Anderson v. Edwards: Can Two Live More Cheaply Than One? The Effect of Cohabitation on AFDC Grants

This note will first discuss the background of the AFDC program and how it is regulated by the federal and state governments. A discussion of several lower federal and state court decisions which have dealt with the issue presented to the United States Supreme Court in Anderson v. Edwards will follow. Next, this note will examine the Court\u27s analysis and holding in Anderson. The note concludes with the author\u27s assessment as to why the holding in Anderson was correct

Anderson v. Edwards: Can Two Live More Cheaply Than One? The Effect of Cohabitation on AFDC Grants

This note will first discuss the background of the AFDC program and how it is regulated by the federal and state governments. A discussion of several lower federal and state court decisions which have dealt with the issue presented to the United States Supreme Court in Anderson v. Edwards will follow. Next, this note will examine the Court\u27s analysis and holding in Anderson. The note concludes with the author\u27s assessment as to why the holding in Anderson was correct

Structure and substrate selectivity of the 750-kDa α6β6 holoenzyme of geranyl-CoA carboxylase.

Geranyl-CoA carboxylase (GCC) is essential for the growth of Pseudomonas organisms with geranic acid as the sole carbon source. GCC has the same domain organization and shares strong sequence conservation with the related biotin-dependent carboxylases 3-methylcrotonyl-CoA carboxylase (MCC) and propionyl-CoA carboxylase (PCC). Here we report the crystal structure of the 750-kDa α6β6 holoenzyme of GCC, which is similar to MCC but strikingly different from PCC. The structures provide evidence in support of two distinct lineages of biotin-dependent acyl-CoA carboxylases, one carboxylating the α carbon of a saturated organic acid and the other carboxylating the γ carbon of an α-β unsaturated acid. Structural differences in the active site region of GCC and MCC explain their distinct substrate preferences. Especially, a glycine residue in GCC is replaced by phenylalanine in MCC, which blocks access by the larger geranyl-CoA substrate. Mutation of this residue in the two enzymes can change their substrate preferences

Adsorción de contaminantes en sedimentos del Holoceno de la región de La Plata

Técnicas geológicas, geoquímicas y geofísicas fueron aplicadas a los fines de determinar la presencia y concentración de metales pesados en sedimentos de los cursos de agua de la región de La Plata. Estos arroyos, que atraviesan el casco urbano, han sido entubados en su gran mayoría, empero en los sectores bajos y en la planicie costera corren a cielo abierto por canales.Con el crecimiento urbanístico y poblacional, los cursos de agua son también receptores de desechos urbanos, industriales y agropecuarios. Interpretamos que las arcillas de los sedimentos de la región retienen con mayor facilidad (adsorción) los contaminantes, que si estos materiales fueran de granulometría gruesa. A partir de este supuesto se realizó la investigación procurando establecer la relación existente entre los distintos parámetros utilizados, los metales pesados, la variación del contenido en materia orgánica y la concentración de óxidos de hierro. Esto último debido particularmente a que óxidos y oxyhidróxidos de hierro, en asociación con arcillas esmectitas, coadyuvan en el proceso de adsorción de contaminantes. El área de estudio se ubica en 34º 50´ y 35º 2´ Lat. S, 57º 45´ y 58º 5´ Long. W.Geological, geochemical and geophysical techniques were applied for the purposes of determining the presence and concentration of heavy metals in streams sediments of La Plata region. These streams, passing through the town, have been tuned in its vast majority, but in low areas as on the coastal plain they run in open channels.With the population and urban growth, water courses are also recipients of urban, industrial and agricultural wastes. We interpret that clay minerals of the sediments of the streams retain more easily (adsorption) pollutants than sediments of coarse grain size. From this assumption a research trying to establish the relationship between the parameters used, heavy metals and variation of the organic matter was performed. The concentration of total iron oxides was also taken into account considering that iron oxides and oxyhydroxides assist in the process of adsorption of contaminants in association with smectite clays. The area of study is located between 34º 50´- 35º 2´ Lat. S and 57º 45´- 58º 5´ Long. W

Marker based Thermal-Inertial Localization for Aerial Robots in Obscurant Filled Environments

For robotic inspection tasks in known environments fiducial markers provide a reliable and low-cost solution for robot localization. However, detection of such markers relies on the quality of RGB camera data, which degrades significantly in the presence of visual obscurants such as fog and smoke. The ability to navigate known environments in the presence of obscurants can be critical for inspection tasks especially, in the aftermath of a disaster. Addressing such a scenario, this work proposes a method for the design of fiducial markers to be used with thermal cameras for the pose estimation of aerial robots. Our low cost markers are designed to work in the long wave infrared spectrum, which is not affected by the presence of obscurants, and can be affixed to any object that has measurable temperature difference with respect to its surroundings. Furthermore, the estimated pose from the fiducial markers is fused with inertial measurements in an extended Kalman filter to remove high frequency noise and error present in the fiducial pose estimates. The proposed markers and the pose estimation method are experimentally evaluated in an obscurant filled environment using an aerial robot carrying a thermal camera.Comment: 10 pages, 5 figures, Published in International Symposium on Visual Computing 201

Application of computational methods in the assessment of the aeroelastic response of cable supported bridges

