175 research outputs found
Molecular cloning, expression analysis and assignment of the porcine tumor necrosis factor superfamily member 10 gene (TNFSF10) to SSC13q34 -> q36 by fluorescence in situ hybridization and radiation hybrid mapping
We have cloned the complete coding region of the porcine TNFSF10 gene. The porcine TNFSF10 cDNA has an ORF of 870 nucleotides and shares 85 % identity with human TNFSF10, and 75% and 72% identity with rat and mouse Tnfsf10 coding sequences, respectively. The deduced porcine TNFSF10 protein consists of 289 amino acids with the calculated molecular mass of 33.5 kDa and a predicted pI of 8.15. The amino acid sequence similarities correspond to 86, 72 and 70% when compared with human, rat and mouse sequences, respectively. Nor-them blot analysis detected TNFSF10-specific transcripts (similar to 1.7 kb) in various organs of a 10-week-old pig, suggesting ubiquitous expression. Real-time RT-PCR studies of various organs from fetal (days 73 and 98) and postnatal stages (two weeks, eight months) demonstrated developmental and tissue-specific regulation of TNFSF10 mRNA abundance. The chromosomal location of the porcine TNFSF10 gene was determined by FISH of a specific BAC clone to metaphase chromosomes. This TNFSF10 BAC clone has been assigned to SSC13q34 -> q36. Additionally, the localization of the TNFSF10 gene was verified by RH mapping on the porcine IMpRH panel. Copyright (c) 2005S. KargerAG, Basel
Gammaherpesvirus Latency Accentuates EAE Pathogenesis: Relevance to Epstein-Barr Virus and Multiple Sclerosis
Epstein-Barr virus (EBV) has been identified as a putative environmental trigger of multiple sclerosis (MS), yet EBV's role in MS remains elusive. We utilized murine gamma herpesvirus 68 (ÎłHV-68), the murine homolog to EBV, to examine how infection by a virus like EBV could enhance CNS autoimmunity. Mice latently infected with ÎłHV-68 developed more severe EAE including heightened paralysis and mortality. Similar to MS, ÎłHV-68EAE mice developed lesions composed of CD4 and CD8 T cells, macrophages and loss of myelin in the brain and spinal cord. Further, T cells from the CNS of ÎłHV-68 EAE mice were primarily Th1, producing heightened levels of IFN-Îł and T-bet accompanied by IL-17 suppression, whereas a Th17 response was observed in uninfected EAE mice. Clearly, ÎłHV-68 latency polarizes the adaptive immune response, directs a heightened CNS pathology following EAE induction reminiscent of human MS and portrays a novel mechanism by which EBV likely influences MS and other autoimmune diseases
Limits on the high-energy gamma and neutrino fluxes from the SGR 1806-20 giant flare of December 27th, 2004 with the AMANDA-II detector
On December 27th 2004, a giant gamma flare from the Soft Gamma-ray Repeater
1806-20 saturated many satellite gamma-ray detectors. This event was by more
than two orders of magnitude the brightest cosmic transient ever observed. If
the gamma emission extends up to TeV energies with a hard power law energy
spectrum, photo-produced muons could be observed in surface and underground
arrays. Moreover, high-energy neutrinos could have been produced during the SGR
giant flare if there were substantial baryonic outflow from the magnetar. These
high-energy neutrinos would have also produced muons in an underground array.
