Anesthesia management for ALS and WPW

WOS: 000435650500021Epidural anesthesia can provide anesthesia and analgesia for unilateral or bilateral lower extremity surgery and is associated with a low complication rate. We present our epidural anaesthetic management of a patient with both Amyotrophic lateral sclerosis (ALS) and Wolff-Parkinson-White (WPW) syndrome after intertrochanteric femur fracture surgery. It should be kept in mind that the choice of correct anaesthetic method in such patients with complicated neurological, pulmonary, and cardiac symptoms will significantly reduce postoperative mortality and morbidity

The relationship between pressure ulcers and palliative care patient caregivers’ knowledge levels

Introduction: The need for care and palliative care increased with the prolongation of mean life expectancy. One of the most frequently observed symptoms in palliative care is pressure ulcers. We aimed to measure the knowledge levels of caregivers serving hospitalized patients in the palliative care service and to investigate their relationship with socio-demographic characteristics, especially the knowledge levels.Methods: This study enrolled 109 people providing care to patients who received inpatient palliative care between November 1 and December 31, 2018. A questionnaire including questions about socio-demographic characteristics and knowledge about pressure ulcers was administered. The pressure ulcer status of the patients was evaluated by a physician. Results: The mean age of the caregivers was 49.49 ± 10.91 years (22-69). The mean knowledge level of the participants was 70.09 ± 18.58%. The minimum score was 0, and the maximum score was 100. It was found that 62.4% of the caregivers received information about pressure ulcers in the past, 30.3% of them didn’t receive any information about pressure ulcers, and 7.3% received partial information.Conclusion: The knowledge level of the caregivers who were informed about pressure ulcers was significantly higher than those who were not informed (p=0.005). Therefore, we think that continuous education has an essential place in preventing pressure ulcers

Advantages of nanoscale bioactive glass as inorganic filler in alginate hydrogels for drug delivery and biofabrication

Sodium alginate is a natural biocompatible polymer obtained from brown algae which has found numerous biomedical applications. Inorganic fillers, such as nanosized bioactive glass (nBG) particles, are well known for their outstanding properties in terms of being osteoconductive and osteoinductive and therefore finding application in bone tissue engineering. In this study, the impact of nBG particles on alginate hydrogels was investigated for applications of the composite hydrogel in biofabrication and drug delivery. The influence of nBG particles on properties such as printability, drug release ability and bioactivity (capability to form a hydroxyapatite (HAp) layer on the surface of nBG-alginate capsules) was studied. In vitro cell studies proved high cell viability of all inks. Due to the presence of nBG particles, more precise printed grids and pore sizes were achieved. Moreover, a decrease of the release of the model drug icariin in the presence of nBG particles was determined. The formation of a HAp layer on the surface of nBG-alginate capsules was assessed by FTIR, XRD and SEM. Overall, the addition of nBG particles into alginate hydrogels led to the improvement of the relevant properties investigated. Therefore, nBG-alginate systems should gain more attention for 3 D bioprinting and drug delivery approaches. Supplemental data for this article is available online at https://doi.org/10.1080/26889277.2022.2039078

Hepatoprotective actions of melatonin by mainly modulating oxidative status and apoptosis rate in lipopolysaccharide-induced liver damage

Aim: One of the serious complications of sepsis is liver damage and liver failure. This study aimed to evaluate the protective and therapeutic potential of melatonin in rats with lipopolysaccharide-induced sepsis. Main methods: Female Spraque–Dawley rats received single a dose of 7.5 mg/kg lipopolysaccharide in saline to create a 24-h sepsis model. One of the other groups received melatonin at a dose of 10 mg/kg/day beginning 1 week before sepsis induction to the end of the experiment. The melatonin group received the same doses of melatonin for the same duration but not lipopolysaccharide. The vehicle group received the same doses of saline, the vehicle of melatonin, for the same duration. Twenty-four hours after the last injection, the rats were decapitated. By appropriate histochemical, immunohistochemical, biochemical, and molecular techniques, anti-necrotic, anti-apoptotic, anti-necroptotic, anti-inflammatory, and antioxidant effects of melatonin were assessed. Key findings: Lipopolysaccharide has disrupted liver functions by inducing oxidative stress, inflammation, necrotic, apoptotic, and necroptotic cell death, thus disrupting liver functions. Melatonin was found to be beneficial in terms of inhibiting the intrinsic pathway of apoptosis and tissue oxidant levels, stimulating tissue antioxidant enzyme levels, and restoring hepatocyte functions. Significance: Melatonin, at those doses and duration, was found to be hepatoprotective by mainly modulating oxidative status and apoptosis rate, however, failed to significantly reduce histopathological damage. We suggest that longer-term melatonin administration may produce anti-inflammatory and anti-necrotic effects as well. © 2023 Informa UK Limited, trading as Taylor & Francis Group

