27 research outputs found
The structure of a GH149 ÎČâ(1 â 3) glucan phosphorylase reveals a new surface oligosaccharide binding site and additional domains that are absent in the disaccharideâspecific GH94 glucoseâÎČâ(1 â 3)âglucose (laminaribiose) phosphorylase
Glycoside phosphorylases (GPs) with specificity for ÎČ-(1 â 3)-gluco-oligosaccharides are potential candidate biocatalysts for oligosaccharide synthesis. GPs with this linkage specificity are found in two families thus farâglycoside hydrolase family 94 (GH94) and the recently discovered glycoside hydrolase family 149 (GH149). Previously, we reported a crystallographic study of a GH94 laminaribiose phosphorylase with specificity for disaccharides, providing insight into the enzyme's ability to recognize its' sugar substrate/product. In contrast to GH94, characterized GH149 enzymes were shown to have more flexible chain length specificity, with preference for substrate/product with higher degree of polymerization. In order to advance understanding of the specificity of GH149 enzymes, we herein solved X-ray crystallographic structures of GH149 enzyme Pro_7066 in the absence of substrate and in complex with laminarihexaose (G6). The overall domain organization of Pro_7066 is very similar to that of GH94 family enzymes. However, two additional domains flanking its catalytic domain were found only in the GH149 enzyme. Unexpectedly, the G6 complex structure revealed an oligosaccharide surface binding site remote from the catalytic site, which, we suggest, may be associated with substrate targeting. As such, this study reports the first structure of a GH149 phosphorylase enzyme acting on ÎČ-(1 â 3)-gluco-oligosaccharides and identifies structural elements that may be involved in defining the specificity of the GH149 enzymes
Druggable Allosteric Sites in ÎČ-Propeller Lectins
Carbohydrate-binding proteins (lectins) are auspicious targets in drug discovery to combat antimicrobial resistance; however, their non-carbohydrate drug-like inhibitors are still unavailable. Here, we present a druggable pocket in a ÎČ-propeller lectin BambL from Burkholderia ambifaria as a potential target for allosteric inhibitors. This site was identified employing 19Fâ
NMR fragment screening and a computational pocket prediction algorithm SiteMap. The structureâactivity relationship study revealed the most promising fragment with a dissociation constant of 0.3±0.1â
mM and a ligand efficiency of 0.3â
kcalâmolâ1âHAâ1 that affected the orthosteric site. This effect was substantiated by site-directed mutagenesis in the orthosteric and secondary pockets. Future drug-discovery campaigns that aim to develop small molecule inhibitors can benefit from allosteric sites in lectins as a new therapeutic approach against antibiotic-resistant pathogens
Unravelling the Specificity of Laminaribiose Phosphorylase from Paenibacillus sp. YMâ1 towards Donor Substrates Glucose/Mannose 1âPhosphate by Using Xâray Crystallography and Saturation Transfer Difference NMR Spectroscopy
Glycoside phosphorylases (GPs) carry out a reversible phosphorolysis of carbohydrates into oligosaccharide acceptors and the corresponding sugar 1âphosphates. The reversibility of the reaction enables the use of GPs as biocatalysts for carbohydrate synthesis. Glycosyl hydrolase family 94 (GH94), which only comprises GPs, is one of the most studied GP families that have been used as biocatalysts for carbohydrate synthesis, in academic research and in industrial production. Understanding the mechanism of GH94 enzymes is a crucial step towards enzyme engineering to improve and expand the applications of these enzymes in synthesis. In this work with a GH94 laminaribiose phosphorylase from Paenibacillus sp. YMâ1 (PsLBP), we have demonstrated an enzymatic synthesis of disaccharide 1 (ÎČâdâmannopyranosylâ(1â3)âdâglucopyranose) by using a natural acceptor glucose and noncognate donor substrate αâmannose 1âphosphate (Man1P). To investigate how the enzyme recognises different sugar 1âphosphates, the Xâray crystal structures of PsLBP in complex with Glc1P and Man1P have been solved, providing the first molecular detail of the recognition of a noncognate donor substrate by GPs, which revealed the importance of hydrogen bonding between the active site residues and hydroxy groups at C2, C4, and C6 of sugar 1âphosphates. Furthermore, we used saturation transfer difference NMR spectroscopy to support crystallographic studies on the sugar 1âphosphates, as well as to provide further insights into the PsLBP recognition of the acceptors and disaccharide products
Discovery of an RmlC/D fusion protein in the microalga <i>Prymnesium parvum</i> and its implications for NDP-ÎČ-L-rhamnose biosynthesis in microalgae
