THE STRUCTURE OF EMPLOYMENT AND UNEMPLOYMENT IN A DECLINING RURAL COMMUNITY

Labor and Human Capital,

QuantiMus: A Machine Learning-Based Approach for High Precision Analysis of Skeletal Muscle Morphology.

Skeletal muscle injury provokes a regenerative response, characterized by the de novo generation of myofibers that are distinguished by central nucleation and re-expression of developmentally restricted genes. In addition to these characteristics, myofiber cross-sectional area (CSA) is widely used to evaluate muscle hypertrophic and regenerative responses. Here, we introduce QuantiMus, a free software program that uses machine learning algorithms to quantify muscle morphology and molecular features with high precision and quick processing-time. The ability of QuantiMus to define and measure myofibers was compared to manual measurement or other automated software programs. QuantiMus rapidly and accurately defined total myofibers and measured CSA with comparable performance but quantified the CSA of centrally-nucleated fibers (CNFs) with greater precision compared to other software. It additionally quantified the fluorescence intensity of individual myofibers of human and mouse muscle, which was used to assess the distribution of myofiber type, based on the myosin heavy chain isoform that was expressed. Furthermore, analysis of entire quadriceps cross-sections of healthy and mdx mice showed that dystrophic muscle had an increased frequency of Evans blue dye+ injured myofibers. QuantiMus also revealed that the proportion of centrally nucleated, regenerating myofibers that express embryonic myosin heavy chain (eMyHC) or neural cell adhesion molecule (NCAM) were increased in dystrophic mice. Our findings reveal that QuantiMus has several advantages over existing software. The unique self-learning capacity of the machine learning algorithms provides superior accuracy and the ability to rapidly interrogate the complete muscle section. These qualities increase rigor and reproducibility by avoiding methods that rely on the sampling of representative areas of a section. This is of particular importance for the analysis of dystrophic muscle given the "patchy" distribution of muscle pathology. QuantiMus is an open source tool, allowing customization to meet investigator-specific needs and provides novel analytical approaches for quantifying muscle morphology

Delayed resolution of acute inflammation during zymosan-induced arthritis in leptin-deficient mice

The severity of antigen-induced arthritis (AIA) is decreased in leptin-deficient ob/ob mice. However, joint inflammation in AIA depends on the immune response, which is impaired in ob/ob mice. In the present study we investigated the effects of leptin deficiency on zymosan-induced arthritis (ZIA), which is independent of adaptive immunity. Arthritis was induced by injection of zymosan into the knee joint. Joint swelling was similar after 6 and 24 hours in ob/ob and control mice. However, it remained elevated in ob/ob animals on day 3 whereas values normalized in controls. Histology revealed similar articular lesions in all animals on day 3, but on days 14 and 21 arthritis tended to be more severe in ob/ob mice. The acute phase response, reflected by circulating levels of IL-6 and serum amyloid A, was also more pronounced in ob/ob mice, although corticosterone was significantly elevated in these animals. Similar results were obtained in leptin receptor-deficient db/db mice. Thus, in contrast to AIA, ZIA is not impaired in leptin-deficient animals. On the contrary, resolution of acute inflammation appears to be delayed in the absence of leptin or leptin signalling, suggesting that chronic leptin deficiency interferes with adequate control of the inflammatory response in ZIA

Identifikasi dan Uji Penangkapan Radikal 2,2 Difenil-1-Pkrilhidrazil (DPPH) dari Ekstrak Kasar Pigmen Bakteri Simbion Rumput Laut

