Pattern formation of microtubules and motors: inelastic interaction of polar rods

We derive a model describing spatio-temporal organization of an array of microtubules interacting via molecular motors. Starting from a stochastic model of inelastic polar rods with a generic anisotropic interaction kernel we obtain a set of equations for the local rods concentration and orientation. At large enough mean density of rods and concentration of motors, the model describes orientational instability. We demonstrate that the orientational instability leads to the formation of vortices and (for large density and/or kernel anisotropy) asters seen in recent experiments.Comment: 4 pages, 5 figures, to appear in Phys. Rev. E, Rapid Communication

Curvature suppresses the Rayleigh-Taylor instability

The dynamics of a thin liquid film on the underside of a curved cylindrical substrate is studied. The evolution of the liquid layer is investigated as the film thickness and the radius of curvature of the substrate are varied. A dimensionless parameter (a modified Bond number) that incorporates both geometric parameters, gravity, and surface tension is identified, and allows the observations to be classified according to three different flow regimes: stable films, films with transient growth of perturbations followed by decay, and unstable films. Experiments and theory confirm that, below a critical value of the Bond number, curvature of the substrate suppresses the Rayleigh-Taylor instability

Opening of DNA double strands by helicases. Active versus passive opening

Helicase opening of double-stranded nucleic acids may be "active" (the helicase directly destabilizes the dsNA to promote opening) or "passive" (the helicase binds ssNA available due to a thermal fluctuation which opens part of the dsNA). We describe helicase opening of dsNA, based on helicases which bind single NA strands and move towards the double-stranded region, using a discrete ``hopping'' model. The interaction between the helicase and the junction where the double strand opens is characterized by an interaction potential. The form of the potential determines whether the opening is active or passive. We calculate the rate of passive opening for the helicase PcrA, and show that the rate increases when the opening is active. Finally, we examine how to choose the interaction potential to optimize the rate of strand separation. One important result is our finding that active opening can increase the unwinding rate by 7 fold compared to passive opening.Comment: 13 pages, 3 figure

Hyperlipasemia in dogs with acute kidney injury treated with and without hemodialysis.

BACKGROUND Hyperlipasemia has been reported in dogs with acute kidney injury (AKI) but associations with AKI severity, hemodialysis (HD) treatment, and outcome have not been extensively evaluated. OBJECTIVES Investigate the prevalence and clinical relevance of hyperlipasemia in dogs with AKI, treated with and without HD. ANIMALS Client-owned dogs (n = 125) with AKI. METHODS Retrospective data extraction from medical records, including signalment, cause of AKI, duration of hospitalization, survival, plasma creatinine concentration, and 1,2-o-dilauryl-rac-glycero-3-glutaric acid-(6'-methyresorufin) ester (DGGR) lipase activity at admission and throughout hospitalization. RESULTS A DGGR-lipase activity >3× the upper reference limit (URL) was found in 28.8% and 55.4% of dogs at admission and during hospitalization, respectively, but only 8.8% and 14.9% of dogs, respectively, were diagnosed with acute pancreatitis. Hyperlipasemia >10 × URL was observed in 32.7% of dogs during hospitalization. The DGGR-lipase activity was higher in dogs with International Renal Interest Society (IRIS) Grades 4-5 than Grades 1-3, but correlation between DGGR-lipase activity and creatinine concentration was poor (rs  = .22; 95% confidence intervals [CI], 0.04-0.38). Treatment with HD was not associated with DGGR-lipase activity independent of IRIS grade. Survival to discharge and 30 days after admission was 65.6% and 59.6%, respectively. High IRIS grades (P = .03) and high DGGR-lipase activity at admission (P = .02) and during hospitalization (P = .003) were associated with nonsurvival. CONCLUSIONS AND CLINICAL IMPORTANCE Hyperlipasemia is frequent and often marked in dogs with AKI despite only a minority being diagnosed with pancreatitis. Hyperlipasemia is associated with AKI severity but not independently with HD treatment. High IRIS grade and hyperlipasemia were associated with nonsurvival

CCR4-bearing T cells participate in autoimmune diabetes.

