A Potential New Therapeutic Approach To Dmd: Pkc Theta Inhibition

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)161415Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2014/04782-6]Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) [302831/2013-4]Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq

Elaboração de preparações produzidas com plantas alimentícias não convencionais (panc’s) do cerrado: castanha de baru e ora-pro-nóbis

O Cerrado brasileiro é um dos biomas mais ricos do mundo abrigando diversas espécies, dentre elas, as Plantas Alimentícias Não Convencionais (PANC’S). As PANC’S são plantas que podem ser utilizadas na alimentação humana, porém a grande maioria não é ofertada para consumo alimentar devido a sua exclusão no planejamento produtivo do sistema alimentar. Apesar de apresentarem alto valor nutricional, não estão incluídas no hábito de consumo dos brasileiros. Assim este estudo experimental, com elaboração de receitas nutritivas, versáteis e viáveis com utilização de PANC’S do Cerrado: Castanha de Baru e Ora-Pro-Nóbis, e suas respectivas Fichas Técnicas de Preparação (FTP), tem por objetivo desenvolver seis preparações utilizando a Dipteryx alata Vog, popularmente conhecida como Baru, e a Pereskia Aculeata Miller, conhecida por Ora-Pro-Nóbis, a produção de Fichas Técnicas de Preparação, de modo a permitir uma avaliação sob o aspecto nutritivo das plantas e comparação das receitas elaboradas quanto às receitas tradicionais, composição nutricional de macronutrientes, fibras, ferro e cálcio das PANC’S e das preparações elaboradas, além de disseminar informações sobre as mesmas. Na discussão foi abordado, o valor nutricional, sistemas alimentares, falta de informação sobre as PANC’S, papel do nutricionista, preparações e comparação entre as preparações. A conclusão enfatiza o alto valor nutricional das plantas, a importância da inclusão das mesmas na alimentação da população abrangendo a biodiversidade alimentar, a relevância para a contribuição na prevenção de doenças de carências nutricionais específicas, como a anemia ferropriva, uma vez que apresentam em sua composição uma quantidade de ferro substancial, de sistemas alimentares sustentáveis e saudáveis, das além da necessidade de atuação do profissional da Nutrição em aprofundar-se no tema proposto que se relaciona com seu principal material de trabalho que é o alimento

Expression Of Calcium-buffering Proteins In Rat Intrinsic Laryngeal Muscles.

Intrinsic laryngeal muscles (ILM) are highly specialized muscles involved in phonation and airway protection, with unique properties that allow them to perform extremely rapid contractions and to escape from damage in muscle dystrophy. Due to that, they may differ from limb muscles in several physiological aspects. Because a better ability to handle intracellular calcium has been suggested to explain ILM unique properties, we hypothesized that the profile of the proteins that regulate calcium levels in ILM is different from that in a limb muscle. Calcium-related proteins were analyzed in the ILM, cricothyroid (CT), and tibialis anterior (TA) muscles from male Sprague-Dawley rats (8 weeks of age) using quantitative PCR and western blotting. Higher expression of key Ca(2+) regulatory proteins was detected in ILM compared to TA, such as the sarcoplasmic reticulum (SR) Ca(2+)-reuptake proteins (Sercas 1 and 2), the Na(+)/Ca(2+) exchanger, phospholamban, and the Ca(2+)-binding protein calsequestrin. Parvalbumin, calmodulin and the ATPase, Ca(2+)-transporting, and plasma membrane 1 were also expressed at higher levels in ILM compared to TA. The store-operated calcium entry channel molecule was decreased in ILM compared to the limb muscle and the voltage-dependent L-type and ryanodine receptor were expressed at similar levels in ILM and TA. These results show that ILM have a calcium regulation system profile suggestive of a better ability to handle calcium changes in comparison to limb muscles, and this may provide a mechanistic insight for their unique pathophysiological properties.

Scale of conflict in health care decision-making: an instrument adapted and validated for the Portuguese language

