Influence of Diet and Genetic Strain on Desirable Flavors in Farm-Raised Catfish.

This farm-raised catfish quality study measured desirable flavor attributes, Chickeny, Nutty, Fat Complex, Corn, Sweet, with the objective to determine effects of feed ingredients, time-on-diet and genetic strain. Overall flavor impact differences were tested by triangle tests. The diets were Casein-base reference feed by itself or partially substituted with: 10% catfish meal, 10% meat and bone meal, 10% rice bran, or 3% menhaden oil and commercial feed formulation. Fish were grown indoors to minimize environmental flavors for 70, 160, 210 and 318 days. The genetic strains evaluated were channel, albino channel, blue, hybrid channel x blue, and black bullhead catfish. These fish were stocked and fed commercial fingerling feed for no less than 14 days. Blended individual fish samples were prepared for trained descriptive (n = 9) and triangle test panels (n = 18). The descriptive analyses showed no significant differences due to feed, time on-diet, or genetic strain. Differences found were not greater than the minimum detectable differences set by a preliminary power analysis. Triangle tests revealed black bullheads to be different from all other genetic strains, as was expected. Commercial reference catfish were found to be different possibly because of a slight off-flavor that was a cue to panelists. Inconsistent overall impact in descriptive and triangle evaluations indicates small differences exist that would likely be at the same intensity or masked by common flavors from pond influences. Fillet fat content was consistent with other reports, and the lack of flavor differences with increasing time-on-diet suggested that longer growing time to develop flavor is not warranted. This study supports producer practices of least-cost formulation. Odor analysis by gas chromatography-olfactometry was performed on selected treatments to explore patterns of impact odorants from catfish oil extracts. An intensity method was performed by four panelists. Twenty compounds were found that met the criteria that at least one panelist rated a moderate intensity or higher. All compounds have been found in animal and vegetative products. The three most consistent stimuli perceived were green grassy, mushroom and mothballs. Canonical correlation of the reliability of odor compound data to predict flavor-by-mouth characteristics did not find any significant relationships

Youth work: a systematic map of the research literature.

This is the first systematic international map of youth work research. It provides a unique resource for investigating the content of youth work, how it is delivered and the terms in which it is assessed, both in formal evaluations of its impact and by children and young people themselves. It provides a valuable basis for developing an evidence-informed approach to policy and practice

Exploring the effectiveness of a screening measure to identify subtle cognitive and functional problems in a sample of acquired brain injury patients admitted to a neurological hospital in the UK: A feasibility study.

Patients considered asymptomatic after acquired brain injury (ABI) may be exhibiting undetected cognitive deficits which can lead to problems with everyday tasks. Current screening tools focus on cognitive deficits and not functional impact. This cross-sectional feasibility study aimed to explore the use of a bedside screening tool: Cognitive Functional Performance Measure (CFPM). Drawing on occupational therapy theory and principles, the CFPM offers the multi-disciplinary team a unique tool to trigger referral to occupational therapy. A sample of patients with ABI (n = 34) were recruited and their CFPM scores were compared with scores on the Montreal Cognitive Assessment (MoCA) and the Kettle Test. Spearman’s rank and Chi-square were used to analyse the data. A moderate correlation was found between the MoCA and CFPM. There was no significant association between the type of ABI and performance on the CFPM. The unique design of the CFPM offers an alternative to existing screening tools, placing emphasis on the identification of cognitive impairment and functional deficits with the ultimate goal to develop a tool that is ecologically valid. Further studies exploring the feasibility and validity of the CFPM is recommended

'It's not one size fits all':A qualitative study of patients' and healthcare professionals' views of self-management for bronchiectasis

Background Bronchiectasis is a chronic respiratory condition that impacts significantly on individuals and healthcare services. Self-management is recommended in clinical guidelines for bronchiectasis as an intervention to enable patients to manage their condition, yet there is little evidence to support it.Methods Three face to face focus groups (17 adults with bronchiectasis) were conducted at three National Health Service (NHS) sites in North West England. Additionally, semi-structured telephone interviews were undertaken with 11 healthcare professionals (HCPs), including doctors, nurses and physiotherapists. Thematic analysis identified common themes and occurrences verified by independent audit.Findings Four common overarching themes were identified: the meaning of self-management; benefits; barriers and influencers to self-management; subthemes varied. Both groups recognised component interventions. Patients highlighted that self-management enabled them to learn what works and moderate behaviour. Aspects of delivery and structure were important to HCPs but a ‘make do’ culture was evident. Benefits for both groups included empowering patients. Common barriers for patients were time, mood and lack of access to support which could mitigate engagement with self-management. HCPs identified barriers including patient characteristics and lack of resources. Influencers for patients were peer, carer and psychosocial support, for HCPs influencers were individual patient attributes, including ability and motivation, and HCP characteristics such as knowledge and understanding about bronchiectasis.Summary This is the first study to explore patients’ and HCPs’ views of self-management for bronchiectasis. The need for an individual, flexible and responsive self-management programme specific to bronchiectasis was evident. Personal characteristics of patients and HCPs could affect the uptake and engagement with self-management and HCPs knowledge of the disease is a recognised precursor to effective self-management. The study identified key aspects for consideration during development, delivery and sustainability of self-management programmes and findings suggest that patients’ psychosocial and socioeconomic circumstances may affect adoption and activation of self-management behaviours

Research priorities for respiratory nursing:A UK-wide delphi study

Respiratory nurses make a significant contribution to the delivery of respiratory healthcare, but there is a dearth of nurse-led, practice-focused, published research. Using a modified three-round Delphi, this study sought to identify research priorities for respiratory nursing to inform a national research strategy. Study information and the survey link were sent electronically to members of UK professional respiratory organisations. Round 1 had 78 items across 16 topics, informed by a systematic literature review. Respondents suggested additional items which were content analysed to inform Round 2. Respondents rated all items and ranked the topics in all rounds. To ensure rigour, rounds had an explicit focus with pre-determined criteria for consensus (70%). In total, 363 responses were received across Rounds 1, 2 and 3 (n=183, 95 and 85, respectively). The top five research priorities were: 1) “Patient understanding of asthma control”; 2) “The clinical and cost-effectiveness of respiratory nurse interventions”; 3) “The impact of nurse-led clinics on patient care”; 4) “Inhaler technique”; and 5) two topics jointly scored: “Prevention of exacerbations” and “Symptom management”. With potential international significance, this is the first UK study to identify research priorities for respiratory nursing, providing direction for those planning or undertaking research

Epidemiology of plasmid lineages mediating the spread of extended-spectrum beta-lactamases among clinical Escherichia coli

The prevalence of extended-spectrum beta-lactamases (ESBLs) among clinical isolates of Escherichia coli has been increasing, with this spread driven by ESBL-encoding plasmids. However, the epidemiology of ESBL-disseminating plasmids remains understudied, obscuring the roles of individual plasmid lineages in ESBL spread. To address this, we performed an in-depth genomic investigation of 149 clinical ESBL-like E. coli isolates from a tertiary care hospital. We obtained high-quality assemblies for 446 plasmids, revealing an extensive map of plasmid sharing that crosses time, space, and bacterial sequence type boundaries. Through a sequence-based network, we identified specific plasmid lineages that are responsible for the dissemination of major ESBLs. Notably, we demonstrate that IncF plasmids separate into 2 distinct lineages that are enriched for different ESBLs and occupy distinct host ranges. Our work provides a detailed picture of plasmid-mediated spread of ESBLs, demonstrating the extensive sequence diversity within identified lineages, while highlighting the genetic elements that underlie the persistence of these plasmids within the clinical E. coli population