Impact of electromagnetic field on the pathogenicity of selected entomopathogenic bacteria (Proteus sp. and Bacillus sp.) on tick (Rhipicephalus decoloratus)

The effect of exposing entomopathogenic bacteria isolated from tick (Rhipicephalus decoloratus) to variable intensities of electromagnetic field for different periods of time was examined on their pathogenicity on tick. Some bacterial isolates from the macerate of tick cadavers were used in the infection of healthy engorged ticks. Two of the bacteria tested namely Proteus mirabilis and Bacillus cereus were able to induce morbid conditions in the ticks both of which were re-isolated from the resulting tick cadavers. Re-infection of different concentrations of both entomopathogenic bacteria of ticks were carried out on the tested ticks to determine the minimum lethal concentration required for pathogenicity. The result reveals that P. mirabilis and B. cereus were able to cause disease at concentrations of 106 and 107 cfu/ml, respectively. These bacteria were then exposed to electromagnetic field with different intensities for different periods of time after which they were reinfected into healthy insects. Conclusively, the study reveals some signs of stimulation in the entomopathogenic potency of B. cereus after exposure to electromagnetic field (EMF) when compared to the control treatments while a significant reduction in the  entomopathogenic potency of P. mirabilis was recorded.Keywords: Entomopathogens, electromagnetic field, Proteus mirabilis, Bacillus cereus.African Journal of Biotechnology Vol. 12(29), pp. 4683-469

Socioeconomic, Demographic and Policy Comparisons of Living and Deceased Kidney Transplantation Rates Across 53 Countries.

There are more than 1.7 million sufferers of end stage kidney disease (ESKD) worldwide and for many a donated kidney provides the only chance of regaining independence from dialysis. Unfortunately, the demand for kidneys for transplantation far exceeds the available supply. It is important, therefore, that we understand the factors that may influence kidney donation rates. While certain socio-demographic factors have been linked to kidney donation rates, few studies have examined the influence of multiple socio-demographic factors on rates of both living and deceased kidney transplantation (KT) and none have examined their comparative effect in large numbers of culturally and socio-politically diverse countries. In this study, we performed univariate and multivariate analyses of the influence of 15 socio-economic factors on both the living donor (LD) and the deceased donor (DD) kidney transplantation rates (KTR) in 54 countries. Our analyses demonstrated that factors such as UN HDI (United Nations Human Development Index), religion, education, age, healthcare expenditure, presumed consent legislation and existence of a nationally managed organ donation program were associated with higher deceased KTR. In contrast, the only factors associated with living KTR were a highly significant negative association with presumed consent and variable associations with different religions. We suggest that by identifying factors that affect kidney transplantation rates these can be used to develop programs for enhancing donor rates in individual countries where those rates are below the leading countries. Keywords Organ Donation, Kidney Transplantation; Living Donor Transplantation, Deceased Donor Kidneys, Socioeconomics; Public Policy; Demographics; Presumed Consent Lay summary Using data from 53 countries, this study shows that Human Development Index, religion, Gross Domestic Product (GDP), education, age, healthcare expenditure, presumed consent legislation and existence of a nationally managed organ donation programme were associated with higher deceased donation rates. For living transplants, a negative association was noted with presumed consent and variable associations with different religions. These factors could be targeted in countries with low donation rates

Seismic Azimuthal Anisotropy Beneath a Fast Moving Ancient Continent: Constraints from Shear Wave Splitting Analysis in Australia

Seismic Azimuthal Anisotropy Beneath Australia is Investigated using Splitting of the Teleseismic PKS, SKKS, and SKS Phases to Delineate Asthenospheric Flow and Lithospheric Deformation Beneath One of the Oldest and Fast-Moving Continents on Earth. in Total 511 Pairs of High-Quality Splitting Parameters Were Observed at 116 Seismic Stations. Unlike Other Stable Continental Areas in Africa, East Asia, and North America, Where Spatially Consistent Splitting Parameters Dominate, the Fast Orientations and Splitting Times Observed in Australia Show a Complex Pattern, with a Slightly Smaller Than Normal Average Splitting Time of 0.85 ± 0.33 S. on the North Australian Craton, the Fast Orientations Are Mostly N-S, which is Parallel to the Absolute Plate Motion (APM) Direction in the Hotspot Frame. Those Observed in the South Australian Craton Are Mostly NE-SW and E-W, Which Are Perpendicular to the Maximum Lithospheric Horizontal Shortening Direction. in East Australia, the Observed Azimuthal Anisotropy Can Be Attributed to Either APM Induced Simple Shear or Lithospheric Fabric Parallel to the Strike of the Orogenic Belts. the Observed Spatial Variations of the Seismic Azimuthal Anisotropy, When Combined with Results from Depth Estimation Utilizing the Spatial Coherency of the Splitting Parameters and Seismic Tomography Studies, suggest that the Azimuthal Anisotropy in Australia Can Mostly Be Related to Simple Shear in the Rheologically Transition Layer between the Lithosphere and Asthenosphere. Non-APM Parallel Anisotropy is Attributable to Modulations of the Mantle Flow System by Undulations of the Bottom of the Lithosphere, with a Spatially Variable Degree of Contribution from Lithospheric Fabric

Helping someone with problem drug use: a delphi consensus study of consumers, carers, and clinicians