3D printed poly(hydroxybutyrate-co-hydroxyvalerate)—45S5 bioactive glass composite resorbable scaffolds suitable for bone regeneration

Abstract: 3D printing for tissue engineering requires biomaterials with mechanical and biological properties suitable for both tissue regeneration and the printing process. A filament made of poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) combined with 45S5 Bioglass (BG) was used to print 3D scaffolds by fused deposition modeling (FDM). Chemical treatment of BG particles with chlorotrimethylsilane (CTMS) improved the ductility of the extruded filaments and allowed excellent printability. Controlling the printing parameter infill density (I%), from 20 to 90%, scaffolds were obtained with interconnected pores and channel sizes in the 100–800 µm range and exhibiting tensile modulus from 0.25 to 1.36 GPa. PHBV + BG scaffolds and PHBV scaffolds coated with CTMS treated BG particles, as a model of a rough and biologically active coating, showed no cytotoxic effects, and cells preferred the scaffolds containing BG in terms of cell spreading. Mechanical and biological properties of the scaffolds were similar to those of the extracellular matrix (ECM) of trabecular bone. Graphic abstract: [Figure not available: see fulltext.]Fil: Aráoz, Silvina Beatriz. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Instituto de Tecnologias Emergentes y Ciencias Aplicadas. - Universidad Nacional de San Martin. Instituto de Tecnologias Emergentes y Ciencias Aplicadas.; Argentina. Universidad Nacional de San Martin. Escuela de Ciencia y Tecnologia. Laboratorio de Biomateriales, Biomecanica y Bioinstrumentacion.; ArgentinaFil: Karakaya, Emine. Universitat Erlangen-Nuremberg; AlemaniaFil: González Sánchez Wusener, Ana Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; ArgentinaFil: Detsch, Rainer. Universitat Erlangen-Nuremberg; AlemaniaFil: Bizzotto, Juan Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; ArgentinaFil: Gueron, Geraldine. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; ArgentinaFil: Boccaccini, Aldo R.. Universitat Erlangen-Nuremberg; AlemaniaFil: Hermida, Elida Beatriz. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Instituto de Tecnologias Emergentes y Ciencias Aplicadas. - Universidad Nacional de San Martin. Instituto de Tecnologias Emergentes y Ciencias Aplicadas.; Argentina. Universidad Nacional de San Martin. Escuela de Ciencia y Tecnologia. Laboratorio de Biomateriales, Biomecanica y Bioinstrumentacion.; Argentin

An Inverse Thermogelling Bioink Based on an ABA-Type Poly(2-oxazoline) Amphiphile

Hydrogels are key components in several biomedical research areas such as drug delivery, tissue engineering, and biofabrication. Here, a novel ABA-type triblock copolymer comprising poly(2-methyl-2-oxazoline) as the hydrophilic A blocks and poly(2-phenethyl-2-oxazoline) as the aromatic and hydrophobic B block is introduced. Above the critical micelle concentration, the polymer self-assembles into small spherical polymer micelles with a hydrodynamic radius of approx 8-8.5 nm. Interestingly, this specific combination of hydrophilic and hydrophobic aromatic moieties leads to rapid thermoresponsive inverse gelation at polymer concentrations above a critical gelation concentration (20 wt %) into a macroporous hydrogel of densely packed micelles. This hydrogel exhibited pronounced viscoelastic solid-like properties, as well as extensive shear-thinning, rapid structure recovery, and good strain resistance properties. Excellent 3D-printability of the hydrogel at lower temperature opens a wide range of different applications, for example, in the field of biofabrication. In preliminary bioprinting experiments using NIH 3T3 cells, excellent cell viabilities of more than 95% were achieved. The particularly interesting feature of this novel material is that it can be used as a printing support in hybrid bioink systems and sacrificial bioink due to rapid dissolution at physiological conditions.Peer reviewe

