Metabolism of homocysteine, its relation to the other cellular thiols and its mechanism of cell damage in a cell culture line (human histiocytic cell line U-937)

AbstractThis study shows that the intracellular concentration of homocysteine in cultured cells is kept low due to an accumulation in the medium. The intracellular level of homocysteine was decreased when its precursor, methionine, was omitted from the culture medium. Intracellular glutathione and cysteine were lowered in cystine-deficient medium. Intracellular glutathione was also lowered when copper ions were added to the culture medium. It is evident from this study that the intracellular concentration of homocysteine was not influenced by the lowered level of glutathione and/or cysteine. High amounts of homocysteine added to the medium give rise to an increase of intracellular reduced homocysteine, which participates in the transsulfuration pathway and can replace cysteine in the synthesis of gluthathione. The addition of relatively high amounts of reduced homocysteine (500 μmol/l) in the presence of copper ions (100 μmol/l) to the culture medium can be directly toxic to the cells, possibly due to oxygen radicals formed by thiol auto-oxidation. Whilst the level of homocysteine in this study using short-time cell culture experiment is much higher than the mild hyperhomocysteinemia thought to be atherogenic in humans, it is conceivable that over a longer time course these levels of homocysteine could be sufficient to induce endothelial dysfunction, eventually leading to atherosclerosis

Market Orientation and CSR: Performance Implications

Corporate social responsibility (CSR) has become of great interest to both researchers and practitioners alike with much discussion on whether the costs outweigh the performance implications. CSR has become a firm strategic tool (not only an ethical concept) as firms recognize that the customer value proposition and CSR is integrated with the focus on how to differentiate the firm from the view of the customer. We utilized market orientation (MO) theory as our foundation for our research as it explains how organizations adapt to their customer environment to develop competitive advantages. With the current customer focus on CSR, MO assists the field in identifying a possible firm differentiation. Our research found that firms that ranked high on CSR correlated positively to performance. We also found our theoretically developed constructs of firm customer orientation (CO) and firm market orientation correlated with the firm adopting CSR. The results also indicated that CSR positively mediates CO and MO to firm performance. As past research had mixed results over the direct relation of MO to performance, our research suggests that CSR may be the missing variable to explain the MO/Performance relationship. © 2015, Springer Science+Business Media Dordrecht

A measurement of the 4He(g,n) reaction from 23 < Eg < 70 MeV

A comprehensive set of 4He(g,n) absolute cross-section measurements has been performed at MAX-lab in Lund, Sweden. Tagged photons from 23 < Eg < 70 MeV were directed toward a liquid 4He target, and neutrons were identified using pulse-shape discrimination and the Time-of-flight Technique in two liquid-scintillator detector arrays. Seven-point angular distributions have been measured for fourteen photon energies. The results have been subjected to complementary Transition-coefficient and Legendre-coefficient analyses. The results are also compared to experimental data measured at comparable photon energies as well as Recoil-Corrected Continuum Shell Model, Resonating Group Method, and Effective Interaction Hyperspherical-Harmonic Expansion calculations. For photon energies below 29 MeV, the angle-integrated data are significantly larger than the values recommended by Calarco, Berman, and Donnelly in 1983.Comment: 16 pages, 14 figures, some more revisions, submitted to Physical Review

Pion emission in 2H, 12C, 27Al, gamma pi+ reactions at threshold

The first data from MAX-lab in Lund, Sweden on pion production in photonuclear reactions at threshold energies, is presented. The decrease of the total yield of pi+ in gamma + 12C, 27Al reactions below 200 MeV as well as differential, dsigma/dOmega, cross sections follow essentially predictions from an intranuclear cascade model with an attractive potential for pion-nucleus interaction in its simplest form. Double differential, d2sigma/dOmegadT, cross sections at 176 MeV show, however, deviations from the model, which call for refinements of nuclear and Coulomb potentials and possibly also for coherent pion production mechanisms.Comment: 19 pages, 7 figure

A measurement of the differential cross section for the two-body photodisintegration of 3He at theta_LAB = 90deg using tagged photons in the energy range 14 -- 31 MeV

