Desarrollo de estándares de aprendizaje del Español como lengua extranjera nivel B1-B2 en el Estado de Virginia, Estados Unidos.

En nuestra investigación en un total de ocho capítulos y siete anexos presentamos el desarrollo, implementación y análisis de los estándares de aprendizaje de Español como Lengua Extranjera nivel B1-B2 en el estado de Virginia, Estados Unidos. En este trabajo nos focalizamos en los cambios experimentados en un sistema de enseñanza de ELE, en Virginia (Estados Unidos), renovado y transferido a un sistema basado en estándares de aprendizaje. Presentamos, por un lado, los resultados de una investigación experimental en la que se ha trabajado con los dos tipos de currículos, y, por otro lado, el grado de aceptación del currículo basado en estándares entre la comunidad educativa de Virginia. Tomando como base el MCER, PCIC, ACTFL y los expertos que son referenciados en nuestro trabajo, se lleva a cabo esta obra que tiene le cometido de ayudar a docentes y estudiantes a transitar el proceso de enseñanza-aprendizaje de ELE basados en el enfoque comunicativo de la lengua.In our research, in a total of eight chapters and seven annexes, we present the development; implementation; and analysis of the learning standards of Spanish as a Foreign Language level B1-B2 in the state of Virginia, United States. In this paper, we focus on the changes experienced in an ELE teaching system in Virginia (United States), renewed and transferred to a system based on learning standards. We present, on the one hand, the results of an experimental investigation in which we have worked with the two types of curricula; and, on the other hand, the degree of acceptance of the standards-based curriculum among the educational community of Virginia. Based on the CEFR, PCIC, ACTFL and the experts that are referenced in our work, this work is carried out with the purpose of helping teachers and students navigate the teaching learning process of ELE based on the communicative approach of the language.Tesis Univ. Jaén. Departamento de Filología Española. Leída el 15 de marzo de 2019

Silicon nanowires: where mechanics and optics meet at the nanoscale

Mechanical transducers based on nanowires promise revolutionary advances in biological sensing and force microscopy/spectroscopy. A crucial step is the development of simple and non-invasive techniques able to detect displacements with subpicometer sensitivity per unit bandwidth. Here, we design suspended tapered silicon nanowires supporting a range of optical resonances that confine and efficiently scatter light in the visible range. Then, we develop an optical method for efficiently coupling the evanescent field to the regular interference pattern generated by an incoming laser beam and the reflected beam from the substrate underneath the nanowire. This optomechanical coupling is here applied to measure the displacement of 50 nm wide nanowires with sensitivity on the verge of 1 fm/Hz1/2 at room temperature with a simple laser interferometry set-up. This method opens the door to the measurement of the Brownian motion of ultrashort nanowires for the detection of single biomolecular recognition events in liquids, and single molecule spectroscopy in vacuum.We acknowledge financial support from the Spanish Science Ministry (MINECO) through projects TEC2011-29120-C05-04; and from European Research Council through Starting Grant NANOFORCELLS (ERC-StG-2011-278860).Peer reviewe

Prediction of topsoil organic carbon using airborne and satellite hyperspectral imagery

The Airborne Hyperspectral Scanner (AHS) and the Hyperion satellite hyperspectral sensors were evaluated for their ability to predict topsoil organic carbon (C) in burned mountain areas of northwestern Spain slightly covered by heather vegetation. Predictive models that estimated total organic C (TOC) and oxidizable organic C (OC) content were calibrated using two datasets: a ground observation dataset with 39 topsoil samples collected in the field (for models built using AHS data), and a dataset with 200 TOC/OC observations predicted by AHS (for models built using Hyperion data). For both datasets, the prediction was performed by stepwise multiple linear regression (SMLR) using reflectances and spectral indices (SI) obtained from the images, and by the widely-used partial least squares regression (PLSR) method. SMLR provided a performance comparable to or even better than PLSR, while using a lower number of channels. SMLR models for the AHS were based on a maximum of eight indices, and showed a coefficient of determination in the leave-one-out cross-validation R2 = 0.60–0.62, while models for the Hyperion sensor showed R2 = 0.49–0.61, using a maximum of 20 indices. Although slightly worse models were obtained for the Hyperion sensor, which was attributed to its lower signal-to-noise ratio (SNR), the prediction of TOC/OC was consistent across both sensors. The relevant wavelengths for TOC/OC predictions were the red region of the spectrum (600–700 nm), and the short wave infrared region between ~2000–2250 nm. The use of SMLR and spectral indices based on reference channels at ~1000 nm was suitable to quantify topsoil C, and provided an alternative to the more complex PLSR method

