Fibroblast response to initial attachment and proliferation on titanium and zirconium surfaces.

Introduction: In recent decades, dental implants have become one of the best options for comprehensive dental restoration; their placement is a multidisciplinary task that requires a solid understanding of biological, periodontal, surgical and prosthetic principles. Objective: The aim of this study was to quantify in vitro the adhesion and proliferation of human gingival fibroblasts’ (HGF) response on titanium (Ti) and zirconia (Zr) surfaces. Methodology: Samples of Ti and Zr were observed under atomic force microscopy (AFM). HGFs were inoculated in each sample to determine adhesion and cell proliferation. The MTT reagent was mixed with DMEM and inoculated in each plate; formazan was dissolved with dimethyl sulfoxide and analyzed at 540 nm in a microplate spectrophotometer. The test was performed with three independent experiments. Data were analyzed with Kolmogorov-Smirnov tests (Lilliefors), Kruskal-Wallis tests and Mann-Whitney test comparisons. Results: Topography of the Zr plates showed greater roughness (Ra= 0.39μm) than Ti (Ra= 0.049μm). Quantification of HGF adhesion was significantly higher (p˂0.05) in Ti, while proliferation showed no statistically significant differences between the groups. Conclusion: It is noteworthy that, even though Ti initially showed increased cell adhesion on the surface, after 24 h Zr samples showed similar proliferation; this demonstrates that both surfaces have a comparable biological response

The production of lime in the sierras de Aracena y Aroche and its role in shaping the area’s cultural landscape

Este artículo pone de manifiesto el papel que representa la producción de cal en la configuración del paisaje cultural de las sierras de Aracena y Aroche (Huelva), resaltando su potencial como una actividad con interesantes perspectivas de futuro. Muestra la continuidad histórica de la producción de cal en la zona –utilizada tanto para la obtención de mortero como para enjalbegar paredes– y aporta un inventario de los principales grupos de hornos de cal y otras instalaciones afines dispersas por el territorio. El estudio revela que el uso y producción de cal es visible hoy en la singular arquitectura vernácula de los pueblos y del medio rural de la sierra. Asimismo, el artículo pretende sensibilizar sobre la interrupción de la producción de cal, que pone en peligro unos modelos constructivos únicos e incrementa el riesgo de incendios. El artículo concluye señalando que la valorización del patrimonio cultural vinculado a la producción de cal puede ralentizar esta dinámica regresiva, actuando como un vehículo para impulsar innovadoras iniciativas socioeconómicasThis paper discusses the role played by the production of lime in the conformation of the cultural landscape of the sierras de Aracena y Aroche (Huelva), highlighting its potential as an activity with interesting future prospects. It demonstrates the historical continuity of the production of lime in the area –being used for making mortar and for whitewashing walls– and it provides an inventory of the main groups of lime kilns and other related facilities spread throughout the territory. This study reveals that the use and production of lime is visible today in the unique vernacular architecture of the hill towns and the rural environment of the Sierra. At the same time, this paper also aims to raise awareness about the discontinuity of lime production, which jeopardizes the area’s unique building patterns and increases the risk of wildfires. The paper concludes by pointing out that the valorisation of the cultural heritage embodied in the culture of lime making can slow down this regressive dynamic, acting as a vehicle for far-reaching and innovative socioeconomic initiative

Jóvenes rurales y empleo en Tlaxcala, México: trayectorias inciertas

Resumen: El objetivo de este artículo es describir e interpretar algunos de los procesos a través de los cuales las personas que habitan el medio rural se incorporan al mercado de trabajo desde edad temprana y trazan trayectorias caracterizadas por un alto grado de flexibilidad, incertidumbre e improvisación. La investigación se desarrolló en el estado mexicano de Tlaxcala entre 2013 y 2016, con metodologías propias de las ciencias sociales. Se discute cómo las prácticas cotidianas de los jóvenes incorporan disposiciones laborales, que dan lugar a un adulto trabajador-emprendedor precarizado en un marco de ausencia de oportunidades.Abstract: In this paper, the authors describe and interpret some of the processes whereby rural dwellers join the labor market at an early age and follow paths characterized by a high degree of flexibility, uncertainty and improvisation. The research was conducted in the Mexican state of Tlaxcala between 2013 and 2016, using social science methodologies. They discuss how the everyday practices of young people incorporate work arrangements, giving rise to an adult worker/entrepreneur in a precarious situation within a social framework that lacks opportunities.Este trabajo es resultado del proyecto “Ruralidades, sujetos sociales y respuestas comunitarias en el valle Puebla-Tlaxcala” (financiamiento papii t-unam in 300115)

