158 research outputs found

    Characteristics of Core Promoter Types with respect to Gene Structure and Expression in Arabidopsis thaliana

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    It is now well known that vertebrates use multiple types of core promoter to accomplish differentiated tasks in Pol II-dependent transcription. Several transcriptional characteristics are known to be associated with core types, including distribution patterns of transcription start sites (TSSs) and selection between tissue-specific and constitutive expression profiles. However, their relationship to gene structure is poorly understood. In this report, we carried a comparative analysis of three Arabidopsis core types, TATA, GA, and Coreless, with regard to gene structure. Our genome-wide investigation was based on the peak TSS positions in promoters that had been identified in a large-scale experimental analysis. This analysis revealed that the types of core promoter are related with the room for promoters that is measured as the distance from the TSS to the end of the upstream gene, the distance from the TSS to the start position of the coding sequence (CDS), and the number and species of the cis-regulatory elements. Of these, it was found that the distance from the TSS to the CDS has a tight, inverse correlation to the expression level, and thus the observed relationship to the core type appears to be indirect. However, promoter length and preference of cis-elements are thought to be a direct reflection of core type-specific transcriptional initiation mechanisms

    Effect of artificially-generated wind on removing guttation and dew droplets from rice leaf surface for controlling rice blast disease

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    The incidence and severity of leaf and panicle blasts were efficiently reduced in the wind-treated fields. Artificially-generated wind was performed on rice paddy field in order to measure the changes in temperature and humidity during and after the blowing process and to investigate the effects of sending wind on the formation and removal of guttation and dew droplets on rice leaves. A large electric fan was used to send wind with a depression angle of 30° at 4:00 am for 30-40 min. During the blowing process, the humidity percentage was 100% in both treated and control paddy fields, the temperature was 0.8°C higher in the treated than the control field and the dew weight was rapidly decreased from 21 to 8 g in 20 min in the treated field. After the blowing process, the temperature started to rise at 6:28 am (10 min earlier than the control block). It reached 29°C at 6:48 am (30 min earlier than the control block) and remained higher than the control block till 7:20 am (1-7°C higher). The humidity decreased to 95% at 6:30 am (20 min earlier than the control block) and remained 95% till 6:42 am, then rapidly decreased to reach 68% at 6:46 am, and finally reached 60% at 7:20 am. The humidity was 5-30% lower than that in the control block. Moreover, the dew weight increased to the initial level within 10 min and then decreased rapidly in both blocks starting from 6:20 am. Using wind speed of 4.3 m/s or 3.2 m/s for 60 s per rotation at 4:00 am, almost all the guttation droplets except those of less than 0.5 mm were removed from the surfaces of the 1st and 2nd uppermost leaves after two rotations (at velocity 4.3 m/s) or three rotations (at velocity 3.2 m/s) and the dew droplets were mostly removed after five rotations (at velocity 4.3 m/s) or nine rotations (at velocity 3.2 m/s). Sending wind at a speed of 3.2 m/s or faster removed guttation and dew droplets and suppressed the subsequent dew formation. Our data indicates that the removal of dew droplets on rice hills resulted in reduction of the disease development of Pyricularia oryzea.  Key words: Generated–wind, rice blast, microclimate, guttation, dew droplets
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