Development of nanomechanical biosensors for the determination of cell mechanical properties

Póster presentado en el GEM4 Summer school, celebrado en Londres del 10 al 14 de septiembre de 2012.Peer Reviewe

Optomechanical devides for mechanobiological fingerprinting

Resumen del trabajo presentado en el Frontiers of Nanomechanical Systems (FSN2021), celebraod de forma virtual del 19 al 21 de enero de 2021Twenty years have passed since the first detection of biomolecular recognition using micromechanical systems[1]. In the last two decades, micro- nanomechanical systems have been refined to achieve amazing detection limits in force and mass that have enabled different schemes for ultrasensitive measurements of biological interactions as well as new ways of biological spectrometry. More recently, these figures of merit have been improved by coupling optical cavities to mechanical systems. In this talk, I will review the use of micro- nanomechanical systems for mechanobiological fingerprinting of biological entities, particularizing in the contributions of our group [2]. An essential core of this topic is the discussion about the mechanical coupling between a biological particle and a mechanical resonator, an issue that it is has been often oversimplified. We show that the biomechanical coupling that emerges between a bioparticle and a mechanical resonator is more complex than previously expect and it can give rise to different interaction regimes, in which the resonator response is dominated by different physical parameters of the analyte [3-4]. In particular, we will show experiments done with a variety of micro- nano- optomechanical systems using different measurement schemes where the mass, the stiffness and even the vibration modes of single biological entities can be measured with high sensitivity. It is now widely appreciated the essential role of mechanics in relevant biological processes and how disease can be revealed as changes in the mechanical properties of biological matter. I am pretty sure that future developments in optomechanical devices will contribute for major understanding of diseases as well as for new avenues in diagnosis and therapy

High-Throughput Mass Measurement Of Single Bacterial Cells By Silicon Nitride Membrane Resonators

Trabajo presentado en la 36th International Conference on Micro Electro Mechanical Systems (MEMS), celebrada en Munich (Alemania), del 15 al 19 de enero de 2023.© 2023 IEEE. Personal use of this material is permitted. Permission from IEEE must be obtained for all other uses, in any current or future media, including reprinting/republishing this material for advertising or promotional purposes, creating new collective works, for resale or redistribution to servers or lists, or reuse of any copyrighted component of this work in other works.We present a technological approach to precisely measure the dry mass of many individual cells of a bacteria colony. In this technique, bacteria are transported from aqueous solution into gas phase and subsequently guided to the surface of a silicon nitride membrane resonator. Abrupt downshifts in the membrane eigenfrequencies are measured upon every bacterium adhesion and are related to the dry mass of the cell by theoretical methods. We measure the dry mass of Escherichia coli K-12 and Staphylococcus epidermidis with an unprecedented throughput of 20 cells/min and with a mass resolution of ⁓1%. Finally, we apply the Koch & Schaechter model to assess the intrinsic sources of growth stochasticity.This work was supported by the European Union’s Horizon 2020 Research and Innovation Program under Grant Agreement No. 731868-VIRUSCAN and by the ERC CoG Grant 681275 “LIQUIDMASS”. We acknowledge the service from the Micro and Nanofabrication Laboratory an X-SEM laboratory at IMNCNM funded by the Comunidad de Madrid (Project S2018/NMT-4291 TEC2SPACE) and by MINECO (Project CSIC12-4E-1794 with support from FEDER, FSE). E. G. S. acknowledges financial support by the Spanish Science and Innovation Ministry through Ramón y Cajal grant RYC-2019-026626-I

Development and Validation of Hepamet Fibrosis Scoring System A Simple, Noninvasive Test to Identify Patients With Nonalcoholic Fatty Liver Disease With Advanced Fibrosis