The 6-deoxy sugar L-rhamnose (L-Rha) is found widely in plant and microbial polysaccharides and natural products. The importance of this and related compounds in hostâpathogen interactions often means that L-Rha plays an essential role in many organisms. L-Rha is most commonly biosynthesized as the activated sugar nucleotide uridine 5-diphospho-L-rhamnose (UDP-L-Rha) or thymidine 5-diphospho-L-rhamnose (TDP-L-Rha). Enzymes involved in the biosynthesis of these sugar nucleotides have been studied in some detail in bacteria and plants, but the activated form of L-Rha and the corresponding biosynthetic enzymes have yet to be explored in algae. Here, using sugar-nucleotide profiling in two representative algae, Euglena gracilis and the toxin-producing microalga Prymnesium parvum, we show that levels of UDP- and TDP-activated L-Rha differ significantly between these two algal species. Using bioinformatics and biochemical methods, we identified and characterized a fusion of the RmlC and RmlD proteins, two bacteria-like enzymes involved in TDP-L-Rha biosynthesis, from P. parvum. Using this new sequence and also others, we explored L-Rha biosynthesis among algae, finding that although most algae contain sequences orthologous to plant-like L-Rha biosynthesis machineries, instances of the RmlC-RmlD fusion protein identified here exist across the Haptophyta and Gymnodiniaceae families of microalgae. On the basis of these findings, we propose potential routes for the evolution of nucleoside diphosphate -L-Rha (NDP-L-Rha) pathways among algae
A rapid synthesis of low-nanomolar divalent LecA inhibitors in four linear steps from d-galactose pentaacetate
Chronic infections with Pseudomonas aeruginosa are associated with
the formation of bacterial biofilms. The tetrameric P. aeruginosa lectin
LecA is a virulence factor and an anti-biofilm drug target. Increasing the
overall binding affinity by multivalent presentation of binding epitopes
can enhance the weak carbohydrateâligand interactions. Lownanomolar divalent LecA ligands/inhibitors with up to 260-fold
valency-normalized potency boost and excellent selectivity over
human galectin-1 were synthesized from D-galactose pentaacetate
and benzaldehyde-based linkers in four linear steps
Exploring the glycans of <i>Euglena gracilis</i>
Euglena gracilis is an alga of great biotechnological interest and extensive metabolic capacity, able to make high levels of bioactive compounds, such as polyunsaturated fatty acids, vitamins and ÎČ-glucan. Previous work has shown that Euglena expresses a wide range of carbohydrate-active enzymes, suggesting an unexpectedly high capacity for the synthesis of complex carbohydrates for a single-celled organism. Here, we present an analysis of some of the carbohydrates synthesised by Euglena gracilis. Analysis of the sugar nucleotide pool showed that there are the substrates necessary for synthesis of complex polysaccharides, including the unusual sugar galactofuranose. Lectin- and antibody-based profiling of whole cells and extracted carbohydrates revealed a complex galactan, xylan and aminosugar based surface. Protein N-glycan profiling, however, indicated that just simple high mannose-type glycans are present and that they are partially modified with putative aminoethylphosphonate moieties. Together, these data indicate that Euglena possesses a complex glycan surface, unrelated to plant cell walls, while its protein glycosylation is simple. Taken together, these findings suggest that Euglena gracilis may lend itself to the production of pharmaceutical glycoproteins
Targeting the Central Pocket of the Pseudomonas aeruginosa Lectin LecA
Pseudomonas aeruginosa is an opportunistic ESKAPE pathogen
that produces two lectins, LecA and LecB, as part of its large
arsenal of virulence factors. Both carbohydrate-binding proteins
are central to the initial and later persistent infection processes,
i. e. bacterial adhesion and biofilm formation. The biofilm matrix
is a major resistance determinant and protects the bacteria
against external threats such as the host immune system or
antibiotic treatment. Therefore, the development of drugs
against the P. aeruginosa biofilm is of particular interest to
restore efficacy of antimicrobials. Carbohydrate-based inhibitors
for LecA and LecB were previously shown to efficiently reduce
biofilm formations. Here, we report a new approach for
inhibiting LecA with synthetic molecules bridging the established carbohydrate-binding site and a central cavity located
between two LecA protomers of the lectin tetramer. Inspired by
in silico design, we synthesized various galactosidic LecA
inhibitors with aromatic moieties targeting this central pocket.
