bivalent live attenuated vaccine consisting of IB virus (IBV) strain H120 (GI-1 lineage) and D274 (GI-12 lineage) to evaluate its protection against heterologous variant of IBV (GI-23 lineage) in chicken. Protection efficacy was evaluated based on the serology, clinical signs, survival rates, tracheal and kidney histopathology and the viral shedding. Results demonstrated that administering live H120 and D274 (named here Classivar®) vaccine in one day-old and 14 days-old provided 100 % protection. We observed a significant increase in the mean antibody titers, reduced virus shedding, and ameliorated histopathology lesions compared to routinely used vaccination regimes. These results revealed that usage of different IBV vaccines combination can successfully ameliorate the clinical outcome and pathology in vaccinated chicks especially after booster vaccination regime using Classivar®. In conclusions, our data indicate that Classivar® vaccine is safe in chicks and may serve as an effective vaccine against the threat posed by commonly circulating IBV strains in the poultry industry
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