<p>Abstract</p> <p>Background</p> <p>Problem use of illicit drugs (i.e. drug abuse or dependence) is associated with considerable health and social harms, highlighting the need for early intervention and engagement with health services. Family members, friends and colleagues play an important role in supporting and assisting individuals with problem drug use to seek professional help, however there are conflicting views about how and when such support should be offered. This paper reports on the development of mental health first aid guidelines for problem drug use in adults, to help inform community members on how to assist someone developing problem drug use or experiencing a drug-related crisis.</p> <p>Methods</p> <p>A systematic review of the scientific and lay literature was conducted to develop a 228-item survey containing potential first-aid strategies to help someone developing a drug problem or experiencing a drug-related crisis. Three panels of experts (29 consumers, 31 carers and 27 clinicians) were recruited from Australia, Canada, New Zealand, the United Kingdom, and the United States. Panel members independently rated the items over three rounds, with strategies reaching consensus on importance written into the guidelines.</p> <p>Results</p> <p>The overall response rate across three rounds was 80% (86% consumers, 81% carers, 74% clinicians). 140 first aid strategies were endorsed as essential or important by 80% or more of panel members. The endorsed strategies provide information and advice on what is problem drug use and its consequences, how to approach a person about their problem drug use, tips for effective communication, what to do if the person is unwilling to change their drug use, what to do if the person does (or does not) want professional help, what are drug-affected states and how to deal with them, how to deal with adverse reactions leading to a medical emergency, and what to do if the person is aggressive.</p> <p>Conclusions</p> <p>The guidelines provide a consensus-based resource for community members who want to help someone with a drug problem. It is hoped that the guidelines will lead to better support and understanding for those with problem drug use and facilitate engagement with professional help.</p

An International Comparison of the Effect of Policy Shifts to Organ Donation Following Cardiocirculatory Death (DCD) on Donation Rates After Brain Death (DBD) and Transplantation Rates

During the past decade an increasing number of countries have adopted policies that emphasize donation after cardiocirculatory death (DCD) in an attempt to address the widening gap between the demand for transplantable organs and the availability of organs from donation after brain death (DBD) donors. In order to examine how these policy shifts have affected overall deceased organ donor (DD) and DBD rates, we analyzed deceased donation rates from 82 countries from 2000–2010. On average, overall DD, DBD and DCD rates have increased over time, with the proportion of DCD increasing 0.3% per year (p = 0.01). Countries with higher DCD rates have, on average, lower DBD rates. For every one-per million population (pmp) increase in the DCD rate, the average DBD rate decreased by 1.02 pmp (95% CI: 0.73, 1.32; p<0.0001). We also found that the number of organs transplanted per donor was significantly lower in DCD when compared to DBD donors with 1.51 less transplants per DCD compared to DBD (95% CI: 1.23, 1.79; p<0.001). Whilst the results do not infer a causal relationship between increased DCD and decreased DBD rates, the significant correlation between higher DCD and lower DBD rates coupled with the reduced number of organs transplanted per DCD donor suggests that a national policy focus on DCD may lead to an overall reduction in the number of transplants performed

Consanguinity and rare mutations outside of MCCC genes underlie nonspecific phenotypes of MCCD

Purpose: 3-Methylcrotonyl-CoA carboxylase deficiency (MCCD) is an autosomal recessive disorder of leucine catabolism that has a highly variable clinical phenotype, ranging from acute metabolic acidosis to nonspecific symptoms such as developmental delay, failure to thrive, hemiparesis, muscular hypotonia, and multiple sclerosis. Implementation of newborn screening for MCCD has resulted in broadening the range of phenotypic expression to include asymptomatic adults. The purpose of this study was to identify factors underlying the varying phenotypes of MCCD. Methods: We performed exome sequencing on DNA from 33 cases and 108 healthy controls. We examined these data for associations between either MCC mutational status, genetic ancestry, or consanguinity and the absence or presence/specificity of clinical symptoms in MCCD cases. Results: We determined that individuals with nonspecific clinical phenotypes are highly inbred compared with cases that are asymptomatic and healthy controls. For 5 of these 10 individuals, we discovered a homozygous damaging mutation in a disease gene that is likely to underlie their nonspecific clinical phenotypes previously attributed to MCCD. Conclusion: Our study shows that nonspecific phenotypes attributed to MCCD are associated with consanguinity and are likely not due to mutations in the MCC enzyme but result from rare homozygous mutations in other disease genes

Adaptive Refinements in BEM

Accuracy estimates and adaptive refinements is nowadays one of the main research topics in finite element computations [6,7,8, 9,11].Its extension to Boundary Elements has been tried as a means to better understand its capabilities as well as to impro ve its efficiency and its obvious advantages. The possibility of implementing adaptive techniques was shown [1,2] for h-conver gence and p-convergence respectively. Some posterior works [3,4 5,10] have shown the promising results that can be expected from those techniques. The main difficulty is associated to the reasonable establishment of “estimation” and “indication” factors related to the global and local errors in each refinement. Although some global measures have been used it is clear that the reduction in dimension intrinsic to boundary elements (3D→2D: 2D→1D) could allow a direct comparison among residuals using the graphic possibilities of modern computers and allowing a point-to-point comparison in place of the classical global approaches. Nevertheless an indicator generalizing the well known Peano’s one has been produced