As diferentes opções disponibilizadas aos doentes no âmbito da saúde actualmente implicam em processos de tomada de decisão cada vez mais difíceis podendo desencadear conflito no decorrer da mesma. Foi nosso propósito, com este estudo, dispor de um instrumento que nos possibilite conhecer esta variável. Assim, propusemo-nos efectuar a adaptação transcultural e avaliar as propriedades psicométricas da versão portuguesa da Decisional Conflict Scale, que visa obter informações sobre a tomada de decisão e os factores que influenciam a escolha tomada. A amostra constituída por 521 estudantes de Enfermagem, teve como foco a tomada de decisão na síndrome gripal. Os resultados obtidos nos testes de confiabilidade revelam boa consistência interna para o total dos itens (α Cronbach=0,94). O estudo psicométrico permite-nos afirmar que a versão em Português da Decisional Conflict Scale, que denominamos Escala de Conflitos de Tomadas de Decisão em Saúde (ECTDS), é um instrumento fidedigno e válido.Las diferentes opciones disponibles para el paciente en el ámbito de la salud implican actualmente procesos de toma de decisiones cada vez más difíciles, pudiéndose desencadenar conflictos durante ellos. Objetivamos disponer de un instrumento que nos posibilitara conocer esta variable. Consecuentemente, nos propusimos efectuar la adaptación transcultural y evaluar las propiedades psicométricas de la versión portuguesa de la Decisional Conflict Scale, que apunta a obtener informaciones sobre toma de decisiones y factores que influyen en la elección tomada. La muestra, constituida por 521 estudiantes de Enfermería, se enfocó en la toma de decisiones en el síndrome gripal. Los resultados obtenidos en los tests de confiabilidad expresan buena consistencia interna para todos los ítems (α Cronbach=0,94). El estudio psicométrico nos permite afirmar que la versión en portugués de la Decisional Conflict Scale, que denominamos Escala de Conflictos de Toma de Decisiones en Salud (ECTDS), es un instrumento fidedigno y válido.The different options available to patients in the health environment now are implicated in increasingly difficult processes of decision-making, and may trigger conflict about them. This study had as its purpose, to develop an instrument that enabled us to know about this variable. Therefore, we decided to effect a transcultural adaptation and evaluation of psychometric properties of the Portuguese version of the Decisional Conflict Scale, which seeks information about decision-making and the factors that influence the choices made. The sample consisted of 521 nursing students, with a focus on decision-making regarding the flu syndrome. The results obtained on the reliability tests showed good internal consistency for all items (Cronbach α=0.94). The psychometric study allowed us to affirm that the Portuguese version of the Decisional Conflict Scale, which we call Scale of Conflicts in Decision-Making in Health (ECTDS), was a reliable and valid instrument

High-energy diet does not overcome the negative impact of conjugated linoleic acid on young broiler performance

The aim of this study was to evaluate the effect of conjugated linoleic acid (CLA) supplementation in diets with different energy levels in broiler performance. Birds were offered a starter (1-21 d), grower (22-35 d) and finisher (36-42 d) diets; wherein soybean oil was replaced by CLA. The study consisted of a 3 × 2 factorial arrangement with two CLA levels (0 and 1%) and three energy levels (3050, 3100 and 3150 ME kg-1 diet). During the grower and finisher periods, birds were fed diets with same energy level and CLA supplementation was maintained the same. Growth performance was assessed weekly, and carcass and cuts yield were assessed at 42d. Interaction effect of CLA by energy level was observed in broiler performance and carcass yield throughout the study (p > 0.05). During the overall period (1-42 d) broiler performance was not affected by CLA (p > 0.05).However, CLA supplementation (1%) decreased weight gain (p 0.05). The increase in dietary energy was not able to compensate the negative effect on growth performance of broilers supplemented with 1% CLA at the starter period

Attitudes and behaviors towards vaccination in Portuguese nursing students

Knowing the attitudes and behaviors of nursing students in relation to vaccination is important because they will soon be determinant for the health literacy of the population. Vaccination remains the most effective response in the fight against communicable diseases, including COVID-19 and influenza. The objective of this study is to analyze the attitudes and behaviors of Portuguese nursing students with regard to vaccination. A cross-sectional study was carried out, with data collection from nursing students at a university in Lisbon, Portugal. A sample of 216 nursing students was obtained, representing 67.1% of the students enrolled in this university. What stands out from the results of the questionnaire “Attitudes and Behaviors in Relation to Vaccination among Students of Health Sciences” is that for the majority of students the answers were positive; in addition, 84.7% had a completed vaccination schedule for COVID-19. Being a nursing student, being in the final years of the course and being a woman are the factors that most influence the positive attitude of the students. The results obtained are motivating, because these students will be the future health professionals most likely to integrate health promotion programs through vaccination.info:eu-repo/semantics/publishedVersio

Aplicação de métodos antropométricos no processo de enfermagem de pesquisa em enfermagem

Objectives: to identify anthropometric techniques and measurements related to nursing diagnoses and interventions, included in the NANDA International (NANDA-I) and Nursing Interventions Classification (NIC), respectively. Methods: descriptive study of the revision of the NANDA-I and NIC taxonomies by professors of Nursing of the Universidad Complutense de Madrid. Results: A total of 24 nursing diagnoses, 20 nursing interventions and 71 nursing activities were identified that require the use of anthropometric measurements. Conclusions: anthropometric measurements are necessary in the conduct of numerous nursing diagnoses and nursing interventions that are commonly used in healthcare practice. It would be useful to systematize the use of anthropometric techniques in the application of the nursing process and to standardize this training process.Objetivos: Identificar técnicas e medidas antropométricas relacionadas com diagnósticos e intervenções de enfermagem, incluídas na NANDA-Internacional (NANDA-I) e na Nursing Interventions Classification (NIC) respectivamente. Métodos: estudo descritivo. Revisão das taxonomias NANDA-I e NIC por Professores de Enfermagem da Universidade Complutense de Madrid. Resultados: foram identificados 24 diagnósticos de enfermagem, 20 intervenções de enfermagem e 71 atividades de enfermagem que exigem o uso de medidas antropométricas. Conclusões: as medidas antropométricas são necessárias na realização de diversos diagnósticos de enfermagem e intervenções de enfermagem comummente utilizadas na prática assistencial. Seria útil sistematizar o uso de técnicas antropométricas na aplicação do processo de enfermagem e padronizar o processo de formação.info:eu-repo/semantics/publishedVersio