Abstrak Seaweed belongs to the group of natural antioxidants, these antioxidants are able to counteract free radicals. Secondary metabolites that are antioxidants include alkaloids, flavonoids, phenolic compounds, steroids, and terpenoids and carotenoids. This study aims to identify and test the radical scavenging of 2,2 diphenyl-1-pkrylhydrazyl (DPPH) from the crude extract of the macroalgae symbiont pigment Turbinaria conoides originating from the coastal waters of Hamadi Jayapura, Papua. The method used in this study is an experimental method by identifying pigments using Thin Chromatography (TLC), and for Antioxidant testing using the DPPH Method. The results of the study used the TLC plate Spot 1 on the yellow TLC plate, which had a Retardation frequency (Rf) value of 5/8 = 0.62 and was identified as a carotenoid. The pigment extract of the TC.11 symbiont bacterial pigment extract also has antioxidant potential which was tested by the DPPH method. See the IC50 value of TC.11 bacteria of 24315 ppm compared to the β-carotene marker of 4103 pp

Washington Legislation—1941

In undertaking to survey the work of the 1941 legislature the aim has not been to attain complete coverage. Space limitations and the time factor have dictated that only certain phases be considered and that brevity rather than complete analysis be the guide. In selecting topics for discussion the aim has been to give attention to those statutes which are likely to be of greatest concern to practicing lawyers. At the outset this meant that virtually all of the largest group of statutes, those dealing with the powers of governmental units, be eliminated. Of the remaining statutes all could not be discussed, or even mentioned, and while the selection has been arbitrary the attempt has been to focus upon those which appear to be of the most general interest. Special regard has been had for statutes affecting judicial procedure, to those of a regulatory character and to social legislation. Because of space and time limitations consideration of some material has necessarily been postponed until the next issue of the Review. Among the statutes which will be discussed in the later issue are those dealing with agriculture, banking, small loans, taxation, trusts, and workmen\u27s compensation. The survey has been a cooperative enterprise by the members of the law faculty of the University of Washington, with the valuable help of members of the student editorial board of the Review. In particular, Dean Falknor was assisted by Mr. Arthur Quigley. Mr. Robert Buck aided Professor O\u27Bryan. Professor Shattuck had the assistance of Mr. Herbert Droker, and Professor Sholley, of Mr. Snyder Jed King and Mr. Bayard Crutcher

Phase fluctuations in the ABC model

We analyze the fluctuations of the steady state profiles in the modulated phase of the ABC model. For a system of $L$ sites, the steady state profiles move on a microscopic time scale of order $L^3$. The variance of their displacement is computed in terms of the macroscopic steady state profiles by using fluctuating hydrodynamics and large deviations. Our analytical prediction for this variance is confirmed by the results of numerical simulations

Poor oral health including active caries in 187 UK professional male football players: clinical dental examination performed by dentists

The few studies that have assessed oral health in professional/elite football suggest poor oral health with minimal data on impact on performance. The aim of this research was to determine oral health in a representative sample of professional footballers in the UK and investigate possible determinants of oral health and self-reported impact on well-being, training and performance

Language, Truth, and Logic and the Anglophone reception of the Vienna Circle

A. J. Ayer’s Language, Truth, and Logic had been responsible for introducing the Vienna Circle’s ideas, developed within a Germanophone framework, to an Anglophone readership. Inevitably, this migration from one context to another resulted in the alteration of some of the concepts being transmitted. Such alterations have served to facilitate a number of false impressions of Logical Empiricism from which recent scholarship still tries to recover. In this paper, I will attempt to point to the ways in which LTL has helped to foster the various mistaken stereotypes about Logical Empiricism which were combined into the received view. I will begin by examining Ayer’s all too brief presentation of an Anglocentric lineage for his ideas. This lineage, as we shall see, simply omits the major 19th century Germanophone influences on the rise of analytic philosophy. The Germanophone ideas he presents are selectively introduced into an Anglophone context, and directed towards various concerns that arose within that context. I will focus on the differences between Carnap’s version of the overcoming of metaphysics, and Ayer’s reconfiguration into what he calls the elimination of metaphysics. Having discussed the above, I will very briefly outline the consequences that Ayer’s radicalisation of the Vienna Circle’s doctrines had on the subsequent Anglophone reception of Logical Empiricism