These compounds reached low micromolar affinities, validated
in different biophysical assays. Finally, X-ray diffraction analysis
revealed the interactions of this compound class with LecA. This
new mode of action paves the way to a novel route towards
inhibition of P. aeruginosa biofilms
Targeting the Central Pocket of the Pseudomonas aeruginosa Lectin LecA
Pseudomonas aeruginosa is an opportunistic ESKAPE pathogen that produces two lectins, LecA and LecB, as part of its large arsenal of virulence factors. Both carbohydrate-binding proteins are central to the initial and later persistent infection processes, i.âe. bacterial adhesion and biofilm formation. The biofilm matrix is a major resistance determinant and protects the bacteria against external threats such as the host immune system or antibiotic treatment. Therefore, the development of drugs against the P. aeruginosa biofilm is of particular interest to restore efficacy of antimicrobials. Carbohydrate-based inhibitors for LecA and LecB were previously shown to efficiently reduce biofilm formations. Here, we report a new approach for inhibiting LecA with synthetic molecules bridging the established carbohydrate-binding site and a central cavity located between two LecA protomers of the lectin tetramer. Inspired by in silico design, we synthesized various galactosidic LecA inhibitors with aromatic moieties targeting this central pocket. These compounds reached low micromolar affinities, validated in different biophysical assays. Finally, X-ray diffraction analysis revealed the interactions of this compound class with LecA. This new mode of action paves the way to a novel route towards inhibition of P. aeruginosa biofilms
Targeting undruggable carbohydrate recognition sites through focused fragment library design
Carbohydrate-protein interactions are key for cell-cell and host-pathogen recognition and
thus, emerged as viable therapeutic targets. However, their hydrophilic nature poses major
limitations to the conventional development of drug-like inhibitors. To address this shortcoming, four fragment libraries were screened to identify metal-binding pharmacophores
(MBPs) as novel scaffolds for inhibition of Ca2+-dependent carbohydrate-protein interactions. Here, we show the effect of MBPs on the clinically relevant lectins DC-SIGN, Langerin,
LecA and LecB. Detailed structural and biochemical investigations revealed the specificity of
MBPs for different Ca2+-dependent lectins. Exploring the structure-activity relationships of
several fragments uncovered the functional groups in the MBPs suitable for modification to
further improve lectin binding and selectivity. Selected inhibitors bound efficiently to DCSIGN-expressing cells. Altogether, the discovery of MBPs as a promising class of Ca2+-
dependent lectin inhibitors creates a foundation for fragment-based ligand design for future
drug discovery campaigns
Insights into toxic prymnesium parvum blooms:The role of sugars and algal viruses
Prymnesium parvum is a toxin-producing microalga that causes harmful algal blooms globally, which often result in large-scale fish kills that have severe ecological and economic implications. Although many toxins have previously been isolated from P. parvum, ambiguity still surrounds the responsible ichthyotoxins in P. parvum blooms and the biotic and abiotic factors that promote bloom toxicity. A major fish kill attributed to P. parvum occurred in Spring 2015 on the Norfolk Broads, a low-lying set of channels and lakes (Broads) found on the East of England. Here, we discuss how water samples taken during this bloom have led to diverse scientific advances ranging from toxin analysis to discovery of a new lytic virus of P. parvum, P. parvum DNA virus (PpDNAV-BW1). Taking recent literature into account, we propose key roles for sialic acids in this type of viral infection. Finally, we discuss recent practical detection and management strategies for controlling these devastating blooms