Primary mesodermal dysgenesis of the cornea (Peter´s anomaly)

Primary mesodermal dysgenesis of the cornea, also known as Peter´s anomaly (PA) or keratolenticular dysgenesis, is a rare congenital eye condition caused by an abnormal development of the anterior segment. PA is characterized by unilateral or bilateral corneal opacity (leucoma), that appears since the early neonatal period. The incidence of PA in the United States of America is approximately 1.5 per 100,000 live births. PA is known as Peters-plus syndrome when it presents with systemic malformations. In this article we describe the clinical presentation of an 18-year-old patient with PA that arrived to medical consultation due to bilateral corneal opacities since birth. The patient´s parents refer that he has hypoacusis and deny other systemic pathologies. Clinical exploration reveals a visual acuity of 20/30 of the right eye and 20/100 of the left eye. According to the clinical findings and the absence of systemic anomalies, the patient was diagnosed with PA type II

Light-induced ferroelectric modulation of p-n homojunctions in monolayer MoS2

The association of 2D materials and ferroelectrics offers a promisingapproach to tune the optoelectronic properties of atomically thin TransitionMetal Dichalcogenides (TMDs). In this work, the combined effect offerroelectricity and light on the optoelectronic properties of monolayer(1L)-MoS2 deposited on periodically poled lithium niobate crystals is explored.Using scanning micro-photoluminescence, the effect of excitation intensity,scanning direction, and domain walls on the 1L-MoS2 photoluminescenceproperties is analyzed, offering insights into charge modulation of MoS2 . Thefindings unveil a photoinduced charging process dependent on theferroelectric domain orientation, in which light induces charge generation andtransfer at the monolayer-substrate interface. This highlights the substantialrole of light excitation in ferroelectrically-driven electrostatic doping in MoS 2 .Additionally, the work provides insights into the effect of the strong,nanometrically confined electric fields on LiNbO3 domain wall surfaces,demonstrating precise control over charge carriers in MoS2 , and enabling thecreation of deterministic p-n homojunctions with exceptional precision. Theresults suggest prospects for novel optoelectronic and photonic applicationinvolving monolayer TMDs by combining light-matter interaction processesand the surface selectivity provided by ferroelectric domain structuresPID2019-108257GB-I00, PID2022-137444NB-I00, TED2021-132219A-I00, PID2022-142331NB-I00, PID2019-108257GB-I00, PID2019-106268GB-C31, PID2022-138908NB-C3

New Trends for the Processing of Poly(Methyl Methacrylate) Biomaterial for Dental Prosthodontics

Rehabilitation of masticatory function in patients with absent teeth with removable dentures is an established form of treating partial or complete dentition in edentulous patients. The developments in recent decades with dental implants dominate current dental research. However, medical contraindications, a negative attitude toward implants, or financial limitations on the part of the patients limit their universal applicability, so the rehabilitation with dental prostheses still makes up a significant portion of everyday clinical practice. Conversely, removable dentures are used in the critical conditions of the oral cavity. There are about 500 strains of microorganisms in the mouth, which form the biofilm in an acidic environment causing several issues, such as denture stomatitis, deterioration of the periodontal status of the remaining teeth, or carious lesions in the supporting teeth. Therefore, it is very important to choose a suitable material for the prosthesis. Poly(methyl methacrylate) (PMMA) is an acrylic resin usually used with a long tradition for prosthetic purposes. The aim of this chapter is to present the trends for the processing of PMMA. It includes the chemical synthesis, conventional thermal processing of this acrylic resin, the new processing technique assisted with ultrasound, the antibacterial effect on PMMA with nanoparticles, and the cytotoxicity, genotoxicity, and mutagenesis of this material

Melanogaster coccolobae sp. nov. (Paxillaceae, Boletales), un hongo hipogeo tropical de las áreas urbanas de Quintana Roo, México

