Analytical parameters of the microplate-based ORAC pyrogallol red assay

The analytical parameters of the microplatebased oxygen radicals absorbance capacity (ORAC) method using pyrogallol red (PGR) as probe (ORAC-PGR) are presented. In addition, the antioxidant capacity of commercial beverages, such as wines, fruit juices, and iced teas, is estimated. A good linearity of the area under the curve (AUC) versus Trolox concentration plots was obtained [AUC = (845 ± 110) + (23 ± 2) [Trolox, μM], R = 0.9961, n = 19]. QC experiments showed better precision and accuracy at the highest Trolox concentration (40 μM) with RSD and REC (recuperation) values of 1.7 and 101.0%, respectively. When red wine was used as sample, the method also showed good linearity [AUC = (787 ± 77) + (690 ± 60) [red wine, μL/mL]; R = 0.9926, n = 17], precision and accuracy with RSD values from 1.4 to 8.3%, and REC values that ranged from 89.7 to 103.8%. Additivity assays using solutions containing gallic acid and Trolox (or red wine) showed an additive protection of PGR given by the sample

Variable surface charge of humic acid-ferrihydrite composite: Influence of electrolytes on ciprofloxacin adsorption.

Antimicrobial compounds are found in a range of environments as pollutants. Here, we evaluated the influence of two common anions, NO3- and PO43-, on ciprofloxacin adsorption on humic acid/ferrihydrite composite (HADIG/Fh), synthetic ferrihydrite (Fh), and humic acid (HA-DIG) under controlled pH (7.0), ionic strength (0.1 M) and temperature (25 degrees C). All materials were characterized by isoelectric point (IEP), while the composite and the iron oxide were characterized by Miissbauer spectroscopy. Kinetic and isotherm adsorption studies were carried out using cyclic voltammetry (in KH2PO4) and square wave voltammetry (in KNO3). The application of kinetic models for both anions revealed Fh to fit to a pseudo second order model (R-2 = 0.941); while HA-DIG (R-2 = 0.950) and HA-DIG/Fh (R-2 = 0.993) were fitted to pseudo first order models. The adsorption results showed a high dependency electrolyte, especially in Fh, where different shape curves (H-type in KNO3 and Ctype in KH2PO4) and maximum experimental adsorbed amount C m were observed. This finding is supported by the distinct IEP values and change in sign of surface charge between the two ions. Finally, results suggest that HA-DIG could be potentially used in environmental remediation to remove antibiotics from natural matrices, though the risk of antibiotic transportation increased with depth in the soil profile.Comision Nacional de Investigacion Cientifica y Tecnologica (CONICYT) CONICYT FONDECYT 1130094 Puente 2018 P1811 Dicyt-USACH 021742PA Proyecto Fondo Fortalecimiento USA1799 Basal Funding for Scientific and Technological Centers of Excellence FB0807 CEDENNA CONICYT PIA/ANILLO ACM 17000

Application of a microplate-based orac-pyrogallol red assay for the estimation of antioxidant capacity: First action 2012.03

The method was approved by the Expert Review Panel on Strategic Foods Analytical Methods as First Action. See "Standards News," (2012) Inside Laboratory Management, March/April issue. The AOAC Stakeholder Panel on Strategic Foods Analytical Methods (SPSFAM) invites method users to provide feedback on the First Action methods. Feedback from method users will help verify that the methods are fit for purpose and are critical to gaining global recognition and acceptance of the methods. Comments can be sent directly to the corresponding author

Beneficial Interactive Effects Provided by an Arbuscular Mycorrhizal Fungi and Yeast on the Growth of <i>Oenothera picensis</i> Established on Cu Mine Tailings

Phytoremediation, an environmentally friendly and sustainable approach for addressing Cu-contaminated environments, remains underutilized in mine tailings. Arbuscular mycorrhizal fungi (AMF) play a vital role in reducing Cu levels in plants through various mechanisms, including glomalin stabilization, immobilization within fungal structures, and enhancing plant tolerance to oxidative stress. Yeasts also contribute to plant growth and metal tolerance by producing phytohormones, solubilizing phosphates, generating exopolysaccharides, and facilitating AMF colonization. This study aimed to assess the impact of AMF and yeast inoculation on the growth and antioxidant response of Oenothera picensis plants growing in Cu mine tailings amended with compost. Plants were either non-inoculated (NY) or inoculated with Meyerozyma guilliermondii (MG), Rhodotorula mucilaginosa (RM), or a combination of both (MIX). Plants were also inoculated with Claroideoglomus claroideum (CC), while others remained non-AMF inoculated (NM). The results indicated significantly higher shoot biomass in the MG-NM treatment, showing a 3.4-fold increase compared to the NY-NM treatment. The MG-CC treatment exhibited the most substantial increase in root biomass, reaching 5-fold that in the NY-NM treatment. Co-inoculation of AMF and yeast influenced antioxidant activity, particularly catalase and ascorbate peroxidase. Furthermore, AMF and yeast inoculation individually led to a 2-fold decrease in total phenols in the roots. Yeast inoculation notably reduced non-enzymatic antioxidant activity in the ABTS and CUPRAC assays. Both AMF and yeast inoculation promoted the production of photosynthetic pigments, further emphasizing their importance in phytoremediation programs for mine tailings