Application and Realization of Plc Technology in Mechanical Electrical Control Device

Nowadays, both automation and computer technology have been greatly developed, and PLC technology is also widely used in the field of mechanical and electrical control because of the development of these technologies. PLC technology plays an important role in the digital mechanical production. It can not only improve the mechanical and electrical control devices, but also achieve the purpose of mechanical and electrical automation. As a mainstream technology, PLC has high technical level requirements for related configuration and operation. Therefore, the application of PLC technology has a very high implementation value. Researchers are constantly exploring and deepening PLC technology so that they can be efficiently applied to mechanical electrical control devices. Therefore, this paper briefly analyzes and discusses the application and implementation of PLC technology in mechanical and electrical control devices

Combination of shikonin with paclitaxel overcomes multidrug resistance in human ovarian carcinoma cells in a P-gp-independent manner through enhanced ROS generation

Abstract Background Shikonin (SKN), a naphthoquinone compound, is isolated from Chinese herbal medicine Lithospermum root and has been studied as an anticancer drug candidate in human tumor models. This study is designed to investigate whether SKN can sensitize the therapeutic effect of paclitaxel (PTX) in drug-resistant human ovarian carcinoma cells. Methods Human ovarian carcinoma A2780 cell along with the paired PTX-resistant A2780/PTX cells were used. The effects of SKN, PTX or their combination on cell viability were conducted using Sulforhodamine B assay. P-glycoprotein (P-gp) expression was analyzed by flow cytometry after staining with P-gp-FITC anti-body. P-gp activity was determined by a fluorometric MDR assay kit or a rhodamine 123-based efflux assay, respectively. Apoptosis was evaluated by flow cytometry after Annexin V-FITC/PI co-staining. The effect of SKN, PTX or their combination on reactive oxygen species (ROS) generation and expression of pyruvate kinase M2 (PKM2) were investigated using flow cytometry or western blotting, respectively. PKM2 activity was detected by a Pyruvate Kinase Assay Kit. Results SKN/PTX co-treatment led to synergistically enhanced cytotoxicity and apoptosis in PTX-resistant ovarian cancer cells, indicating the circumvention of multidrug resistance (MDR) of PTX by SKN. Further study indicated that the MDR reversal effect of SKN was independent of inhibiting activity of the efflux transporter P-gp. Notably, SKN/PTX significantly increased the generation of intracellular ROS in A2780/PTX cells, and scavenging intracellular ROS blocked the sensitizing effects of SKN in PTX-induced cytotoxicity and apoptosis in A2780/PTX cells, but not in A2780 cells. Furthermore, SKN/PTX-induced downregulation of PKM2 (a key enzyme in glycolysis) and the suppression of its activity were inhibited by a ROS scavenger N-acetyl cysteine (NAC), suggesting that the synergy of the SKN/PTX combination may be not rely on PKM2 suppression. Conclusions These results reveal a P-gp-independent mechanism through ROS generation for the SKN/PTX combination to overcome MDR in ovarian cancer

Characterization of two thymosins as immune-related genes in common carp (Cyprinus carpio L.)

Prothymosin alpha (ProT alpha) and thymosin beta (T beta) belong to thymosin family, which consists of a series of highly conserved peptides involved in stimulating immune responses. ProT alpha b and T beta are still poorly studied in teleost. Here, the full-length cDNAs of ProT alpha b and T beta-like (T beta-l) were cloned and identified in common carp (Cyprinus carpi L). The expressions of carp ProT alpha b and T beta-l exhibited rise-fall pattern and then trended to be stable during early development. After spring viraemia of carp virus (SVCV) infection, the carp ProT alpha b and T beta-l transcripts were significantly up-regulated in some immune-related organs. When transiently over-expressed carp ProT alpha b and T beta-l in zebrafish, these two proteins upregulated the expressions of T lymphocytes-related genes (Rag 1, TCR-gamma, CD4 and CD8 alpha). These results suggest that carp ProT alpha b and T beta may ultimately enhance the immune response during viral infection and modulate the development of T lymphocytes in teleost. (C) 2015 Elsevier Ltd. All rights reserved