Identification of male-specific volatiles from Neartic and Neotropical stink bugs (Heteroptera: Pentatomidae).

Made available in DSpace on 2018-06-09T01:21:08Z (GMT). No. of bitstreams: 1 ID10018.pdf: 537102 bytes, checksum: 11c1f7944bfb17316f8117c170d8e47d (MD5) Previous issue date: 1997-04-28bitstream/item/178381/1/ID-10018.pd

Artifacts and Pheromone Blends from Nezara spp. and Other Stink Bugs (Heteroptera: Pentatomidae)

Isomeric com pounds reportedly released by mature males o f Nezara viridula as attractants for conspecific females are evidently artifacts formed by dimerization o f (£)-4-oxo-2-hexenal. Additional analyses o f the male-specific volatiles from Italian, Australian, Brazilian, and Japanese populations o f N. viridula verify that at least two distinctive pheromone strains exist, but an active synthetic pheromone has not yet been developed. Analyses o f volatiles from N. antennata and Acrosternum aseadum males are also reported showing that the native Japanese Nezara sp., and species in the sister genus Acrosternum, produce species-specific blends based on the same com pounds as N. viridula: (Z)-a-bisabolene (l-m ethyl-4-(l,5-im ethyl-(Z )-l,4-hexadienyl)-cyclohexene), and trans- and cis- 1,2-epoxides o f (Z)-a-bisabolene. The trans-/cis- I,2-epoxide ratio o f N. antennata is within the range found for most N. viridula populations, but the blend from Japanese N. viridula males deviates radically from those o f other conspecific populations

Wound contraction effects and antibacterial properties of Tualang honey on full-thickness burn wounds in rats in comparison to hydrofibre

<p>Abstract</p> <p>Background</p> <p>Full-thickness burn wounds require excision and skin grafting. Multiple surgical procedures are inevitable in managing moderate to severe full-thickness burns. Wound bed preparations prior to surgery are necessary in order to prevent wound infection and promote wound healing. Honey can be used to treat burn wounds. However, not all the honey is the same. This study aims to evaluate the wound contraction and antibacterial properties of locally-produced <it>Tualang </it>honey on managing full-thickness burn wounds <it>in vivo</it>.</p> <p>Methods</p> <p>Thirty-six female <it>Sprague Dawley </it>rats were randomly divided into three groups. Under anaesthesia, three full-thickness burn wounds were created on the dorsum of the rats. The full-thickness burn wounds were inoculated with a specific organism (10⁴), namely <it>Pseudomonas aeruginosa </it>(n = 12), <it>Klebsiella pneumoniae </it>(n = 12), or <it>Acinetobacter baumannii </it>(n = 12). The three burn wounds were dressed with <it>Tualang </it>honey, hydrofibre and hydrofibre silver respectively. Swab samples were obtained every 3 days (day 3, 6, 9, 12, 15, 18 and 21) for quantitative and semi-quantitative microbiological analyses. Clinical assessments, including observations concerning the appearance and wound size, were measured at the same time.</p> <p>Results</p> <p>There was a rapid 32.26% reduction in wound size by day 6 (<it>p </it>= 0.008) in the <it>Tualang </it>honey-treated wounds, and 49.27% by day 15 (<it>p </it>= 0.005). The wounds remained smaller by day 18 (<it>p </it>< 0.032). <it>Tualang </it>honey-treated rats demonstrated a reduction in bacterial growth in <it>Pseudomonas aeruginosa </it>inoculated wounds (<it>p </it>= 0.005). However, hydrofibre silver and hydrofibre-treated wounds are superior to honey-treated wounds with <it>Acinetobacter baumannii </it>(<it>p </it>= 0.035). There was no statistical significant of antibacterial property in <it>Klebsiella pneumonia </it>inoculated wounds.</p> <p>Conclusions</p> <p><it>Tualang </it>honey has better results with regards to its control of <it>Pseudomonas aeruginosa </it>and its wound contraction effects on full-thickness burn wound <it>in vivo</it>.</p

The Interdomain Linker of AAV-2 Rep68 Is an Integral Part of Its Oligomerization Domain: Role of a Conserved SF3 Helicase Residue in Oligomerization

