The Arches cluster revisited: I. Data presentation and stellar census

Context. Located within the central region of the Galaxy, the Arches cluster appears to be one of the youngest, densest and most massive stellar aggregates within the Milky Way. As such it has the potential to be a uniquely instructive laboratory for the study of star formation in extreme environments and the physics of very massive stars. Aims. To realise this possibility, the fundamental physical properties of both cluster and constituent stars need to be robustly determined; tasks we attempt here. Methods. In order to accomplish these goals we provide and analyse new multi-epoch near-IR spectroscopic data obtained with the VLT/SINFONI and photometry from the HST/WFC3. We are able to stack multiple epochs of spectroscopy for individual stars in order to obtain the deepest view of the cluster members ever obtained. Results. We present spectral classifications for 88 cluster members, all of which are WNLh or O stars: a factor of three increase over previous studies. We find no further examples of Wolf-Rayet stars within the cluster; importantly no H-free examples were identified. The smooth and continuous progression in spectral morphologies from O super-/hypergiants through to the WNLh cohort implies a direct evolutionary connection. We identify candidate giant and main sequence O stars spectroscopically for the first time. No products of binary evolution may be unambiguously identified despite the presence of massive binaries within the Arches. Conclusions. Notwithstanding difficulties imposed by the highly uncertain (differential) reddening to the Arches, we infer a main sequence/luminosity class V turn-off mass of ∼ 30 − 38M⊙ via the distribution of spectral types. Analysis of the eclipsing binary F2 suggests current masses of ∼ 80M⊙ and ∼ 60M⊙ for the WNLh and O hypergiant cohorts, respectively; we conclude that all classified stars have masses > 20M⊙. An age of ∼ 2.0 − 3.3Myr is suggested by the turn-off between ∼O4-5 V; constraints imposed by the supergiant population and the lack of H-free WRs are consistent with this estimate. While the absence of highly evolved WC stars strongly argues against the prior occurrence of SNe within the Arches, the derived age does accommodate such events for exceptionally massive stars. Further progress will require quantitative analysis of multiple individual cluster members in addition to further spectroscopic observations to better constrain the binary and main sequence populations; nevertheless it is abundantly clear that the Arches offers an unprecedented insight into the formation, evolution and death of the most massive stars Nature allows to form

Letter from F. H. Lohr to Langer, 1918

Letter dated April 29, 1918 from F. H. Lohr to William Langer about the night in Minot, ND where two force men [one of them undercover detective Kersy Gowin] were shot. Lohr informs Langer that the vice holes have all closed because they fear the police, and the drinking in Minot is worse than ever. Lohr followed around the chief of police trying to gain information on the shooters. Lohr tells Langer he is uncertain when he is able to come to Minot again. After only being married for a short period of time his money does not allow him to travel and stay for long periods of time. Lohr reassures Langer that he is still in his service. See also: 1154https://commons.und.edu/langer-papers/1159/thumbnail.jp

An updated stellar census of the Quintuplet cluster

Context. Found within the central molecular zone, the Quintuplet is one of the most massive young clusters in the Galaxy. As a consequence it offers the prospect of constraining stellar formation and evolution in extreme environments. However, current observations suggest that it comprises a remarkably diverse stellar population that is difficult to reconcile with an instantaneous formation event. Aims. To better understand the nature of the cluster our aim is to improve observational constraints on the constituent stars. Methods. In order to accomplish this goal we present Hubble Space Telescope/NICMOS+WFC3 photometry and Very Large Telescope/SINFONI+KMOS spectroscopy for ∼100 and 71 cluster members, respectively. Results. Spectroscopy of the cluster members reveals the Quintuplet to be far more homogeneous than previously expected. All supergiants are classified as either O7–8 Ia or O9–B0 Ia, with only one object of earlier (O5 I–III) spectral type. These stars form a smooth morphological sequence with a cohort of seven early-B hypergiants and six luminous blue variables and WN9-11h stars, which comprise the richest population of such stars of any stellar aggregate known. In parallel, we identify a smaller population of late-O hypergiants and spectroscopically similar WN8–9ha stars. No further H-free Wolf–Rayet (WR) stars are identified, leaving an unexpectedly extreme ratio of 13:1 for WC/WN stars. A subset of the O9–B0 supergiants are unexpectedly faint, suggesting they are both less massive and older than the greater cluster population. Finally, no main sequence objects were identifiable. Conclusions. Due to uncertainties over which extinction law to apply, it was not possible to quantitatively determine a cluster age via isochrone fitting. Nevertheless, we find an impressive coincidence between the properties of cluster members preceding the H-free WR phase and the evolutionary predictions for a single, non-rotating 60 M⊙ star; in turn this implies an age of ∼3.0–3.6 Myr for the Quintuplet. Neither the late O-hypergiants nor the low luminosity supergiants are predicted by such a path; we suggest that the former either result from rapid rotators or are the products of binary driven mass-stripping, while the latter may be interlopers. The H-free WRs must evolve from stars with an initial mass in excess of 60 M⊙ but it appears difficult to reconcile their observational properties with theoretical expectations. This is important since one would expect the most massive stars within the Quintuplet to be undergoing core-collapse/SNe at this time; since the WRs represent an evolutionary phase directly preceding this event,their physical properties are crucial to understanding both this process and the nature of the resultant relativistic remnant. As such, the Quintuplet provides unique observational constraints on the evolution and death of the most massive stars forming in the local, high metallicity Universe