Improving alginate printability for biofabrication: establishment of a universal and homogeneous pre-crosslinking technique

Many different biofabrication approaches as well as a variety of bioinks have been developed by researchers working in the field of tissue engineering. A main challenge for bioinks often remains the difficulty to achieve shape fidelity after printing. In order to overcome this issue, a homogeneous pre-crosslinking technique, which is universally applicable to all alginate-based materials, was developed. In this study, the Young’s Modulus after post-crosslinking of selected hydrogels, as well as the chemical characterization of alginate in terms of M/G ratio and molecular weight, were determined. With our technique it was possible to markedly enhance the printability of a 2% (w/v) alginate solution, without using a higher polymer content, fillers or support structures. 3D porous scaffolds with a height of around 5 mm were printed. Furthermore, the rheological behavior of different pre-crosslinking degrees was studied. Shear forces on cells as well as the flow profile of the bioink inside the printing nozzle during the process were estimated. A high cell viability of printed NIH/3T3 cells embedded in the novel bioink of more than 85% over a time period of two weeks could be observed

Calcium supplementation of bioinks reduces shear stress-induced cell damage during bioprinting

AbstractDuring bioprinting, cells are suspended in a viscous bioink and extruded under pressure through small diameter printing needles. The combination of high pressure and small needle diameter exposes cells to considerable shear stress, which can lead to cell damage and death. Approaches to monitor and control shear stress-induced cell damage are currently not well established. To visualize the effects of printing-induced shear stress on plasma membrane integrity, we add FM 1-43 to the bioink, a styryl dye that becomes fluorescent when bound to lipid membranes, such as the cellular plasma membrane. Upon plasma membrane disruption, the dye enters the cell and also stains intracellular membranes. Extrusion of alginate-suspended NIH/3T3 cells through a 200 µm printing needle led to an increased FM 1-43 incorporation at high pressure, demonstrating that typical shear stresses during bioprinting can transiently damage the plasma membrane. Cell imaging in a microfluidic channel confirmed that FM 1-43 incorporation is caused by cell strain. Notably, high printing pressure also impaired cell survival in bioprinting experiments. Using cell types of different stiffnesses, we find that shear stress-induced cell strain, FM 1-43 incorporation and cell death were reduced in stiffer compared to softer cell types and demonstrate that cell damage and death correlate with shear stress-induced cell deformation. Importantly, supplementation of the suspension medium with physiological concentrations of CaCl2 greatly reduced shear stress-induced cell damage and death but not cell deformation. As the sudden influx of calcium ions is known to induce rapid cellular vesicle exocytosis and subsequent actin polymerization in the cell cortex, we hypothesize that calcium supplementation facilitates the rapid resealing of plasma membrane damage sites. We recommend that bioinks should be routinely supplemented with physiological concentrations of calcium ions to reduce shear stress-induced cell damage and death during extrusion bioprinting