The four Rep proteins of adeno-associated virus (AAV) orchestrate all aspects of its viral life cycle, including transcription regulation, DNA replication, virus assembly, and site-specific integration of the viral genome into the human chromosome 19. All Rep proteins share a central SF3 superfamily helicase domain. In other SF3 members this domain is sufficient to induce oligomerization. However, the helicase domain in AAV Rep proteins (i.e. Rep40/Rep52) as shown by its monomeric characteristic, is not able to mediate stable oligomerization. This observation led us to hypothesize the existence of an as yet undefined structural determinant that regulates Rep oligomerization. In this document, we described a detailed structural comparison between the helicase domains of AAV-2 Rep proteins and those of the other SF3 members. This analysis shows a major structural difference residing in the small oligomerization sub-domain (OD) of Rep helicase domain. In addition, secondary structure prediction of the linker connecting the helicase domain to the origin-binding domain (OBD) indicates the potential to form α-helices. We demonstrate that mutant Rep40 constructs containing different lengths of the linker are able to form dimers, and in the presence of ATP/ADP, larger oligomers. We further identified an aromatic linker residue (Y224) that is critical for oligomerization, establishing it as a conserved signature motif in SF3 helicases. Mutation of this residue critically affects oligomerization as well as completely abolishes the ability to produce infectious virus. Taken together, our data support a model where the linker residues preceding the helicase domain fold into an α-helix that becomes an integral part of the helicase domain and is critical for the oligomerization and function of Rep68/78 proteins through cooperative interaction with the OBD and helicase domains

Invading and expanding : range dynamics and ecological consequences of the Greater White-Toothed Shrew (Crocidura russula) invasion in Ireland

Establishing how invasive species impact upon pre-existing species is a fundamental question in ecology and conservation biology. The greater white-toothed shrew (Crocidura russula) is an invasive species in Ireland that was first recorded in 2007 and which, according to initial data, may be limiting the abundance/distribution of the pygmy shrew (Sorex minutus), previously Ireland’s only shrew species. Because of these concerns, we undertook an intensive live-trapping survey (and used other data from live-trapping, sightings and bird of prey pellets/nest inspections collected between 2006 and 2013) to model the distribution and expansion of C. russula in Ireland and its impacts on Ireland’s small mammal community. The main distribution range of C. russula was found to be approximately 7,600 km2 in 2013, with established outlier populations suggesting that the species is dispersing with human assistance within the island. The species is expanding rapidly for a small mammal, with a radial expansion rate of 5.5 km/yr overall (2008–2013), and independent estimates from live-trapping in 2012–2013 showing rates of 2.4–14.1 km/yr, 0.5–7.1 km/yr and 0–5.6 km/yr depending on the landscape features present. S. minutus is negatively associated with C. russula. S. minutus is completely absent at sites where C. russula is established and is only present at sites at the edge of and beyond the invasion range of C. russula. The speed of this invasion and the homogenous nature of the Irish landscape may mean that S. minutus has not had sufficient time to adapt to the sudden appearance of C. russula. This may mean the continued decline/disappearance of S. minutus as C. russula spreads throughout the island

Pheromone blends of predacious bugs (Hetoroptera: Pentatomidae: Podisus spp.).

Made available in DSpace on 2018-06-11T16:58:37Z (GMT). No. of bitstreams: 1 ID1001627.pdf: 789371 bytes, checksum: 33b219b732627ff42cf530bcce3a89b1 (MD5) Previous issue date: 1997-04-28bitstream/item/178414/1/ID-10016-2-7.pd

Pheromone blends of predacious bugs (Hetoroptera: Pentatomidae: Podisus spp.).

bitstream/item/178414/1/ID-10016-2-7.pd

Pheromone blends of predacious bugs (Hetoroptera: Pentatomidae: Podisus spp.).

Made available in DSpace on 2018-06-11T16:58:37Z (GMT). No. of bitstreams: 1 ID1001627.pdf: 789371 bytes, checksum: 33b219b732627ff42cf530bcce3a89b1 (MD5) Previous issue date: 1997-04-28bitstream/item/178414/1/ID-10016-2-7.pd