New Cepheid variables in the young open clusters Berkeley 51 and Berkeley 55

As part of a wider investigation of evolved massive stars in Galactic open clusters, we have spectroscopically identified three candidate classical Cepheids in the little-studied clusters Berkeley 51, Berkeley 55 and NGC 6603. Using new multi-epoch photometry, we confirm that Be 51 #162 and Be 55 #107 are bona fide Cepheids, with pulsation periods of 9.83±0.01 d and 5.850±0.005 d respectively, while NGC 6603 star W2249 does not show significant photometric variability. Using the period-luminosity relationship for Cepheid variables, we determine a distance to Be 51 of 5.3 +1.0 −0.8 kpc and an age of 44 +9 −8 Myr, placing it in a sparsely-attested region of the Perseus arm. For Be 55, we find a distance of 2.2±0.3 kpc and age of 63 +12 −11 Myr, locating the cluster in the Local arm. Taken together with our recent discovery of a long-period Cepheid in the starburst cluster VdBH222, these represent an important increase in the number of young, massive Cepheids known in Galactic open clusters. We also consider new Gaia (data release 2) parallaxes and proper motions for members of Be 51 and Be 55; the uncertainties on the parallaxes do not allow us to refine our distance estimates to these clusters, but the well-constrained proper motion measurements furnish further confirmation of cluster membership. However, future final Gaia parallaxes for such objects should provide valuable independent distance measurements, improving the calibration of the period-luminosity relationship, with implications for the distance ladder out to cosmological scales

COMPARISON OF PROLIFERATING CELL NUCLEAR ANTIGEN (PCNA) STAINING AND BRDURD-LABELING INDEX UNDER DIFFERENT PROLIFERATIVE CONDITIONS IN-VITRO BY FLOW-CYTOMETRY

PC10 is a monoclonal antibody against proliferating cell nuclear antigen (PCNA). The staining pattern in immunochemistry depends on fixation and detergent extraction treatment. The aim of this study was to validate the flow cytometric PCNA assay against Bromodeoxyuridine-labelling index (BrdUrd-LI) under different proliferative conditions in vitro. Expression of PCNA in methanol fixed cells with, and without, prior detergent extraction with EDTA/Triton was compared to BrdUrd-labelling index in NIH-3T3 fibroblasts and human Caski tumour cells in exponential phase and under confluent conditions. Serum stimulation and serum starvation conditions were studied. The results for BrdUrd-LI and PCNA-index after extraction showed good correlation for 3T3 fibroblasts and for Caski cells, with some differences for serum withdrawn Caski cells. There was no correlation between the number of cells that were positive for PCNA without extraction and BrdUrd-LI. Spheroid cells with G(1)-DNA-content showed an almost synchronous recruitment and progression through the cell cycle after trypsination and replating. Tightly bound PCNA paralleled this synchronicity whereas total PCNA did not change significantly. The results demonstrate that immunochemical detection of non-extractable PCNA-index gives similar results as compared with BrdUrd-labelling index under different proliferative conditions in vitro for different monolayer cell lines, whereas without extraction PCNA does not correlate with BrdUrd-LI in these fast growing cell lines due to its long half-life. PCNA expression parallels the progression through the cell cycle in V79 spheroids, a primitive model of tumour growth