AMANDA-II was used to search for downgoing muons indicative of high-energy
gammas and/or neutrinos. The data revealed no significant signal. The upper
limit on the gamma flux at 90% CL is dN/dE < 0.05 (0.5) TeV^-1 m^-2 s^-1 for
gamma=-1.47 (-2). Similarly, we set limits on the normalization constant of the
high-energy neutrino emission of 0.4 (6.1) TeV^-1 m^-2 s^-1 for gamma=-1.47
(-2).Comment: 14 pages, 3 figure
The search for transient astrophysical neutrino emission with IceCube-DeepCore
We present the results of a search for astrophysical sources of brief transient neutrino emission using IceCube and DeepCore data acquired between 2012 May 15 and 2013 April 30. While the search methods employed in this analysis are similar to those used in previous IceCube point source searches, the data set being examined consists of a sample of predominantly sub-TeV muon-neutrinos from the Northern Sky (-5 degrees < delta < 90 degrees) obtained through a novel event selection method. This search represents a first attempt by IceCube to identify astrophysical neutrino sources in this relatively unexplored energy range. The reconstructed direction and time of arrival of neutrino events are used to search for any significant self-correlation in the data set. The data revealed no significant source of transient neutrino emission. This result has been used to construct limits at timescales ranging from roughly 1 s to 10 days for generic soft-spectra transients. We also present limits on a specific model of neutrino emission from soft jets in core-collapse supernovae
First year performance of the IceCube neutrino telescope
The first sensors of the IceCube neutrino observatory were deployed at the South Pole during the austral summer of 2004-2005 and have been producing data since February 2005. One string of 60 sensors buried in the ice and a surface array of eight ice Cherenkov tanks took data until December 2005 when deployment of the next set of strings and tanks began. We have analyzed these data, demonstrating that the performance of the system meets or exceeds design requirements. Times are determined across the whole array to a relative precision of better than 3 ns, allowing reconstruction of muon tracks and light bursts in the ice, of air-showers in the surface array and of events seen in coincidence by surface and deep-ice detectors separated by up to 2.5 km
Exhausted Cytotoxic Control of Epstein-Barr Virus in Human Lupus
Systemic Lupus Erythematosus (SLE) pathology has long been associated with an increased Epstein-Barr Virus (EBV) seropositivity, viremia and cross-reactive serum antibodies specific for both virus and self. It has therefore been postulated that EBV triggers SLE immunopathology, although the mechanism remains elusive. Here, we investigate whether frequent peaks of EBV viral load in SLE patients are a consequence of dysfunctional anti-EBV CD8+ T cell responses. Both inactive and active SLE patients (nâ=â76 and 42, respectively), have significantly elevated EBV viral loads (Pâ=â0.003 and 0.002, respectively) compared to age- and sex-matched healthy controls (nâ=â29). Interestingly, less EBV-specific CD8+ T cells are able to secrete multiple cytokines (IFN-Îł, TNF-α, IL-2 and MIP-1ÎČ) in inactive and active SLE patients compared to controls (Pâ=â0.0003 and 0.0084, respectively). Moreover, EBV-specific CD8+ T cells are also less cytotoxic in SLE patients than in controls (CD107a expression: Pâ=â0.0009, Granzyme B release: Pâ=â0.0001). Importantly, cytomegalovirus (CMV)-specific responses were not found significantly altered in SLE patients. Furthermore, we demonstrate that EBV-specific CD8+ T cell impairment is a consequence of their Programmed Death 1 (PD-1) receptor up-regulation, as blocking this pathway reverses the dysfunctional phenotype. Finally, prospective monitoring of lupus patients revealed that disease flares precede EBV reactivation. In conclusion, EBV-specific CD8+ T cell responses in SLE patients are functionally impaired, but EBV reactivation appears to be an aggravating consequence rather than a cause of SLE immunopathology. We therefore propose that autoimmune B cell activation during flares drives frequent EBV reactivation, which contributes in a vicious circle to the perpetuation of immune activation in SLE patients
The ICECUBE prototype string in AMANDA
The Antarctic Muon And Neutrino Detector Array (Amanda) is a high-energy
neutrino telescope. It is a lattice of optical modules (OM) installed in the
clear ice below the South Pole Station. Each OM contains a photomultiplier tube
(PMT) that detects photons of Cherenkov light generated in the ice by muons and
electrons. IceCube is a cubic-kilometer-sized expansion of Amanda currently
being built at the South Pole. In IceCube the PMT signals are digitized already
in the optical modules and transmitted to the surface. A prototype string of 41
OMs equipped with this new all-digital technology was deployed in the Amanda
array in the year 2000. In this paper we describe the technology and
demonstrate that this string serves as a proof of concept for the IceCube
array. Our investigations show that the OM timing accuracy is 5 ns. Atmospheric
muons are detected in excellent agreement with expectations with respect to
both angular distribution and absolute rate
Limits to the muon flux from neutralino annihilations in the Sun with the AMANDA detector
A search for an excess of muon-neutrinos from neutralino annihilations in the
Sun has been performed with the AMANDA-II neutrino detector using data
collected in 143.7 days of live-time in 2001. No excess over the expected
atmospheric neutrino background has been observed. An upper limit at 90%
confidence level has been obtained on the annihilation rate of captured
neutralinos in the Sun, as well as the corresponding muon flux limit at the
Earth, both as functions of the neutralino mass in the range 100 GeV-5000 GeV.Comment: 13 pages, 3 figures. Submitted to Astropart. Phy
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