Indicadores empíricos para a estruturação de um instrumento de coleta de dados em unidade cirúrgica

Este artigo teve como propósito descrever o desenvolvimento das primeiras fases da construção de um instrumento de coleta de dados para o cliente cirúrgico, com os objetivos de listar os indicadores empíricos encontrados na literatura a partir das necessidades humanas básicas de Horta, e identificar a frequência desses indicadores empíricos em clientes internos na clinica cirúrgica. Para listar os indicadores empíricos utilizou-se como referencial teórico a Teoria das Necessidades Humanas Básicas, de Horta, e a partir de uma revisão na literatura foi possível definir cada necessidade e identificar para cada uma delas os indicadores empíricos. Após essa busca, foi elaborado um instrumento contendo 367 indicadores empíricos, o qual foi utilizado com 61 clientes internos na clínica cirúrgica do HULW. Obteve-se como resultado 84 indicadores empíricos que compuseram a primeira versão do instrumento de coleta de dados para cliente adulto cirúrgico. Espera-se que o instrumento que será elaborado a partir desses resultados venha contribuir com a implantação da sistematização da assistência de enfermagem na clínica cirúrgica e uniformizar a forma de coleta de dados na referida clínica no momento da admissão dos clientes, voltada para a identificação das necessidades desses clientes

The attitude towards vaccination of health sciences students at a Spanish university improved over the first 18 months of the COVID-19 pandemic

It is important to know the attitudes of students of health sciences (SHSs) towards vaccination since they will be tomorrow’s health professionals. Vaccination is a powerful tool in the fight against COVID-19. The aim of the present, cross-sectional study was to examine how the COVID-19 pandemic has influenced the attitude of SHSs towards vaccination. Data were collected in the form of a questionnaire from all students of nursing, physiotherapy and chiropody matriculated at a Madrid University for the academic year 2019/2020 (i.e., before the start of the pandemic [Q1]), and from all those matriculated for the year 2021/22 (i.e., c18 months after the pandemic was declared [Q2]). A multivariate analysis was performed to identify the influence of sex, degree being studied, course year and the time of answering (Q1 or Q2), on the dimensions Beliefs, Behaviours and General Attitude. Overall, 1894 questionnaires were returned (934 [49.3%] for Q1, and 960 [50.7%] for Q2), of which 70.5% were completed by students of nursing, 14% by students of physiotherapy and 15.4% by those studying chiropody. In Q2, the results for all three dimensions were significantly better (p < 0.05). The most important influencing factors were being a student of nurs-ing, being in the final years of training (years 3 or 4), female gender and answering at the time of Q2. The results obtained are encouraging since student nurses (who showed Q1 and Q2 General Attitude scores of 3.34 and 3.47 (maximum possible 4), respectively [p < 0.05]) are the health professionals of tomorrow most likely to be involved in vaccination programmes.info:eu-repo/semantics/publishedVersio

Vaccine coverage against influenza and SARS-CoV-2 in health sciences students during COVID-19 pandemic in Spain

Vaccination against influenza and SARS-CoV-2 is recommended in health sciences students to reduce the risk of acquiring these diseases and transmitting them to patients. The aim of the study was to evaluate how the pandemic influenced the modification of influenza vaccination coverage during the 2019/2020 and 2021/2022 campaigns and to analyze the vaccination coverage against SARS-CoV-2 in health sciences students. A cross-sectional study was conducted among students of the Faculty of Nursing, Physiotherapy and Podiatry of the Complutense University of Madrid. A questionnaire was administered in two stages, the first, Q1, before the start of the pandemic, where we analyzed influenza coverage during the 2019/2020 campaign and a second, Q2, 18 months after the start of the pandemic where we analyzed influenza coverage during the 2021/2022 campaign and coverage against SARS-CoV-2. A total of 1894 students (58.78% of the total of those enrolled) participated. Flu vaccination coverage increased from 26.7% in Q1 to 35.0% in Q2 (p < 0.05), being higher in the age group older than 21 years, who studied nursing, were in their fourth year and lived with people at risk. Vaccination coverage against SARS-CoV-2 was very high (97.8%), especially in students vaccinated against influenza. Coverage of the influenza vaccine in health sciences students increased from 2019–2020 to 2021–2022, being higher in the age group older than 21 years, who studied nursing, were in their first and fourth year and lived with people at risk. Coverage of the SARS-CoV-2 vaccine in health sciences students was very high, especially in those vaccinated against influenza.info:eu-repo/semantics/publishedVersio