En este trabajo se muestra el potencial de los métodos computacionales para analizar la respuesta frente a la acción del viento de los puentes soportados por cables, y se evalúa su capacidad para sustituir a las campañas experimentales en túnel de viento, particularmente en las fases iniciales del diseño. Se ha tomado como caso de estudio el diseño preliminar propuesto para el proyecto real de un puente atirantado continuo con 2 vanos de 650 m y sección transversal en cajón. Así, se han evaluado numéricamente los coeficientes aerodinámicos de la sección transversal y se ha simulado la respuesta no estacionaria frente al desprendimiento de torbellinos utilizando software comercial de mecánica de fluidos computacional. Además, se ha analizado, mediante un código propio, la respuesta de la estructura frente a los fenómenos del flameo y el bataneo utilizando la metodología híbrida y adoptando valores de las funciones experimentales de flameo pertenecientes a una sección transversal de geometría similar. Los resultados computacionales obtenidos se han validado comparándolos con datos experimentales de puentes semejantes publicados por otros autores. Se ha comprobado que el conjunto de métodos adoptados ofrece resultados fiables con costes moderados, por lo que el planteamiento descrito es muy atractivo en la fase inicial del proyecto de puentes de gran vano o en trabajos de diseño conceptual.The possibilities of computational methods for assessing the response of cable supported bridges under wind action are considered in this work. The main objective is to study the possibilities of substituting wind tunnel campaigns by computer based analyses, particularly at the early design stage. The preliminary proposed design for a continuous cable-stayed bridge with two main spans of 650 m and a single box girder deck has been considered as a case study. The force coefficients of the deck cross-section have been computed and the unsteady response associated to vortex-shedding has been simulated using CFD commercial software. Furthermore, an in-house piece of software has been employed to obtain the response for flutter and buffeting phenomena adopting the hybrid approach; with that purpose the experimental flutter functions of a similar box girder deck were adopted. The computational results have been validated by comparison with similar experimental results published by other researchers. It has been verified that the set of adopted methods offers reliable results with moderate costs; therefore, the proposed approach is very suitable at the early design stage of long span bridges or at conceptual design works.Peer Reviewe

ZAP's stress granule localization is correlated with its antiviral activity and induced by virus replication.

Cellular antiviral programs encode molecules capable of targeting multiple steps in the virus lifecycle. Zinc-finger antiviral protein (ZAP) is a central and general regulator of antiviral activity that targets pathogen mRNA stability and translation. ZAP is diffusely cytoplasmic, but upon infection ZAP is targeted to particular cytoplasmic structures, termed stress granules (SGs). However, it remains unclear if ZAP's antiviral activity correlates with SG localization, and what molecular cues are required to induce this localization event. Here, we use Sindbis virus (SINV) as a model infection and find that ZAP's localization to SGs can be transient. Sometimes no apparent viral infection follows ZAP SG localization but ZAP SG localization always precedes accumulation of SINV non-structural protein, suggesting virus replication processes trigger SG formation and ZAP recruitment. Data from single-molecule RNA FISH corroborates this finding as the majority of cells with ZAP localization in SGs contain low levels of viral RNA. Furthermore, ZAP recruitment to SGs occurred in ZAP-expressing cells when co-cultured with cells replicating full-length SINV, but not when co-cultured with cells replicating a SINV replicon. ZAP recruitment to SGs is functionally important as a panel of alanine ZAP mutants indicate that the anti-SINV activity is correlated with ZAP's ability to localize to SGs. As ZAP is a central component of the cellular antiviral programs, these data provide further evidence that SGs are an important cytoplasmic antiviral hub. These findings provide insight into how antiviral components are regulated upon virus infection to inhibit virus spread

Meandering periods and asymmetries in light curves of Miras: Observational evidence for low mass-loss rates

Some Miras -- long-period variables in late evolutionary stages -- have meandering pulsation periods and light curve asymmetries, the causes of which are still unclear. We aim to understand better the origin of these phenomena by investigating a sample of solar-neighbourhood Miras. We characterised this group of stars and related their variability characteristics to other stellar parameters. We analysed observations from several databases to obtain light curves with maximum time span and temporal coverage for a sample of 548 Miras. We determined their pulsation period evolution over a time span of many decades, searched for changes in the periods, and determined the amplitude of the period change. We also analysed the Fourier spectra with respect to possible secondary frequency maxima. The sample was divided into two groups with respect to the presence of light curve bumps. IR colours and indicators of the third dredge-up were collected to study the sample stars' mass-loss and deep mixing properties. Our analysis revealed one new star, T~Lyn, with a continuously changing period. The group of Miras with meandering period changes is exclusively made up of M-type stars. The Fourier spectra of the meandering period Miras have no prominent additional peaks, suggesting that additional pulsation modes are not the cause of the meandering periods. We confirm that bumps are more common among S and C Miras and show, for the first time, that Miras with bumps have lower mass-loss rates than those with regular, symmetric light curves. Also Miras with meandering period changes have relatively little mass loss. We conclude that Miras with strongly changing periods or asymmetries in their light curves have relatively low dust mass-loss rates. Meandering period changes and light curve asymmetries could be connected to He-shell flashes and third dredge-up episodes.Comment: 13 pages (plus 13 pages Appendix), 14 Figures, accepted for publication in A&