Background & Aims Fibrosis affects prognoses for patients with nonalcoholic fatty liver disease (NAFLD). Several non-invasive scoring systems have aimed to identify patients at risk for advanced fibrosis, but inconclusive results and variations in features of patients (diabetes, obesity and older age) reduce their diagnostic accuracy. We sought to develop a scoring system based on serum markers to identify patients with NAFLD at risk for advanced fibrosis. Methods We collected data from 2452 patients with NAFLD at medical centers in Italy, France, Cuba, and China. We developed the Hepamet fibrosis scoring system using demographic, anthropometric, and laboratory test data, collected at time of liver biopsy, from a training cohort of patients from Spain (n = 768) and validated the system using patients from Cuba (n = 344), Italy (n = 288), France (n = 830), and China (n = 232). Hepamet fibrosis score (HFS) were compared with those of previously developed fibrosis scoring systems (the NAFLD fibrosis score [NFS] and FIB-4). The diagnostic accuracy of the Hepamet fibrosis scoring system was assessed based on area under the receiver operating characteristic (AUROC) curve, sensitivity, specificity, diagnostic odds ratio, and positive and negative predictive values and likelihood ratios. Results Variables used to determine HFS were patient sex, age, homeostatic model assessment score, presence of diabetes, levels of aspartate aminotransferase, and albumin, and platelet counts; these were independently associated with advanced fibrosis. HFS discriminated between patients with and without advanced fibrosis with an AUROC curve value of 0.85 whereas NFS or FIB-4 did so with AUROC values of 0.80 (P = .0001). In the validation set, cut-off HFS of 0.12 and 0.47 identified patients with and without advanced fibrosis with 97.2% specificity, 74% sensitivity, a 92% negative predictive value, a 76.3% positive predictive value, a 13.22 positive likelihood ratio, and a 0.31 negative likelihood ratio. HFS were not affected by patient age, body mass index, hypertransaminasemia, or diabetes. The Hepamet fibrosis scoring system had the greatest net benefit in identifying patients who should undergo liver biopsy analysis and led to significant improvements in reclassification, reducing the number of patients with undetermined results to 20% from 30% for the FIB-4 and NFS systems (P < .05). Conclusions Using clinical and laboratory data from patients with NAFLD, we developed and validated the Hepamet fibrosis scoring system, which identified patients with advanced fibrosis with greater accuracy than the FIB-4 and NFS systems. the Hepamet system provides a greater net benefit for the decision-making process to identify patients who should undergo liver biopsy analysis

Hospitales verdes : un nuevo desafío para América Latina

286 páginas : tablas, gráficos ; 28 cm.Con el presente libro, de una manera práctica y gracias a los autores, se ha querido llevar a los lectores una visión amplia y clara del tema de los hospitales verdes. Los temas que a continuación están desarrollados presentan la base fundamental de lo que es un hospital verde, desde un panorama amplio de los hospitales verdes, las características que debe tenerse en cuenta en el momento de la construcción de uno de estos hospitales, su impacto en la salud, así como experiencias exitosas de estos. El lector encontrará un libro de consulta para adentrarse en el tema de los hospitales verdes saludables, el cual podrá servirle de guía para ampliar su conocimiento sobre el asunto y cómo este concepto de contribuye al mejoramiento del medio ambiente y la conservación del mismo.Panorama de la salud ambiental hospitalaria y política ambiental en instituciones de salud. --Hospitales sustentables: criterios generales para el diseño. -- Hospitales sustentables: construcción y operación. -- Hospitales verdes: ¿aporte real a la salud pública?. -- La cultura verde como estrategia para la gestión ambiental de los hospitales. -- Relación de las acciones de los hospitales verdes en el marco de las dimensiones del desarrollo sostenible. -- Análisis de experiencias de gestión hídrica en las instituciones vinculadas a la Red Global de Hospitales Verdes en Colombia. -- Posicionamiento ambiental de la Red Hospitalaria Española y su relación con Salud sin Daño: hospitales verdes y saludables. -- Proyecto Eco-hospital Guadarrama, España. -- Acciones de cultivos saludables reportadas por los miembros de la Red Global de Hospitales Verdes y Saludables. -- Avances de la vinculación del componente ambiental y hospitales verdes en carreras de ciencias de la salud