Effects of laparoscopic sleeve gastrectomy on serum lncRNA levels in obese patients

Obesity is a disease associated with excessive fat accumulation in the body, which body mass index (BMI) is greater than 30 kg/m. Bariatric surgery technique is one of the most common treatment options for obesity with the advantage of faster weight loss in a short time. lncRNAs play a role in adipogenesis and metabolic diseases, including obesity, type 2 diabetes, cardiovascular disease (CVD), osteoarthritis, and hypertension, so they are significant targets for therapeutic options. In this study, we aimed to determine lncRNAs and specific parameters that show different expressions in the plasma of patients with obesity. We included fifteen patients with BMI greater than 30 kg/m2 before and less than 30 kg/m2 after laparoscopic sleeve gastrectomy (LSG) in the study. Total RNAs, including lncRNAs and other non-coding RNAs, were isolated from plasma samples of patients, and eight lncRNAs (H19, Neat1, HOTAIR, ANRIL,  MALAT1, ATB, SNGH5, UCA1) were quantified by real-time PCR. Gene Ontology, KEGG, and relation of obesity analysis were utilized. Unpaired Student's t-test Pearson correlation analysis was used for statistical analysis. We observed a statistically significant increase in the expression levels of all lncRNAs in the patients with the post-operative BMI change. We have added a new dimension to the biomarker studies related to obesity and the clinical follow-up of patients undergoing LSG surgery. Further studies are required for enlighting the molecular mechanisms

The Role Of Varicocele Treatment In The Management Of Non-Obstructive Azoospermia

The literature on male reproductive medicine is continually expanding, especially regarding the diagnosis and treatment of infertility due to non-obstructive azoospermia. The advent of in vitro fertilization with intracytoplasmic sperm injection has dramatically improved the treatment of male infertility due to non-obstructive azoospermia. Assisted reproduction using testicular spermatozoa has become a treatment of hope for men previously thought to be incapable of fathering a child due to testicular failure., In addition, numerous studies on non-obstructive azoospermia have reported that varicocelectomy not only can induce spermatogenesis but can also increase the sperm retrieval rate; however, the value of varicocelectomy in patients with non-obstructive azoospermia still remains controversial. The purpose of this review is to present an overview of the current status of varicocele repair in men with non-obstructive azoospermia.PubMedWoSScopu

PROTECTION OF ASCORBIC ACID FROM COPPER(II)-CATALYZED OXIDATIVE DEGRADATION IN THE PRESENCE OF FRUIT ACIDS: CITRIC, OXALIC, TARTARIC, MALIC, MALONIC, AND FUMARIC ACIDS

p Vitamin C (ascorbic acid) is sensitive to oxygen and heat, and can be degraded during unsuitable conditions of cooking and preservation methods of food. The nutritional quality of food may be adversely affected due to transition metal-catalyzed oxidative degradation of ascorbic acid. The effect of Cu(II) complexes formed with protective organic acids widely found in fruits on the autoxidation of ascorbic acid was investigated, where ascorbic acid was quantified with the Cupric Reducing Antioxidant Capacity assay. The Cu(II)-catalyzed oxidation at pH 4.5 followed first-order kinetics with respect to ascorbic acid concentration. The rate constants of ascorbic acid oxidation increased with Cu(II) concentration for a fixed level of organic acid. The catalytic oxidation of ascorbic acid was inhibited in the presence of stable Cu(II)-organic acid binary complexes, and accordingly, the inhibitive potency of citric acid (highest) was followed by oxalic acid, malonic acid, malic acid, tartaric acid, and fumaric acid in this order. The rate-limiting step of ascorbic acid oxidation was the formation of a ternary transition complex between Cu(II), hydrogen ascorbate, and carboxylic acid. The inhibitive activity of Cu(II)-ligand complexes increased as the binary complex stability increased. The presence of fruit acids in foodstuffs may help protect nutritional ascorbic acid values by preventing metal-catalyzed oxidation reactions