The two-body photodisintegration of 3He has been investigated using tagged photons with energies from 14 -- 31 MeV at MAX-lab in Lund, Sweden. The two-body breakup channel was unambiguously identified by the (nonsimultaneous) detection of both protons and deuterons. This approach was made feasible by the over-determined kinematic situation afforded by the tagged-photon technique. Proton- and deuteron-energy spectra were measured using four silicon surface-barrier detector telescopes located at a laboratory angle of 90deg with respect to the incident photon-beam direction. Average statistical and systematic uncertainties of 5.7% and 6.6% in the differential cross section were obtained for 11 photon-energy bins with an average width of 1.2 MeV. The results are compared to previous experimental data measured at comparable photon energies as well as to the results of two recent Faddeev calculations which employ realistic potential models and take into account three-nucleon forces and final-state interactions. Both the accuracy and precision of the present data are improved over the previous measurements. The data are in good agreement with most of the previous results, and favor the inclusion of three-nucleon forces in the calculations.Comment: 12 pages, 13 figures; further Referee comments addresse

Near-threshold measurement of the 4He(g,n) reaction

A near-threshold 4He(g,n) cross-section measurement has been performed at MAX-lab. Tagged photons from 23 < Eg < 42 MeV were directed toward a liquid 4He target, and neutrons were detected by time-of-flight in two liquid-scintillator arrays. Seven-point angular distributions were measured for eight photon energies. The results are compared to experimental data measured at comparable energies and Recoil-Corrected Continuum Shell Model, Resonating Group Method, and recent Hyperspherical-Harmonic Expansion calculations. The angle-integrated cross-section data is peaked at a photon energy of about 28 MeV, in disagreement with the value recommended by Calarco, Berman, and Donnelly in 1983.Comment: 10 pages, 3 figures, some revisions, submitted to Physics Letters

Influence on Plasma β-N-Acetyl-D-glucosaminidase of Reticuloendothelial Stimulation and Depression: An Experimental Study in Rats

Peer Reviewe

New Measurement of Compton Scattering from the Deuteron and an Improved Extraction of the Neutron Electromagnetic Polarizabilities

The electromagnetic polarizabilities of the nucleon are fundamental properties that describe its response to external electric and magnetic fields. They can be extracted from Compton-scattering data --- and have been, with good accuracy, in the case of the proton. In contradistinction, information for the neutron requires the use of Compton scattering from nuclear targets. Here we report a new measurement of elastic photon scattering from deuterium using quasimonoenergetic tagged photons at the MAX IV Laboratory in Lund, Sweden. These first new data in more than a decade effectively double the world dataset. Their energy range overlaps with previous experiments and extends it by 20 MeV to higher energies. An analysis using Chiral Effective Field Theory with dynamical \Delta(1232) degrees of freedom shows the data are consistent with and within the world dataset. After demonstrating that the fit is consistent with the Baldin sum rule, extracting values for the isoscalar nucleon polarizabilities and combining them with a recent result for the proton, we obtain the neutron polarizabilities as \alpha_n = [11.55 +/- 1.25(stat) +/- 0.2(BSR) +/- 0.8(th)] X 10^{-4} fm^3 and \beta_n = [3.65 -/+ 1.25(stat) +/- 0.2(BSR) -/+ 0.8(th)] X 10^{-4} fm3, with \chi^2 = 45.2 for 44 degrees of freedom.Comment: 6 pages, 3 figures, comments from Physical Review Letters Referees addresse

Does reproduction cause oxidative stress? An open question

There has been substantial recent interest in the possible role of oxidative stress as a mechanism underlying life-history trade-offs, particularly with regard to reproductive costs. Several recent papers have found no evidence that reproduction increases oxidative damage and so have questioned the basis of the hypothesis that oxidative damage mediates the reproduction–lifespan trade-off. However, we suggest here that the absence of the predicted relationships could be due to a fundamental problem in the design of all of the published empirical studies, namely a failure to manipulate reproductive effort. We conclude by suggesting experimental approaches that might provide a more conclusive test of the hypothesis