110 research outputs found
Shot Noise in Digital Holography
We discuss on noise in heterodyne holography in an off-axis configuration. We
show that, for a weak signal, the noise is dominated by the shot noise on the
reference beam. This noise corresponds to an equivalent noise on the signal
beam of one photoelectron per pixel, for the whole sequence of images used to
build the digital hologram
Imaging of a vibrating object by Sideband Digital Holography
We obtain quantitative measurements of the oscillation amplitude of vibrating
objects by using sideband digital holography. The frequency sidebands on the
light scattered by the object, shifted by n times the vibration frequency, are
selectively detected by heterodyne holography, and images of the object are
calculated for different orders n. Orders up to n=120 have been observed,
allowing the measurement of amplitudes of oscillation that are significantly
larger than the optical wavelength. Using the positions of the zeros of
intensity for each value of n, we reconstruct the shape of vibration the
object.Comment: 6 page
Imaging Gold Nanoparticles in Living Cells Environments using Heterodyne Digital Holographic Microscopy
This paper describes an imaging microscopic technique based on heterodyne
digital holography where subwavelength-sized gold colloids can be imaged in
cell environment. Surface cellular receptors of 3T3 mouse fibroblasts are
labeled with 40 nm gold nanoparticles, and the biological specimen is imaged in
a total internal reflection configuration with holographic microscopy. Due to a
higher scattering efficiency of the gold nanoparticles versus that of cellular
structures, accurate localization of a gold marker is obtained within a 3D
mapping of the entire sample's scattered field, with a lateral precision of 5
nm and 100 nm in the x,y and in the z directions respectively, demonstrating
the ability of holographic microscopy to locate nanoparticles in living cells
environments
Inverse problems with partial data for a magnetic Schr\"odinger operator in an infinite slab and on a bounded domain
In this paper we study inverse boundary value problems with partial data for
the magnetic Schr\"odinger operator. In the case of an infinite slab in ,
, we establish that the magnetic field and the electric potential can
be determined uniquely, when the Dirichlet and Neumann data are given either on
the different boundary hyperplanes of the slab or on the same hyperplane. This
is a generalization of the results of [41], obtained for the Schr\"odinger
operator without magnetic potentials. In the case of a bounded domain in ,
, extending the results of [2], we show the unique determination of the
magnetic field and electric potential from the Dirichlet and Neumann data,
given on two arbitrary open subsets of the boundary, provided that the magnetic
and electric potentials are known in a neighborhood of the boundary.
Generalizing the results of [31], we also obtain uniqueness results for the
magnetic Schr\"odinger operator, when the Dirichlet and Neumann data are known
on the same part of the boundary, assuming that the inaccessible part of the
boundary is a part of a hyperplane
Upconversion nanoparticles as intracellular pH messengers
Abstract: Upconversion nanoparticles (UCNPs) should be particularly well suited for measurement inside cells because they can be imaged down to submicrometer dimensions in near real time using fluorescence microscopy, and they overcome problems, such as photobleaching, autofluorescence, and deep tissue penetration, that are commonly encountered in cellular imaging applications. In this study, the performance of an UCNP modified with a pH-sensitive dye (pHAb) is studied. The dye (emission wavelength 580 nm) was attached in a polyethylene imine (PEI) coating on the UCNP and excited via the 540-nm UCNP emission under 980-nm excitation. The UC resonance energy transfer efficiencies at different pHs ranged from 25 to 30% and a Förster distance of 2.56 nm was predicted from these results. Human neuroblastoma SH-SY5Y cells, equilibrated with nigericin H+/K+ ionophore to equalize the intra- and extracellular pH‚ showed uptake of the UCNP-pHAb conjugate particles and, taking the ratio of the intensity collected from the pHAb emission channel (565–630 nm) to that from the UCNP red emission channel (640–680 nm), produced a sigmoidal pH response curve with an apparent pKa for the UCNP-pHAb of ~ 5.1. The UCNP-pHAb were shown to colocalize with LysoBrite dye, a lysosome marker. Drug inhibitors such as chlorpromazine (CPZ) and nystatin (NYS) that interfere with clathrin-mediated endocytosis and caveolae-mediated endocytosis, respectively, were investigated to elucidate the mechanism of nanoparticle uptake into the cell. This preliminary study suggests that pH indicator–modified UCNPs such as UCNP-pHAb can report pH in SH-SY5Y cells and that the incorporation of the nanoparticles into the cell occurs via clathrin-mediated endocytosis. Graphical abstrac
T-Cell Receptor Beta Variable Gene Polymorphism Predicts Immune-Related adverse Events During Checkpoint Blockade Immunotherapy
BACKGROUND: Immune checkpoint inhibitors have revolutionized cancer treatment. However, they are associated with a unique spectrum of side effects, called immune-related adverse events (irAEs), which can cause significant morbidity and quickly progress to severe or life-threatening events if not treated promptly. Identifying predictive biomarkers for irAEs before immunotherapy initiation is therefore a critical area of research. Polymorphisms within the T-cell receptor beta (TCRB) variable (TRBV) gene have been implicated in autoimmune disease and may be mechanistically linked to irAEs. However, the repetitive nature of the TCRB locus and incomplete genome assembly has hampered the evaluation of TRBV polymorphisms in the past.