Background and Aims: The genus Melanogaster is characterized by its hypogeous to semi hypogeous habit, brownish basidiomata, gel-filled gleba locules, and globose to ellipsoid basidiospores. The genus is distributed in temperate zones, but sequences from Coccoloba root tips and a few basidiome collections have revealed its presence in the tropics. The aim of this article is to describe a new species of Melanogaster based on ecological, molecular, and morphological data. Methods: Specimens were collected in urban vegetation of Quintana Roo in the Yucatán Peninsula, Mexico. For morphological description, the classic protocols for sequestrate fungi were followed. The dried material was deposited in the mycological herbarium “José Castillo Tovar” of the Instituto Tecnológico de Ciudad Victoria (ITCV) and the herbarium of the Universidad Autónoma de Yucatán (UADY). Key results: Melanogaster coccolobae is presented as a new species from the urban gardens of Quintana Roo based on ecological, molecular, and morphological evidence. This species is characterized by its hypogeous to semi hypogeous basidioma, greyish orange, brown to reddish brown peridium composed of two layers, sweet smell, subglobose, ellipsoid or piriform basidiospores, and by its mycorrhizal association with Coccoloba spicata. Conclusions: Melanogaster coccolobae is the first species described from the Mexican Caribbean from urban gardens with Coccoloba spicata. More studies about the tropical sequestrate fungi are recommended.Antecedentes y Objetivos: El género Melanogaster se caracteriza por su hábito hipogeo a semi hipogeo, basidiomas parduscos, gleba con lóculos llenos de gel y basidiosporas globosas a elipsoides. El género se distribuye en zonas templadas, pero secuencias de ectomicorrizas de Coccoloba y pocas colecciones de basidiomas han revelado su presencia en los trópicos. El objetivo de este artículo es describir una nueva especie de Melanogaster a partir de datos ecológicos, moleculares y morfológicos. Métodos: Los especímenes fueron recolectados en jardines urbanos de Quintana Roo en la Península de Yucatán, México. Para la descripción morfológica se siguieron los protocolos clásicos para hongos secuestrados. El material se depositó en el herbario micológico “José Castillo Tovar” del Instituto Tecnológico de Ciudad Victoria (ITCV) y en el herbario de la Universidad Autónoma de Yucatán (UADY). Resultados clave: Melanogaster coccolobae se presenta como una nueva especie de los jardines urbanos de Quintana Roo con base en evidencia morfológica, ecológica y molecular. Esta especie se caracteriza por sus basidiomas hipogeos a semi hipogeos, peridio naranja grisáceo, marrón o marrón rojizo, compuesto por dos capas, olor dulce, basidiosporas subglobosas, elipsoides o piriformes y por formar asociación micorrízica con Coccoloba spicata. Conclusiones: Melanogaster coccolobae es la primera especie descrita del Caribe mexicano en jardines urbanos con Coccoloba spicata. Se recomiendan más estudios sobre los hongos secuestrados tropicales

Differences in ex-vivo Chemosensitivity to Anthracyclines in First Line Acute Myeloid Leukemia

Induction schedules in acute myeloid leukemia (AML) are based on combinations of cytarabine and anthracyclines. The choice of the anthracycline employed has been widely studied in multiple clinical trials showing similar complete remission rates. Using an ex vivo test we have analyzed if a subset of AML patients may respond differently to cytarabine combined with idarubicin, daunorubicin or mitoxantrone. Bone marrow (BM) samples of 198 AML patients were incubated for 48 hours in 96 well plates, each well containing different drugs or drug combinations at different concentrations. Ex vivo drug sensitivity analysis was made using the PharmaFlow platform maintaining the BM microenvironment. Drug response was evaluated as depletion of AML blast cells in each well after incubation. Annexin V-FITC was used to quantify the ability of the drugs to induce apoptosis, and pharmacological responses were calculated using pharmacokinetic population models. Similar dose-respond graphs were generated for the three anthracyclines, with a slight decrease in EC with idarubicin (p=1.462E-06), whereas the interpatient variability of either drug was large. To identify those cases of selective sensitivity to anthracyclines, potency was compared, in terms of area under the curve. Differences in anthracycline monotherapy potency greater than 30% from 3 pairwise comparisons were identified in 28.3% of samples. Furthermore, different sensitivity was detected in 8.2% of patients comparing combinations of cytarabine and anthracyclines. A third of the patients could benefit from the use of this test in the first line induction therapy selection, although it should be confirmed in a clinical trial specifically designed