Chemokine receptor expression is exquisitely regulated on T cell subsets during the course of their migration to inflammatory sites. In the present study we demonstrate that CCR4 expression marks a pathogenic population of autoimmune T cells. CCR4 was found exclusively on memory CD4(+) T cells during the progression of disease in NOD mice. Cells expressing the CCR4 ligand TARC (thymus- and activation-regulated chemokine) were detected within infiltrated islets from prediabetic mice. Interestingly, neutralization of macrophage-derived chemokine (MDC) with Ab caused a significant reduction of CCR4-positive T cells within the pancreatic infiltrates and inhibited the development of insulitis and diabetes. Furthermore, enhanced recruitment of CCR4-bearing cells in NOD mice resulting from transgenic expression of MDC resulted in acceleration of clinical disease. Cumulatively, the results demonstrate that CCR4-bearing T cells participate in the development of such tissue-driven autoimmune reactions

Hydrodynamic attraction of swimming microorganisms by surfaces

Cells swimming in confined environments are attracted by surfaces. We measure the steady-state distribution of smooth-swimming bacteria (Escherichia coli) between two glass plates. In agreement with earlier studies, we find a strong increase of the cell concentration at the boundaries. We demonstrate theoretically that hydrodynamic interactions of the swimming cells with solid surfaces lead to their re-orientation in the direction parallel to the surfaces, as well as their attraction by the closest wall. A model is derived for the steady-state distribution of swimming cells, which compares favorably with our measurements. We exploit our data to estimate the flagellar propulsive force in swimming E. coli

Three Dimensional Structure and Energy Balance of a Coronal Mass Ejection

The Ultraviolet Coronagraph Spectrometer (UVCS) observed Doppler shifted material of a partial Halo Coronal Mass Ejection (CME) on December 13 2001. The observed ratio of [O V]/O V] is a reliable density diagnostic important for assessing the state of the plasma. Earlier UVCS observations of CMEs found evidence that the ejected plasma is heated long after the eruption. We have investigated the heating rates, which represent a significant fraction of the CME energy budget. The parameterized heating and radiative and adiabatic cooling have been used to evaluate the temperature evolution of the CME material with a time dependent ionization state model. The functional form of a flux rope model for interplanetary magnetic clouds was also used to parameterize the heating. We find that continuous heating is required to match the UVCS observations. To match the O VI-bright knots, a higher heating rate is required such that the heating energy is greater than the kinetic energy. The temperatures for the knots bright in Ly$\alpha$ and C III emission indicate that smaller heating rates are required for those regions. In the context of the flux rope model, about 75% of the magnetic energy must go into heat in order to match the O VI observations. We derive tighter constraints on the heating than earlier analyses, and we show that thermal conduction with the Spitzer conductivity is not sufficient to account for the heating at large heights.Comment: 40 pages, 16 figures, accepted for publication in ApJ For associated mpeg file, please see https://www.cora.nwra.com/~jylee/mpg/f5.mp

24^{24}Mg(pp, α\alpha)21^{21}Na reaction study for spectroscopy of 21^{21}Na

The $^{24}$Mg($p$, $\alpha$)$^{21}$Na reaction was measured at the Holifield Radioactive Ion Beam Facility at Oak Ridge National Laboratory in order to better constrain spins and parities of energy levels in $^{21}$Na for the astrophysically important $^{17}$F($\alpha, p$)$^{20}$Ne reaction rate calculation. 31 MeV proton beams from the 25-MV tandem accelerator and enriched $^{24}$Mg solid targets were used. Recoiling $^{4}$He particles from the $^{24}$Mg($p$, $\alpha$)$^{21}$Na reaction were detected by a highly segmented silicon detector array which measured the yields of $^{4}$He particles over a range of angles simultaneously. A new level at 6661 $\pm$ 5 keV was observed in the present work. The extracted angular distributions for the first four levels of $^{21}$Na and Distorted Wave Born Approximation (DWBA) calculations were compared to verify and extract angular momentum transfer.Comment: 11 pages, 6 figures, proceedings of the 18th International Conference on Accelerators and Beam Utilization (ICABU2014

Observations of a rotating macrospicule associated with an X-ray jet

We attempt to understand the driving mechanism of a macrospicule and its relationship with a coronal jet. We study the dynamics of a macrospicule and an associated coronal jet captured by multi-spacecraft observations. Doppler velocities both in the macrospicule and the coronal jet are determined by EIS and SUMER spectra. Their temporal evolution is studied using X-ray and He II 304 images. A blueshift of -120+/-15 km/s is detected on one side of the macrospicule, while a redshift of 50+/-6 km/s is found at the base of the other side. The inclination angle of the macrospicule inferred from a stereoscopic analysis with STEREO suggests that the measured Doppler velocities can be attributed to a rotating motion of the macrospicule rather than a radial flow or an expansion. The macrospicule is driven by the unfolding motion of a twisted magnetic flux rope, while the associated X-ray jet is a radial outflow.Comment: 4 pages, 3 figures, accepted for publication in A&