PATIENTS AND METHODS: We used a novel method for long-amplicon next generation sequencing of rearranged TCRB chains from peripheral blood total RNA to evaluate the link between TRBV polymorphisms and irAEs in patients treated with immunotherapy for cancer. We employed multiplex PCR to create amplicons spanning the three beta chain complementarity-determining regions (CDR) regions to enable detection of polymorphism within the germline-encoded framework and CDR1 and CDR2 regions in addition to CDR3 profiling. Resultant amplicons were sequenced via the Ion torrent and TRBV allele profiles constructed for each individual was correlated with irAE annotations to identify haplotypes associated with severe irAEs (≥ grade 3).
RESULTS: Our study included 81 patients who had irAEs when treated with immunotherapy for cancer. By using principal component analysis of the 81 TRBV allele profiles followed by k-means clustering, we identified six major TRBV haplotypes. Strikingly, we found that one-third of this cohort possessed a TRBV allele haplotype that appeared to be protective against severe irAEs.
CONCLUSION: The data suggest that long-amplicon TCRB repertoire sequencing can potentially identify TRBV haplotype groups that correlate with the risk of severe irAEs. Germline-encoded TRBV polymorphisms may serve as a predictive biomarker of severe irAEs
Dark-field digital holographic microscopy for 3D-tracking of gold nanoparticles
We present a new technique that combines off-axis Digital Holography and Dark
Field Microscopy to track 100nm gold particles diffusing in water. We show that
a single hologram is sufficient to localize several particles in a thick sample
with a localization accuracy independent of the particle position. From our
measurements we reconstruct the trajectories of the particles and derive their
3D diffusion coefficient. Our results pave the way for quantitative studies of
the motion of single nanoparticle in complex media
Allogeneic Stem Cell Transplantation in Mature T Cell and Natural Killer/T Neoplasias: A Registry Study from Spanish GETH/GELTAMO Centers
Despite advances in understanding the biology of mature T and natural killer (NK)/T cell neoplasia, current therapies, even the most innovative ones, are still far from ensuring its cure. The only treatment to date that has been shown to control aggressive T cell neoplasms in the long term is allogeneic stem cell transplantation (alloSCT). We aim to report the results of alloSCT for advanced mature T and NK/T neoplasias performed in centers from our national GELTAMO/GETH (Grupo Español de Linfoma y Trasplante de Médula Ósea/Grupo Español de Trasplante Hematopoyético y Terapia Celular) over the past 25 years. As a secondary objective, we analyzed the results of alloSCT from haploidentical donors. We performed a retrospective analysis of all patients who received an alloSCT in Spanish centers (n = 201) from September 1995 to August 2018. The 2-year overall survival (OS) and disease-free survival (DFS) were 65.5% and 58.2%, respectively. The univariate for OS and DFS showed statistically different hazard ratios for conditioning intensity, response pre-alloSCT, comorbidity index, donor/receptor cytomegalovirus status and Eastern Cooperative Oncology Group (ECOG) pre-alloSCT, but only a better ECOG pre-alloSCT remained significant in the multivariate analysis. There was an increased incidence of relapse in those patients who did not develop chronic graft-versus-host disease (GVHD) and an increased risk of death in those developing moderate to severe acute GVHD. The 1-year nonrelapse mortality was 21.9% and was mainly due to GVHD (30%) and bacterial infections (17%). When comparing unrelated donors with haploidentical donors, we found similar results in terms of OS and DFS. There was, however, a reduction of acute GVHD in the haploidentical group (P = .04) and trend to a reduction of chronic GVHD. In conclusion, alloSCT is the only curative option for most aggressive T cell neoplasias. Haploidentical donors offer similar results to related donors in terms of survival with a reduction of acute GVHD
Referring to multimodal rehabilitation for patients with musculoskeletal disorders – a register study in primary health care
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