Robustness of sweeping-window arc therapy treatment sequences against intrafractional tumor motion

Purpose: Due to the potentially periodic collimator dynamic in volumetric modulated arc therapy (VMAT) dose deliveries with the sweeping-window arc therapy (SWAT) technique, additional manifestations of dosimetric deviations in the presence of intrafractional motion may occur. With a fast multileaf collimator (MLC), and a flattening filter free dose delivery, treatment times close to 60 s per fraction are clinical reality. For these treatment sequences, the human breathing period can be close to the collimator sweeping period. Compared to a random arrangement of the segments, this will cause a further degradation of the dose homogeneity. Methods: Fifty VMAT sequences of potentially moving target volumes were delivered on a two dimensional ionization chamber array. In order to detect interplay effects along all three coordinate axes, time resolved measurements were performed twice-with the detector aligned in vertical (V) or horizontal (H) orientation. All dose matrices were then moved within a simulation software by a time-dependent motion vector. The minimum relative equivalent uniform dose EUDr,m for all breathing starting phases was determined for each amplitude and period. Furthermore, an estimation of periods with minimum EUD was performed. Additionally, LINAC logfiles were recorded during plan delivery. The MLC, jaw, gantry angle, and monitor unit settings were continuously saved and used to calculate the correlation coefficient between the target motion and the dose weighed collimator motion component for each direction (CC, LR, AP) separately. Results: The resulting EUDr,m were EUDr,m(CCV) = (98.3 +/- 0.6)%, EUDr,m(CCH) = (98.6 +/- 0.5)%, EUDr,m(AP(V)) = (97.7 +/- 0.9)%, and EUDr,m(LRH) = (97.8 +/- 0.9)%. The overall minimum relative EUD observed for 360. arc midventilation treatments was 94.6%. The treatment plan with the shortest period and a minimum relative EUD of less than 97% was found at T = 6.1 s. For a partial 120 degrees arc, an EUDr,m = 92.0% was found. In all cases, a correlation coefficient above 0.5 corresponded to a minimum in EUD. Conclusions: With the advent of fast VMAT delivery techniques, nonrobust treatment sequences for human breathing patterns can be generated. These sequences are characterized by a large correlation coefficient between a target motion component and the corresponding collimator dynamic. By iteratively decreasing the maximum allowed dose rate, a low correlation coefficient and consequentially a robust treatment sequence are ensured. (C) 2015 Author(s). All article content, except where otherwise noted, is licensed under a Creative Commons Attribution 3.0 Unported License

The Arches cluster revisited: II. A massive eclipsing spectroscopic binary in the Arches cluster

We have carried out a spectroscopic variability survey of some of the most massive stars in the Arches cluster, using K-band observations obtained with SINFONI on the VLT. One target, F2, exhibits substantial changes in radial velocity; in combination with new KMOS and archival SINFONI spectra, its primary component is found to undergo radial velocity variation with a period of 10.483+/-0.002 d and an amplitude of ~350 km/s-1. A secondary radial velocity curve is also marginally detectable. We reanalyse archival NAOS-CONICA photometric survey data in combination with our radial velocity results to confirm this object as an eclipsing SB2 system, and the first binary identified in the Arches. We model it as consisting of an 82+/-12 M⊙ WN8-9h primary and a 60+/-8 M⊙ O5-6 Ia+ secondary, and as having a slightly eccentric orbit, implying an evolutionary stage prior to strong binary interaction. As one of four X-ray bright Arches sources previously proposed as colliding-wind massive binaries, it may be only the first of several binaries to be discovered in this cluster, presenting potential challenges to recent models for the Arches' age and composition. It also appears to be one of the most massive binaries detected to date; the primary's calculated initial mass of >~120 M⊙ would arguably make this the most massive binary known in the Galaxy

History of Middle Ear Involvement and Speech/Language Development in Late Talkers

Late-talking and normally speaking toddlers with and without histories of middle ear involvement were followed for 2 years to assess speech and expressive language outcomes. Results revealed no differences in expressive language outcome that could be attributed to history of middle ear involvement in either group. There did seem to be differences in outcome on measures of articulation that were associated with history of middle ear involvement. The implications of these findings for treatment of otitis media and for referral of late-talking toddlers for speech and language services are discussed