Ulusaldan Küresele: Popülizm, Demokrasi, Güvenlik Konferansı

Öngörülmesi giderek güçleşen, sarsıntılı ve savrulmalı zamanlardan geçiyoruz. İkinci Dünya Savaşı ve Soğuk Savaş ortak deneyimleri sonrasında 1950’lerden ve 1990’lardan itibaren demokratik sistemlerin peş peşe dalgalarla meşrulaşacağı, yaygınlaşacağı ve güçleneceği öngörüsü hakimdi. Ancak son yıllarda yaşanan bazı gelişmelerle demokrasilerin geleceği tekrar sorgulanmaya başladı. Gerek 11 Eylül ile başlayan ve IŞİD ile devam eden ve şiddet içeren İslamcı radikalizm, gerek Batı demokrasilerinde popülist radikal sağ hareketlerin ve beyaz ırkçı grupların yükselişi ve iktidara gelişi, bir yandan güvenlik-özgürlük ikileminin demokrasi dengesini bozdu, bir yandan da hem demokratik sistemlerin hem dünya barışının geleceğini bizi tekrar sorgular, sorgulatır hale getirdi. Demokrasileri bildiğimizi zannediyoruz, ama demokrasiler ile ilgili daha öğrenmemiz gereken çok şey var. Demokrasi kaderimiz de geleceğimiz de olmak zorunda değil belki de. Ya da belki yanlış yerden soru sormaya başlıyoruz, belki demokrasi yerine yeni bir referansa ihtiyacımız var. Aslında demokrasileri çantada keklik görmeyip, sabırla büyütüp yeşertmek, geliştirmek, korumak, ileri safhalara taşımak ve bizden sonraki nesillere aktarmak bir sorumluluk, ve bu sorumluluk bizlere ait. Popülizm, demokrasi, güvenlik kavramlarının her biri bugün sıkça ve yaygın olarak kullandığımız kavramlar olarak gündelik sohbetlerimizin içine kadar girmiş durumda. Bu yaygın kullanımlarına rağmen her bir kavram, üzerine düşünmeye, tartışmaya ve değerlendirmeye tekrar tekrar olanak verecek derinlikte. Her bir tartışma bir diğerini açarken, farklı gibi görünen bu kavramların birbirleriyle kesiştikleri zeminler bulmak mümkün. Popülist liderlerin politikaları bütün siyaset yapma biçimlerini kendine çeken ya da kendinden uzaklaştıran eksenler yaratarak her ikisini de aynı anda besleyebiliyor. Popülist politikaya angaje olan liderler ve grupların yanında bu politikaya karşı mücadele eden kişiler ve kitleler de yok değil, ancak kimi zaman bu kitleler eleştirdiği bu siyaset biçiminin kurucu öznesi haline de gelebiliyor. Bunun karşısında tabandan gelen demokratikleşme talepleri ve popülist siyasetle beraber kurumsallaşan diğer politika yapma biçimleri, demokrasi anlayışımızı farklı yönlere çekebiliyor. Bu demokratikleşme talepleri kimi zaman olumlu karşılıklar alsa da, kimi zaman devletlerin güvenlik politikaları ile etkisizleştirilmeye ve bastırılmaya çalışılıyor. Güvenlik politikalarının alanı günümüz teknolojisi sebebiyle o kadar genişledi ki, bu politikanın nesnesi haline gelmemiş varlık ve alan bulmak neredeyse mümkün değil. Ulusaldan Küresele: Popülizm, Demokrasi, Güvenlik konferansımız bu alanların kendine özgülüklerini göz önünde bulundururken, aralarındaki kesişimleri de ortaya koyan pek çok değerli sunuma ev sahipliği yaptı. Konferansın düzenlenmesinde emeği geçen herkese, ve bu bildiri kitabında tam metinleri ve özetleri bulunan bütün katılımcılarımıza çok teşekkür ederiz.Publisher's Versio

Tükenmişlik, iş stresi ve duygusal zeka arasındaki ilişki: akademisyenler üzerine bir araştırma

