Dispositivo para la detección de una única molécula en situación de nanoconfinamiento y en régimen dinámico, procedimiento de fabricación y uso

El objeto de la presente invención está relacionado con un dispositivo que integra estructuras de cristal fotónico y micro-nanocanales para la manipulación y detección de moléculas de ADN y otras moléculas con marcadores luminiscentes. El dispositivo, basado en la capacidad de las guías fotónicas de direccionar las ondas electromagnéticas, concentra la señal de emisión de la molécula en respuesta a una señal de excitación en una dirección dada. Son igualmente objetos de la presente invención el procedimiento de fabricación del dispositivo y el uso del mismo para la detección de una única molécula en situación de nanoconfinamiento y en régimen dinámico.Peer reviewedConsejo Superior de Investigaciones Científicas, Fundación TeknikerR Informe sobre el estado de la técnica publicado separadament

Thermal-nanoimprint lithography for perylenediimide-based distributed feedback laser fabrication

In the present work thermal-nanoimprint lithography of various commercial thermoplastic resists as matrixes for perylenediimides (PDIs) has been studied. This fabrication method reduces the number of fabrication steps, and therefore, the cost of the obtained distributed feedback (DFB) lasers. The optical properties of these devices are analyzed, aiming to optimize their performance.Spanish Government (MINECO) and the European Community (FEDER) through grants MAT2008-06648-C02 and MAT-2011-28167-C02. Manuel G. Ramírez is supported by a CSIC fellowship within the program JAE

Real-Time Label-Free Surface Plasmon Resonance Biosensing with Gold Nanohole Arrays Fabricated by Nanoimprint Lithography

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Optofluidic chips with nanochannels for dynamic molecular detection using enhanced fluorescence

The fabrication of a novel optofluidic chip using nanochannels optimized for DNA-stretched molecules and optical detection by enhanced fluorescence is reported. The chips are composed of a series of microchannels that allow the transport of molecules in the femto-liter per second inside a fluid or gas. The nanochannels are surrounded by a photonic crystal structure to enhance the emission of fluorescent light from the molecules, which can travel along the nanochannel, allowing for enhanced optical detection of the molecules in motion. The photonic crystal structure provides an enhancement up to 2.5 times in the light emitted from fluorescent molecules inside the nanochannels which increases to around 250 when normalized to the area of the nanochannels emitting fluorescence. The results may help to the detection of fluorescent molecules (like marked-DNA) in series by speeding it and allowing the use of less sophisticated equipment.This work was partially supported by Basque Government by Etortek 2013 Grant No. IE13–360 and by Spanish MINECO by Grants TEC2014-54449-C3-3-R and PCIN-2013-179.Peer reviewe

Cartridge, device and method for detecting, capturing, identifying and counting circulating tumour cells

A cartridge, device and method for detecting, capturing, identifying and counting circulating tumour cells, specifically relating to a cartridge which enables the capture and optionally recovery of circulating tumour cells (CTCs), a device for detecting, identifying and counting CTCs and a method for detecting, identifying and counting CTCs.Solicitud: PCT/EP2018/070724 (31.07.2018)Nº de Publicación: WO2019/025437 (07.02.2019