Tükenmişlik örgütsel ve sosyal yapılarda güncelliğini koruyarak üzerinde çalışılmaya devam edilen bir kavramdır. Tükenmişliğin bireylerde farklı seviye ve boyutlarda görülmesi bireylerin davranışlarını, iş yapış biçimlerini ve ilişki kurabilme yeteneklerini etkilemektedir. Çalışmada, adı tükenmişlik kavramı ile sık sık birlikte anılan iş stresi kavramına da yer verilerek bunların birbirleri üzerindeki artırıcı, azaltıcı, dengeleyici vb. gibi etkileri incelenmiştir. Kontrol altına alınmadığı durumlarda bireyin çalışma, aile ve sosyal yaşantısına olumsuz etkileri olabilecek bu kavramları dengede tutabilmek de bireyin sahip olduğu zekâ türü ile doğrudan ilişkilidir. Burada önem kazanan duygusal zekâ kavramının bireyin sahip olduğu duygularını aklının ışığında nasıl yönlendirdiği üzerinde durması olmuştur.İnsanlar küreselleşen dünya koşulları ile birlikte her geçen gün daha fazla çalışmak ve kazanmak zorunda olduklarını düşünür ve hissederler. Bir bakıma hayatta kalabilmek için çalışmak zorunda olan insan bunu yaparken çeşitli sorun, stres yaratan olay ve çözülmesi gereken problemle karşılaşır. Bunların üstesinden gelebilmek için sahip olduğu iç sermayesini yerinde ve verimli kullanmak zorundadır. Bu yapılmadığı takdirde küçük streslerle baş gösteren olay ve durumlar bireyi tükenmişliğin ileriki boyutlarına kadar sürükleyebilir. İç sermaye olarak adlandırdığımız donanımlar sağduyulu bir insanın karakteristik özellikleri üzerinden de yorumlanabilir. Tez çalışmamızda bu materyalleri duygusal zekâ başlığı altında toplamış bulunmaktayız. Bireyin stresini ve tükenme durumunu fark edebilmesi, yönetebilmesi daha genel bir ifade ile tüm bu süreçleri kontrol edebilmesi için sadece zekâsına veya duygularına değil ikisine birden ihtiyaç duyduğu tezimizin temelini oluşturmaktadır. Birçok araştırmacının çalışmasına konu edindiği bu kavramların birbirleri ile etkileşim durumları ise bizim tezimize konu olmuştur.Bu tez çalışmasında veri toplama yöntemi olarak anket tekniğinden yararlanılmış, Türkiye’deki üniversitelerde görev yapan akademisyenlerin duygusal zekâ, iş stresi ve tükenmişlik düzeylerini ölçmek amaçlanmıştır. Aynı zamanda bu değişkenlerin birbirleri üzerindeki etkileri de tezimize konu olmuştur. Özellikle duygusal zekânın ve stresin ayrı ayrı tükenmişlik üzerindeki etkileri çalışmanın temelini oluşturmaktadır. Özel ve kamu olmak üzere Türkiye geneli yaklaşık 160.000 akademisyenin evren olarak seçildiği çalışmada farklı bölgelerden, farklı üniversite ve unvanlardan ankete katılım sağlanmıştır.--------------------Burnout is a concept that continues to be worked on in organizational and social structures. The burnout has been seen in individuals at different levels and dimensions which affects the behaviors, behaviors and relationships of individuals. In this study, the concept of burnout and frequently referred to the concept of business stress are discussed and their effects on each other such as enhancing, reducing and stabilizing are examined. In situations where it is not controlled, keeping these concepts in balance, which may have negative effects on the work, family and social life of the individual, is directly related to the type of intelligence that an individual has. The concept of emotional intelligence, which gained importance here, has been to emphasize how the individual's feelings are directed in the light of his mind.People think and feel that they have to work and gain more and more every day with the conditions of the globalizing world. A person who has to work in order to survive is faced with various troubles, stressful events and the problem to be solved. They have to use the internal capital that they have in order to get there from above and efficiently. If this is not done, events and situations that start with small stresses can drag them to the later stages of individual exhaustion. The equipment we call internal capital can also be interpreted through the characteristics of a common man. In our thesis we are collecting these materials under the title of emotional intelligence.To be able to recognize the stress and exhaustion of the individual and to manage it in a more general way can only control both these processes. The interaction of these concepts, which many researchers are working on, is the subject of my thesis.This thesis work has been utilized as a method of data collection in survey techniques and aimed to measure levels of emotional intelligence job stress and burnout of academics working at universities in Turkey. At the same time, the effects of these variables on each other have been the subject of our thesis. Especially the effects of emotional intelligence and stress on burnout constitute the basis of the study. Turkey in general, including about 160,000 public and private research academics from different regions of the universe as the work is selected, titles and those from different universities participated in the survey